This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.63 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 103,314 (3.9%) Reads that were too short: 33,038 (1.3%) Reads written (passing filters): 70,276 (2.7%) Total basepairs processed: 791,719,999 bp Total written (filtered): 13,540,669 bp (1.7%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 103314 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 239 642.2 0 239 7 7944 642.2 0 7944 8 267 642.2 0 267 9 7580 642.2 0 7580 10 126 642.2 0 126 11 119 642.2 0 119 12 188 642.2 0 188 13 113 642.2 0 113 14 96 642.2 0 96 15 98 642.2 0 98 16 93 642.2 0 93 17 95 642.2 0 95 18 88 642.2 0 88 19 87 642.2 0 87 20 122 642.2 0 122 21 123 642.2 0 123 22 103 642.2 0 103 23 103 642.2 0 103 24 106 642.2 0 106 25 117 642.2 0 117 26 93 642.2 0 93 27 127 642.2 0 127 28 95 642.2 0 95 29 170 642.2 0 170 30 152 642.2 0 152 31 305 642.2 0 305 32 478 642.2 0 478 33 440 642.2 0 440 34 106 642.2 0 106 35 280 642.2 0 280 36 109 642.2 0 109 37 193 642.2 0 193 38 116 642.2 0 116 39 112 642.2 0 112 40 114 642.2 0 114 41 109 642.2 0 109 42 82 642.2 0 82 43 85 642.2 0 85 44 86 642.2 0 86 45 101 642.2 0 101 46 116 642.2 0 116 47 103 642.2 0 103 48 106 642.2 0 106 49 92 642.2 0 92 50 115 642.2 0 115 51 177 642.2 0 177 52 93 642.2 0 93 53 105 642.2 0 105 54 94 642.2 0 94 55 90 642.2 0 90 56 102 642.2 0 102 57 76 642.2 0 76 58 106 642.2 0 106 59 105 642.2 0 105 60 104 642.2 0 104 61 93 642.2 0 93 62 84 642.2 0 84 63 80 642.2 0 80 64 112 642.2 0 112 65 128 642.2 0 128 66 102 642.2 0 102 67 153 642.2 0 153 68 280 642.2 0 280 69 426 642.2 0 426 70 426 642.2 0 426 71 103 642.2 0 103 72 113 642.2 0 113 73 158 642.2 0 158 74 120 642.2 0 120 75 135 642.2 0 135 76 127 642.2 0 127 77 114 642.2 0 114 78 119 642.2 0 119 79 136 642.2 0 136 80 178 642.2 0 178 81 140 642.2 0 140 82 150 642.2 0 150 83 119 642.2 0 119 84 132 642.2 0 132 85 132 642.2 0 132 86 134 642.2 0 134 87 113 642.2 0 113 88 144 642.2 0 144 89 118 642.2 0 118 90 126 642.2 0 126 91 116 642.2 0 116 92 130 642.2 0 130 93 113 642.2 0 113 94 109 642.2 0 109 95 185 642.2 0 185 96 94 642.2 0 94 97 113 642.2 0 113 98 229 642.2 0 229 99 244 642.2 0 244 100 194 642.2 0 194 101 115 642.2 0 115 102 167 642.2 0 167 103 130 642.2 0 130 104 116 642.2 0 116 105 133 642.2 0 133 106 105 642.2 0 105 107 115 642.2 0 115 108 125 642.2 0 125 109 133 642.2 0 133 110 123 642.2 0 123 111 396 642.2 0 396 112 125 642.2 0 125 113 252 642.2 0 252 114 200 642.2 0 200 115 138 642.2 0 138 116 318 642.2 0 318 117 640 642.2 0 640 118 352 642.2 0 352 119 307 642.2 0 307 120 172 642.2 0 172 121 217 642.2 0 217 122 181 642.2 0 181 123 346 642.2 0 346 124 244 642.2 0 244 125 136 642.2 0 136 126 138 642.2 0 138 127 154 642.2 0 154 128 192 642.2 0 192 129 379 642.2 0 379 130 216 642.2 0 216 131 331 642.2 0 331 132 281 642.2 0 281 133 1873 642.2 0 1873 134 821 642.2 0 821 135 1258 642.2 0 1258 136 510 642.2 0 510 137 276 642.2 0 276 138 157 642.2 0 157 139 252 642.2 0 252 140 112 642.2 0 112 141 266 642.2 0 266 142 139 642.2 0 139 143 139 642.2 0 139 144 250 642.2 0 250 145 247 642.2 0 247 146 238 642.2 0 238 147 169 642.2 0 169 148 323 642.2 0 323 149 123 642.2 0 123 150 154 642.2 0 154 151 233 642.2 0 233 152 1058 642.2 0 1058 153 346 642.2 0 346 154 353 642.2 0 353 155 409 642.2 0 409 156 334 642.2 0 334 157 588 642.2 0 588 158 193 642.2 0 193 159 280 642.2 0 280 160 606 642.2 0 606 161 130 642.2 0 130 162 201 642.2 0 201 163 183 642.2 0 183 164 370 642.2 0 370 165 1209 642.2 0 1209 166 374 642.2 0 374 167 319 642.2 0 319 168 272 642.2 0 272 169 324 642.2 0 324 170 186 642.2 0 186 171 139 642.2 0 139 172 167 642.2 0 167 173 120 642.2 0 120 174 166 642.2 0 166 175 439 642.2 0 439 176 343 642.2 0 343 177 932 642.2 0 932 178 545 642.2 0 545 179 941 642.2 0 941 180 817 642.2 0 817 181 577 642.2 0 577 182 1605 642.2 0 1605 183 509 642.2 0 509 184 302 642.2 0 302 185 172 642.2 0 172 186 208 642.2 0 208 187 211 642.2 0 211 188 171 642.2 0 171 189 117 642.2 0 117 190 135 642.2 0 135 191 143 642.2 0 143 192 270 642.2 0 270 193 181 642.2 0 181 194 548 642.2 0 548 195 704 642.2 0 704 196 861 642.2 0 861 197 943 642.2 0 943 198 373 642.2 0 373 199 365 642.2 0 365 200 1650 642.2 0 1650 201 4604 642.2 0 4604 202 2863 642.2 0 2863 203 777 642.2 0 777 204 97 642.2 0 97 205 177 642.2 0 177 206 167 642.2 0 167 207 343 642.2 0 343 208 119 642.2 0 119 209 138 642.2 0 138 210 113 642.2 0 113 211 113 642.2 0 113 212 116 642.2 0 116 213 211 642.2 0 211 214 602 642.2 0 602 215 137 642.2 0 137 216 112 642.2 0 112 217 130 642.2 0 130 218 113 642.2 0 113 219 164 642.2 0 164 220 152 642.2 0 152 221 313 642.2 0 313 222 532 642.2 0 532 223 260 642.2 0 260 224 203 642.2 0 203 225 123 642.2 0 123 226 315 642.2 0 315 227 304 642.2 0 304 228 132 642.2 0 132 229 106 642.2 0 106 230 111 642.2 0 111 231 155 642.2 0 155 232 161 642.2 0 161 233 158 642.2 0 158 234 169 642.2 0 169 235 133 642.2 0 133 236 147 642.2 0 147 237 128 642.2 0 128 238 134 642.2 0 134 239 158 642.2 0 158 240 496 642.2 0 496 241 280 642.2 0 280 242 262 642.2 0 262 243 180 642.2 0 180 244 405 642.2 0 405 245 143 642.2 0 143 246 187 642.2 0 187 247 129 642.2 0 129 248 141 642.2 0 141 249 166 642.2 0 166 250 242 642.2 0 242 251 432 642.2 0 432 252 265 642.2 0 265 253 358 642.2 0 358 254 352 642.2 0 352 255 291 642.2 0 291 256 305 642.2 0 305 257 361 642.2 0 361 258 655 642.2 0 655 259 6528 642.2 0 6528 260 410 642.2 0 410 261 186 642.2 0 186 262 255 642.2 0 255 263 559 642.2 0 559 264 641 642.2 0 641 265 537 642.2 0 537 266 621 642.2 0 621 267 129 642.2 0 129 268 303 642.2 0 303 269 222 642.2 0 222 270 227 642.2 0 227 271 162 642.2 0 162 272 158 642.2 0 158 273 120 642.2 0 120 274 396 642.2 0 396 275 342 642.2 0 342 276 511 642.2 0 511 277 566 642.2 0 566 278 653 642.2 0 653 279 123 642.2 0 123 280 95 642.2 0 95 281 237 642.2 0 237 282 110 642.2 0 110 283 112 642.2 0 112 284 125 642.2 0 125 285 139 642.2 0 139 286 113 642.2 0 113 287 111 642.2 0 111 288 113 642.2 0 113 289 194 642.2 0 194 290 218 642.2 0 218 291 117 642.2 0 117 292 153 642.2 0 153 293 134 642.2 0 134 294 262 642.2 0 262 295 115 642.2 0 115 296 118 642.2 0 118 297 137 642.2 0 137 298 183 642.2 0 183 299 181 642.2 0 181 300 153 642.2 0 153 301 233 642.2 0 233 Finished in 2.91 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 70,276 Reads with adapters: 7,916 (11.3%) Reads that were too short: 61 (0.1%) Reads written (passing filters): 7,855 (11.2%) Total basepairs processed: 13,540,669 bp Total written (filtered): 2,147,813 bp (15.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7916 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 19 67 0.0 3 0 47 8 7 5 20 7692 0.0 4 7187 412 66 19 8 21 23 0.0 4 3 18 0 0 2 22 9 0.0 4 2 2 5 23 25 0.0 4 3 3 0 14 5 26 3 0.0 4 0 0 0 0 3 28 1 0.0 4 0 1 30 2 0.0 4 1 0 0 0 1 32 7 0.0 4 2 2 1 2 33 3 0.0 4 1 1 1 34 4 0.0 4 2 2 35 1 0.0 4 1 36 2 0.0 4 0 0 0 0 2 40 8 0.0 4 0 0 0 0 8 41 6 0.0 4 0 0 0 0 6 42 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 1 65 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 1 77 2 0.0 4 0 0 0 0 2 85 2 0.0 4 0 0 0 1 1 86 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 89 3 0.0 4 0 0 0 0 3 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 99 2 0.0 4 0 0 0 0 2 103 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 106 2 0.0 4 0 0 0 0 2 114 2 0.0 4 0 0 0 0 2 116 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 1 126 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 1 1 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 282 3 0.0 4 0 0 0 0 3 285 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 7,855 Reads with adapters: 593 (7.5%) Reads written (passing filters): 7,855 (100.0%) Total basepairs processed: 2,147,813 bp Total written (filtered): 2,119,080 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 593 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.3% C: 36.4% G: 53.3% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 47 122.7 0 47 4 11 30.7 0 11 9 4 0.0 0 0 4 10 4 0.0 1 0 4 11 259 0.0 1 7 252 12 89 0.0 1 6 83 13 3 0.0 1 0 3 124 145 0.0 1 114 31 125 2 0.0 1 2 152 2 0.0 1 2 153 1 0.0 1 1 210 1 0.0 1 1 222 2 0.0 1 1 1 225 23 0.0 1 20 3