This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.19 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 376,176 (14.3%) Reads that were too short: 12,727 (0.5%) Reads written (passing filters): 363,449 (13.8%) Total basepairs processed: 791,719,999 bp Total written (filtered): 67,912,454 bp (8.6%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 376176 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 208 642.2 0 208 7 167 642.2 0 167 8 2383 642.2 0 2383 9 2312 642.2 0 2312 10 104 642.2 0 104 11 103 642.2 0 103 12 92 642.2 0 92 13 87 642.2 0 87 14 115 642.2 0 115 15 114 642.2 0 114 16 116 642.2 0 116 17 130 642.2 0 130 18 123 642.2 0 123 19 109 642.2 0 109 20 90 642.2 0 90 21 99 642.2 0 99 22 120 642.2 0 120 23 104 642.2 0 104 24 138 642.2 0 138 25 276 642.2 0 276 26 390 642.2 0 390 27 322 642.2 0 322 28 89 642.2 0 89 29 102 642.2 0 102 30 99 642.2 0 99 31 131 642.2 0 131 32 144 642.2 0 144 33 117 642.2 0 117 34 94 642.2 0 94 35 108 642.2 0 108 36 118 642.2 0 118 37 209 642.2 0 209 38 143 642.2 0 143 39 125 642.2 0 125 40 119 642.2 0 119 41 85 642.2 0 85 42 94 642.2 0 94 43 135 642.2 0 135 44 105 642.2 0 105 45 96 642.2 0 96 46 91 642.2 0 91 47 125 642.2 0 125 48 86 642.2 0 86 49 87 642.2 0 87 50 65 642.2 0 65 51 95 642.2 0 95 52 82 642.2 0 82 53 97 642.2 0 97 54 78 642.2 0 78 55 114 642.2 0 114 56 94 642.2 0 94 57 94 642.2 0 94 58 91 642.2 0 91 59 84 642.2 0 84 60 115 642.2 0 115 61 75 642.2 0 75 62 94 642.2 0 94 63 109 642.2 0 109 64 107 642.2 0 107 65 88 642.2 0 88 66 124 642.2 0 124 67 90 642.2 0 90 68 101 642.2 0 101 69 178 642.2 0 178 70 596 642.2 0 596 71 688 642.2 0 688 72 1436 642.2 0 1436 73 95 642.2 0 95 74 116 642.2 0 116 75 88 642.2 0 88 76 106 642.2 0 106 77 122 642.2 0 122 78 135 642.2 0 135 79 91 642.2 0 91 80 99 642.2 0 99 81 98 642.2 0 98 82 121 642.2 0 121 83 161 642.2 0 161 84 335 642.2 0 335 85 110 642.2 0 110 86 327 642.2 0 327 87 97 642.2 0 97 88 88 642.2 0 88 89 84 642.2 0 84 90 90 642.2 0 90 91 92 642.2 0 92 92 99 642.2 0 99 93 132 642.2 0 132 94 96 642.2 0 96 95 222 642.2 0 222 96 417 642.2 0 417 97 150 642.2 0 150 98 232 642.2 0 232 99 190 642.2 0 190 100 175 642.2 0 175 101 207 642.2 0 207 102 151 642.2 0 151 103 179 642.2 0 179 104 139 642.2 0 139 105 187 642.2 0 187 106 324 642.2 0 324 107 405 642.2 0 405 108 763 642.2 0 763 109 1098 642.2 0 1098 110 1486 642.2 0 1486 111 2278 642.2 0 2278 112 14231 642.2 0 14231 113 133444 642.2 0 133444 114 82216 642.2 0 82216 115 48141 642.2 0 48141 116 325 642.2 0 325 117 119 642.2 0 119 118 154 642.2 0 154 119 120 642.2 0 120 120 100 642.2 0 100 121 134 642.2 0 134 122 179 642.2 0 179 123 126 642.2 0 126 124 152 642.2 0 152 125 212 642.2 0 212 126 156 642.2 0 156 127 382 642.2 0 382 128 295 642.2 0 295 129 247 642.2 0 247 130 295 642.2 0 295 131 252 642.2 0 252 132 332 642.2 0 332 133 526 642.2 0 526 134 1341 642.2 0 1341 135 32878 642.2 0 32878 136 4902 642.2 0 4902 137 5699 642.2 0 5699 138 343 642.2 0 343 139 267 642.2 0 267 140 267 642.2 0 267 141 742 642.2 0 742 142 140 642.2 0 140 143 236 642.2 0 236 144 166 642.2 0 166 145 974 642.2 0 974 146 115 642.2 0 115 147 115 642.2 0 115 148 229 642.2 0 229 149 117 642.2 0 117 150 190 642.2 0 190 151 90 642.2 0 90 152 104 642.2 0 104 153 86 642.2 0 86 154 95 642.2 0 95 155 99 642.2 0 99 156 114 642.2 0 114 157 122 642.2 0 122 158 201 642.2 0 201 159 400 642.2 0 400 160 214 642.2 0 214 161 151 642.2 0 151 162 81 642.2 0 81 163 102 642.2 0 102 164 254 642.2 0 254 165 105 642.2 0 105 166 151 642.2 0 151 167 96 642.2 0 96 168 118 642.2 0 118 169 87 642.2 0 87 170 126 642.2 0 126 171 92 642.2 0 92 172 63 642.2 0 63 173 88 642.2 0 88 174 86 642.2 0 86 175 104 642.2 0 104 176 120 642.2 0 120 177 99 642.2 0 99 178 96 642.2 0 96 179 126 642.2 0 126 180 105 642.2 0 105 181 135 642.2 0 135 182 145 642.2 0 145 183 141 642.2 0 141 184 107 642.2 0 107 185 148 642.2 0 148 186 108 642.2 0 108 187 96 642.2 0 96 188 175 642.2 0 175 189 96 642.2 0 96 190 109 642.2 0 109 191 93 642.2 0 93 192 126 642.2 0 126 193 99 642.2 0 99 194 82 642.2 0 82 195 107 642.2 0 107 196 121 642.2 0 121 197 96 642.2 0 96 198 103 642.2 0 103 199 151 642.2 0 151 200 121 642.2 0 121 201 172 642.2 0 172 202 173 642.2 0 173 203 219 642.2 0 219 204 171 642.2 0 171 205 133 642.2 0 133 206 238 642.2 0 238 207 141 642.2 0 141 208 158 642.2 0 158 209 94 642.2 0 94 210 96 642.2 0 96 211 101 642.2 0 101 212 108 642.2 0 108 213 105 642.2 0 105 214 131 642.2 0 131 215 125 642.2 0 125 216 145 642.2 0 145 217 98 642.2 0 98 218 83 642.2 0 83 219 130 642.2 0 130 220 117 642.2 0 117 221 152 642.2 0 152 222 88 642.2 0 88 223 126 642.2 0 126 224 334 642.2 0 334 225 375 642.2 0 375 226 364 642.2 0 364 227 80 642.2 0 80 228 79 642.2 0 79 229 88 642.2 0 88 230 80 642.2 0 80 231 72 642.2 0 72 232 83 642.2 0 83 233 103 642.2 0 103 234 200 642.2 0 200 235 236 642.2 0 236 236 223 642.2 0 223 237 85 642.2 0 85 238 77 642.2 0 77 239 75 642.2 0 75 240 81 642.2 0 81 241 95 642.2 0 95 242 100 642.2 0 100 243 84 642.2 0 84 244 113 642.2 0 113 245 118 642.2 0 118 246 120 642.2 0 120 247 78 642.2 0 78 248 94 642.2 0 94 249 102 642.2 0 102 250 103 642.2 0 103 251 979 642.2 0 979 252 89 642.2 0 89 253 75 642.2 0 75 254 70 642.2 0 70 255 111 642.2 0 111 256 101 642.2 0 101 257 115 642.2 0 115 258 81 642.2 0 81 259 103 642.2 0 103 260 80 642.2 0 80 261 89 642.2 0 89 262 68 642.2 0 68 263 71 642.2 0 71 264 93 642.2 0 93 265 76 642.2 0 76 266 67 642.2 0 67 267 79 642.2 0 79 268 110 642.2 0 110 269 112 642.2 0 112 270 98 642.2 0 98 271 110 642.2 0 110 272 173 642.2 0 173 273 161 642.2 0 161 274 211 642.2 0 211 275 149 642.2 0 149 276 100 642.2 0 100 277 83 642.2 0 83 278 62 642.2 0 62 279 81 642.2 0 81 280 132 642.2 0 132 281 111 642.2 0 111 282 170 642.2 0 170 283 99 642.2 0 99 284 85 642.2 0 85 285 85 642.2 0 85 286 81 642.2 0 81 287 109 642.2 0 109 288 93 642.2 0 93 289 93 642.2 0 93 290 77 642.2 0 77 291 103 642.2 0 103 292 88 642.2 0 88 293 176 642.2 0 176 294 154 642.2 0 154 295 195 642.2 0 195 296 115 642.2 0 115 297 108 642.2 0 108 298 76 642.2 0 76 299 68 642.2 0 68 300 66 642.2 0 66 301 72 642.2 0 72 Finished in 13.57 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 363,449 Reads with adapters: 2,341 (0.6%) Reads that were too short: 27 (0.0%) Reads written (passing filters): 2,314 (0.6%) Total basepairs processed: 67,912,454 bp Total written (filtered): 627,598 bp (0.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2341 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 9 0.0 3 0 5 3 1 20 2255 0.0 4 2182 63 7 2 1 21 19 0.0 4 5 12 1 1 22 4 0.0 4 0 0 2 2 23 1 0.0 4 0 0 0 1 24 3 0.0 4 0 0 0 0 3 26 1 0.0 4 1 38 2 0.0 4 0 0 0 0 2 40 1 0.0 4 0 0 1 55 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 78 3 0.0 4 1 1 1 79 2 0.0 4 1 0 0 0 1 80 6 0.0 4 4 2 81 1 0.0 4 1 82 1 0.0 4 1 85 2 0.0 4 0 0 0 0 2 86 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 101 1 0.0 4 1 121 1 0.0 4 0 0 0 0 1 122 4 0.0 4 0 0 0 0 4 123 4 0.0 4 0 0 0 0 4 126 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 157 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 1 166 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.07 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 2,314 Reads with adapters: 2,021 (87.3%) Reads written (passing filters): 2,314 (100.0%) Total basepairs processed: 627,598 bp Total written (filtered): 105,654 bp (16.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2021 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.1% C: 0.4% G: 2.8% T: 96.7% none/other: 0.0% WARNING: The adapter is preceded by "T" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "T" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 9.0 0 1 5 1 2.3 0 1 9 5 0.0 0 1 4 10 58 0.0 1 4 54 265 6 0.0 1 6 266 1000 0.0 1 934 66 267 904 0.0 1 847 57 268 28 0.0 1 27 1 269 12 0.0 1 12 270 6 0.0 1 5 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.