This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.75 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 742,553 (28.2%) Reads that were too short: 62,664 (2.4%) Reads written (passing filters): 679,889 (25.8%) Total basepairs processed: 791,719,999 bp Total written (filtered): 174,259,709 bp (22.0%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 742553 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 19647 642.2 0 19647 7 169341 642.2 0 169341 8 3852 642.2 0 3852 9 202078 642.2 0 202078 10 285 642.2 0 285 11 92 642.2 0 92 12 84 642.2 0 84 13 77 642.2 0 77 14 85 642.2 0 85 15 134 642.2 0 134 16 107 642.2 0 107 17 82 642.2 0 82 18 71 642.2 0 71 19 101 642.2 0 101 20 100 642.2 0 100 21 80 642.2 0 80 22 111 642.2 0 111 23 90 642.2 0 90 24 76 642.2 0 76 25 87 642.2 0 87 26 71 642.2 0 71 27 75 642.2 0 75 28 80 642.2 0 80 29 78 642.2 0 78 30 80 642.2 0 80 31 102 642.2 0 102 32 80 642.2 0 80 33 103 642.2 0 103 34 145 642.2 0 145 35 128 642.2 0 128 36 142 642.2 0 142 37 75 642.2 0 75 38 95 642.2 0 95 39 71 642.2 0 71 40 88 642.2 0 88 41 172 642.2 0 172 42 158 642.2 0 158 43 118 642.2 0 118 44 113 642.2 0 113 45 117 642.2 0 117 46 110 642.2 0 110 47 126 642.2 0 126 48 86 642.2 0 86 49 260 642.2 0 260 50 422 642.2 0 422 51 371 642.2 0 371 52 117 642.2 0 117 53 142 642.2 0 142 54 131 642.2 0 131 55 113 642.2 0 113 56 91 642.2 0 91 57 97 642.2 0 97 58 137 642.2 0 137 59 225 642.2 0 225 60 1864 642.2 0 1864 61 1854 642.2 0 1854 62 1542 642.2 0 1542 63 133 642.2 0 133 64 104 642.2 0 104 65 153 642.2 0 153 66 135 642.2 0 135 67 200 642.2 0 200 68 402 642.2 0 402 69 331 642.2 0 331 70 404 642.2 0 404 71 541 642.2 0 541 72 1302 642.2 0 1302 73 474 642.2 0 474 74 401 642.2 0 401 75 675 642.2 0 675 76 294 642.2 0 294 77 364 642.2 0 364 78 625 642.2 0 625 79 593 642.2 0 593 80 935 642.2 0 935 81 1197 642.2 0 1197 82 1632 642.2 0 1632 83 1946 642.2 0 1946 84 4262 642.2 0 4262 85 103119 642.2 0 103119 86 42551 642.2 0 42551 87 67548 642.2 0 67548 88 403 642.2 0 403 89 84 642.2 0 84 90 74 642.2 0 74 91 108 642.2 0 108 92 76 642.2 0 76 93 87 642.2 0 87 94 98 642.2 0 98 95 94 642.2 0 94 96 97 642.2 0 97 97 98 642.2 0 98 98 91 642.2 0 91 99 70 642.2 0 70 100 65 642.2 0 65 101 82 642.2 0 82 102 78 642.2 0 78 103 84 642.2 0 84 104 78 642.2 0 78 105 62 642.2 0 62 106 67 642.2 0 67 107 99 642.2 0 99 108 88 642.2 0 88 109 137 642.2 0 137 110 61 642.2 0 61 111 71 642.2 0 71 112 73 642.2 0 73 113 88 642.2 0 88 114 84 642.2 0 84 115 85 642.2 0 85 116 83 642.2 0 83 117 88 642.2 0 88 118 84 642.2 0 84 119 92 642.2 0 92 120 76 642.2 0 76 121 119 642.2 0 119 122 200 642.2 0 200 123 176 642.2 0 176 124 234 642.2 0 234 125 91 642.2 0 91 126 139 642.2 0 139 127 86 642.2 0 86 128 180 642.2 0 180 129 154 642.2 0 154 130 868 642.2 0 868 131 260 642.2 0 260 132 289 642.2 0 289 133 272 642.2 0 272 134 179 642.2 0 179 135 241 642.2 0 241 136 179 642.2 0 179 137 136 642.2 0 136 138 79 642.2 0 79 139 118 642.2 0 118 140 109 642.2 0 109 141 119 642.2 0 119 142 166 642.2 0 166 143 120 642.2 0 120 144 158 642.2 0 158 145 108 642.2 0 108 146 142 642.2 0 142 147 135 642.2 0 135 148 151 642.2 0 151 149 345 642.2 0 345 150 305 642.2 0 305 151 389 642.2 0 389 152 549 642.2 0 549 153 1866 642.2 0 1866 154 1821 642.2 0 1821 155 760 642.2 0 760 156 228 642.2 0 228 157 116 642.2 0 116 158 95 642.2 0 95 159 94 642.2 0 94 160 116 642.2 0 116 161 140 642.2 0 140 162 174 642.2 0 174 163 92 642.2 0 92 164 121 642.2 0 121 165 208 642.2 0 208 166 129 642.2 0 129 167 125 642.2 0 125 168 199 642.2 0 199 169 212 642.2 0 212 170 220 642.2 0 220 171 203 642.2 0 203 172 261 642.2 0 261 173 288 642.2 0 288 174 724 642.2 0 724 175 6399 642.2 0 6399 176 8875 642.2 0 8875 177 5276 642.2 0 5276 178 113 642.2 0 113 179 89 642.2 0 89 180 124 642.2 0 124 181 260 642.2 0 260 182 257 642.2 0 257 183 241 642.2 0 241 184 191 642.2 0 191 185 378 642.2 0 378 186 161 642.2 0 161 187 138 642.2 0 138 188 133 642.2 0 133 189 814 642.2 0 814 190 192 642.2 0 192 191 187 642.2 0 187 192 133 642.2 0 133 193 111 642.2 0 111 194 135 642.2 0 135 195 159 642.2 0 159 196 138 642.2 0 138 197 117 642.2 0 117 198 132 642.2 0 132 199 102 642.2 0 102 200 121 642.2 0 121 201 140 642.2 0 140 202 94 642.2 0 94 203 132 642.2 0 132 204 277 642.2 0 277 205 299 642.2 0 299 206 458 642.2 0 458 207 198 642.2 0 198 208 447 642.2 0 447 209 257 642.2 0 257 210 365 642.2 0 365 211 602 642.2 0 602 212 648 642.2 0 648 213 6161 642.2 0 6161 214 20741 642.2 0 20741 215 6474 642.2 0 6474 216 194 642.2 0 194 217 348 642.2 0 348 218 337 642.2 0 337 219 186 642.2 0 186 220 194 642.2 0 194 221 181 642.2 0 181 222 174 642.2 0 174 223 96 642.2 0 96 224 115 642.2 0 115 225 120 642.2 0 120 226 137 642.2 0 137 227 187 642.2 0 187 228 300 642.2 0 300 229 232 642.2 0 232 230 274 642.2 0 274 231 406 642.2 0 406 232 433 642.2 0 433 233 317 642.2 0 317 234 170 642.2 0 170 235 205 642.2 0 205 236 164 642.2 0 164 237 150 642.2 0 150 238 139 642.2 0 139 239 125 642.2 0 125 240 140 642.2 0 140 241 138 642.2 0 138 242 98 642.2 0 98 243 153 642.2 0 153 244 333 642.2 0 333 245 129 642.2 0 129 246 188 642.2 0 188 247 217 642.2 0 217 248 259 642.2 0 259 249 319 642.2 0 319 250 404 642.2 0 404 251 231 642.2 0 231 252 207 642.2 0 207 253 918 642.2 0 918 254 1129 642.2 0 1129 255 1020 642.2 0 1020 256 433 642.2 0 433 257 373 642.2 0 373 258 331 642.2 0 331 259 187 642.2 0 187 260 132 642.2 0 132 261 155 642.2 0 155 262 145 642.2 0 145 263 94 642.2 0 94 264 85 642.2 0 85 265 167 642.2 0 167 266 158 642.2 0 158 267 211 642.2 0 211 268 149 642.2 0 149 269 167 642.2 0 167 270 171 642.2 0 171 271 185 642.2 0 185 272 153 642.2 0 153 273 132 642.2 0 132 274 215 642.2 0 215 275 170 642.2 0 170 276 298 642.2 0 298 277 197 642.2 0 197 278 447 642.2 0 447 279 256 642.2 0 256 280 273 642.2 0 273 281 280 642.2 0 280 282 175 642.2 0 175 283 153 642.2 0 153 284 178 642.2 0 178 285 566 642.2 0 566 286 666 642.2 0 666 287 559 642.2 0 559 288 329 642.2 0 329 289 196 642.2 0 196 290 233 642.2 0 233 291 178 642.2 0 178 292 215 642.2 0 215 293 383 642.2 0 383 294 398 642.2 0 398 295 428 642.2 0 428 296 495 642.2 0 495 297 805 642.2 0 805 298 1029 642.2 0 1029 299 806 642.2 0 806 300 645 642.2 0 645 301 443 642.2 0 443 Finished in 30.76 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 679,889 Reads with adapters: 186,178 (27.4%) Reads that were too short: 392 (0.1%) Reads written (passing filters): 185,786 (27.3%) Total basepairs processed: 174,259,709 bp Total written (filtered): 50,827,996 bp (29.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 186178 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 4 6 18 54 0.0 3 0 0 31 9 14 19 1120 0.0 3 0 784 172 113 51 20 182923 0.0 4 170349 9957 1911 540 166 21 387 0.0 4 41 309 21 7 9 22 39 0.0 4 2 0 27 3 7 23 393 0.0 4 3 1 2 263 124 24 6 0.0 4 1 0 0 3 2 25 2 0.0 4 0 1 0 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 4 0.0 4 0 0 0 0 4 29 1 0.0 4 0 0 0 0 1 30 3 0.0 4 0 1 0 0 2 31 2 0.0 4 0 1 0 0 1 32 1 0.0 4 1 36 1 0.0 4 1 37 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 41 2 0.0 4 1 0 0 0 1 42 2 0.0 4 0 0 0 0 2 44 2 0.0 4 0 0 0 0 2 45 2 0.0 4 0 0 0 0 2 46 2 0.0 4 0 0 0 0 2 49 92 0.0 4 0 0 0 0 92 50 2 0.0 4 0 0 0 0 2 51 2 0.0 4 0 0 0 0 2 52 2 0.0 4 0 0 1 0 1 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 1 57 4 0.0 4 0 0 0 0 4 59 3 0.0 4 0 0 0 0 3 61 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 67 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 76 4 0.0 4 0 0 0 1 3 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 84 1 0.0 4 1 87 2 0.0 4 0 0 0 0 2 88 4 0.0 4 0 0 0 0 4 89 7 0.0 4 0 0 0 0 7 90 7 0.0 4 0 0 0 0 7 91 14 0.0 4 0 0 0 0 14 92 3 0.0 4 0 0 0 0 3 94 2 0.0 4 0 0 0 0 2 95 1 0.0 4 0 0 0 0 1 100 5 0.0 4 0 0 0 0 5 102 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 2 0.0 4 0 1 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 1 119 1 0.0 4 1 124 3 0.0 4 0 1 0 0 2 125 5 0.0 4 0 0 0 0 5 126 8 0.0 4 0 0 0 0 8 127 637 0.0 4 0 0 0 0 637 128 6 0.0 4 0 0 0 0 6 129 7 0.0 4 0 0 0 0 7 130 3 0.0 4 0 0 0 0 3 131 1 0.0 4 0 0 0 0 1 133 3 0.0 4 0 0 0 0 3 135 5 0.0 4 0 0 0 0 5 136 6 0.0 4 0 0 0 0 6 137 17 0.0 4 0 0 0 0 17 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 2 0.0 4 0 0 0 0 2 141 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 146 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 33 0.0 4 0 0 0 3 30 151 18 0.0 4 0 0 0 2 16 152 3 0.0 4 0 0 0 0 3 153 26 0.0 4 0 0 0 1 25 155 3 0.0 4 0 0 0 0 3 156 2 0.0 4 0 0 0 0 2 158 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 181 3 0.0 4 0 0 0 1 2 183 3 0.0 4 0 0 0 0 3 184 27 0.0 4 0 0 0 0 27 185 13 0.0 4 0 0 0 0 13 186 14 0.0 4 0 0 0 0 14 187 3 0.0 4 0 0 0 0 3 191 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 1 202 1 0.0 4 0 0 0 0 1 204 2 0.0 4 0 0 0 0 2 205 3 0.0 4 0 0 0 0 3 206 1 0.0 4 0 0 0 0 1 207 4 0.0 4 0 0 0 1 3 208 4 0.0 4 0 0 0 0 4 209 2 0.0 4 0 0 0 0 2 213 3 0.0 4 0 0 0 0 3 214 5 0.0 4 0 0 0 0 5 215 13 0.0 4 0 0 0 0 13 216 6 0.0 4 0 0 0 0 6 220 1 0.0 4 0 0 0 0 1 223 2 0.0 4 0 0 0 1 1 224 7 0.0 4 0 0 0 0 7 225 5 0.0 4 0 0 0 0 5 226 3 0.0 4 0 0 0 0 3 227 1 0.0 4 0 0 0 0 1 228 14 0.0 4 0 0 0 0 14 229 7 0.0 4 0 0 0 0 7 230 1 0.0 4 0 0 0 0 1 233 6 0.0 4 0 0 0 0 6 234 6 0.0 4 0 0 0 0 6 235 3 0.0 4 0 0 0 0 3 236 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 254 2 0.0 4 0 0 0 0 2 257 5 0.0 4 0 0 0 0 5 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 20 0.0 4 0 0 0 2 18 263 1 0.0 4 0 0 0 0 1 264 12 0.0 4 0 0 0 1 11 265 2 0.0 4 0 0 0 0 2 268 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 3 0.0 4 0 0 0 0 3 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.43 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 185,786 Reads with adapters: 10,242 (5.5%) Reads written (passing filters): 185,786 (100.0%) Total basepairs processed: 50,827,996 bp Total written (filtered): 50,698,674 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10242 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.4% C: 16.2% G: 71.9% T: 2.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1033 2902.9 0 1033 4 170 725.7 0 170 5 11 181.4 0 11 6 6 45.4 0 6 7 2 11.3 0 2 8 3 2.8 0 3 9 62 0.7 0 7 55 10 197 0.2 1 5 192 11 4248 0.0 1 240 4008 12 4142 0.0 1 127 4015 13 220 0.0 1 14 206 14 13 0.0 1 1 12 15 15 0.0 1 1 14 16 1 0.0 1 0 1 20 1 0.0 1 0 1 35 1 0.0 1 1 42 1 0.0 1 0 1 57 1 0.0 1 1 60 1 0.0 1 1 72 1 0.0 1 1 93 1 0.0 1 0 1 109 1 0.0 1 1 142 1 0.0 1 1 144 2 0.0 1 2 152 10 0.0 1 7 3 153 3 0.0 1 2 1 156 1 0.0 1 1 162 1 0.0 1 0 1 171 1 0.0 1 1 191 1 0.0 1 0 1 192 1 0.0 1 0 1 208 2 0.0 1 2 210 69 0.0 1 55 14 211 7 0.0 1 5 2 230 3 0.0 1 2 1 257 8 0.0 1 8 273 1 0.0 1 1