This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.52 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 758,796 (28.8%) Reads that were too short: 65,167 (2.5%) Reads written (passing filters): 693,629 (26.4%) Total basepairs processed: 791,719,999 bp Total written (filtered): 178,928,386 bp (22.6%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 758796 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 21934 642.2 0 21934 7 199702 642.2 0 199702 8 2499 642.2 0 2499 9 194968 642.2 0 194968 10 241 642.2 0 241 11 91 642.2 0 91 12 96 642.2 0 96 13 80 642.2 0 80 14 61 642.2 0 61 15 92 642.2 0 92 16 106 642.2 0 106 17 96 642.2 0 96 18 104 642.2 0 104 19 123 642.2 0 123 20 121 642.2 0 121 21 104 642.2 0 104 22 104 642.2 0 104 23 91 642.2 0 91 24 123 642.2 0 123 25 80 642.2 0 80 26 93 642.2 0 93 27 104 642.2 0 104 28 106 642.2 0 106 29 109 642.2 0 109 30 98 642.2 0 98 31 89 642.2 0 89 32 101 642.2 0 101 33 111 642.2 0 111 34 121 642.2 0 121 35 149 642.2 0 149 36 129 642.2 0 129 37 91 642.2 0 91 38 85 642.2 0 85 39 86 642.2 0 86 40 100 642.2 0 100 41 188 642.2 0 188 42 157 642.2 0 157 43 136 642.2 0 136 44 119 642.2 0 119 45 139 642.2 0 139 46 118 642.2 0 118 47 126 642.2 0 126 48 83 642.2 0 83 49 130 642.2 0 130 50 170 642.2 0 170 51 147 642.2 0 147 52 130 642.2 0 130 53 227 642.2 0 227 54 93 642.2 0 93 55 118 642.2 0 118 56 106 642.2 0 106 57 132 642.2 0 132 58 126 642.2 0 126 59 172 642.2 0 172 60 511 642.2 0 511 61 695 642.2 0 695 62 401 642.2 0 401 63 153 642.2 0 153 64 99 642.2 0 99 65 183 642.2 0 183 66 165 642.2 0 165 67 211 642.2 0 211 68 232 642.2 0 232 69 274 642.2 0 274 70 325 642.2 0 325 71 493 642.2 0 493 72 1267 642.2 0 1267 73 450 642.2 0 450 74 400 642.2 0 400 75 626 642.2 0 626 76 345 642.2 0 345 77 384 642.2 0 384 78 677 642.2 0 677 79 634 642.2 0 634 80 856 642.2 0 856 81 1061 642.2 0 1061 82 1516 642.2 0 1516 83 1735 642.2 0 1735 84 3161 642.2 0 3161 85 100080 642.2 0 100080 86 38740 642.2 0 38740 87 69090 642.2 0 69090 88 370 642.2 0 370 89 81 642.2 0 81 90 153 642.2 0 153 91 105 642.2 0 105 92 86 642.2 0 86 93 114 642.2 0 114 94 112 642.2 0 112 95 115 642.2 0 115 96 128 642.2 0 128 97 127 642.2 0 127 98 130 642.2 0 130 99 111 642.2 0 111 100 102 642.2 0 102 101 90 642.2 0 90 102 124 642.2 0 124 103 121 642.2 0 121 104 92 642.2 0 92 105 83 642.2 0 83 106 90 642.2 0 90 107 130 642.2 0 130 108 90 642.2 0 90 109 190 642.2 0 190 110 100 642.2 0 100 111 113 642.2 0 113 112 86 642.2 0 86 113 101 642.2 0 101 114 109 642.2 0 109 115 74 642.2 0 74 116 101 642.2 0 101 117 91 642.2 0 91 118 98 642.2 0 98 119 97 642.2 0 97 120 80 642.2 0 80 121 125 642.2 0 125 122 126 642.2 0 126 123 137 642.2 0 137 124 107 642.2 0 107 125 101 642.2 0 101 126 100 642.2 0 100 127 88 642.2 0 88 128 130 642.2 0 130 129 131 642.2 0 131 130 228 642.2 0 228 131 185 642.2 0 185 132 155 642.2 0 155 133 146 642.2 0 146 134 103 642.2 0 103 135 134 642.2 0 134 136 149 642.2 0 149 137 128 642.2 0 128 138 137 642.2 0 137 139 132 642.2 0 132 140 107 642.2 0 107 141 123 642.2 0 123 142 134 642.2 0 134 143 116 642.2 0 116 144 158 642.2 0 158 145 109 642.2 0 109 146 117 642.2 0 117 147 119 642.2 0 119 148 125 642.2 0 125 149 316 642.2 0 316 150 260 642.2 0 260 151 395 642.2 0 395 152 413 642.2 0 413 153 1413 642.2 0 1413 154 1530 642.2 0 1530 155 294 642.2 0 294 156 165 642.2 0 165 157 144 642.2 0 144 158 123 642.2 0 123 159 85 642.2 0 85 160 134 642.2 0 134 161 155 642.2 0 155 162 231 642.2 0 231 163 131 642.2 0 131 164 174 642.2 0 174 165 220 642.2 0 220 166 109 642.2 0 109 167 118 642.2 0 118 168 212 642.2 0 212 169 161 642.2 0 161 170 195 642.2 0 195 171 229 642.2 0 229 172 287 642.2 0 287 173 301 642.2 0 301 174 550 642.2 0 550 175 6963 642.2 0 6963 176 10672 642.2 0 10672 177 6428 642.2 0 6428 178 151 642.2 0 151 179 113 642.2 0 113 180 122 642.2 0 122 181 183 642.2 0 183 182 145 642.2 0 145 183 140 642.2 0 140 184 178 642.2 0 178 185 215 642.2 0 215 186 149 642.2 0 149 187 138 642.2 0 138 188 105 642.2 0 105 189 964 642.2 0 964 190 118 642.2 0 118 191 174 642.2 0 174 192 85 642.2 0 85 193 94 642.2 0 94 194 110 642.2 0 110 195 107 642.2 0 107 196 137 642.2 0 137 197 143 642.2 0 143 198 109 642.2 0 109 199 112 642.2 0 112 200 112 642.2 0 112 201 104 642.2 0 104 202 81 642.2 0 81 203 104 642.2 0 104 204 131 642.2 0 131 205 168 642.2 0 168 206 198 642.2 0 198 207 183 642.2 0 183 208 556 642.2 0 556 209 262 642.2 0 262 210 380 642.2 0 380 211 699 642.2 0 699 212 627 642.2 0 627 213 8390 642.2 0 8390 214 32221 642.2 0 32221 215 9864 642.2 0 9864 216 123 642.2 0 123 217 158 642.2 0 158 218 191 642.2 0 191 219 115 642.2 0 115 220 111 642.2 0 111 221 130 642.2 0 130 222 144 642.2 0 144 223 64 642.2 0 64 224 92 642.2 0 92 225 90 642.2 0 90 226 82 642.2 0 82 227 91 642.2 0 91 228 134 642.2 0 134 229 106 642.2 0 106 230 133 642.2 0 133 231 316 642.2 0 316 232 200 642.2 0 200 233 186 642.2 0 186 234 97 642.2 0 97 235 84 642.2 0 84 236 110 642.2 0 110 237 112 642.2 0 112 238 88 642.2 0 88 239 83 642.2 0 83 240 121 642.2 0 121 241 108 642.2 0 108 242 102 642.2 0 102 243 116 642.2 0 116 244 325 642.2 0 325 245 110 642.2 0 110 246 124 642.2 0 124 247 117 642.2 0 117 248 107 642.2 0 107 249 133 642.2 0 133 250 131 642.2 0 131 251 109 642.2 0 109 252 105 642.2 0 105 253 304 642.2 0 304 254 354 642.2 0 354 255 266 642.2 0 266 256 141 642.2 0 141 257 198 642.2 0 198 258 142 642.2 0 142 259 112 642.2 0 112 260 71 642.2 0 71 261 110 642.2 0 110 262 67 642.2 0 67 263 59 642.2 0 59 264 69 642.2 0 69 265 131 642.2 0 131 266 78 642.2 0 78 267 76 642.2 0 76 268 105 642.2 0 105 269 71 642.2 0 71 270 92 642.2 0 92 271 89 642.2 0 89 272 84 642.2 0 84 273 70 642.2 0 70 274 114 642.2 0 114 275 83 642.2 0 83 276 114 642.2 0 114 277 70 642.2 0 70 278 124 642.2 0 124 279 120 642.2 0 120 280 105 642.2 0 105 281 116 642.2 0 116 282 90 642.2 0 90 283 94 642.2 0 94 284 89 642.2 0 89 285 185 642.2 0 185 286 228 642.2 0 228 287 240 642.2 0 240 288 109 642.2 0 109 289 88 642.2 0 88 290 145 642.2 0 145 291 104 642.2 0 104 292 79 642.2 0 79 293 139 642.2 0 139 294 128 642.2 0 128 295 113 642.2 0 113 296 164 642.2 0 164 297 206 642.2 0 206 298 312 642.2 0 312 299 182 642.2 0 182 300 193 642.2 0 193 301 132 642.2 0 132 Finished in 27.34 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 693,629 Reads with adapters: 202,340 (29.2%) Reads that were too short: 627 (0.1%) Reads written (passing filters): 201,713 (29.1%) Total basepairs processed: 178,928,386 bp Total written (filtered): 55,213,485 bp (30.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 202340 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 15 0.0 3 0 0 15 16 662 0.0 3 0 639 23 17 197702 0.0 3 193584 3834 128 156 18 367 0.0 3 23 331 11 2 19 1185 0.0 3 1077 20 75 13 20 111 0.0 3 0 3 0 108 21 1 0.0 3 0 0 1 22 2 0.0 3 2 23 1 0.0 3 1 25 1 0.0 3 1 26 2 0.0 3 2 28 1 0.0 3 0 1 29 1 0.0 3 0 1 31 1 0.0 3 1 33 1 0.0 3 1 34 1 0.0 3 1 35 1 0.0 3 1 37 139 0.0 3 0 0 0 139 38 15 0.0 3 0 0 1 14 39 2 0.0 3 0 0 0 2 41 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 56 7 0.0 3 0 0 0 7 64 4 0.0 3 0 0 0 4 65 5 0.0 3 0 0 0 5 68 3 0.0 3 0 0 0 3 77 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 1 85 2 0.0 3 0 0 0 2 86 919 0.0 3 0 0 2 917 87 136 0.0 3 0 0 0 136 88 105 0.0 3 0 0 0 105 89 124 0.0 3 0 0 6 118 90 20 0.0 3 0 0 1 19 91 5 0.0 3 0 0 0 5 92 12 0.0 3 0 0 0 12 93 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 95 4 0.0 3 0 0 0 4 96 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 6 0.0 3 0 0 0 6 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 9 0.0 3 0 0 0 9 104 4 0.0 3 0 0 0 4 105 2 0.0 3 0 0 1 1 106 11 0.0 3 1 0 0 10 107 16 0.0 3 0 0 0 16 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 10 0.0 3 0 0 0 10 112 29 0.0 3 0 0 0 29 113 7 0.0 3 0 0 0 7 118 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 131 16 0.0 3 0 0 0 16 134 2 0.0 3 0 0 0 2 137 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 1 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 3 0.0 3 0 0 0 3 158 39 0.0 3 0 0 1 38 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 166 6 0.0 3 0 0 0 6 167 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 182 3 0.0 3 0 0 0 3 183 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 11 0.0 3 0 0 0 11 189 13 0.0 3 0 0 0 13 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 197 3 0.0 3 0 0 0 3 198 273 0.0 3 0 0 0 273 199 20 0.0 3 0 0 1 19 200 24 0.0 3 0 0 2 22 201 51 0.0 3 0 0 0 51 202 41 0.0 3 0 0 4 37 203 24 0.0 3 0 0 0 24 204 5 0.0 3 0 0 0 5 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 207 4 0.0 3 0 0 1 3 208 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 213 10 0.0 3 0 0 0 10 214 16 0.0 3 0 0 0 16 215 2 0.0 3 0 0 0 2 216 21 0.0 3 0 0 1 20 217 1 0.0 3 0 0 0 1 221 2 0.0 3 0 1 0 1 222 1 0.0 3 0 1 226 4 0.0 3 0 0 0 4 227 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 5 0.0 3 0 0 0 5 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.21 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 201,713 Reads with adapters: 6,029 (3.0%) Reads written (passing filters): 201,713 (100.0%) Total basepairs processed: 55,213,485 bp Total written (filtered): 55,195,059 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6029 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.8% C: 7.8% G: 11.2% T: 1.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5721 3151.8 0 5721 4 288 787.9 0 288 5 13 197.0 0 13 6 6 49.2 0 6 10 1 0.2 1 0 1