This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.47 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 76,618 (2.9%) Reads that were too short: 9,795 (0.4%) Reads written (passing filters): 66,823 (2.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 18,174,662 bp (2.3%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 76618 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1019 642.2 0 1019 7 30414 642.2 0 30414 8 261 642.2 0 261 9 21975 642.2 0 21975 10 66 642.2 0 66 11 45 642.2 0 45 12 33 642.2 0 33 13 44 642.2 0 44 14 34 642.2 0 34 15 37 642.2 0 37 16 46 642.2 0 46 17 38 642.2 0 38 18 42 642.2 0 42 19 46 642.2 0 46 20 56 642.2 0 56 21 64 642.2 0 64 22 51 642.2 0 51 23 71 642.2 0 71 24 61 642.2 0 61 25 34 642.2 0 34 26 51 642.2 0 51 27 40 642.2 0 40 28 37 642.2 0 37 29 62 642.2 0 62 30 32 642.2 0 32 31 35 642.2 0 35 32 39 642.2 0 39 33 40 642.2 0 40 34 33 642.2 0 33 35 43 642.2 0 43 36 37 642.2 0 37 37 25 642.2 0 25 38 32 642.2 0 32 39 45 642.2 0 45 40 41 642.2 0 41 41 37 642.2 0 37 42 50 642.2 0 50 43 48 642.2 0 48 44 44 642.2 0 44 45 38 642.2 0 38 46 56 642.2 0 56 47 43 642.2 0 43 48 41 642.2 0 41 49 51 642.2 0 51 50 84 642.2 0 84 51 44 642.2 0 44 52 53 642.2 0 53 53 55 642.2 0 55 54 46 642.2 0 46 55 70 642.2 0 70 56 48 642.2 0 48 57 35 642.2 0 35 58 69 642.2 0 69 59 39 642.2 0 39 60 42 642.2 0 42 61 39 642.2 0 39 62 32 642.2 0 32 63 49 642.2 0 49 64 66 642.2 0 66 65 54 642.2 0 54 66 52 642.2 0 52 67 61 642.2 0 61 68 47 642.2 0 47 69 48 642.2 0 48 70 70 642.2 0 70 71 265 642.2 0 265 72 63 642.2 0 63 73 76 642.2 0 76 74 80 642.2 0 80 75 93 642.2 0 93 76 52 642.2 0 52 77 45 642.2 0 45 78 38 642.2 0 38 79 37 642.2 0 37 80 59 642.2 0 59 81 59 642.2 0 59 82 48 642.2 0 48 83 37 642.2 0 37 84 44 642.2 0 44 85 44 642.2 0 44 86 63 642.2 0 63 87 55 642.2 0 55 88 34 642.2 0 34 89 58 642.2 0 58 90 59 642.2 0 59 91 43 642.2 0 43 92 45 642.2 0 45 93 42 642.2 0 42 94 40 642.2 0 40 95 52 642.2 0 52 96 51 642.2 0 51 97 30 642.2 0 30 98 35 642.2 0 35 99 52 642.2 0 52 100 56 642.2 0 56 101 246 642.2 0 246 102 157 642.2 0 157 103 229 642.2 0 229 104 63 642.2 0 63 105 53 642.2 0 53 106 49 642.2 0 49 107 52 642.2 0 52 108 49 642.2 0 49 109 49 642.2 0 49 110 46 642.2 0 46 111 155 642.2 0 155 112 137 642.2 0 137 113 126 642.2 0 126 114 61 642.2 0 61 115 62 642.2 0 62 116 56 642.2 0 56 117 73 642.2 0 73 118 54 642.2 0 54 119 50 642.2 0 50 120 41 642.2 0 41 121 44 642.2 0 44 122 47 642.2 0 47 123 68 642.2 0 68 124 87 642.2 0 87 125 66 642.2 0 66 126 64 642.2 0 64 127 49 642.2 0 49 128 38 642.2 0 38 129 37 642.2 0 37 130 66 642.2 0 66 131 78 642.2 0 78 132 288 642.2 0 288 133 154 642.2 0 154 134 148 642.2 0 148 135 39 642.2 0 39 136 58 642.2 0 58 137 103 642.2 0 103 138 154 642.2 0 154 139 113 642.2 0 113 140 56 642.2 0 56 141 90 642.2 0 90 142 90 642.2 0 90 143 80 642.2 0 80 144 208 642.2 0 208 145 232 642.2 0 232 146 195 642.2 0 195 147 43 642.2 0 43 148 113 642.2 0 113 149 111 642.2 0 111 150 131 642.2 0 131 151 84 642.2 0 84 152 49 642.2 0 49 153 55 642.2 0 55 154 90 642.2 0 90 155 82 642.2 0 82 156 89 642.2 0 89 157 55 642.2 0 55 158 82 642.2 0 82 159 63 642.2 0 63 160 43 642.2 0 43 161 49 642.2 0 49 162 46 642.2 0 46 163 76 642.2 0 76 164 52 642.2 0 52 165 55 642.2 0 55 166 83 642.2 0 83 167 52 642.2 0 52 168 78 642.2 0 78 169 110 642.2 0 110 170 229 642.2 0 229 171 157 642.2 0 157 172 171 642.2 0 171 173 37 642.2 0 37 174 41 642.2 0 41 175 45 642.2 0 45 176 45 642.2 0 45 177 78 642.2 0 78 178 107 642.2 0 107 179 112 642.2 0 112 180 174 642.2 0 174 181 58 642.2 0 58 182 54 642.2 0 54 183 57 642.2 0 57 184 41 642.2 0 41 185 53 642.2 0 53 186 61 642.2 0 61 187 55 642.2 0 55 188 63 642.2 0 63 189 61 642.2 0 61 190 73 642.2 0 73 191 47 642.2 0 47 192 45 642.2 0 45 193 56 642.2 0 56 194 60 642.2 0 60 195 42 642.2 0 42 196 53 642.2 0 53 197 66 642.2 0 66 198 68 642.2 0 68 199 70 642.2 0 70 200 47 642.2 0 47 201 51 642.2 0 51 202 75 642.2 0 75 203 59 642.2 0 59 204 73 642.2 0 73 205 76 642.2 0 76 206 46 642.2 0 46 207 58 642.2 0 58 208 45 642.2 0 45 209 96 642.2 0 96 210 169 642.2 0 169 211 69 642.2 0 69 212 72 642.2 0 72 213 63 642.2 0 63 214 62 642.2 0 62 215 51 642.2 0 51 216 61 642.2 0 61 217 65 642.2 0 65 218 62 642.2 0 62 219 65 642.2 0 65 220 43 642.2 0 43 221 40 642.2 0 40 222 45 642.2 0 45 223 85 642.2 0 85 224 86 642.2 0 86 225 96 642.2 0 96 226 94 642.2 0 94 227 111 642.2 0 111 228 77 642.2 0 77 229 73 642.2 0 73 230 48 642.2 0 48 231 42 642.2 0 42 232 64 642.2 0 64 233 92 642.2 0 92 234 72 642.2 0 72 235 53 642.2 0 53 236 76 642.2 0 76 237 71 642.2 0 71 238 74 642.2 0 74 239 54 642.2 0 54 240 56 642.2 0 56 241 70 642.2 0 70 242 59 642.2 0 59 243 77 642.2 0 77 244 104 642.2 0 104 245 120 642.2 0 120 246 128 642.2 0 128 247 142 642.2 0 142 248 129 642.2 0 129 249 77 642.2 0 77 250 72 642.2 0 72 251 75 642.2 0 75 252 52 642.2 0 52 253 73 642.2 0 73 254 66 642.2 0 66 255 68 642.2 0 68 256 99 642.2 0 99 257 119 642.2 0 119 258 125 642.2 0 125 259 108 642.2 0 108 260 122 642.2 0 122 261 105 642.2 0 105 262 140 642.2 0 140 263 98 642.2 0 98 264 139 642.2 0 139 265 112 642.2 0 112 266 172 642.2 0 172 267 65 642.2 0 65 268 79 642.2 0 79 269 77 642.2 0 77 270 75 642.2 0 75 271 111 642.2 0 111 272 126 642.2 0 126 273 134 642.2 0 134 274 98 642.2 0 98 275 70 642.2 0 70 276 69 642.2 0 69 277 78 642.2 0 78 278 64 642.2 0 64 279 80 642.2 0 80 280 84 642.2 0 84 281 90 642.2 0 90 282 96 642.2 0 96 283 118 642.2 0 118 284 420 642.2 0 420 285 467 642.2 0 467 286 493 642.2 0 493 287 336 642.2 0 336 288 114 642.2 0 114 289 77 642.2 0 77 290 59 642.2 0 59 291 66 642.2 0 66 292 96 642.2 0 96 293 125 642.2 0 125 294 79 642.2 0 79 295 65 642.2 0 65 296 76 642.2 0 76 297 68 642.2 0 68 298 84 642.2 0 84 299 76 642.2 0 76 300 93 642.2 0 93 301 117 642.2 0 117 Finished in 3.19 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 66,823 Reads with adapters: 22,539 (33.7%) Reads that were too short: 143 (0.2%) Reads written (passing filters): 22,396 (33.5%) Total basepairs processed: 18,174,662 bp Total written (filtered): 6,157,723 bp (33.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 22539 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 52 0.0 3 0 52 17 22262 0.0 3 21877 366 17 2 18 33 0.0 3 8 23 2 19 10 0.0 3 0 0 7 3 20 25 0.0 3 0 0 0 25 40 1 0.0 3 1 51 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 70 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 179 1 0.0 3 1 192 2 0.0 3 0 0 0 2 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 217 127 0.0 3 0 0 1 126 218 1 0.0 3 0 0 0 1 242 2 0.0 3 0 1 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 22,396 Reads with adapters: 338 (1.5%) Reads written (passing filters): 22,396 (100.0%) Total basepairs processed: 6,157,723 bp Total written (filtered): 6,156,689 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 338 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 82.5% C: 5.3% G: 11.2% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 322 349.9 0 322 4 12 87.5 0 12 5 4 21.9 0 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.