This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.36 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 280,792 (10.7%) Reads that were too short: 3,717 (0.1%) Reads written (passing filters): 277,075 (10.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 49,169,922 bp (6.2%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 280792 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 440 642.2 0 440 7 18404 642.2 0 18404 8 21260 642.2 0 21260 9 17 642.2 0 17 10 12 642.2 0 12 11 18 642.2 0 18 12 16 642.2 0 16 13 14 642.2 0 14 14 9 642.2 0 9 15 13 642.2 0 13 16 5 642.2 0 5 17 5 642.2 0 5 18 12 642.2 0 12 19 8 642.2 0 8 20 13 642.2 0 13 21 20 642.2 0 20 22 9 642.2 0 9 23 5 642.2 0 5 24 11 642.2 0 11 25 7 642.2 0 7 26 10 642.2 0 10 27 24 642.2 0 24 28 13 642.2 0 13 29 10 642.2 0 10 30 10 642.2 0 10 31 13 642.2 0 13 32 7 642.2 0 7 33 11 642.2 0 11 34 9 642.2 0 9 35 9 642.2 0 9 36 7 642.2 0 7 37 8 642.2 0 8 38 3 642.2 0 3 39 6 642.2 0 6 40 9 642.2 0 9 41 9 642.2 0 9 42 10 642.2 0 10 43 47 642.2 0 47 44 11 642.2 0 11 45 5 642.2 0 5 46 16 642.2 0 16 47 6 642.2 0 6 48 6 642.2 0 6 49 6 642.2 0 6 50 1 642.2 0 1 51 4 642.2 0 4 52 5 642.2 0 5 53 8 642.2 0 8 54 12 642.2 0 12 55 13 642.2 0 13 56 5 642.2 0 5 57 22 642.2 0 22 58 13 642.2 0 13 59 14 642.2 0 14 60 11 642.2 0 11 61 42 642.2 0 42 62 1 642.2 0 1 63 19 642.2 0 19 64 15 642.2 0 15 65 7 642.2 0 7 66 9 642.2 0 9 67 11 642.2 0 11 68 13 642.2 0 13 69 18 642.2 0 18 70 14 642.2 0 14 71 29 642.2 0 29 72 296 642.2 0 296 73 20 642.2 0 20 74 11 642.2 0 11 75 19 642.2 0 19 76 47 642.2 0 47 77 8 642.2 0 8 78 9 642.2 0 9 79 73 642.2 0 73 80 22 642.2 0 22 81 10 642.2 0 10 82 26 642.2 0 26 83 14 642.2 0 14 84 14 642.2 0 14 85 11 642.2 0 11 86 20 642.2 0 20 87 17 642.2 0 17 88 15 642.2 0 15 89 33 642.2 0 33 90 23 642.2 0 23 91 102 642.2 0 102 92 23 642.2 0 23 93 22 642.2 0 22 94 16 642.2 0 16 95 36 642.2 0 36 96 18 642.2 0 18 97 50 642.2 0 50 98 36 642.2 0 36 99 61 642.2 0 61 100 25 642.2 0 25 101 18 642.2 0 18 102 37 642.2 0 37 103 35 642.2 0 35 104 41 642.2 0 41 105 70 642.2 0 70 106 62 642.2 0 62 107 60 642.2 0 60 108 46 642.2 0 46 109 31 642.2 0 31 110 175 642.2 0 175 111 30 642.2 0 30 112 57 642.2 0 57 113 28 642.2 0 28 114 43 642.2 0 43 115 272 642.2 0 272 116 15 642.2 0 15 117 14 642.2 0 14 118 33 642.2 0 33 119 15 642.2 0 15 120 34 642.2 0 34 121 53 642.2 0 53 122 382 642.2 0 382 123 221 642.2 0 221 124 202 642.2 0 202 125 398 642.2 0 398 126 62 642.2 0 62 127 129 642.2 0 129 128 96 642.2 0 96 129 60 642.2 0 60 130 176 642.2 0 176 131 102 642.2 0 102 132 148 642.2 0 148 133 135 642.2 0 135 134 114 642.2 0 114 135 222 642.2 0 222 136 280 642.2 0 280 137 555 642.2 0 555 138 1300 642.2 0 1300 139 1352 642.2 0 1352 140 1696 642.2 0 1696 141 4748 642.2 0 4748 142 60262 642.2 0 60262 143 43426 642.2 0 43426 144 106495 642.2 0 106495 145 414 642.2 0 414 146 40 642.2 0 40 147 47 642.2 0 47 148 28 642.2 0 28 149 33 642.2 0 33 150 44 642.2 0 44 151 25 642.2 0 25 152 25 642.2 0 25 153 21 642.2 0 21 154 14 642.2 0 14 155 24 642.2 0 24 156 23 642.2 0 23 157 47 642.2 0 47 158 82 642.2 0 82 159 67 642.2 0 67 160 107 642.2 0 107 161 170 642.2 0 170 162 1824 642.2 0 1824 163 1742 642.2 0 1742 164 5580 642.2 0 5580 165 21 642.2 0 21 166 9 642.2 0 9 167 15 642.2 0 15 168 17 642.2 0 17 169 30 642.2 0 30 170 18 642.2 0 18 171 12 642.2 0 12 172 16 642.2 0 16 173 8 642.2 0 8 174 11 642.2 0 11 175 19 642.2 0 19 176 27 642.2 0 27 177 83 642.2 0 83 178 30 642.2 0 30 179 16 642.2 0 16 180 12 642.2 0 12 181 7 642.2 0 7 182 18 642.2 0 18 183 17 642.2 0 17 184 19 642.2 0 19 185 24 642.2 0 24 186 15 642.2 0 15 187 18 642.2 0 18 188 77 642.2 0 77 189 305 642.2 0 305 190 65 642.2 0 65 191 11 642.2 0 11 192 21 642.2 0 21 193 12 642.2 0 12 194 11 642.2 0 11 195 18 642.2 0 18 196 17 642.2 0 17 197 12 642.2 0 12 198 14 642.2 0 14 199 13 642.2 0 13 200 10 642.2 0 10 201 14 642.2 0 14 202 19 642.2 0 19 203 12 642.2 0 12 204 16 642.2 0 16 205 31 642.2 0 31 206 21 642.2 0 21 207 18 642.2 0 18 208 27 642.2 0 27 209 52 642.2 0 52 210 69 642.2 0 69 211 26 642.2 0 26 212 31 642.2 0 31 213 30 642.2 0 30 214 80 642.2 0 80 215 63 642.2 0 63 216 69 642.2 0 69 217 21 642.2 0 21 218 15 642.2 0 15 219 8 642.2 0 8 220 16 642.2 0 16 221 21 642.2 0 21 222 25 642.2 0 25 223 25 642.2 0 25 224 29 642.2 0 29 225 121 642.2 0 121 226 36 642.2 0 36 227 43 642.2 0 43 228 20 642.2 0 20 229 11 642.2 0 11 230 14 642.2 0 14 231 22 642.2 0 22 232 24 642.2 0 24 233 34 642.2 0 34 234 45 642.2 0 45 235 24 642.2 0 24 236 31 642.2 0 31 237 28 642.2 0 28 238 35 642.2 0 35 239 94 642.2 0 94 240 29 642.2 0 29 241 20 642.2 0 20 242 19 642.2 0 19 243 21 642.2 0 21 244 29 642.2 0 29 245 32 642.2 0 32 246 32 642.2 0 32 247 22 642.2 0 22 248 14 642.2 0 14 249 22 642.2 0 22 250 14 642.2 0 14 251 11 642.2 0 11 252 18 642.2 0 18 253 20 642.2 0 20 254 17 642.2 0 17 255 32 642.2 0 32 256 44 642.2 0 44 257 33 642.2 0 33 258 32 642.2 0 32 259 37 642.2 0 37 260 38 642.2 0 38 261 100 642.2 0 100 262 61 642.2 0 61 263 265 642.2 0 265 264 57 642.2 0 57 265 22 642.2 0 22 266 20 642.2 0 20 267 18 642.2 0 18 268 23 642.2 0 23 269 40 642.2 0 40 270 33 642.2 0 33 271 25 642.2 0 25 272 24 642.2 0 24 273 22 642.2 0 22 274 24 642.2 0 24 275 34 642.2 0 34 276 44 642.2 0 44 277 27 642.2 0 27 278 30 642.2 0 30 279 107 642.2 0 107 280 89 642.2 0 89 281 195 642.2 0 195 282 44 642.2 0 44 283 44 642.2 0 44 284 37 642.2 0 37 285 34 642.2 0 34 286 32 642.2 0 32 287 26 642.2 0 26 288 27 642.2 0 27 289 21 642.2 0 21 290 23 642.2 0 23 291 15 642.2 0 15 292 24 642.2 0 24 293 23 642.2 0 23 294 31 642.2 0 31 295 55 642.2 0 55 296 40 642.2 0 40 297 57 642.2 0 57 298 26 642.2 0 26 299 25 642.2 0 25 300 16 642.2 0 16 301 15 642.2 0 15 Finished in 9.04 s (33 us/read; 1.84 M reads/minute). === Summary === Total reads processed: 277,075 Reads with adapters: 18,265 (6.6%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 18,248 (6.6%) Total basepairs processed: 49,169,922 bp Total written (filtered): 5,053,917 bp (10.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18265 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 77 0.0 3 0 76 1 17 18102 0.0 3 17758 335 7 2 18 50 0.0 3 1 48 1 19 7 0.0 3 0 1 6 20 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 107 1 0.0 3 0 1 108 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 152 1 0.0 3 1 164 2 0.0 3 0 0 0 2 172 1 0.0 3 1 206 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.64 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 18,248 Reads with adapters: 17 (0.1%) Reads written (passing filters): 18,248 (100.0%) Total basepairs processed: 5,053,917 bp Total written (filtered): 5,053,858 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 17 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.3% C: 11.8% G: 41.2% T: 11.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 12 285.1 0 12 4 3 71.3 0 3 5 1 17.8 0 1 6 1 4.5 0 1