This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.92 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 35,094 (1.3%) Reads that were too short: 8,896 (0.3%) Reads written (passing filters): 26,198 (1.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 6,030,384 bp (0.8%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 35094 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3525 642.2 0 3525 7 3627 642.2 0 3627 8 2648 642.2 0 2648 9 2173 642.2 0 2173 10 23 642.2 0 23 11 2 642.2 0 2 12 3 642.2 0 3 13 5 642.2 0 5 14 4 642.2 0 4 15 6 642.2 0 6 16 2 642.2 0 2 17 4 642.2 0 4 18 4 642.2 0 4 19 8 642.2 0 8 20 6 642.2 0 6 21 10 642.2 0 10 22 16 642.2 0 16 23 26 642.2 0 26 24 19 642.2 0 19 25 10 642.2 0 10 26 1 642.2 0 1 27 5 642.2 0 5 28 6 642.2 0 6 29 17 642.2 0 17 30 23 642.2 0 23 31 18 642.2 0 18 32 7 642.2 0 7 33 172 642.2 0 172 34 36 642.2 0 36 35 27 642.2 0 27 36 80 642.2 0 80 37 7 642.2 0 7 38 44 642.2 0 44 39 47 642.2 0 47 40 49 642.2 0 49 41 14 642.2 0 14 42 6 642.2 0 6 43 6 642.2 0 6 44 18 642.2 0 18 45 15 642.2 0 15 46 19 642.2 0 19 47 3 642.2 0 3 48 8 642.2 0 8 49 5 642.2 0 5 50 13 642.2 0 13 51 24 642.2 0 24 52 12 642.2 0 12 53 11 642.2 0 11 54 9 642.2 0 9 55 20 642.2 0 20 56 20 642.2 0 20 57 13 642.2 0 13 58 77 642.2 0 77 59 59 642.2 0 59 60 151 642.2 0 151 61 6 642.2 0 6 62 7 642.2 0 7 63 15 642.2 0 15 64 8 642.2 0 8 65 6 642.2 0 6 66 7 642.2 0 7 67 17 642.2 0 17 68 4 642.2 0 4 69 17 642.2 0 17 70 11 642.2 0 11 71 48 642.2 0 48 72 52 642.2 0 52 73 17 642.2 0 17 74 11 642.2 0 11 75 6 642.2 0 6 76 106 642.2 0 106 77 61 642.2 0 61 78 176 642.2 0 176 79 22 642.2 0 22 80 202 642.2 0 202 81 366 642.2 0 366 82 186 642.2 0 186 83 11 642.2 0 11 84 7 642.2 0 7 85 43 642.2 0 43 86 25 642.2 0 25 87 29 642.2 0 29 88 21 642.2 0 21 89 17 642.2 0 17 90 9 642.2 0 9 91 14 642.2 0 14 92 4 642.2 0 4 93 13 642.2 0 13 94 19 642.2 0 19 95 12 642.2 0 12 96 16 642.2 0 16 97 19 642.2 0 19 98 23 642.2 0 23 99 108 642.2 0 108 100 1265 642.2 0 1265 101 1484 642.2 0 1484 102 1281 642.2 0 1281 103 12 642.2 0 12 104 8 642.2 0 8 105 10 642.2 0 10 106 12 642.2 0 12 107 6 642.2 0 6 108 13 642.2 0 13 109 58 642.2 0 58 110 466 642.2 0 466 111 89 642.2 0 89 112 39 642.2 0 39 113 22 642.2 0 22 114 9 642.2 0 9 115 16 642.2 0 16 116 137 642.2 0 137 117 15 642.2 0 15 118 8 642.2 0 8 119 18 642.2 0 18 120 27 642.2 0 27 121 15 642.2 0 15 122 14 642.2 0 14 123 36 642.2 0 36 124 722 642.2 0 722 125 13 642.2 0 13 126 11 642.2 0 11 127 13 642.2 0 13 128 60 642.2 0 60 129 11 642.2 0 11 130 19 642.2 0 19 131 30 642.2 0 30 132 22 642.2 0 22 133 34 642.2 0 34 134 12 642.2 0 12 135 15 642.2 0 15 136 14 642.2 0 14 137 11 642.2 0 11 138 15 642.2 0 15 139 14 642.2 0 14 140 26 642.2 0 26 141 19 642.2 0 19 142 23 642.2 0 23 143 13 642.2 0 13 144 21 642.2 0 21 145 16 642.2 0 16 146 27 642.2 0 27 147 44 642.2 0 44 148 56 642.2 0 56 149 109 642.2 0 109 150 53 642.2 0 53 151 1097 642.2 0 1097 152 41 642.2 0 41 153 39 642.2 0 39 154 24 642.2 0 24 155 9 642.2 0 9 156 17 642.2 0 17 157 103 642.2 0 103 158 46 642.2 0 46 159 54 642.2 0 54 160 27 642.2 0 27 161 26 642.2 0 26 162 18 642.2 0 18 163 15 642.2 0 15 164 14 642.2 0 14 165 9 642.2 0 9 166 15 642.2 0 15 167 15 642.2 0 15 168 60 642.2 0 60 169 39 642.2 0 39 170 125 642.2 0 125 171 9 642.2 0 9 172 12 642.2 0 12 173 7 642.2 0 7 174 12 642.2 0 12 175 11 642.2 0 11 176 9 642.2 0 9 177 35 642.2 0 35 178 24 642.2 0 24 179 39 642.2 0 39 180 23 642.2 0 23 181 19 642.2 0 19 182 78 642.2 0 78 183 85 642.2 0 85 184 121 642.2 0 121 185 73 642.2 0 73 186 31 642.2 0 31 187 17 642.2 0 17 188 24 642.2 0 24 189 30 642.2 0 30 190 25 642.2 0 25 191 33 642.2 0 33 192 15 642.2 0 15 193 27 642.2 0 27 194 41 642.2 0 41 195 24 642.2 0 24 196 35 642.2 0 35 197 128 642.2 0 128 198 266 642.2 0 266 199 1317 642.2 0 1317 200 202 642.2 0 202 201 246 642.2 0 246 202 345 642.2 0 345 203 180 642.2 0 180 204 467 642.2 0 467 205 106 642.2 0 106 206 72 642.2 0 72 207 32 642.2 0 32 208 25 642.2 0 25 209 66 642.2 0 66 210 41 642.2 0 41 211 29 642.2 0 29 212 27 642.2 0 27 213 38 642.2 0 38 214 35 642.2 0 35 215 25 642.2 0 25 216 33 642.2 0 33 217 118 642.2 0 118 218 87 642.2 0 87 219 189 642.2 0 189 220 27 642.2 0 27 221 79 642.2 0 79 222 498 642.2 0 498 223 746 642.2 0 746 224 851 642.2 0 851 225 96 642.2 0 96 226 60 642.2 0 60 227 48 642.2 0 48 228 67 642.2 0 67 229 61 642.2 0 61 230 36 642.2 0 36 231 27 642.2 0 27 232 15 642.2 0 15 233 28 642.2 0 28 234 25 642.2 0 25 235 35 642.2 0 35 236 27 642.2 0 27 237 37 642.2 0 37 238 56 642.2 0 56 239 82 642.2 0 82 240 77 642.2 0 77 241 60 642.2 0 60 242 43 642.2 0 43 243 113 642.2 0 113 244 24 642.2 0 24 245 26 642.2 0 26 246 19 642.2 0 19 247 21 642.2 0 21 248 40 642.2 0 40 249 34 642.2 0 34 250 23 642.2 0 23 251 41 642.2 0 41 252 29 642.2 0 29 253 51 642.2 0 51 254 30 642.2 0 30 255 119 642.2 0 119 256 142 642.2 0 142 257 140 642.2 0 140 258 201 642.2 0 201 259 111 642.2 0 111 260 80 642.2 0 80 261 50 642.2 0 50 262 67 642.2 0 67 263 46 642.2 0 46 264 85 642.2 0 85 265 30 642.2 0 30 266 48 642.2 0 48 267 36 642.2 0 36 268 35 642.2 0 35 269 39 642.2 0 39 270 34 642.2 0 34 271 53 642.2 0 53 272 42 642.2 0 42 273 70 642.2 0 70 274 31 642.2 0 31 275 37 642.2 0 37 276 54 642.2 0 54 277 111 642.2 0 111 278 137 642.2 0 137 279 107 642.2 0 107 280 81 642.2 0 81 281 42 642.2 0 42 282 46 642.2 0 46 283 46 642.2 0 46 284 69 642.2 0 69 285 64 642.2 0 64 286 40 642.2 0 40 287 59 642.2 0 59 288 51 642.2 0 51 289 86 642.2 0 86 290 71 642.2 0 71 291 129 642.2 0 129 292 76 642.2 0 76 293 53 642.2 0 53 294 56 642.2 0 56 295 100 642.2 0 100 296 59 642.2 0 59 297 83 642.2 0 83 298 73 642.2 0 73 299 97 642.2 0 97 300 83 642.2 0 83 301 80 642.2 0 80 Finished in 1.27 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 26,198 Reads with adapters: 5,968 (22.8%) Reads that were too short: 16 (0.1%) Reads written (passing filters): 5,952 (22.7%) Total basepairs processed: 6,030,384 bp Total written (filtered): 1,616,892 bp (26.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5968 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 1 0.0 3 0 0 1 19 11 0.0 3 0 7 3 1 20 2252 0.0 4 2038 156 39 12 7 21 3581 0.0 4 3251 251 59 12 8 22 8 0.0 4 0 6 1 1 23 31 0.0 4 9 6 2 9 5 24 11 0.0 4 0 0 0 5 6 27 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 47 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 53 1 0.0 4 1 76 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 94 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 1 123 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 128 45 0.0 4 0 0 0 0 45 131 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 1 2 229 3 0.0 4 0 0 0 1 2 238 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 5,952 Reads with adapters: 1,342 (22.5%) Reads written (passing filters): 5,952 (100.0%) Total basepairs processed: 1,616,892 bp Total written (filtered): 1,602,689 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1342 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 9.2% G: 85.1% T: 3.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 93.0 0 13 4 1 23.2 0 1 7 1 0.4 0 1 9 71 0.0 0 10 61 10 478 0.0 1 34 444 11 767 0.0 1 32 735 12 7 0.0 1 0 7 13 2 0.0 1 0 2 19 1 0.0 1 1 168 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.