This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.17 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 282,667 (10.7%) Reads that were too short: 12,813 (0.5%) Reads written (passing filters): 269,854 (10.3%) Total basepairs processed: 791,719,999 bp Total written (filtered): 42,530,143 bp (5.4%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 282667 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 133 642.2 0 133 7 5535 642.2 0 5535 8 496 642.2 0 496 9 4001 642.2 0 4001 10 90 642.2 0 90 11 41 642.2 0 41 12 57 642.2 0 57 13 79 642.2 0 79 14 111 642.2 0 111 15 124 642.2 0 124 16 75 642.2 0 75 17 36 642.2 0 36 18 49 642.2 0 49 19 56 642.2 0 56 20 58 642.2 0 58 21 50 642.2 0 50 22 46 642.2 0 46 23 46 642.2 0 46 24 95 642.2 0 95 25 91 642.2 0 91 26 209 642.2 0 209 27 1085 642.2 0 1085 28 2404 642.2 0 2404 29 936 642.2 0 936 30 50 642.2 0 50 31 41 642.2 0 41 32 62 642.2 0 62 33 88 642.2 0 88 34 103 642.2 0 103 35 63 642.2 0 63 36 62 642.2 0 62 37 108 642.2 0 108 38 55 642.2 0 55 39 51 642.2 0 51 40 73 642.2 0 73 41 74 642.2 0 74 42 99 642.2 0 99 43 128 642.2 0 128 44 87 642.2 0 87 45 67 642.2 0 67 46 65 642.2 0 65 47 59 642.2 0 59 48 200 642.2 0 200 49 74 642.2 0 74 50 92 642.2 0 92 51 87 642.2 0 87 52 214 642.2 0 214 53 71 642.2 0 71 54 59 642.2 0 59 55 51 642.2 0 51 56 66 642.2 0 66 57 217 642.2 0 217 58 252 642.2 0 252 59 509 642.2 0 509 60 74 642.2 0 74 61 71 642.2 0 71 62 75 642.2 0 75 63 68 642.2 0 68 64 55 642.2 0 55 65 80 642.2 0 80 66 82 642.2 0 82 67 59 642.2 0 59 68 68 642.2 0 68 69 71 642.2 0 71 70 72 642.2 0 72 71 61 642.2 0 61 72 57 642.2 0 57 73 56 642.2 0 56 74 65 642.2 0 65 75 67 642.2 0 67 76 68 642.2 0 68 77 116 642.2 0 116 78 109 642.2 0 109 79 83 642.2 0 83 80 80 642.2 0 80 81 110 642.2 0 110 82 60 642.2 0 60 83 70 642.2 0 70 84 85 642.2 0 85 85 85 642.2 0 85 86 60 642.2 0 60 87 76 642.2 0 76 88 78 642.2 0 78 89 83 642.2 0 83 90 106 642.2 0 106 91 119 642.2 0 119 92 121 642.2 0 121 93 106 642.2 0 106 94 103 642.2 0 103 95 86 642.2 0 86 96 100 642.2 0 100 97 79 642.2 0 79 98 94 642.2 0 94 99 91 642.2 0 91 100 75 642.2 0 75 101 84 642.2 0 84 102 91 642.2 0 91 103 113 642.2 0 113 104 152 642.2 0 152 105 241 642.2 0 241 106 286 642.2 0 286 107 1243 642.2 0 1243 108 11053 642.2 0 11053 109 7501 642.2 0 7501 110 8149 642.2 0 8149 111 138 642.2 0 138 112 75 642.2 0 75 113 74 642.2 0 74 114 78 642.2 0 78 115 118 642.2 0 118 116 128 642.2 0 128 117 132 642.2 0 132 118 117 642.2 0 117 119 87 642.2 0 87 120 90 642.2 0 90 121 103 642.2 0 103 122 93 642.2 0 93 123 69 642.2 0 69 124 75 642.2 0 75 125 137 642.2 0 137 126 86 642.2 0 86 127 148 642.2 0 148 128 475 642.2 0 475 129 147 642.2 0 147 130 310 642.2 0 310 131 113 642.2 0 113 132 143 642.2 0 143 133 204 642.2 0 204 134 1238 642.2 0 1238 135 628 642.2 0 628 136 2403 642.2 0 2403 137 244 642.2 0 244 138 146 642.2 0 146 139 123 642.2 0 123 140 179 642.2 0 179 141 251 642.2 0 251 142 385 642.2 0 385 143 240 642.2 0 240 144 201 642.2 0 201 145 252 642.2 0 252 146 512 642.2 0 512 147 1070 642.2 0 1070 148 322 642.2 0 322 149 1231 642.2 0 1231 150 754 642.2 0 754 151 264 642.2 0 264 152 310 642.2 0 310 153 665 642.2 0 665 154 719 642.2 0 719 155 3478 642.2 0 3478 156 2741 642.2 0 2741 157 2972 642.2 0 2972 158 1160 642.2 0 1160 159 2465 642.2 0 2465 160 36239 642.2 0 36239 161 78014 642.2 0 78014 162 72103 642.2 0 72103 163 454 642.2 0 454 164 136 642.2 0 136 165 161 642.2 0 161 166 102 642.2 0 102 167 93 642.2 0 93 168 72 642.2 0 72 169 91 642.2 0 91 170 74 642.2 0 74 171 70 642.2 0 70 172 87 642.2 0 87 173 63 642.2 0 63 174 83 642.2 0 83 175 136 642.2 0 136 176 111 642.2 0 111 177 73 642.2 0 73 178 91 642.2 0 91 179 134 642.2 0 134 180 148 642.2 0 148 181 138 642.2 0 138 182 88 642.2 0 88 183 123 642.2 0 123 184 81 642.2 0 81 185 61 642.2 0 61 186 69 642.2 0 69 187 80 642.2 0 80 188 78 642.2 0 78 189 74 642.2 0 74 190 73 642.2 0 73 191 118 642.2 0 118 192 116 642.2 0 116 193 158 642.2 0 158 194 71 642.2 0 71 195 82 642.2 0 82 196 71 642.2 0 71 197 81 642.2 0 81 198 98 642.2 0 98 199 75 642.2 0 75 200 66 642.2 0 66 201 58 642.2 0 58 202 114 642.2 0 114 203 119 642.2 0 119 204 83 642.2 0 83 205 73 642.2 0 73 206 74 642.2 0 74 207 72 642.2 0 72 208 95 642.2 0 95 209 91 642.2 0 91 210 61 642.2 0 61 211 71 642.2 0 71 212 72 642.2 0 72 213 57 642.2 0 57 214 73 642.2 0 73 215 71 642.2 0 71 216 76 642.2 0 76 217 77 642.2 0 77 218 101 642.2 0 101 219 109 642.2 0 109 220 122 642.2 0 122 221 190 642.2 0 190 222 473 642.2 0 473 223 285 642.2 0 285 224 210 642.2 0 210 225 364 642.2 0 364 226 88 642.2 0 88 227 74 642.2 0 74 228 56 642.2 0 56 229 67 642.2 0 67 230 99 642.2 0 99 231 95 642.2 0 95 232 129 642.2 0 129 233 130 642.2 0 130 234 165 642.2 0 165 235 136 642.2 0 136 236 130 642.2 0 130 237 69 642.2 0 69 238 66 642.2 0 66 239 76 642.2 0 76 240 83 642.2 0 83 241 103 642.2 0 103 242 90 642.2 0 90 243 120 642.2 0 120 244 101 642.2 0 101 245 58 642.2 0 58 246 70 642.2 0 70 247 76 642.2 0 76 248 69 642.2 0 69 249 74 642.2 0 74 250 136 642.2 0 136 251 361 642.2 0 361 252 288 642.2 0 288 253 408 642.2 0 408 254 995 642.2 0 995 255 437 642.2 0 437 256 313 642.2 0 313 257 113 642.2 0 113 258 79 642.2 0 79 259 68 642.2 0 68 260 100 642.2 0 100 261 156 642.2 0 156 262 147 642.2 0 147 263 146 642.2 0 146 264 128 642.2 0 128 265 97 642.2 0 97 266 81 642.2 0 81 267 87 642.2 0 87 268 59 642.2 0 59 269 142 642.2 0 142 270 134 642.2 0 134 271 125 642.2 0 125 272 105 642.2 0 105 273 87 642.2 0 87 274 58 642.2 0 58 275 79 642.2 0 79 276 71 642.2 0 71 277 84 642.2 0 84 278 158 642.2 0 158 279 140 642.2 0 140 280 115 642.2 0 115 281 139 642.2 0 139 282 207 642.2 0 207 283 220 642.2 0 220 284 140 642.2 0 140 285 78 642.2 0 78 286 68 642.2 0 68 287 59 642.2 0 59 288 47 642.2 0 47 289 62 642.2 0 62 290 82 642.2 0 82 291 124 642.2 0 124 292 95 642.2 0 95 293 95 642.2 0 95 294 61 642.2 0 61 295 51 642.2 0 51 296 77 642.2 0 77 297 62 642.2 0 62 298 53 642.2 0 53 299 80 642.2 0 80 300 80 642.2 0 80 301 79 642.2 0 79 Finished in 9.44 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 269,854 Reads with adapters: 4,599 (1.7%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 4,553 (1.7%) Total basepairs processed: 42,530,143 bp Total written (filtered): 1,229,819 bp (2.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4599 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 2 1 19 28 0.0 3 0 14 5 7 2 20 4401 0.0 4 4024 283 71 18 5 21 10 0.0 4 0 6 2 0 2 22 5 0.0 4 1 1 1 0 2 23 20 0.0 4 0 0 0 14 6 25 1 0.0 4 0 0 0 1 26 1 0.0 4 1 30 2 0.0 4 1 0 0 0 1 35 1 0.0 4 0 0 0 0 1 42 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 1 54 3 0.0 4 0 0 0 0 3 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 3 0.0 4 0 0 0 0 3 77 3 0.0 4 0 0 0 0 3 80 5 0.0 4 0 0 0 1 4 81 10 0.0 4 0 0 0 0 10 82 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 101 3 0.0 4 0 0 0 0 3 104 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 1 113 1 0.0 4 0 0 0 0 1 127 72 0.0 4 0 0 0 0 72 130 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 1 137 1 0.0 4 0 0 0 1 142 3 0.0 4 0 0 0 0 3 150 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 1 165 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 4,553 Reads with adapters: 1,241 (27.3%) Reads written (passing filters): 4,553 (100.0%) Total basepairs processed: 1,229,819 bp Total written (filtered): 1,210,932 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1241 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 10.8% G: 82.4% T: 5.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 71.1 0 2 4 3 17.8 0 3 8 9 0.1 0 9 9 350 0.0 0 53 297 10 701 0.0 1 56 645 11 136 0.0 1 6 130 12 4 0.0 1 0 4 13 3 0.0 1 0 3 135 1 0.0 1 0 1 150 3 0.0 1 2 1 223 27 0.0 1 25 2 224 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.