This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.18 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 22,835 (0.9%) Reads that were too short: 2,806 (0.1%) Reads written (passing filters): 20,029 (0.8%) Total basepairs processed: 791,719,999 bp Total written (filtered): 5,042,899 bp (0.6%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 22835 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3322 642.2 0 3322 7 4341 642.2 0 4341 8 2266 642.2 0 2266 9 2887 642.2 0 2887 10 12 642.2 0 12 11 1 642.2 0 1 13 3 642.2 0 3 14 2 642.2 0 2 15 3 642.2 0 3 16 2 642.2 0 2 17 1 642.2 0 1 18 4 642.2 0 4 19 1 642.2 0 1 20 3 642.2 0 3 21 3 642.2 0 3 22 2 642.2 0 2 23 5 642.2 0 5 25 3 642.2 0 3 26 3 642.2 0 3 27 4 642.2 0 4 28 1 642.2 0 1 29 1 642.2 0 1 30 13 642.2 0 13 31 4 642.2 0 4 32 4 642.2 0 4 33 1 642.2 0 1 34 6 642.2 0 6 35 15 642.2 0 15 36 78 642.2 0 78 37 3 642.2 0 3 38 23 642.2 0 23 39 27 642.2 0 27 40 11 642.2 0 11 41 9 642.2 0 9 42 2 642.2 0 2 43 5 642.2 0 5 44 27 642.2 0 27 45 6 642.2 0 6 46 22 642.2 0 22 47 6 642.2 0 6 48 2 642.2 0 2 49 4 642.2 0 4 50 3 642.2 0 3 51 11 642.2 0 11 52 9 642.2 0 9 53 11 642.2 0 11 54 5 642.2 0 5 55 7 642.2 0 7 56 6 642.2 0 6 57 9 642.2 0 9 58 19 642.2 0 19 59 8 642.2 0 8 60 39 642.2 0 39 61 4 642.2 0 4 62 5 642.2 0 5 63 10 642.2 0 10 64 7 642.2 0 7 65 2 642.2 0 2 66 7 642.2 0 7 67 8 642.2 0 8 68 4 642.2 0 4 69 7 642.2 0 7 70 4 642.2 0 4 71 45 642.2 0 45 72 56 642.2 0 56 73 8 642.2 0 8 74 3 642.2 0 3 75 5 642.2 0 5 76 79 642.2 0 79 77 66 642.2 0 66 78 156 642.2 0 156 79 12 642.2 0 12 80 61 642.2 0 61 81 213 642.2 0 213 82 43 642.2 0 43 83 6 642.2 0 6 84 3 642.2 0 3 85 9 642.2 0 9 86 10 642.2 0 10 87 4 642.2 0 4 88 5 642.2 0 5 89 15 642.2 0 15 90 5 642.2 0 5 91 7 642.2 0 7 92 2 642.2 0 2 93 13 642.2 0 13 94 3 642.2 0 3 95 15 642.2 0 15 96 3 642.2 0 3 97 2 642.2 0 2 98 14 642.2 0 14 99 24 642.2 0 24 100 529 642.2 0 529 101 530 642.2 0 530 102 242 642.2 0 242 103 8 642.2 0 8 104 6 642.2 0 6 105 8 642.2 0 8 106 5 642.2 0 5 107 8 642.2 0 8 108 9 642.2 0 9 109 35 642.2 0 35 110 564 642.2 0 564 111 22 642.2 0 22 112 12 642.2 0 12 113 29 642.2 0 29 114 10 642.2 0 10 115 12 642.2 0 12 116 135 642.2 0 135 117 8 642.2 0 8 118 6 642.2 0 6 119 11 642.2 0 11 120 7 642.2 0 7 121 8 642.2 0 8 122 4 642.2 0 4 123 22 642.2 0 22 124 702 642.2 0 702 125 7 642.2 0 7 126 5 642.2 0 5 127 8 642.2 0 8 128 55 642.2 0 55 129 2 642.2 0 2 130 2 642.2 0 2 131 11 642.2 0 11 132 7 642.2 0 7 133 8 642.2 0 8 134 7 642.2 0 7 135 6 642.2 0 6 136 3 642.2 0 3 137 6 642.2 0 6 138 4 642.2 0 4 139 6 642.2 0 6 140 19 642.2 0 19 141 7 642.2 0 7 142 10 642.2 0 10 143 11 642.2 0 11 144 17 642.2 0 17 145 8 642.2 0 8 146 18 642.2 0 18 147 23 642.2 0 23 148 23 642.2 0 23 149 54 642.2 0 54 150 39 642.2 0 39 151 1225 642.2 0 1225 152 42 642.2 0 42 153 32 642.2 0 32 154 11 642.2 0 11 155 2 642.2 0 2 156 8 642.2 0 8 157 22 642.2 0 22 158 23 642.2 0 23 159 16 642.2 0 16 160 4 642.2 0 4 161 8 642.2 0 8 162 9 642.2 0 9 163 6 642.2 0 6 164 5 642.2 0 5 165 6 642.2 0 6 166 6 642.2 0 6 167 4 642.2 0 4 168 26 642.2 0 26 169 16 642.2 0 16 170 121 642.2 0 121 171 8 642.2 0 8 172 6 642.2 0 6 173 8 642.2 0 8 174 6 642.2 0 6 175 6 642.2 0 6 176 8 642.2 0 8 177 9 642.2 0 9 178 9 642.2 0 9 179 6 642.2 0 6 180 3 642.2 0 3 181 11 642.2 0 11 182 25 642.2 0 25 183 54 642.2 0 54 184 17 642.2 0 17 185 13 642.2 0 13 186 4 642.2 0 4 187 5 642.2 0 5 188 11 642.2 0 11 189 11 642.2 0 11 190 19 642.2 0 19 191 65 642.2 0 65 192 7 642.2 0 7 193 9 642.2 0 9 194 31 642.2 0 31 195 9 642.2 0 9 196 13 642.2 0 13 197 40 642.2 0 40 198 73 642.2 0 73 199 322 642.2 0 322 200 86 642.2 0 86 201 35 642.2 0 35 202 37 642.2 0 37 203 28 642.2 0 28 204 80 642.2 0 80 205 21 642.2 0 21 206 19 642.2 0 19 207 8 642.2 0 8 208 12 642.2 0 12 209 20 642.2 0 20 210 14 642.2 0 14 211 14 642.2 0 14 212 13 642.2 0 13 213 7 642.2 0 7 214 17 642.2 0 17 215 22 642.2 0 22 216 7 642.2 0 7 217 28 642.2 0 28 218 28 642.2 0 28 219 51 642.2 0 51 220 13 642.2 0 13 221 28 642.2 0 28 222 80 642.2 0 80 223 219 642.2 0 219 224 346 642.2 0 346 225 25 642.2 0 25 226 42 642.2 0 42 227 9 642.2 0 9 228 57 642.2 0 57 229 17 642.2 0 17 230 16 642.2 0 16 231 14 642.2 0 14 232 4 642.2 0 4 233 9 642.2 0 9 234 11 642.2 0 11 235 11 642.2 0 11 236 8 642.2 0 8 237 13 642.2 0 13 238 12 642.2 0 12 239 34 642.2 0 34 240 18 642.2 0 18 241 27 642.2 0 27 242 19 642.2 0 19 243 31 642.2 0 31 244 16 642.2 0 16 245 13 642.2 0 13 246 11 642.2 0 11 247 13 642.2 0 13 248 11 642.2 0 11 249 10 642.2 0 10 250 14 642.2 0 14 251 18 642.2 0 18 252 8 642.2 0 8 253 15 642.2 0 15 254 10 642.2 0 10 255 21 642.2 0 21 256 27 642.2 0 27 257 33 642.2 0 33 258 53 642.2 0 53 259 71 642.2 0 71 260 27 642.2 0 27 261 13 642.2 0 13 262 19 642.2 0 19 263 14 642.2 0 14 264 36 642.2 0 36 265 9 642.2 0 9 266 10 642.2 0 10 267 11 642.2 0 11 268 14 642.2 0 14 269 21 642.2 0 21 270 13 642.2 0 13 271 14 642.2 0 14 272 14 642.2 0 14 273 23 642.2 0 23 274 11 642.2 0 11 275 8 642.2 0 8 276 19 642.2 0 19 277 12 642.2 0 12 278 27 642.2 0 27 279 21 642.2 0 21 280 24 642.2 0 24 281 7 642.2 0 7 282 18 642.2 0 18 283 15 642.2 0 15 284 25 642.2 0 25 285 16 642.2 0 16 286 12 642.2 0 12 287 19 642.2 0 19 288 28 642.2 0 28 289 36 642.2 0 36 290 23 642.2 0 23 291 55 642.2 0 55 292 37 642.2 0 37 293 23 642.2 0 23 294 22 642.2 0 22 295 84 642.2 0 84 296 34 642.2 0 34 297 28 642.2 0 28 298 27 642.2 0 27 299 48 642.2 0 48 300 39 642.2 0 39 301 17 642.2 0 17 Finished in 0.92 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 20,029 Reads with adapters: 5,457 (27.2%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 5,447 (27.2%) Total basepairs processed: 5,042,899 bp Total written (filtered): 1,506,005 bp (29.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5457 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 18 0.0 3 0 18 17 2213 0.0 3 2157 54 2 18 3194 0.0 3 3134 57 3 19 6 0.0 3 0 4 2 20 2 0.0 3 0 0 1 1 21 11 0.0 3 0 0 0 11 31 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 5,447 Reads with adapters: 5 (0.1%) Reads written (passing filters): 5,447 (100.0%) Total basepairs processed: 1,506,005 bp Total written (filtered): 1,505,987 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.0% C: 0.0% G: 80.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 85.1 0 3 4 1 21.3 0 1 5 1 5.3 0 1