This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.15 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 318,903 (12.1%) Reads that were too short: 284,637 (10.8%) Reads written (passing filters): 34,266 (1.3%) Total basepairs processed: 791,719,999 bp Total written (filtered): 6,755,172 bp (0.9%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 318903 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 279 642.2 0 279 7 112 642.2 0 112 8 142 642.2 0 142 9 228 642.2 0 228 10 9699 642.2 0 9699 11 41 642.2 0 41 12 21 642.2 0 21 13 42 642.2 0 42 14 28 642.2 0 28 15 28 642.2 0 28 16 36 642.2 0 36 17 45 642.2 0 45 18 58 642.2 0 58 19 82 642.2 0 82 20 63 642.2 0 63 21 56 642.2 0 56 22 35 642.2 0 35 23 41 642.2 0 41 24 21 642.2 0 21 25 27 642.2 0 27 26 21 642.2 0 21 27 20 642.2 0 20 28 27 642.2 0 27 29 42 642.2 0 42 30 21 642.2 0 21 31 22 642.2 0 22 32 35 642.2 0 35 33 38 642.2 0 38 34 31 642.2 0 31 35 31 642.2 0 31 36 36 642.2 0 36 37 20 642.2 0 20 38 27 642.2 0 27 39 35 642.2 0 35 40 34 642.2 0 34 41 55 642.2 0 55 42 32 642.2 0 32 43 38 642.2 0 38 44 28 642.2 0 28 45 61 642.2 0 61 46 85 642.2 0 85 47 245 642.2 0 245 48 99 642.2 0 99 49 80 642.2 0 80 50 38 642.2 0 38 51 24 642.2 0 24 52 50 642.2 0 50 53 97 642.2 0 97 54 69 642.2 0 69 55 88 642.2 0 88 56 57 642.2 0 57 57 39 642.2 0 39 58 32 642.2 0 32 59 31 642.2 0 31 60 42 642.2 0 42 61 50 642.2 0 50 62 90 642.2 0 90 63 64 642.2 0 64 64 55 642.2 0 55 65 31 642.2 0 31 66 39 642.2 0 39 67 27 642.2 0 27 68 36 642.2 0 36 69 61 642.2 0 61 70 65 642.2 0 65 71 128 642.2 0 128 72 284 642.2 0 284 73 144 642.2 0 144 74 280 642.2 0 280 75 36 642.2 0 36 76 28 642.2 0 28 77 29 642.2 0 29 78 23 642.2 0 23 79 37 642.2 0 37 80 38 642.2 0 38 81 32 642.2 0 32 82 48 642.2 0 48 83 61 642.2 0 61 84 54 642.2 0 54 85 59 642.2 0 59 86 89 642.2 0 89 87 57 642.2 0 57 88 37 642.2 0 37 89 52 642.2 0 52 90 36 642.2 0 36 91 34 642.2 0 34 92 24 642.2 0 24 93 38 642.2 0 38 94 27 642.2 0 27 95 31 642.2 0 31 96 25 642.2 0 25 97 29 642.2 0 29 98 29 642.2 0 29 99 31 642.2 0 31 100 20 642.2 0 20 101 25 642.2 0 25 102 26 642.2 0 26 103 39 642.2 0 39 104 45 642.2 0 45 105 65 642.2 0 65 106 23 642.2 0 23 107 31 642.2 0 31 108 33 642.2 0 33 109 31 642.2 0 31 110 27 642.2 0 27 111 33 642.2 0 33 112 110 642.2 0 110 113 67 642.2 0 67 114 149 642.2 0 149 115 48 642.2 0 48 116 140 642.2 0 140 117 71 642.2 0 71 118 101 642.2 0 101 119 42 642.2 0 42 120 63 642.2 0 63 121 41 642.2 0 41 122 75 642.2 0 75 123 304 642.2 0 304 124 143 642.2 0 143 125 518 642.2 0 518 126 104 642.2 0 104 127 55 642.2 0 55 128 74 642.2 0 74 129 82 642.2 0 82 130 78 642.2 0 78 131 75 642.2 0 75 132 42 642.2 0 42 133 48 642.2 0 48 134 57 642.2 0 57 135 58 642.2 0 58 136 72 642.2 0 72 137 44 642.2 0 44 138 109 642.2 0 109 139 68 642.2 0 68 140 78 642.2 0 78 141 77 642.2 0 77 142 53 642.2 0 53 143 45 642.2 0 45 144 57 642.2 0 57 145 60 642.2 0 60 146 62 642.2 0 62 147 92 642.2 0 92 148 55 642.2 0 55 149 105 642.2 0 105 150 290 642.2 0 290 151 306 642.2 0 306 152 200 642.2 0 200 153 86 642.2 0 86 154 119 642.2 0 119 155 61 642.2 0 61 156 102 642.2 0 102 157 63 642.2 0 63 158 63 642.2 0 63 159 97 642.2 0 97 160 420 642.2 0 420 161 243 642.2 0 243 162 466 642.2 0 466 163 103 642.2 0 103 164 238 642.2 0 238 165 917 642.2 0 917 166 516 642.2 0 516 167 1233 642.2 0 1233 168 322 642.2 0 322 169 143 642.2 0 143 170 121 642.2 0 121 171 125 642.2 0 125 172 145 642.2 0 145 173 153 642.2 0 153 174 114 642.2 0 114 175 168 642.2 0 168 176 156 642.2 0 156 177 212 642.2 0 212 178 114 642.2 0 114 179 189 642.2 0 189 180 132 642.2 0 132 181 162 642.2 0 162 182 164 642.2 0 164 183 140 642.2 0 140 184 143 642.2 0 143 185 1125 642.2 0 1125 186 1006 642.2 0 1006 187 1399 642.2 0 1399 188 568 642.2 0 568 189 434 642.2 0 434 190 102 642.2 0 102 191 90 642.2 0 90 192 121 642.2 0 121 193 128 642.2 0 128 194 129 642.2 0 129 195 225 642.2 0 225 196 338 642.2 0 338 197 276 642.2 0 276 198 294 642.2 0 294 199 284 642.2 0 284 200 274 642.2 0 274 201 175 642.2 0 175 202 122 642.2 0 122 203 84 642.2 0 84 204 113 642.2 0 113 205 79 642.2 0 79 206 86 642.2 0 86 207 107 642.2 0 107 208 83 642.2 0 83 209 96 642.2 0 96 210 370 642.2 0 370 211 83 642.2 0 83 212 115 642.2 0 115 213 126 642.2 0 126 214 105 642.2 0 105 215 150 642.2 0 150 216 164 642.2 0 164 217 343 642.2 0 343 218 642 642.2 0 642 219 684 642.2 0 684 220 1842 642.2 0 1842 221 3700 642.2 0 3700 222 7943 642.2 0 7943 223 4903 642.2 0 4903 224 2661 642.2 0 2661 225 674 642.2 0 674 226 465 642.2 0 465 227 1061 642.2 0 1061 228 440 642.2 0 440 229 404 642.2 0 404 230 300 642.2 0 300 231 392 642.2 0 392 232 645 642.2 0 645 233 777 642.2 0 777 234 1207 642.2 0 1207 235 1752 642.2 0 1752 236 9966 642.2 0 9966 237 90015 642.2 0 90015 238 43366 642.2 0 43366 239 64434 642.2 0 64434 240 3186 642.2 0 3186 241 1040 642.2 0 1040 242 714 642.2 0 714 243 152 642.2 0 152 244 304 642.2 0 304 245 2442 642.2 0 2442 246 4711 642.2 0 4711 247 5854 642.2 0 5854 248 3889 642.2 0 3889 249 1739 642.2 0 1739 250 1062 642.2 0 1062 251 361 642.2 0 361 252 145 642.2 0 145 253 62 642.2 0 62 254 48 642.2 0 48 255 71 642.2 0 71 256 96 642.2 0 96 257 81 642.2 0 81 258 93 642.2 0 93 259 82 642.2 0 82 260 231 642.2 0 231 261 188 642.2 0 188 262 296 642.2 0 296 263 92 642.2 0 92 264 91 642.2 0 91 265 79 642.2 0 79 266 137 642.2 0 137 267 290 642.2 0 290 268 921 642.2 0 921 269 758 642.2 0 758 270 657 642.2 0 657 271 603 642.2 0 603 272 169 642.2 0 169 273 143 642.2 0 143 274 230 642.2 0 230 275 183 642.2 0 183 276 204 642.2 0 204 277 233 642.2 0 233 278 284 642.2 0 284 279 150 642.2 0 150 280 365 642.2 0 365 281 699 642.2 0 699 282 437 642.2 0 437 283 993 642.2 0 993 284 188 642.2 0 188 285 200 642.2 0 200 286 523 642.2 0 523 287 638 642.2 0 638 288 651 642.2 0 651 289 1377 642.2 0 1377 290 814 642.2 0 814 291 841 642.2 0 841 292 439 642.2 0 439 293 262 642.2 0 262 294 972 642.2 0 972 295 571 642.2 0 571 296 927 642.2 0 927 297 383 642.2 0 383 298 206 642.2 0 206 299 204 642.2 0 204 300 150 642.2 0 150 301 227 642.2 0 227 Finished in 1.42 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 34,266 Reads with adapters: 78 (0.2%) Reads that were too short: 33 (0.1%) Reads written (passing filters): 45 (0.1%) Total basepairs processed: 6,755,172 bp Total written (filtered): 10,115 bp (0.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 78 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 2 0.0 3 0 0 0 1 1 20 5 0.0 4 2 2 1 21 16 0.0 4 10 5 0 1 22 9 0.0 4 0 0 0 0 9 23 2 0.0 4 1 0 1 25 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 31 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 3 0.0 4 0 0 0 0 3 55 2 0.0 4 0 0 0 0 2 56 1 0.0 4 0 0 0 0 1 62 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 124 1 0.0 4 1 135 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 209 2 0.0 4 0 0 0 0 2 213 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 1 280 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (222 us/read; 0.27 M reads/minute). === Summary === Total reads processed: 45 Reads with adapters: 5 (11.1%) Reads written (passing filters): 45 (100.0%) Total basepairs processed: 10,115 bp Total written (filtered): 10,060 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 60.0% C: 0.0% G: 40.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.7 0 1 10 2 0.0 1 1 1 11 1 0.0 1 1 21 1 0.0 1 1