This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.97 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 239,245 (9.1%) Reads that were too short: 25,191 (1.0%) Reads written (passing filters): 214,054 (8.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 58,629,318 bp (7.4%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 239245 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 795 642.2 0 795 7 19288 642.2 0 19288 8 973 642.2 0 973 9 16856 642.2 0 16856 10 763 642.2 0 763 11 1513 642.2 0 1513 12 16164 642.2 0 16164 13 112641 642.2 0 112641 14 16736 642.2 0 16736 15 88 642.2 0 88 16 63 642.2 0 63 17 75 642.2 0 75 18 73 642.2 0 73 19 40 642.2 0 40 20 44 642.2 0 44 21 83 642.2 0 83 22 80 642.2 0 80 23 57 642.2 0 57 24 69 642.2 0 69 25 69 642.2 0 69 26 98 642.2 0 98 27 237 642.2 0 237 28 95 642.2 0 95 29 63 642.2 0 63 30 69 642.2 0 69 31 63 642.2 0 63 32 53 642.2 0 53 33 62 642.2 0 62 34 62 642.2 0 62 35 65 642.2 0 65 36 57 642.2 0 57 37 51 642.2 0 51 38 122 642.2 0 122 39 66 642.2 0 66 40 120 642.2 0 120 41 64 642.2 0 64 42 76 642.2 0 76 43 187 642.2 0 187 44 208 642.2 0 208 45 115 642.2 0 115 46 53 642.2 0 53 47 48 642.2 0 48 48 73 642.2 0 73 49 83 642.2 0 83 50 52 642.2 0 52 51 55 642.2 0 55 52 45 642.2 0 45 53 75 642.2 0 75 54 45 642.2 0 45 55 51 642.2 0 51 56 60 642.2 0 60 57 80 642.2 0 80 58 78 642.2 0 78 59 59 642.2 0 59 60 52 642.2 0 52 61 58 642.2 0 58 62 41 642.2 0 41 63 68 642.2 0 68 64 50 642.2 0 50 65 78 642.2 0 78 66 80 642.2 0 80 67 102 642.2 0 102 68 57 642.2 0 57 69 58 642.2 0 58 70 64 642.2 0 64 71 54 642.2 0 54 72 51 642.2 0 51 73 75 642.2 0 75 74 71 642.2 0 71 75 62 642.2 0 62 76 61 642.2 0 61 77 64 642.2 0 64 78 87 642.2 0 87 79 69 642.2 0 69 80 72 642.2 0 72 81 61 642.2 0 61 82 52 642.2 0 52 83 68 642.2 0 68 84 73 642.2 0 73 85 79 642.2 0 79 86 69 642.2 0 69 87 73 642.2 0 73 88 52 642.2 0 52 89 94 642.2 0 94 90 326 642.2 0 326 91 91 642.2 0 91 92 154 642.2 0 154 93 79 642.2 0 79 94 75 642.2 0 75 95 75 642.2 0 75 96 62 642.2 0 62 97 77 642.2 0 77 98 76 642.2 0 76 99 57 642.2 0 57 100 92 642.2 0 92 101 67 642.2 0 67 102 94 642.2 0 94 103 71 642.2 0 71 104 411 642.2 0 411 105 177 642.2 0 177 106 660 642.2 0 660 107 119 642.2 0 119 108 198 642.2 0 198 109 110 642.2 0 110 110 149 642.2 0 149 111 110 642.2 0 110 112 128 642.2 0 128 113 99 642.2 0 99 114 128 642.2 0 128 115 138 642.2 0 138 116 94 642.2 0 94 117 86 642.2 0 86 118 79 642.2 0 79 119 53 642.2 0 53 120 83 642.2 0 83 121 79 642.2 0 79 122 67 642.2 0 67 123 75 642.2 0 75 124 122 642.2 0 122 125 108 642.2 0 108 126 325 642.2 0 325 127 754 642.2 0 754 128 449 642.2 0 449 129 239 642.2 0 239 130 80 642.2 0 80 131 68 642.2 0 68 132 65 642.2 0 65 133 119 642.2 0 119 134 218 642.2 0 218 135 150 642.2 0 150 136 91 642.2 0 91 137 128 642.2 0 128 138 676 642.2 0 676 139 854 642.2 0 854 140 1680 642.2 0 1680 141 591 642.2 0 591 142 209 642.2 0 209 143 99 642.2 0 99 144 84 642.2 0 84 145 112 642.2 0 112 146 110 642.2 0 110 147 102 642.2 0 102 148 79 642.2 0 79 149 76 642.2 0 76 150 76 642.2 0 76 151 304 642.2 0 304 152 251 642.2 0 251 153 398 642.2 0 398 154 212 642.2 0 212 155 147 642.2 0 147 156 113 642.2 0 113 157 112 642.2 0 112 158 302 642.2 0 302 159 558 642.2 0 558 160 2494 642.2 0 2494 161 988 642.2 0 988 162 213 642.2 0 213 163 528 642.2 0 528 164 101 642.2 0 101 165 77 642.2 0 77 166 70 642.2 0 70 167 117 642.2 0 117 168 123 642.2 0 123 169 170 642.2 0 170 170 83 642.2 0 83 171 103 642.2 0 103 172 87 642.2 0 87 173 80 642.2 0 80 174 136 642.2 0 136 175 116 642.2 0 116 176 118 642.2 0 118 177 100 642.2 0 100 178 78 642.2 0 78 179 75 642.2 0 75 180 84 642.2 0 84 181 148 642.2 0 148 182 296 642.2 0 296 183 139 642.2 0 139 184 114 642.2 0 114 185 91 642.2 0 91 186 143 642.2 0 143 187 88 642.2 0 88 188 193 642.2 0 193 189 129 642.2 0 129 190 146 642.2 0 146 191 51 642.2 0 51 192 79 642.2 0 79 193 61 642.2 0 61 194 73 642.2 0 73 195 68 642.2 0 68 196 77 642.2 0 77 197 82 642.2 0 82 198 69 642.2 0 69 199 108 642.2 0 108 200 97 642.2 0 97 201 106 642.2 0 106 202 98 642.2 0 98 203 87 642.2 0 87 204 73 642.2 0 73 205 91 642.2 0 91 206 70 642.2 0 70 207 102 642.2 0 102 208 98 642.2 0 98 209 103 642.2 0 103 210 60 642.2 0 60 211 96 642.2 0 96 212 81 642.2 0 81 213 142 642.2 0 142 214 272 642.2 0 272 215 228 642.2 0 228 216 163 642.2 0 163 217 120 642.2 0 120 218 119 642.2 0 119 219 96 642.2 0 96 220 291 642.2 0 291 221 442 642.2 0 442 222 299 642.2 0 299 223 150 642.2 0 150 224 225 642.2 0 225 225 132 642.2 0 132 226 319 642.2 0 319 227 81 642.2 0 81 228 75 642.2 0 75 229 73 642.2 0 73 230 106 642.2 0 106 231 147 642.2 0 147 232 192 642.2 0 192 233 483 642.2 0 483 234 568 642.2 0 568 235 197 642.2 0 197 236 198 642.2 0 198 237 82 642.2 0 82 238 77 642.2 0 77 239 71 642.2 0 71 240 131 642.2 0 131 241 185 642.2 0 185 242 190 642.2 0 190 243 143 642.2 0 143 244 117 642.2 0 117 245 64 642.2 0 64 246 59 642.2 0 59 247 99 642.2 0 99 248 103 642.2 0 103 249 63 642.2 0 63 250 96 642.2 0 96 251 91 642.2 0 91 252 95 642.2 0 95 253 154 642.2 0 154 254 129 642.2 0 129 255 82 642.2 0 82 256 77 642.2 0 77 257 101 642.2 0 101 258 87 642.2 0 87 259 75 642.2 0 75 260 129 642.2 0 129 261 129 642.2 0 129 262 146 642.2 0 146 263 117 642.2 0 117 264 96 642.2 0 96 265 89 642.2 0 89 266 98 642.2 0 98 267 225 642.2 0 225 268 211 642.2 0 211 269 348 642.2 0 348 270 149 642.2 0 149 271 100 642.2 0 100 272 100 642.2 0 100 273 102 642.2 0 102 274 88 642.2 0 88 275 105 642.2 0 105 276 134 642.2 0 134 277 452 642.2 0 452 278 382 642.2 0 382 279 352 642.2 0 352 280 711 642.2 0 711 281 2993 642.2 0 2993 282 3188 642.2 0 3188 283 2551 642.2 0 2551 284 737 642.2 0 737 285 112 642.2 0 112 286 160 642.2 0 160 287 168 642.2 0 168 288 488 642.2 0 488 289 221 642.2 0 221 290 661 642.2 0 661 291 176 642.2 0 176 292 107 642.2 0 107 293 91 642.2 0 91 294 130 642.2 0 130 295 147 642.2 0 147 296 137 642.2 0 137 297 77 642.2 0 77 298 94 642.2 0 94 299 72 642.2 0 72 300 69 642.2 0 69 301 101 642.2 0 101 Finished in 10.73 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 214,054 Reads with adapters: 19,216 (9.0%) Reads that were too short: 134 (0.1%) Reads written (passing filters): 19,082 (8.9%) Total basepairs processed: 58,629,318 bp Total written (filtered): 4,967,927 bp (8.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19216 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 8 0.0 3 0 0 2 5 1 19 79 0.0 3 0 43 19 13 4 20 16692 0.0 4 15270 1093 223 86 20 21 44 0.0 4 4 30 6 1 3 22 25 0.0 4 1 1 3 0 20 23 109 0.0 4 19 0 0 49 41 24 1 0.0 4 0 0 0 1 29 1 0.0 4 1 30 18 0.0 4 0 0 0 0 18 32 2 0.0 4 0 0 0 0 2 36 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 42 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 54 1 0.0 4 1 65 1 0.0 4 0 0 0 0 1 69 9 0.0 4 0 0 0 0 9 78 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 87 4 0.0 4 0 0 0 0 4 88 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 6 0.0 4 0 0 0 0 6 110 2 0.0 4 0 0 0 0 2 111 1 0.0 4 0 0 0 0 1 118 2 0.0 4 0 1 0 0 1 119 1 0.0 4 0 0 0 1 120 3 0.0 4 0 0 0 0 3 121 15 0.0 4 0 0 0 0 15 122 1745 0.0 4 0 0 1 0 1744 123 8 0.0 4 0 0 0 0 8 124 2 0.0 4 0 0 0 0 2 125 6 0.0 4 0 0 0 0 6 126 3 0.0 4 0 0 0 0 3 127 249 0.0 4 0 0 0 0 249 128 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 1 135 1 0.0 4 0 1 137 1 0.0 4 1 138 1 0.0 4 1 142 1 0.0 4 0 1 143 1 0.0 4 0 0 0 0 1 145 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 148 20 0.0 4 0 0 0 0 20 149 1 0.0 4 0 0 0 0 1 151 2 0.0 4 0 1 0 0 1 155 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 1 159 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 1 183 1 0.0 4 1 186 1 0.0 4 1 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 1 198 1 0.0 4 0 0 0 1 202 3 0.0 4 0 0 0 0 3 203 5 0.0 4 0 0 0 0 5 205 1 0.0 4 0 0 1 208 2 0.0 4 0 0 0 0 2 209 2 0.0 4 0 0 0 0 2 210 4 0.0 4 0 0 0 0 4 211 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 220 3 0.0 4 0 0 0 1 2 221 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 1 223 9 0.0 4 0 0 0 1 8 224 6 0.0 4 0 0 0 0 6 225 2 0.0 4 0 0 0 1 1 226 2 0.0 4 0 0 0 0 2 227 3 0.0 4 0 0 0 1 2 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 0 0 0 3 232 4 0.0 4 0 0 0 0 4 233 1 0.0 4 0 1 237 1 0.0 4 0 0 0 1 238 1 0.0 4 0 0 1 243 1 0.0 4 0 0 0 0 1 244 2 0.0 4 0 1 0 0 1 246 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 252 2 0.0 4 0 0 0 0 2 257 2 0.0 4 0 0 0 0 2 258 4 0.0 4 0 0 0 0 4 259 4 0.0 4 0 0 0 0 4 260 2 0.0 4 0 0 0 1 1 264 3 0.0 4 0 0 0 0 3 266 1 0.0 4 0 0 0 1 277 4 0.0 4 0 0 0 2 2 278 2 0.0 4 0 0 0 0 2 279 1 0.0 4 0 1 280 4 0.0 4 0 0 0 0 4 282 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 1 1 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 287 2 0.0 4 0 0 0 0 2 288 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.68 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 19,082 Reads with adapters: 4,919 (25.8%) Reads written (passing filters): 19,082 (100.0%) Total basepairs processed: 4,967,927 bp Total written (filtered): 4,911,854 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4919 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 9.8% G: 85.4% T: 2.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 298.2 0 16 4 24 74.5 0 24 7 2 1.2 0 2 9 1885 0.1 0 223 1662 10 2714 0.0 1 233 2481 11 193 0.0 1 8 185 12 14 0.0 1 0 14 13 9 0.0 1 0 9 44 1 0.0 1 1 56 1 0.0 1 0 1 62 1 0.0 1 0 1 70 1 0.0 1 1 72 2 0.0 1 1 1 80 10 0.0 1 6 4 114 17 0.0 1 15 2 122 2 0.0 1 1 1 126 7 0.0 1 6 1 201 1 0.0 1 0 1 208 1 0.0 1 1 223 1 0.0 1 1 265 1 0.0 1 1 266 7 0.0 1 5 2 267 9 0.0 1 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.