This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGGCCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.76 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 150,437 (5.7%) Reads that were too short: 72,037 (2.7%) Reads written (passing filters): 78,400 (3.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 16,774,663 bp (2.1%) === Adapter 1 === Sequence: CGGCCA; Type: regular 5'; Length: 6; Trimmed: 150437 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2060 642.2 0 2060 7 13673 642.2 0 13673 8 906 642.2 0 906 9 14679 642.2 0 14679 10 102 642.2 0 102 11 1026 642.2 0 1026 12 49 642.2 0 49 13 57 642.2 0 57 14 40 642.2 0 40 15 37 642.2 0 37 16 26 642.2 0 26 17 38 642.2 0 38 18 41 642.2 0 41 19 27 642.2 0 27 20 36 642.2 0 36 21 60 642.2 0 60 22 42 642.2 0 42 23 37 642.2 0 37 24 37 642.2 0 37 25 34 642.2 0 34 26 34 642.2 0 34 27 35 642.2 0 35 28 46 642.2 0 46 29 28 642.2 0 28 30 49 642.2 0 49 31 61 642.2 0 61 32 52 642.2 0 52 33 52 642.2 0 52 34 42 642.2 0 42 35 52 642.2 0 52 36 43 642.2 0 43 37 39 642.2 0 39 38 59 642.2 0 59 39 52 642.2 0 52 40 109 642.2 0 109 41 260 642.2 0 260 42 257 642.2 0 257 43 188 642.2 0 188 44 36 642.2 0 36 45 24 642.2 0 24 46 53 642.2 0 53 47 47 642.2 0 47 48 51 642.2 0 51 49 55 642.2 0 55 50 119 642.2 0 119 51 66 642.2 0 66 52 66 642.2 0 66 53 56 642.2 0 56 54 70 642.2 0 70 55 57 642.2 0 57 56 60 642.2 0 60 57 48 642.2 0 48 58 31 642.2 0 31 59 30 642.2 0 30 60 44 642.2 0 44 61 36 642.2 0 36 62 41 642.2 0 41 63 53 642.2 0 53 64 249 642.2 0 249 65 314 642.2 0 314 66 257 642.2 0 257 67 61 642.2 0 61 68 44 642.2 0 44 69 39 642.2 0 39 70 42 642.2 0 42 71 64 642.2 0 64 72 58 642.2 0 58 73 256 642.2 0 256 74 1749 642.2 0 1749 75 865 642.2 0 865 76 495 642.2 0 495 77 55 642.2 0 55 78 78 642.2 0 78 79 50 642.2 0 50 80 47 642.2 0 47 81 40 642.2 0 40 82 59 642.2 0 59 83 75 642.2 0 75 84 42 642.2 0 42 85 30 642.2 0 30 86 57 642.2 0 57 87 312 642.2 0 312 88 178 642.2 0 178 89 368 642.2 0 368 90 46 642.2 0 46 91 35 642.2 0 35 92 58 642.2 0 58 93 55 642.2 0 55 94 48 642.2 0 48 95 44 642.2 0 44 96 37 642.2 0 37 97 53 642.2 0 53 98 62 642.2 0 62 99 58 642.2 0 58 100 45 642.2 0 45 101 75 642.2 0 75 102 34 642.2 0 34 103 51 642.2 0 51 104 64 642.2 0 64 105 60 642.2 0 60 106 44 642.2 0 44 107 37 642.2 0 37 108 40 642.2 0 40 109 58 642.2 0 58 110 40 642.2 0 40 111 53 642.2 0 53 112 44 642.2 0 44 113 44 642.2 0 44 114 147 642.2 0 147 115 55 642.2 0 55 116 61 642.2 0 61 117 70 642.2 0 70 118 54 642.2 0 54 119 39 642.2 0 39 120 45 642.2 0 45 121 51 642.2 0 51 122 40 642.2 0 40 123 63 642.2 0 63 124 49 642.2 0 49 125 115 642.2 0 115 126 137 642.2 0 137 127 73 642.2 0 73 128 55 642.2 0 55 129 56 642.2 0 56 130 54 642.2 0 54 131 60 642.2 0 60 132 87 642.2 0 87 133 74 642.2 0 74 134 61 642.2 0 61 135 56 642.2 0 56 136 57 642.2 0 57 137 107 642.2 0 107 138 144 642.2 0 144 139 83 642.2 0 83 140 41 642.2 0 41 141 57 642.2 0 57 142 75 642.2 0 75 143 167 642.2 0 167 144 89 642.2 0 89 145 87 642.2 0 87 146 94 642.2 0 94 147 97 642.2 0 97 148 95 642.2 0 95 149 83 642.2 0 83 150 137 642.2 0 137 151 833 642.2 0 833 152 412 642.2 0 412 153 1481 642.2 0 1481 154 303 642.2 0 303 155 853 642.2 0 853 156 4772 642.2 0 4772 157 1769 642.2 0 1769 158 5344 642.2 0 5344 159 1001 642.2 0 1001 160 185 642.2 0 185 161 265 642.2 0 265 162 129 642.2 0 129 163 297 642.2 0 297 164 174 642.2 0 174 165 219 642.2 0 219 166 270 642.2 0 270 167 144 642.2 0 144 168 105 642.2 0 105 169 158 642.2 0 158 170 261 642.2 0 261 171 1723 642.2 0 1723 172 447 642.2 0 447 173 311 642.2 0 311 174 129 642.2 0 129 175 234 642.2 0 234 176 1467 642.2 0 1467 177 2249 642.2 0 2249 178 2220 642.2 0 2220 179 984 642.2 0 984 180 333 642.2 0 333 181 184 642.2 0 184 182 336 642.2 0 336 183 223 642.2 0 223 184 154 642.2 0 154 185 97 642.2 0 97 186 79 642.2 0 79 187 136 642.2 0 136 188 627 642.2 0 627 189 297 642.2 0 297 190 137 642.2 0 137 191 205 642.2 0 205 192 95 642.2 0 95 193 99 642.2 0 99 194 91 642.2 0 91 195 126 642.2 0 126 196 111 642.2 0 111 197 84 642.2 0 84 198 46 642.2 0 46 199 83 642.2 0 83 200 246 642.2 0 246 201 380 642.2 0 380 202 267 642.2 0 267 203 104 642.2 0 104 204 54 642.2 0 54 205 111 642.2 0 111 206 111 642.2 0 111 207 104 642.2 0 104 208 105 642.2 0 105 209 60 642.2 0 60 210 75 642.2 0 75 211 70 642.2 0 70 212 65 642.2 0 65 213 90 642.2 0 90 214 183 642.2 0 183 215 501 642.2 0 501 216 240 642.2 0 240 217 86 642.2 0 86 218 111 642.2 0 111 219 85 642.2 0 85 220 99 642.2 0 99 221 103 642.2 0 103 222 83 642.2 0 83 223 136 642.2 0 136 224 113 642.2 0 113 225 153 642.2 0 153 226 132 642.2 0 132 227 105 642.2 0 105 228 297 642.2 0 297 229 290 642.2 0 290 230 242 642.2 0 242 231 131 642.2 0 131 232 164 642.2 0 164 233 651 642.2 0 651 234 335 642.2 0 335 235 246 642.2 0 246 236 102 642.2 0 102 237 367 642.2 0 367 238 294 642.2 0 294 239 167 642.2 0 167 240 202 642.2 0 202 241 113 642.2 0 113 242 122 642.2 0 122 243 109 642.2 0 109 244 101 642.2 0 101 245 102 642.2 0 102 246 88 642.2 0 88 247 99 642.2 0 99 248 271 642.2 0 271 249 631 642.2 0 631 250 620 642.2 0 620 251 477 642.2 0 477 252 375 642.2 0 375 253 1154 642.2 0 1154 254 531 642.2 0 531 255 949 642.2 0 949 256 150 642.2 0 150 257 181 642.2 0 181 258 322 642.2 0 322 259 980 642.2 0 980 260 825 642.2 0 825 261 3771 642.2 0 3771 262 11041 642.2 0 11041 263 13005 642.2 0 13005 264 6579 642.2 0 6579 265 2503 642.2 0 2503 266 101 642.2 0 101 267 132 642.2 0 132 268 138 642.2 0 138 269 211 642.2 0 211 270 92 642.2 0 92 271 85 642.2 0 85 272 113 642.2 0 113 273 140 642.2 0 140 274 179 642.2 0 179 275 287 642.2 0 287 276 186 642.2 0 186 277 282 642.2 0 282 278 259 642.2 0 259 279 194 642.2 0 194 280 176 642.2 0 176 281 178 642.2 0 178 282 401 642.2 0 401 283 286 642.2 0 286 284 329 642.2 0 329 285 168 642.2 0 168 286 231 642.2 0 231 287 1057 642.2 0 1057 288 2012 642.2 0 2012 289 1719 642.2 0 1719 290 333 642.2 0 333 291 392 642.2 0 392 292 974 642.2 0 974 293 853 642.2 0 853 294 604 642.2 0 604 295 604 642.2 0 604 296 409 642.2 0 409 297 609 642.2 0 609 298 1750 642.2 0 1750 299 1981 642.2 0 1981 300 2394 642.2 0 2394 301 245 642.2 0 245 Finished in 3.58 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 78,400 Reads with adapters: 15,456 (19.7%) Reads that were too short: 61 (0.1%) Reads written (passing filters): 15,395 (19.6%) Total basepairs processed: 16,774,663 bp Total written (filtered): 4,213,010 bp (25.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15456 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 2 0 1 19 107 0.0 3 0 77 12 13 5 20 14939 0.0 4 13960 782 155 34 8 21 39 0.0 4 4 34 0 1 22 7 0.0 4 2 3 1 0 1 23 240 0.0 4 196 12 3 20 9 24 2 0.0 4 0 1 0 1 26 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 64 6 0.0 4 0 0 0 1 5 65 5 0.0 4 0 0 0 0 5 66 4 0.0 4 0 0 0 0 4 70 13 0.0 4 0 0 0 0 13 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 2 0.0 4 0 1 0 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 113 5 0.0 4 0 0 0 0 5 114 7 0.0 4 0 0 0 1 6 115 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 125 5 0.0 4 0 0 0 0 5 127 27 0.0 4 0 0 0 0 27 133 1 0.0 4 0 0 0 0 1 144 1 0.0 4 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 1 164 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 3 0.0 4 0 0 0 0 3 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 15,395 Reads with adapters: 1,508 (9.8%) Reads written (passing filters): 15,395 (100.0%) Total basepairs processed: 4,213,010 bp Total written (filtered): 4,196,128 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1508 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.4% C: 14.2% G: 78.0% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 83 240.5 0 83 4 6 60.1 0 6 9 19 0.1 0 2 17 10 62 0.0 1 5 57 11 1087 0.0 1 53 1034 12 236 0.0 1 11 225 13 7 0.0 1 0 7 14 2 0.0 1 0 2 15 1 0.0 1 0 1 152 2 0.0 1 1 1 171 1 0.0 1 1 210 2 0.0 1 2