This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.86 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 209,699 (8.0%) Reads that were too short: 15,690 (0.6%) Reads written (passing filters): 194,009 (7.4%) Total basepairs processed: 791,719,999 bp Total written (filtered): 54,247,535 bp (6.9%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 209699 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 723 642.2 0 723 7 18300 642.2 0 18300 8 704 642.2 0 704 9 19738 642.2 0 19738 10 610 642.2 0 610 11 1297 642.2 0 1297 12 14481 642.2 0 14481 13 105716 642.2 0 105716 14 15781 642.2 0 15781 15 64 642.2 0 64 16 46 642.2 0 46 17 47 642.2 0 47 18 34 642.2 0 34 19 30 642.2 0 30 20 40 642.2 0 40 21 27 642.2 0 27 22 43 642.2 0 43 23 42 642.2 0 42 24 50 642.2 0 50 25 40 642.2 0 40 26 38 642.2 0 38 27 41 642.2 0 41 28 47 642.2 0 47 29 54 642.2 0 54 30 47 642.2 0 47 31 46 642.2 0 46 32 43 642.2 0 43 33 35 642.2 0 35 34 28 642.2 0 28 35 36 642.2 0 36 36 40 642.2 0 40 37 37 642.2 0 37 38 116 642.2 0 116 39 34 642.2 0 34 40 84 642.2 0 84 41 57 642.2 0 57 42 46 642.2 0 46 43 120 642.2 0 120 44 103 642.2 0 103 45 81 642.2 0 81 46 48 642.2 0 48 47 31 642.2 0 31 48 49 642.2 0 49 49 54 642.2 0 54 50 67 642.2 0 67 51 43 642.2 0 43 52 33 642.2 0 33 53 58 642.2 0 58 54 45 642.2 0 45 55 49 642.2 0 49 56 38 642.2 0 38 57 53 642.2 0 53 58 51 642.2 0 51 59 50 642.2 0 50 60 42 642.2 0 42 61 51 642.2 0 51 62 43 642.2 0 43 63 45 642.2 0 45 64 51 642.2 0 51 65 51 642.2 0 51 66 54 642.2 0 54 67 60 642.2 0 60 68 40 642.2 0 40 69 39 642.2 0 39 70 65 642.2 0 65 71 38 642.2 0 38 72 31 642.2 0 31 73 53 642.2 0 53 74 48 642.2 0 48 75 38 642.2 0 38 76 49 642.2 0 49 77 66 642.2 0 66 78 70 642.2 0 70 79 65 642.2 0 65 80 75 642.2 0 75 81 49 642.2 0 49 82 55 642.2 0 55 83 60 642.2 0 60 84 46 642.2 0 46 85 48 642.2 0 48 86 76 642.2 0 76 87 66 642.2 0 66 88 66 642.2 0 66 89 79 642.2 0 79 90 305 642.2 0 305 91 84 642.2 0 84 92 115 642.2 0 115 93 53 642.2 0 53 94 76 642.2 0 76 95 52 642.2 0 52 96 62 642.2 0 62 97 66 642.2 0 66 98 81 642.2 0 81 99 45 642.2 0 45 100 54 642.2 0 54 101 46 642.2 0 46 102 87 642.2 0 87 103 78 642.2 0 78 104 183 642.2 0 183 105 97 642.2 0 97 106 222 642.2 0 222 107 78 642.2 0 78 108 87 642.2 0 87 109 67 642.2 0 67 110 89 642.2 0 89 111 90 642.2 0 90 112 90 642.2 0 90 113 75 642.2 0 75 114 71 642.2 0 71 115 99 642.2 0 99 116 93 642.2 0 93 117 64 642.2 0 64 118 64 642.2 0 64 119 58 642.2 0 58 120 61 642.2 0 61 121 71 642.2 0 71 122 85 642.2 0 85 123 53 642.2 0 53 124 81 642.2 0 81 125 63 642.2 0 63 126 244 642.2 0 244 127 692 642.2 0 692 128 322 642.2 0 322 129 136 642.2 0 136 130 68 642.2 0 68 131 56 642.2 0 56 132 62 642.2 0 62 133 64 642.2 0 64 134 153 642.2 0 153 135 118 642.2 0 118 136 62 642.2 0 62 137 70 642.2 0 70 138 125 642.2 0 125 139 232 642.2 0 232 140 406 642.2 0 406 141 319 642.2 0 319 142 163 642.2 0 163 143 57 642.2 0 57 144 73 642.2 0 73 145 58 642.2 0 58 146 83 642.2 0 83 147 75 642.2 0 75 148 62 642.2 0 62 149 52 642.2 0 52 150 71 642.2 0 71 151 191 642.2 0 191 152 148 642.2 0 148 153 275 642.2 0 275 154 170 642.2 0 170 155 79 642.2 0 79 156 77 642.2 0 77 157 72 642.2 0 72 158 123 642.2 0 123 159 254 642.2 0 254 160 908 642.2 0 908 161 368 642.2 0 368 162 139 642.2 0 139 163 180 642.2 0 180 164 81 642.2 0 81 165 70 642.2 0 70 166 46 642.2 0 46 167 67 642.2 0 67 168 86 642.2 0 86 169 169 642.2 0 169 170 61 642.2 0 61 171 83 642.2 0 83 172 47 642.2 0 47 173 52 642.2 0 52 174 83 642.2 0 83 175 68 642.2 0 68 176 72 642.2 0 72 177 53 642.2 0 53 178 68 642.2 0 68 179 74 642.2 0 74 180 64 642.2 0 64 181 89 642.2 0 89 182 131 642.2 0 131 183 58 642.2 0 58 184 75 642.2 0 75 185 64 642.2 0 64 186 169 642.2 0 169 187 73 642.2 0 73 188 91 642.2 0 91 189 82 642.2 0 82 190 80 642.2 0 80 191 49 642.2 0 49 192 59 642.2 0 59 193 70 642.2 0 70 194 63 642.2 0 63 195 44 642.2 0 44 196 54 642.2 0 54 197 63 642.2 0 63 198 63 642.2 0 63 199 75 642.2 0 75 200 59 642.2 0 59 201 68 642.2 0 68 202 87 642.2 0 87 203 81 642.2 0 81 204 63 642.2 0 63 205 60 642.2 0 60 206 64 642.2 0 64 207 61 642.2 0 61 208 77 642.2 0 77 209 92 642.2 0 92 210 53 642.2 0 53 211 60 642.2 0 60 212 45 642.2 0 45 213 67 642.2 0 67 214 150 642.2 0 150 215 130 642.2 0 130 216 89 642.2 0 89 217 77 642.2 0 77 218 57 642.2 0 57 219 61 642.2 0 61 220 137 642.2 0 137 221 213 642.2 0 213 222 170 642.2 0 170 223 123 642.2 0 123 224 242 642.2 0 242 225 96 642.2 0 96 226 326 642.2 0 326 227 78 642.2 0 78 228 59 642.2 0 59 229 60 642.2 0 60 230 83 642.2 0 83 231 88 642.2 0 88 232 146 642.2 0 146 233 258 642.2 0 258 234 726 642.2 0 726 235 185 642.2 0 185 236 176 642.2 0 176 237 87 642.2 0 87 238 55 642.2 0 55 239 55 642.2 0 55 240 64 642.2 0 64 241 91 642.2 0 91 242 99 642.2 0 99 243 69 642.2 0 69 244 54 642.2 0 54 245 62 642.2 0 62 246 52 642.2 0 52 247 52 642.2 0 52 248 74 642.2 0 74 249 47 642.2 0 47 250 81 642.2 0 81 251 72 642.2 0 72 252 78 642.2 0 78 253 102 642.2 0 102 254 60 642.2 0 60 255 57 642.2 0 57 256 56 642.2 0 56 257 88 642.2 0 88 258 62 642.2 0 62 259 41 642.2 0 41 260 77 642.2 0 77 261 91 642.2 0 91 262 86 642.2 0 86 263 81 642.2 0 81 264 69 642.2 0 69 265 69 642.2 0 69 266 66 642.2 0 66 267 110 642.2 0 110 268 129 642.2 0 129 269 187 642.2 0 187 270 74 642.2 0 74 271 68 642.2 0 68 272 79 642.2 0 79 273 69 642.2 0 69 274 87 642.2 0 87 275 95 642.2 0 95 276 107 642.2 0 107 277 491 642.2 0 491 278 294 642.2 0 294 279 295 642.2 0 295 280 319 642.2 0 319 281 1274 642.2 0 1274 282 1712 642.2 0 1712 283 1024 642.2 0 1024 284 323 642.2 0 323 285 65 642.2 0 65 286 101 642.2 0 101 287 123 642.2 0 123 288 286 642.2 0 286 289 148 642.2 0 148 290 624 642.2 0 624 291 111 642.2 0 111 292 107 642.2 0 107 293 71 642.2 0 71 294 98 642.2 0 98 295 132 642.2 0 132 296 113 642.2 0 113 297 84 642.2 0 84 298 48 642.2 0 48 299 60 642.2 0 60 300 62 642.2 0 62 301 83 642.2 0 83 Finished in 8.64 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 194,009 Reads with adapters: 19,563 (10.1%) Reads that were too short: 201 (0.1%) Reads written (passing filters): 19,362 (10.0%) Total basepairs processed: 54,247,535 bp Total written (filtered): 5,164,141 bp (9.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19563 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 64 0.0 3 0 60 4 17 17706 0.0 3 17374 326 5 1 18 30 0.0 3 1 28 1 19 9 0.0 3 1 0 7 1 20 97 0.0 3 92 1 0 4 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 1 25 2 0.0 3 2 26 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 31 23 0.0 3 0 1 4 18 32 9 0.0 3 1 0 0 8 33 2 0.0 3 0 0 0 2 34 1 0.0 3 1 37 3 0.0 3 0 0 0 3 39 6 0.0 3 0 0 0 6 42 6 0.0 3 0 0 0 6 43 5 0.0 3 0 0 0 5 44 1 0.0 3 0 0 0 1 45 4 0.0 3 0 0 0 4 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 52 1 0.0 3 1 53 4 0.0 3 1 0 0 3 54 3 0.0 3 1 0 0 2 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 13 0.0 3 0 0 0 13 58 1 0.0 3 0 0 0 1 59 12 0.0 3 0 0 0 12 61 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 6 0.0 3 0 0 0 6 66 2 0.0 3 0 0 0 2 67 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 72 2 0.0 3 0 0 0 2 73 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 1 0 0 1 79 1 0.0 3 1 80 3 0.0 3 0 0 0 3 81 3 0.0 3 0 0 0 3 84 51 0.0 3 0 0 0 51 85 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 91 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 98 2 0.0 3 0 0 0 2 99 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 101 139 0.0 3 0 0 123 16 105 2 0.0 3 0 0 0 2 107 2 0.0 3 0 0 0 2 108 4 0.0 3 0 0 0 4 109 3 0.0 3 0 0 0 3 110 35 0.0 3 0 0 0 35 111 3 0.0 3 0 0 1 2 112 1 0.0 3 0 0 0 1 118 12 0.0 3 0 0 0 12 119 1 0.0 3 0 0 0 1 120 8 0.0 3 0 0 0 8 122 5 0.0 3 0 0 0 5 124 3 0.0 3 0 1 1 1 126 1 0.0 3 0 0 0 1 127 5 0.0 3 0 0 0 5 129 26 0.0 3 0 0 0 26 130 4 0.0 3 3 0 1 133 5 0.0 3 0 0 0 5 135 3 0.0 3 0 0 0 3 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 1 0.0 3 1 140 1 0.0 3 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 1 146 8 0.0 3 0 0 0 8 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 151 6 0.0 3 1 0 0 5 152 5 0.0 3 0 0 0 5 154 7 0.0 3 0 0 0 7 155 2 0.0 3 0 0 0 2 156 4 0.0 3 0 0 0 4 157 3 0.0 3 0 0 0 3 158 5 0.0 3 0 0 0 5 159 118 0.0 3 0 0 2 116 160 22 0.0 3 0 0 0 22 161 43 0.0 3 1 0 2 40 162 13 0.0 3 0 0 1 12 163 6 0.0 3 0 0 0 6 164 4 0.0 3 0 0 0 4 166 4 0.0 3 0 0 0 4 167 1 0.0 3 0 0 0 1 168 3 0.0 3 0 1 0 2 169 2 0.0 3 0 0 0 2 170 3 0.0 3 0 0 0 3 171 3 0.0 3 0 0 0 3 174 4 0.0 3 0 0 0 4 176 4 0.0 3 0 0 0 4 177 4 0.0 3 0 0 0 4 178 8 0.0 3 0 0 0 8 179 413 0.0 3 0 0 1 412 180 160 0.0 3 0 0 1 159 181 8 0.0 3 0 0 1 7 182 31 0.0 3 0 0 2 29 183 6 0.0 3 0 0 0 6 184 1 0.0 3 0 0 0 1 185 4 0.0 3 0 0 0 4 187 2 0.0 3 0 0 0 2 188 67 0.0 3 0 0 0 67 190 2 0.0 3 0 1 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 196 7 0.0 3 0 0 0 7 198 1 0.0 3 0 0 0 1 201 1 0.0 3 1 202 2 0.0 3 2 203 3 0.0 3 2 0 0 1 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 3 0.0 3 0 0 0 3 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 218 3 0.0 3 2 0 1 221 2 0.0 3 0 0 0 2 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 10 0.0 3 0 0 0 10 231 1 0.0 3 0 0 0 1 233 2 0.0 3 1 0 0 1 234 2 0.0 3 0 0 0 2 237 17 0.0 3 0 0 0 17 239 6 0.0 3 0 0 0 6 240 2 0.0 3 0 0 0 2 242 2 0.0 3 0 0 0 2 246 4 0.0 3 0 0 1 3 248 1 0.0 3 0 0 0 1 252 2 0.0 3 1 0 0 1 254 3 0.0 3 0 0 0 3 261 1 0.0 3 0 0 0 1 263 13 0.0 3 0 0 1 12 264 1 0.0 3 0 0 0 1 266 3 0.0 3 0 0 0 3 267 3 0.0 3 0 0 0 3 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 271 10 0.0 3 0 0 0 10 272 3 0.0 3 0 0 0 3 273 5 0.0 3 0 0 0 5 274 7 0.0 3 0 0 0 7 275 9 0.0 3 2 0 0 7 276 8 0.0 3 0 1 0 7 278 2 0.0 3 1 0 0 1 279 1 0.0 3 0 0 0 1 280 13 0.0 3 0 0 0 13 281 6 0.0 3 0 0 0 6 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 285 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.65 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 19,362 Reads with adapters: 69 (0.4%) Reads written (passing filters): 19,362 (100.0%) Total basepairs processed: 5,164,141 bp Total written (filtered): 5,163,929 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 69 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.7% C: 21.7% G: 20.3% T: 7.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 65 302.5 0 65 4 3 75.6 0 3 5 1 18.9 0 1