This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.89 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 238,706 (9.1%) Reads that were too short: 8,058 (0.3%) Reads written (passing filters): 230,648 (8.8%) Total basepairs processed: 791,719,999 bp Total written (filtered): 36,526,054 bp (4.6%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 238706 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 97 642.2 0 97 7 4903 642.2 0 4903 8 547 642.2 0 547 9 5107 642.2 0 5107 10 73 642.2 0 73 11 39 642.2 0 39 12 49 642.2 0 49 13 63 642.2 0 63 14 63 642.2 0 63 15 82 642.2 0 82 16 53 642.2 0 53 17 31 642.2 0 31 18 41 642.2 0 41 19 59 642.2 0 59 20 60 642.2 0 60 21 61 642.2 0 61 22 51 642.2 0 51 23 54 642.2 0 54 24 57 642.2 0 57 25 60 642.2 0 60 26 59 642.2 0 59 27 147 642.2 0 147 28 214 642.2 0 214 29 133 642.2 0 133 30 48 642.2 0 48 31 39 642.2 0 39 32 51 642.2 0 51 33 53 642.2 0 53 34 70 642.2 0 70 35 63 642.2 0 63 36 70 642.2 0 70 37 72 642.2 0 72 38 49 642.2 0 49 39 56 642.2 0 56 40 49 642.2 0 49 41 76 642.2 0 76 42 60 642.2 0 60 43 65 642.2 0 65 44 68 642.2 0 68 45 58 642.2 0 58 46 71 642.2 0 71 47 61 642.2 0 61 48 196 642.2 0 196 49 56 642.2 0 56 50 72 642.2 0 72 51 68 642.2 0 68 52 231 642.2 0 231 53 62 642.2 0 62 54 40 642.2 0 40 55 43 642.2 0 43 56 50 642.2 0 50 57 157 642.2 0 157 58 112 642.2 0 112 59 245 642.2 0 245 60 72 642.2 0 72 61 51 642.2 0 51 62 58 642.2 0 58 63 57 642.2 0 57 64 40 642.2 0 40 65 47 642.2 0 47 66 48 642.2 0 48 67 53 642.2 0 53 68 62 642.2 0 62 69 67 642.2 0 67 70 74 642.2 0 74 71 48 642.2 0 48 72 40 642.2 0 40 73 47 642.2 0 47 74 52 642.2 0 52 75 54 642.2 0 54 76 44 642.2 0 44 77 69 642.2 0 69 78 84 642.2 0 84 79 44 642.2 0 44 80 66 642.2 0 66 81 91 642.2 0 91 82 51 642.2 0 51 83 46 642.2 0 46 84 69 642.2 0 69 85 83 642.2 0 83 86 60 642.2 0 60 87 55 642.2 0 55 88 70 642.2 0 70 89 90 642.2 0 90 90 87 642.2 0 87 91 114 642.2 0 114 92 89 642.2 0 89 93 108 642.2 0 108 94 93 642.2 0 93 95 94 642.2 0 94 96 87 642.2 0 87 97 67 642.2 0 67 98 67 642.2 0 67 99 55 642.2 0 55 100 46 642.2 0 46 101 77 642.2 0 77 102 111 642.2 0 111 103 107 642.2 0 107 104 138 642.2 0 138 105 211 642.2 0 211 106 272 642.2 0 272 107 1053 642.2 0 1053 108 12704 642.2 0 12704 109 8369 642.2 0 8369 110 9055 642.2 0 9055 111 119 642.2 0 119 112 69 642.2 0 69 113 58 642.2 0 58 114 53 642.2 0 53 115 78 642.2 0 78 116 126 642.2 0 126 117 130 642.2 0 130 118 81 642.2 0 81 119 64 642.2 0 64 120 84 642.2 0 84 121 68 642.2 0 68 122 73 642.2 0 73 123 58 642.2 0 58 124 49 642.2 0 49 125 89 642.2 0 89 126 56 642.2 0 56 127 90 642.2 0 90 128 151 642.2 0 151 129 81 642.2 0 81 130 291 642.2 0 291 131 82 642.2 0 82 132 102 642.2 0 102 133 160 642.2 0 160 134 1127 642.2 0 1127 135 618 642.2 0 618 136 2156 642.2 0 2156 137 106 642.2 0 106 138 115 642.2 0 115 139 70 642.2 0 70 140 109 642.2 0 109 141 125 642.2 0 125 142 109 642.2 0 109 143 99 642.2 0 99 144 145 642.2 0 145 145 217 642.2 0 217 146 410 642.2 0 410 147 888 642.2 0 888 148 238 642.2 0 238 149 1234 642.2 0 1234 150 615 642.2 0 615 151 215 642.2 0 215 152 255 642.2 0 255 153 557 642.2 0 557 154 643 642.2 0 643 155 3478 642.2 0 3478 156 2719 642.2 0 2719 157 2926 642.2 0 2926 158 856 642.2 0 856 159 1704 642.2 0 1704 160 27806 642.2 0 27806 161 65261 642.2 0 65261 162 61880 642.2 0 61880 163 261 642.2 0 261 164 85 642.2 0 85 165 86 642.2 0 86 166 57 642.2 0 57 167 76 642.2 0 76 168 54 642.2 0 54 169 67 642.2 0 67 170 63 642.2 0 63 171 49 642.2 0 49 172 55 642.2 0 55 173 57 642.2 0 57 174 78 642.2 0 78 175 59 642.2 0 59 176 59 642.2 0 59 177 70 642.2 0 70 178 44 642.2 0 44 179 52 642.2 0 52 180 78 642.2 0 78 181 62 642.2 0 62 182 55 642.2 0 55 183 45 642.2 0 45 184 40 642.2 0 40 185 56 642.2 0 56 186 44 642.2 0 44 187 50 642.2 0 50 188 62 642.2 0 62 189 41 642.2 0 41 190 58 642.2 0 58 191 82 642.2 0 82 192 63 642.2 0 63 193 76 642.2 0 76 194 49 642.2 0 49 195 57 642.2 0 57 196 39 642.2 0 39 197 34 642.2 0 34 198 51 642.2 0 51 199 41 642.2 0 41 200 44 642.2 0 44 201 46 642.2 0 46 202 74 642.2 0 74 203 80 642.2 0 80 204 54 642.2 0 54 205 59 642.2 0 59 206 43 642.2 0 43 207 47 642.2 0 47 208 65 642.2 0 65 209 57 642.2 0 57 210 40 642.2 0 40 211 56 642.2 0 56 212 41 642.2 0 41 213 41 642.2 0 41 214 47 642.2 0 47 215 45 642.2 0 45 216 57 642.2 0 57 217 45 642.2 0 45 218 68 642.2 0 68 219 63 642.2 0 63 220 57 642.2 0 57 221 86 642.2 0 86 222 216 642.2 0 216 223 179 642.2 0 179 224 133 642.2 0 133 225 202 642.2 0 202 226 60 642.2 0 60 227 69 642.2 0 69 228 68 642.2 0 68 229 48 642.2 0 48 230 61 642.2 0 61 231 55 642.2 0 55 232 78 642.2 0 78 233 96 642.2 0 96 234 105 642.2 0 105 235 81 642.2 0 81 236 99 642.2 0 99 237 67 642.2 0 67 238 72 642.2 0 72 239 37 642.2 0 37 240 52 642.2 0 52 241 62 642.2 0 62 242 67 642.2 0 67 243 63 642.2 0 63 244 80 642.2 0 80 245 51 642.2 0 51 246 52 642.2 0 52 247 56 642.2 0 56 248 48 642.2 0 48 249 57 642.2 0 57 250 85 642.2 0 85 251 443 642.2 0 443 252 207 642.2 0 207 253 274 642.2 0 274 254 274 642.2 0 274 255 139 642.2 0 139 256 83 642.2 0 83 257 42 642.2 0 42 258 58 642.2 0 58 259 49 642.2 0 49 260 56 642.2 0 56 261 86 642.2 0 86 262 90 642.2 0 90 263 88 642.2 0 88 264 60 642.2 0 60 265 63 642.2 0 63 266 51 642.2 0 51 267 65 642.2 0 65 268 48 642.2 0 48 269 178 642.2 0 178 270 79 642.2 0 79 271 77 642.2 0 77 272 91 642.2 0 91 273 45 642.2 0 45 274 47 642.2 0 47 275 62 642.2 0 62 276 48 642.2 0 48 277 57 642.2 0 57 278 126 642.2 0 126 279 141 642.2 0 141 280 87 642.2 0 87 281 81 642.2 0 81 282 107 642.2 0 107 283 134 642.2 0 134 284 86 642.2 0 86 285 63 642.2 0 63 286 45 642.2 0 45 287 59 642.2 0 59 288 38 642.2 0 38 289 38 642.2 0 38 290 81 642.2 0 81 291 91 642.2 0 91 292 47 642.2 0 47 293 39 642.2 0 39 294 52 642.2 0 52 295 40 642.2 0 40 296 66 642.2 0 66 297 53 642.2 0 53 298 49 642.2 0 49 299 43 642.2 0 43 300 47 642.2 0 47 301 61 642.2 0 61 Finished in 7.39 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 230,648 Reads with adapters: 6,274 (2.7%) Reads that were too short: 97 (0.0%) Reads written (passing filters): 6,177 (2.7%) Total basepairs processed: 36,526,054 bp Total written (filtered): 1,484,990 bp (4.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6274 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 16 0.0 3 0 15 1 17 4794 0.0 3 4719 72 2 1 18 10 0.0 3 1 9 19 5 0.0 3 0 0 3 2 20 2 0.0 3 0 0 0 2 28 2 0.0 3 2 30 1 0.0 3 0 0 0 1 31 1233 0.0 3 0 0 2 1231 34 82 0.0 3 0 0 2 80 44 14 0.0 3 0 0 0 14 47 1 0.0 3 0 0 0 1 57 8 0.0 3 0 0 0 8 60 3 0.0 3 0 0 0 3 63 4 0.0 3 0 0 0 4 65 2 0.0 3 1 0 0 1 82 12 0.0 3 0 0 0 12 89 1 0.0 3 1 90 1 0.0 3 1 91 3 0.0 3 0 0 0 3 92 1 0.0 3 1 94 1 0.0 3 0 1 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 1 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 1 128 1 0.0 3 1 132 45 0.0 3 0 0 1 44 133 7 0.0 3 0 0 0 7 134 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 6,177 Reads with adapters: 19 (0.3%) Reads written (passing filters): 6,177 (100.0%) Total basepairs processed: 1,484,990 bp Total written (filtered): 1,484,928 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 19 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.3% C: 36.8% G: 26.3% T: 10.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 16 96.5 0 16 4 2 24.1 0 2 6 1 1.5 0 1