This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATCTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.52 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 407,683 (15.5%) Reads that were too short: 8,814 (0.3%) Reads written (passing filters): 398,869 (15.2%) Total basepairs processed: 791,719,999 bp Total written (filtered): 74,578,678 bp (9.4%) === Adapter 1 === Sequence: TATCTG; Type: regular 5'; Length: 6; Trimmed: 407683 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 138 642.2 0 138 7 153 642.2 0 153 8 2410 642.2 0 2410 9 2404 642.2 0 2404 10 80 642.2 0 80 11 84 642.2 0 84 12 89 642.2 0 89 13 85 642.2 0 85 14 110 642.2 0 110 15 106 642.2 0 106 16 82 642.2 0 82 17 125 642.2 0 125 18 122 642.2 0 122 19 96 642.2 0 96 20 79 642.2 0 79 21 96 642.2 0 96 22 94 642.2 0 94 23 99 642.2 0 99 24 116 642.2 0 116 25 165 642.2 0 165 26 176 642.2 0 176 27 128 642.2 0 128 28 98 642.2 0 98 29 88 642.2 0 88 30 120 642.2 0 120 31 108 642.2 0 108 32 138 642.2 0 138 33 109 642.2 0 109 34 100 642.2 0 100 35 79 642.2 0 79 36 89 642.2 0 89 37 120 642.2 0 120 38 114 642.2 0 114 39 86 642.2 0 86 40 90 642.2 0 90 41 84 642.2 0 84 42 87 642.2 0 87 43 168 642.2 0 168 44 74 642.2 0 74 45 74 642.2 0 74 46 81 642.2 0 81 47 71 642.2 0 71 48 64 642.2 0 64 49 81 642.2 0 81 50 51 642.2 0 51 51 66 642.2 0 66 52 76 642.2 0 76 53 90 642.2 0 90 54 66 642.2 0 66 55 127 642.2 0 127 56 108 642.2 0 108 57 95 642.2 0 95 58 86 642.2 0 86 59 76 642.2 0 76 60 77 642.2 0 77 61 73 642.2 0 73 62 65 642.2 0 65 63 107 642.2 0 107 64 78 642.2 0 78 65 73 642.2 0 73 66 80 642.2 0 80 67 81 642.2 0 81 68 105 642.2 0 105 69 171 642.2 0 171 70 659 642.2 0 659 71 746 642.2 0 746 72 1502 642.2 0 1502 73 99 642.2 0 99 74 95 642.2 0 95 75 80 642.2 0 80 76 138 642.2 0 138 77 113 642.2 0 113 78 109 642.2 0 109 79 90 642.2 0 90 80 75 642.2 0 75 81 90 642.2 0 90 82 95 642.2 0 95 83 143 642.2 0 143 84 300 642.2 0 300 85 134 642.2 0 134 86 433 642.2 0 433 87 89 642.2 0 89 88 92 642.2 0 92 89 95 642.2 0 95 90 83 642.2 0 83 91 71 642.2 0 71 92 112 642.2 0 112 93 142 642.2 0 142 94 113 642.2 0 113 95 203 642.2 0 203 96 528 642.2 0 528 97 152 642.2 0 152 98 224 642.2 0 224 99 186 642.2 0 186 100 187 642.2 0 187 101 296 642.2 0 296 102 153 642.2 0 153 103 162 642.2 0 162 104 151 642.2 0 151 105 194 642.2 0 194 106 277 642.2 0 277 107 447 642.2 0 447 108 770 642.2 0 770 109 1095 642.2 0 1095 110 1609 642.2 0 1609 111 2239 642.2 0 2239 112 12079 642.2 0 12079 113 146919 642.2 0 146919 114 98136 642.2 0 98136 115 59659 642.2 0 59659 116 331 642.2 0 331 117 113 642.2 0 113 118 156 642.2 0 156 119 110 642.2 0 110 120 90 642.2 0 90 121 110 642.2 0 110 122 166 642.2 0 166 123 121 642.2 0 121 124 130 642.2 0 130 125 216 642.2 0 216 126 113 642.2 0 113 127 379 642.2 0 379 128 263 642.2 0 263 129 227 642.2 0 227 130 211 642.2 0 211 131 218 642.2 0 218 132 319 642.2 0 319 133 471 642.2 0 471 134 1030 642.2 0 1030 135 33063 642.2 0 33063 136 5081 642.2 0 5081 137 5918 642.2 0 5918 138 240 642.2 0 240 139 201 642.2 0 201 140 179 642.2 0 179 141 815 642.2 0 815 142 128 642.2 0 128 143 223 642.2 0 223 144 145 642.2 0 145 145 1003 642.2 0 1003 146 94 642.2 0 94 147 85 642.2 0 85 148 156 642.2 0 156 149 83 642.2 0 83 150 126 642.2 0 126 151 69 642.2 0 69 152 74 642.2 0 74 153 81 642.2 0 81 154 83 642.2 0 83 155 66 642.2 0 66 156 87 642.2 0 87 157 75 642.2 0 75 158 117 642.2 0 117 159 183 642.2 0 183 160 113 642.2 0 113 161 88 642.2 0 88 162 86 642.2 0 86 163 87 642.2 0 87 164 100 642.2 0 100 165 83 642.2 0 83 166 78 642.2 0 78 167 82 642.2 0 82 168 75 642.2 0 75 169 79 642.2 0 79 170 100 642.2 0 100 171 87 642.2 0 87 172 50 642.2 0 50 173 68 642.2 0 68 174 59 642.2 0 59 175 83 642.2 0 83 176 77 642.2 0 77 177 73 642.2 0 73 178 59 642.2 0 59 179 74 642.2 0 74 180 82 642.2 0 82 181 138 642.2 0 138 182 125 642.2 0 125 183 88 642.2 0 88 184 61 642.2 0 61 185 85 642.2 0 85 186 72 642.2 0 72 187 74 642.2 0 74 188 110 642.2 0 110 189 70 642.2 0 70 190 102 642.2 0 102 191 73 642.2 0 73 192 95 642.2 0 95 193 84 642.2 0 84 194 65 642.2 0 65 195 47 642.2 0 47 196 83 642.2 0 83 197 62 642.2 0 62 198 84 642.2 0 84 199 70 642.2 0 70 200 74 642.2 0 74 201 100 642.2 0 100 202 90 642.2 0 90 203 162 642.2 0 162 204 91 642.2 0 91 205 77 642.2 0 77 206 102 642.2 0 102 207 79 642.2 0 79 208 101 642.2 0 101 209 64 642.2 0 64 210 62 642.2 0 62 211 72 642.2 0 72 212 51 642.2 0 51 213 64 642.2 0 64 214 44 642.2 0 44 215 56 642.2 0 56 216 61 642.2 0 61 217 60 642.2 0 60 218 47 642.2 0 47 219 55 642.2 0 55 220 77 642.2 0 77 221 101 642.2 0 101 222 71 642.2 0 71 223 71 642.2 0 71 224 317 642.2 0 317 225 214 642.2 0 214 226 172 642.2 0 172 227 62 642.2 0 62 228 65 642.2 0 65 229 73 642.2 0 73 230 59 642.2 0 59 231 52 642.2 0 52 232 56 642.2 0 56 233 52 642.2 0 52 234 83 642.2 0 83 235 102 642.2 0 102 236 84 642.2 0 84 237 78 642.2 0 78 238 45 642.2 0 45 239 90 642.2 0 90 240 58 642.2 0 58 241 93 642.2 0 93 242 80 642.2 0 80 243 75 642.2 0 75 244 101 642.2 0 101 245 82 642.2 0 82 246 70 642.2 0 70 247 70 642.2 0 70 248 70 642.2 0 70 249 90 642.2 0 90 250 92 642.2 0 92 251 1067 642.2 0 1067 252 75 642.2 0 75 253 58 642.2 0 58 254 61 642.2 0 61 255 79 642.2 0 79 256 70 642.2 0 70 257 83 642.2 0 83 258 75 642.2 0 75 259 74 642.2 0 74 260 71 642.2 0 71 261 66 642.2 0 66 262 59 642.2 0 59 263 48 642.2 0 48 264 65 642.2 0 65 265 57 642.2 0 57 266 54 642.2 0 54 267 68 642.2 0 68 268 67 642.2 0 67 269 52 642.2 0 52 270 59 642.2 0 59 271 107 642.2 0 107 272 152 642.2 0 152 273 115 642.2 0 115 274 125 642.2 0 125 275 85 642.2 0 85 276 68 642.2 0 68 277 52 642.2 0 52 278 73 642.2 0 73 279 67 642.2 0 67 280 105 642.2 0 105 281 88 642.2 0 88 282 123 642.2 0 123 283 75 642.2 0 75 284 72 642.2 0 72 285 66 642.2 0 66 286 60 642.2 0 60 287 60 642.2 0 60 288 52 642.2 0 52 289 58 642.2 0 58 290 57 642.2 0 57 291 76 642.2 0 76 292 66 642.2 0 66 293 99 642.2 0 99 294 79 642.2 0 79 295 83 642.2 0 83 296 57 642.2 0 57 297 69 642.2 0 69 298 56 642.2 0 56 299 48 642.2 0 48 300 38 642.2 0 38 301 32 642.2 0 32 Finished in 13.31 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 398,869 Reads with adapters: 2,852 (0.7%) Reads that were too short: 435 (0.1%) Reads written (passing filters): 2,417 (0.6%) Total basepairs processed: 74,578,678 bp Total written (filtered): 658,422 bp (0.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2852 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 7 0.0 3 0 7 17 2342 0.0 3 2310 32 18 6 0.0 3 1 5 21 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 33 7 0.0 3 0 0 0 7 34 1 0.0 3 0 0 0 1 36 5 0.0 3 0 0 0 5 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 5 0.0 3 0 0 0 5 54 6 0.0 3 0 0 0 6 55 3 0.0 3 0 0 1 2 57 2 0.0 3 0 0 0 2 58 4 0.0 3 0 0 0 4 59 1 0.0 3 1 64 1 0.0 3 0 0 0 1 76 4 0.0 3 3 0 0 1 77 2 0.0 3 1 0 0 1 78 2 0.0 3 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 99 22 0.0 3 0 0 0 22 100 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 316 0.0 3 0 0 7 309 112 3 0.0 3 0 0 0 3 115 8 0.0 3 0 0 0 8 116 3 0.0 3 0 0 0 3 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 173 11 0.0 3 0 0 0 11 174 11 0.0 3 0 0 0 11 175 14 0.0 3 0 0 0 14 176 19 0.0 3 0 0 1 18 177 3 0.0 3 0 0 0 3 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 241 2 0.0 3 0 0 0 2 264 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.08 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 2,417 Reads with adapters: 56 (2.3%) Reads written (passing filters): 2,417 (100.0%) Total basepairs processed: 658,422 bp Total written (filtered): 658,244 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 56 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.9% C: 28.6% G: 19.6% T: 17.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 49 37.8 0 49 4 5 9.4 0 5 5 1 2.4 0 1 6 1 0.6 0 1