This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.23 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 89,905 (3.4%) Reads that were too short: 31,871 (1.2%) Reads written (passing filters): 58,034 (2.2%) Total basepairs processed: 791,719,999 bp Total written (filtered): 11,692,031 bp (1.5%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 89905 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 172 642.2 0 172 7 9013 642.2 0 9013 8 210 642.2 0 210 9 7426 642.2 0 7426 10 80 642.2 0 80 11 75 642.2 0 75 12 63 642.2 0 63 13 64 642.2 0 64 14 80 642.2 0 80 15 82 642.2 0 82 16 72 642.2 0 72 17 82 642.2 0 82 18 64 642.2 0 64 19 63 642.2 0 63 20 81 642.2 0 81 21 66 642.2 0 66 22 70 642.2 0 70 23 72 642.2 0 72 24 75 642.2 0 75 25 43 642.2 0 43 26 83 642.2 0 83 27 77 642.2 0 77 28 63 642.2 0 63 29 151 642.2 0 151 30 140 642.2 0 140 31 98 642.2 0 98 32 134 642.2 0 134 33 153 642.2 0 153 34 100 642.2 0 100 35 255 642.2 0 255 36 82 642.2 0 82 37 201 642.2 0 201 38 98 642.2 0 98 39 85 642.2 0 85 40 72 642.2 0 72 41 83 642.2 0 83 42 75 642.2 0 75 43 73 642.2 0 73 44 79 642.2 0 79 45 78 642.2 0 78 46 82 642.2 0 82 47 77 642.2 0 77 48 90 642.2 0 90 49 74 642.2 0 74 50 74 642.2 0 74 51 161 642.2 0 161 52 104 642.2 0 104 53 75 642.2 0 75 54 75 642.2 0 75 55 87 642.2 0 87 56 90 642.2 0 90 57 80 642.2 0 80 58 90 642.2 0 90 59 101 642.2 0 101 60 82 642.2 0 82 61 90 642.2 0 90 62 65 642.2 0 65 63 85 642.2 0 85 64 90 642.2 0 90 65 103 642.2 0 103 66 86 642.2 0 86 67 140 642.2 0 140 68 318 642.2 0 318 69 417 642.2 0 417 70 452 642.2 0 452 71 85 642.2 0 85 72 82 642.2 0 82 73 103 642.2 0 103 74 77 642.2 0 77 75 82 642.2 0 82 76 107 642.2 0 107 77 87 642.2 0 87 78 76 642.2 0 76 79 107 642.2 0 107 80 133 642.2 0 133 81 104 642.2 0 104 82 98 642.2 0 98 83 123 642.2 0 123 84 108 642.2 0 108 85 100 642.2 0 100 86 86 642.2 0 86 87 95 642.2 0 95 88 116 642.2 0 116 89 105 642.2 0 105 90 97 642.2 0 97 91 101 642.2 0 101 92 112 642.2 0 112 93 102 642.2 0 102 94 107 642.2 0 107 95 171 642.2 0 171 96 83 642.2 0 83 97 89 642.2 0 89 98 152 642.2 0 152 99 159 642.2 0 159 100 123 642.2 0 123 101 106 642.2 0 106 102 175 642.2 0 175 103 111 642.2 0 111 104 88 642.2 0 88 105 109 642.2 0 109 106 86 642.2 0 86 107 102 642.2 0 102 108 101 642.2 0 101 109 100 642.2 0 100 110 109 642.2 0 109 111 376 642.2 0 376 112 89 642.2 0 89 113 151 642.2 0 151 114 260 642.2 0 260 115 138 642.2 0 138 116 197 642.2 0 197 117 398 642.2 0 398 118 169 642.2 0 169 119 284 642.2 0 284 120 133 642.2 0 133 121 178 642.2 0 178 122 148 642.2 0 148 123 314 642.2 0 314 124 204 642.2 0 204 125 125 642.2 0 125 126 126 642.2 0 126 127 106 642.2 0 106 128 171 642.2 0 171 129 362 642.2 0 362 130 116 642.2 0 116 131 248 642.2 0 248 132 154 642.2 0 154 133 1737 642.2 0 1737 134 608 642.2 0 608 135 745 642.2 0 745 136 233 642.2 0 233 137 206 642.2 0 206 138 138 642.2 0 138 139 196 642.2 0 196 140 94 642.2 0 94 141 271 642.2 0 271 142 111 642.2 0 111 143 96 642.2 0 96 144 193 642.2 0 193 145 245 642.2 0 245 146 164 642.2 0 164 147 139 642.2 0 139 148 289 642.2 0 289 149 98 642.2 0 98 150 146 642.2 0 146 151 187 642.2 0 187 152 1104 642.2 0 1104 153 343 642.2 0 343 154 354 642.2 0 354 155 218 642.2 0 218 156 219 642.2 0 219 157 202 642.2 0 202 158 146 642.2 0 146 159 273 642.2 0 273 160 689 642.2 0 689 161 115 642.2 0 115 162 208 642.2 0 208 163 170 642.2 0 170 164 379 642.2 0 379 165 1247 642.2 0 1247 166 395 642.2 0 395 167 252 642.2 0 252 168 219 642.2 0 219 169 285 642.2 0 285 170 147 642.2 0 147 171 117 642.2 0 117 172 178 642.2 0 178 173 106 642.2 0 106 174 122 642.2 0 122 175 323 642.2 0 323 176 267 642.2 0 267 177 481 642.2 0 481 178 457 642.2 0 457 179 675 642.2 0 675 180 574 642.2 0 574 181 381 642.2 0 381 182 1161 642.2 0 1161 183 346 642.2 0 346 184 284 642.2 0 284 185 180 642.2 0 180 186 227 642.2 0 227 187 141 642.2 0 141 188 126 642.2 0 126 189 118 642.2 0 118 190 154 642.2 0 154 191 137 642.2 0 137 192 320 642.2 0 320 193 120 642.2 0 120 194 538 642.2 0 538 195 321 642.2 0 321 196 355 642.2 0 355 197 435 642.2 0 435 198 280 642.2 0 280 199 327 642.2 0 327 200 832 642.2 0 832 201 3065 642.2 0 3065 202 1748 642.2 0 1748 203 439 642.2 0 439 204 80 642.2 0 80 205 166 642.2 0 166 206 187 642.2 0 187 207 352 642.2 0 352 208 103 642.2 0 103 209 127 642.2 0 127 210 110 642.2 0 110 211 83 642.2 0 83 212 117 642.2 0 117 213 232 642.2 0 232 214 662 642.2 0 662 215 146 642.2 0 146 216 112 642.2 0 112 217 123 642.2 0 123 218 78 642.2 0 78 219 115 642.2 0 115 220 89 642.2 0 89 221 193 642.2 0 193 222 276 642.2 0 276 223 128 642.2 0 128 224 145 642.2 0 145 225 120 642.2 0 120 226 342 642.2 0 342 227 373 642.2 0 373 228 112 642.2 0 112 229 132 642.2 0 132 230 120 642.2 0 120 231 138 642.2 0 138 232 137 642.2 0 137 233 153 642.2 0 153 234 147 642.2 0 147 235 123 642.2 0 123 236 144 642.2 0 144 237 128 642.2 0 128 238 129 642.2 0 129 239 143 642.2 0 143 240 595 642.2 0 595 241 268 642.2 0 268 242 143 642.2 0 143 243 154 642.2 0 154 244 457 642.2 0 457 245 165 642.2 0 165 246 248 642.2 0 248 247 139 642.2 0 139 248 153 642.2 0 153 249 147 642.2 0 147 250 284 642.2 0 284 251 523 642.2 0 523 252 269 642.2 0 269 253 333 642.2 0 333 254 318 642.2 0 318 255 282 642.2 0 282 256 205 642.2 0 205 257 322 642.2 0 322 258 774 642.2 0 774 259 8940 642.2 0 8940 260 452 642.2 0 452 261 171 642.2 0 171 262 207 642.2 0 207 263 532 642.2 0 532 264 412 642.2 0 412 265 281 642.2 0 281 266 362 642.2 0 362 267 98 642.2 0 98 268 168 642.2 0 168 269 104 642.2 0 104 270 189 642.2 0 189 271 157 642.2 0 157 272 137 642.2 0 137 273 125 642.2 0 125 274 578 642.2 0 578 275 222 642.2 0 222 276 267 642.2 0 267 277 318 642.2 0 318 278 509 642.2 0 509 279 127 642.2 0 127 280 106 642.2 0 106 281 313 642.2 0 313 282 113 642.2 0 113 283 100 642.2 0 100 284 144 642.2 0 144 285 161 642.2 0 161 286 128 642.2 0 128 287 114 642.2 0 114 288 106 642.2 0 106 289 277 642.2 0 277 290 138 642.2 0 138 291 104 642.2 0 104 292 131 642.2 0 131 293 145 642.2 0 145 294 347 642.2 0 347 295 113 642.2 0 113 296 80 642.2 0 80 297 140 642.2 0 140 298 108 642.2 0 108 299 165 642.2 0 165 300 125 642.2 0 125 301 326 642.2 0 326 Finished in 2.23 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 58,034 Reads with adapters: 7,708 (13.3%) Reads that were too short: 52 (0.1%) Reads written (passing filters): 7,656 (13.2%) Total basepairs processed: 11,692,031 bp Total written (filtered): 2,095,395 bp (17.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7708 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 35 0.0 3 0 34 1 17 7531 0.0 3 7412 111 4 4 18 18 0.0 3 3 14 1 19 4 0.0 3 0 0 4 20 3 0.0 3 0 0 0 3 22 1 0.0 3 1 24 4 0.0 3 3 0 0 1 26 1 0.0 3 1 27 2 0.0 3 0 0 1 1 29 6 0.0 3 1 0 1 4 30 5 0.0 3 5 31 4 0.0 3 3 1 32 5 0.0 3 3 1 0 1 36 8 0.0 3 0 0 0 8 39 2 0.0 3 0 0 0 2 42 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 54 1 0.0 3 1 57 17 0.0 3 0 0 0 17 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 1 1 66 1 0.0 3 0 0 1 75 2 0.0 3 0 0 0 2 77 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 86 1 0.0 3 1 92 1 0.0 3 0 0 0 1 93 4 0.0 3 0 0 0 4 94 2 0.0 3 1 0 0 1 103 7 0.0 3 0 0 1 6 104 1 0.0 3 0 0 0 1 106 5 0.0 3 0 0 2 3 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 119 2 0.0 3 0 1 0 1 120 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 160 5 0.0 3 0 0 0 5 163 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 178 2 0.0 3 1 1 180 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 7,656 Reads with adapters: 13 (0.2%) Reads written (passing filters): 7,656 (100.0%) Total basepairs processed: 2,095,395 bp Total written (filtered): 2,095,354 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.4% C: 30.8% G: 46.2% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 119.6 0 11 4 2 29.9 0 2