This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.59 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 246,093 (9.4%) Reads that were too short: 5,534 (0.2%) Reads written (passing filters): 240,559 (9.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 43,056,828 bp (5.4%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 246093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 460 642.2 0 460 7 18824 642.2 0 18824 8 18431 642.2 0 18431 9 10 642.2 0 10 10 6 642.2 0 6 11 53 642.2 0 53 12 34 642.2 0 34 13 40 642.2 0 40 14 9 642.2 0 9 15 16 642.2 0 16 16 12 642.2 0 12 17 11 642.2 0 11 18 8 642.2 0 8 19 7 642.2 0 7 20 17 642.2 0 17 21 24 642.2 0 24 22 15 642.2 0 15 23 9 642.2 0 9 24 8 642.2 0 8 25 27 642.2 0 27 26 48 642.2 0 48 27 40 642.2 0 40 28 16 642.2 0 16 29 12 642.2 0 12 30 9 642.2 0 9 31 23 642.2 0 23 32 17 642.2 0 17 33 22 642.2 0 22 34 17 642.2 0 17 35 13 642.2 0 13 36 24 642.2 0 24 37 29 642.2 0 29 38 35 642.2 0 35 39 15 642.2 0 15 40 47 642.2 0 47 41 12 642.2 0 12 42 12 642.2 0 12 43 47 642.2 0 47 44 13 642.2 0 13 45 5 642.2 0 5 46 13 642.2 0 13 47 14 642.2 0 14 48 8 642.2 0 8 49 13 642.2 0 13 50 8 642.2 0 8 51 7 642.2 0 7 52 9 642.2 0 9 53 15 642.2 0 15 54 16 642.2 0 16 55 21 642.2 0 21 56 10 642.2 0 10 57 18 642.2 0 18 58 18 642.2 0 18 59 15 642.2 0 15 60 15 642.2 0 15 61 38 642.2 0 38 62 10 642.2 0 10 63 19 642.2 0 19 64 12 642.2 0 12 65 8 642.2 0 8 66 8 642.2 0 8 67 8 642.2 0 8 68 12 642.2 0 12 69 18 642.2 0 18 70 26 642.2 0 26 71 59 642.2 0 59 72 253 642.2 0 253 73 26 642.2 0 26 74 11 642.2 0 11 75 17 642.2 0 17 76 74 642.2 0 74 77 15 642.2 0 15 78 23 642.2 0 23 79 61 642.2 0 61 80 34 642.2 0 34 81 11 642.2 0 11 82 21 642.2 0 21 83 18 642.2 0 18 84 12 642.2 0 12 85 23 642.2 0 23 86 19 642.2 0 19 87 7 642.2 0 7 88 14 642.2 0 14 89 16 642.2 0 16 90 25 642.2 0 25 91 74 642.2 0 74 92 8 642.2 0 8 93 21 642.2 0 21 94 16 642.2 0 16 95 22 642.2 0 22 96 21 642.2 0 21 97 51 642.2 0 51 98 36 642.2 0 36 99 84 642.2 0 84 100 24 642.2 0 24 101 14 642.2 0 14 102 51 642.2 0 51 103 41 642.2 0 41 104 61 642.2 0 61 105 52 642.2 0 52 106 63 642.2 0 63 107 67 642.2 0 67 108 52 642.2 0 52 109 34 642.2 0 34 110 197 642.2 0 197 111 32 642.2 0 32 112 75 642.2 0 75 113 66 642.2 0 66 114 53 642.2 0 53 115 245 642.2 0 245 116 28 642.2 0 28 117 18 642.2 0 18 118 33 642.2 0 33 119 24 642.2 0 24 120 41 642.2 0 41 121 104 642.2 0 104 122 456 642.2 0 456 123 338 642.2 0 338 124 262 642.2 0 262 125 349 642.2 0 349 126 73 642.2 0 73 127 135 642.2 0 135 128 97 642.2 0 97 129 60 642.2 0 60 130 173 642.2 0 173 131 79 642.2 0 79 132 149 642.2 0 149 133 111 642.2 0 111 134 104 642.2 0 104 135 263 642.2 0 263 136 273 642.2 0 273 137 512 642.2 0 512 138 1111 642.2 0 1111 139 1177 642.2 0 1177 140 1654 642.2 0 1654 141 5192 642.2 0 5192 142 49997 642.2 0 49997 143 35022 642.2 0 35022 144 86314 642.2 0 86314 145 362 642.2 0 362 146 43 642.2 0 43 147 66 642.2 0 66 148 46 642.2 0 46 149 48 642.2 0 48 150 58 642.2 0 58 151 32 642.2 0 32 152 45 642.2 0 45 153 25 642.2 0 25 154 28 642.2 0 28 155 50 642.2 0 50 156 47 642.2 0 47 157 72 642.2 0 72 158 111 642.2 0 111 159 119 642.2 0 119 160 168 642.2 0 168 161 228 642.2 0 228 162 1984 642.2 0 1984 163 1995 642.2 0 1995 164 8540 642.2 0 8540 165 53 642.2 0 53 166 15 642.2 0 15 167 23 642.2 0 23 168 31 642.2 0 31 169 31 642.2 0 31 170 21 642.2 0 21 171 16 642.2 0 16 172 18 642.2 0 18 173 24 642.2 0 24 174 11 642.2 0 11 175 14 642.2 0 14 176 30 642.2 0 30 177 120 642.2 0 120 178 58 642.2 0 58 179 26 642.2 0 26 180 14 642.2 0 14 181 14 642.2 0 14 182 15 642.2 0 15 183 37 642.2 0 37 184 25 642.2 0 25 185 23 642.2 0 23 186 27 642.2 0 27 187 19 642.2 0 19 188 83 642.2 0 83 189 260 642.2 0 260 190 81 642.2 0 81 191 13 642.2 0 13 192 29 642.2 0 29 193 10 642.2 0 10 194 21 642.2 0 21 195 22 642.2 0 22 196 23 642.2 0 23 197 27 642.2 0 27 198 19 642.2 0 19 199 16 642.2 0 16 200 22 642.2 0 22 201 17 642.2 0 17 202 19 642.2 0 19 203 19 642.2 0 19 204 26 642.2 0 26 205 29 642.2 0 29 206 18 642.2 0 18 207 32 642.2 0 32 208 34 642.2 0 34 209 52 642.2 0 52 210 92 642.2 0 92 211 53 642.2 0 53 212 42 642.2 0 42 213 46 642.2 0 46 214 186 642.2 0 186 215 118 642.2 0 118 216 100 642.2 0 100 217 23 642.2 0 23 218 25 642.2 0 25 219 13 642.2 0 13 220 25 642.2 0 25 221 27 642.2 0 27 222 40 642.2 0 40 223 65 642.2 0 65 224 64 642.2 0 64 225 146 642.2 0 146 226 83 642.2 0 83 227 49 642.2 0 49 228 22 642.2 0 22 229 21 642.2 0 21 230 28 642.2 0 28 231 15 642.2 0 15 232 55 642.2 0 55 233 70 642.2 0 70 234 82 642.2 0 82 235 21 642.2 0 21 236 48 642.2 0 48 237 24 642.2 0 24 238 40 642.2 0 40 239 100 642.2 0 100 240 38 642.2 0 38 241 34 642.2 0 34 242 22 642.2 0 22 243 25 642.2 0 25 244 35 642.2 0 35 245 37 642.2 0 37 246 32 642.2 0 32 247 19 642.2 0 19 248 25 642.2 0 25 249 20 642.2 0 20 250 13 642.2 0 13 251 37 642.2 0 37 252 26 642.2 0 26 253 21 642.2 0 21 254 21 642.2 0 21 255 39 642.2 0 39 256 41 642.2 0 41 257 56 642.2 0 56 258 53 642.2 0 53 259 43 642.2 0 43 260 67 642.2 0 67 261 197 642.2 0 197 262 89 642.2 0 89 263 392 642.2 0 392 264 94 642.2 0 94 265 49 642.2 0 49 266 27 642.2 0 27 267 35 642.2 0 35 268 36 642.2 0 36 269 56 642.2 0 56 270 59 642.2 0 59 271 49 642.2 0 49 272 24 642.2 0 24 273 41 642.2 0 41 274 44 642.2 0 44 275 52 642.2 0 52 276 48 642.2 0 48 277 54 642.2 0 54 278 49 642.2 0 49 279 163 642.2 0 163 280 144 642.2 0 144 281 280 642.2 0 280 282 76 642.2 0 76 283 63 642.2 0 63 284 61 642.2 0 61 285 77 642.2 0 77 286 36 642.2 0 36 287 53 642.2 0 53 288 23 642.2 0 23 289 33 642.2 0 33 290 33 642.2 0 33 291 29 642.2 0 29 292 45 642.2 0 45 293 34 642.2 0 34 294 45 642.2 0 45 295 82 642.2 0 82 296 48 642.2 0 48 297 57 642.2 0 57 298 38 642.2 0 38 299 21 642.2 0 21 300 34 642.2 0 34 301 8 642.2 0 8 Finished in 8.89 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 240,559 Reads with adapters: 19,167 (8.0%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 19,134 (8.0%) Total basepairs processed: 43,056,828 bp Total written (filtered): 5,196,308 bp (12.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19167 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 12 0.0 3 0 0 7 2 3 19 130 0.0 3 0 84 21 16 9 20 18613 0.0 4 17158 1131 233 69 22 21 54 0.0 4 5 40 4 2 3 22 5 0.0 4 1 0 2 2 23 65 0.0 4 0 0 0 45 20 24 2 0.0 4 1 0 0 0 1 36 2 0.0 4 0 0 0 0 2 37 12 0.0 4 0 0 0 0 12 38 5 0.0 4 0 0 0 0 5 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 47 1 0.0 4 1 57 1 0.0 4 0 0 0 1 61 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 81 3 0.0 4 0 0 0 0 3 92 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 0 5 127 220 0.0 4 0 0 0 0 220 132 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 3 0.0 4 0 0 0 0 3 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.70 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 19,134 Reads with adapters: 4,542 (23.7%) Reads written (passing filters): 19,134 (100.0%) Total basepairs processed: 5,196,308 bp Total written (filtered): 5,140,822 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4542 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 10.0% G: 84.6% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 24 299.0 0 24 4 6 74.7 0 6 9 141 0.1 0 8 133 10 916 0.0 1 89 827 11 3385 0.0 1 101 3284 12 27 0.0 1 1 26 13 3 0.0 1 0 3 14 6 0.0 1 0 6 15 1 0.0 1 0 1 170 1 0.0 1 1 209 5 0.0 1 4 1 224 26 0.0 1 20 6 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.