This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.94 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 94,289 (3.6%) Reads that were too short: 19,159 (0.7%) Reads written (passing filters): 75,130 (2.9%) Total basepairs processed: 791,719,999 bp Total written (filtered): 19,308,870 bp (2.4%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 94289 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 990 642.2 0 990 7 22675 642.2 0 22675 8 381 642.2 0 381 9 26887 642.2 0 26887 10 92 642.2 0 92 11 79 642.2 0 79 12 58 642.2 0 58 13 71 642.2 0 71 14 67 642.2 0 67 15 60 642.2 0 60 16 68 642.2 0 68 17 79 642.2 0 79 18 44 642.2 0 44 19 64 642.2 0 64 20 78 642.2 0 78 21 93 642.2 0 93 22 91 642.2 0 91 23 82 642.2 0 82 24 76 642.2 0 76 25 65 642.2 0 65 26 69 642.2 0 69 27 61 642.2 0 61 28 42 642.2 0 42 29 105 642.2 0 105 30 86 642.2 0 86 31 70 642.2 0 70 32 56 642.2 0 56 33 54 642.2 0 54 34 58 642.2 0 58 35 63 642.2 0 63 36 65 642.2 0 65 37 84 642.2 0 84 38 62 642.2 0 62 39 84 642.2 0 84 40 79 642.2 0 79 41 71 642.2 0 71 42 60 642.2 0 60 43 61 642.2 0 61 44 56 642.2 0 56 45 65 642.2 0 65 46 80 642.2 0 80 47 59 642.2 0 59 48 62 642.2 0 62 49 86 642.2 0 86 50 61 642.2 0 61 51 69 642.2 0 69 52 83 642.2 0 83 53 109 642.2 0 109 54 77 642.2 0 77 55 111 642.2 0 111 56 68 642.2 0 68 57 52 642.2 0 52 58 95 642.2 0 95 59 65 642.2 0 65 60 68 642.2 0 68 61 58 642.2 0 58 62 76 642.2 0 76 63 65 642.2 0 65 64 103 642.2 0 103 65 80 642.2 0 80 66 47 642.2 0 47 67 85 642.2 0 85 68 82 642.2 0 82 69 84 642.2 0 84 70 97 642.2 0 97 71 216 642.2 0 216 72 86 642.2 0 86 73 154 642.2 0 154 74 134 642.2 0 134 75 171 642.2 0 171 76 80 642.2 0 80 77 69 642.2 0 69 78 63 642.2 0 63 79 67 642.2 0 67 80 89 642.2 0 89 81 88 642.2 0 88 82 70 642.2 0 70 83 78 642.2 0 78 84 89 642.2 0 89 85 76 642.2 0 76 86 84 642.2 0 84 87 79 642.2 0 79 88 80 642.2 0 80 89 95 642.2 0 95 90 98 642.2 0 98 91 82 642.2 0 82 92 60 642.2 0 60 93 74 642.2 0 74 94 83 642.2 0 83 95 78 642.2 0 78 96 97 642.2 0 97 97 76 642.2 0 76 98 87 642.2 0 87 99 105 642.2 0 105 100 112 642.2 0 112 101 657 642.2 0 657 102 482 642.2 0 482 103 729 642.2 0 729 104 101 642.2 0 101 105 86 642.2 0 86 106 89 642.2 0 89 107 50 642.2 0 50 108 85 642.2 0 85 109 78 642.2 0 78 110 117 642.2 0 117 111 417 642.2 0 417 112 341 642.2 0 341 113 343 642.2 0 343 114 124 642.2 0 124 115 119 642.2 0 119 116 99 642.2 0 99 117 110 642.2 0 110 118 94 642.2 0 94 119 72 642.2 0 72 120 73 642.2 0 73 121 80 642.2 0 80 122 80 642.2 0 80 123 150 642.2 0 150 124 163 642.2 0 163 125 132 642.2 0 132 126 100 642.2 0 100 127 91 642.2 0 91 128 88 642.2 0 88 129 90 642.2 0 90 130 103 642.2 0 103 131 133 642.2 0 133 132 330 642.2 0 330 133 185 642.2 0 185 134 241 642.2 0 241 135 82 642.2 0 82 136 117 642.2 0 117 137 174 642.2 0 174 138 242 642.2 0 242 139 197 642.2 0 197 140 122 642.2 0 122 141 197 642.2 0 197 142 188 642.2 0 188 143 181 642.2 0 181 144 392 642.2 0 392 145 384 642.2 0 384 146 347 642.2 0 347 147 79 642.2 0 79 148 136 642.2 0 136 149 160 642.2 0 160 150 192 642.2 0 192 151 149 642.2 0 149 152 104 642.2 0 104 153 84 642.2 0 84 154 181 642.2 0 181 155 152 642.2 0 152 156 186 642.2 0 186 157 142 642.2 0 142 158 135 642.2 0 135 159 143 642.2 0 143 160 121 642.2 0 121 161 98 642.2 0 98 162 119 642.2 0 119 163 161 642.2 0 161 164 142 642.2 0 142 165 117 642.2 0 117 166 194 642.2 0 194 167 102 642.2 0 102 168 144 642.2 0 144 169 214 642.2 0 214 170 499 642.2 0 499 171 353 642.2 0 353 172 322 642.2 0 322 173 102 642.2 0 102 174 58 642.2 0 58 175 79 642.2 0 79 176 85 642.2 0 85 177 199 642.2 0 199 178 288 642.2 0 288 179 208 642.2 0 208 180 523 642.2 0 523 181 94 642.2 0 94 182 78 642.2 0 78 183 111 642.2 0 111 184 115 642.2 0 115 185 126 642.2 0 126 186 98 642.2 0 98 187 102 642.2 0 102 188 97 642.2 0 97 189 129 642.2 0 129 190 102 642.2 0 102 191 74 642.2 0 74 192 81 642.2 0 81 193 70 642.2 0 70 194 94 642.2 0 94 195 100 642.2 0 100 196 86 642.2 0 86 197 103 642.2 0 103 198 105 642.2 0 105 199 137 642.2 0 137 200 112 642.2 0 112 201 97 642.2 0 97 202 170 642.2 0 170 203 145 642.2 0 145 204 198 642.2 0 198 205 219 642.2 0 219 206 139 642.2 0 139 207 84 642.2 0 84 208 74 642.2 0 74 209 144 642.2 0 144 210 204 642.2 0 204 211 172 642.2 0 172 212 116 642.2 0 116 213 98 642.2 0 98 214 105 642.2 0 105 215 94 642.2 0 94 216 115 642.2 0 115 217 134 642.2 0 134 218 137 642.2 0 137 219 120 642.2 0 120 220 118 642.2 0 118 221 83 642.2 0 83 222 103 642.2 0 103 223 166 642.2 0 166 224 187 642.2 0 187 225 216 642.2 0 216 226 202 642.2 0 202 227 213 642.2 0 213 228 152 642.2 0 152 229 127 642.2 0 127 230 102 642.2 0 102 231 106 642.2 0 106 232 101 642.2 0 101 233 116 642.2 0 116 234 112 642.2 0 112 235 106 642.2 0 106 236 122 642.2 0 122 237 101 642.2 0 101 238 102 642.2 0 102 239 88 642.2 0 88 240 103 642.2 0 103 241 100 642.2 0 100 242 102 642.2 0 102 243 151 642.2 0 151 244 216 642.2 0 216 245 304 642.2 0 304 246 270 642.2 0 270 247 316 642.2 0 316 248 195 642.2 0 195 249 141 642.2 0 141 250 94 642.2 0 94 251 85 642.2 0 85 252 80 642.2 0 80 253 123 642.2 0 123 254 102 642.2 0 102 255 100 642.2 0 100 256 204 642.2 0 204 257 245 642.2 0 245 258 214 642.2 0 214 259 223 642.2 0 223 260 201 642.2 0 201 261 130 642.2 0 130 262 223 642.2 0 223 263 155 642.2 0 155 264 302 642.2 0 302 265 335 642.2 0 335 266 380 642.2 0 380 267 189 642.2 0 189 268 147 642.2 0 147 269 117 642.2 0 117 270 130 642.2 0 130 271 174 642.2 0 174 272 248 642.2 0 248 273 234 642.2 0 234 274 183 642.2 0 183 275 131 642.2 0 131 276 82 642.2 0 82 277 102 642.2 0 102 278 110 642.2 0 110 279 137 642.2 0 137 280 151 642.2 0 151 281 195 642.2 0 195 282 161 642.2 0 161 283 197 642.2 0 197 284 961 642.2 0 961 285 1249 642.2 0 1249 286 1191 642.2 0 1191 287 869 642.2 0 869 288 226 642.2 0 226 289 114 642.2 0 114 290 115 642.2 0 115 291 174 642.2 0 174 292 209 642.2 0 209 293 241 642.2 0 241 294 112 642.2 0 112 295 119 642.2 0 119 296 99 642.2 0 99 297 119 642.2 0 119 298 107 642.2 0 107 299 125 642.2 0 125 300 99 642.2 0 99 301 157 642.2 0 157 Finished in 3.68 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 75,130 Reads with adapters: 23,362 (31.1%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 23,331 (31.1%) Total basepairs processed: 19,308,870 bp Total written (filtered): 6,365,557 bp (33.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 23362 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 1 3 18 10 0.0 3 0 0 2 2 6 19 150 0.0 3 0 106 23 14 7 20 22412 0.0 4 20896 1200 224 65 27 21 55 0.0 4 6 45 2 2 22 14 0.0 4 2 3 6 3 23 437 0.0 4 355 33 6 28 15 24 2 0.0 4 0 1 0 0 1 26 3 0.0 4 0 0 0 1 2 27 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 35 1 0.0 4 1 37 2 0.0 4 0 1 0 0 1 51 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 3 0.0 4 0 0 0 0 3 125 5 0.0 4 0 0 0 0 5 126 1 0.0 4 0 0 0 0 1 127 218 0.0 4 0 0 0 0 218 128 4 0.0 4 0 0 0 0 4 137 1 0.0 4 0 0 0 1 153 7 0.0 4 0 0 0 1 6 163 1 0.0 4 0 0 0 1 176 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.82 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 23,331 Reads with adapters: 1,969 (8.4%) Reads written (passing filters): 23,331 (100.0%) Total basepairs processed: 6,365,557 bp Total written (filtered): 6,335,403 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1969 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 43.2% C: 11.0% G: 43.9% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 193 364.5 0 193 4 26 91.1 0 26 8 1 0.4 0 1 9 64 0.1 0 9 55 10 81 0.0 1 11 70 11 475 0.0 1 23 452 12 343 0.0 1 7 336 13 9 0.0 1 0 9 14 1 0.0 1 0 1 15 8 0.0 1 3 5 19 2 0.0 1 1 1 20 683 0.0 1 446 237 21 31 0.0 1 23 8 22 1 0.0 1 1 24 34 0.0 1 29 5 152 1 0.0 1 1 158 1 0.0 1 1 186 5 0.0 1 5 187 1 0.0 1 0 1 191 1 0.0 1 0 1 210 8 0.0 1 7 1