This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.80 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 449,114 (17.1%) Reads that were too short: 140,673 (5.3%) Reads written (passing filters): 308,441 (11.7%) Total basepairs processed: 791,719,999 bp Total written (filtered): 88,082,888 bp (11.1%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 449114 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1905 642.2 0 1905 7 6326 642.2 0 6326 8 172889 642.2 0 172889 9 105077 642.2 0 105077 10 132 642.2 0 132 11 34 642.2 0 34 12 36 642.2 0 36 13 42 642.2 0 42 14 38 642.2 0 38 15 39 642.2 0 39 16 28 642.2 0 28 17 22 642.2 0 22 18 16 642.2 0 16 19 30 642.2 0 30 20 43 642.2 0 43 21 28 642.2 0 28 22 40 642.2 0 40 23 27 642.2 0 27 24 32 642.2 0 32 25 36 642.2 0 36 26 52 642.2 0 52 27 35 642.2 0 35 28 38 642.2 0 38 29 35 642.2 0 35 30 31 642.2 0 31 31 29 642.2 0 29 32 26 642.2 0 26 33 43 642.2 0 43 34 42 642.2 0 42 35 34 642.2 0 34 36 73 642.2 0 73 37 25 642.2 0 25 38 95 642.2 0 95 39 51 642.2 0 51 40 45 642.2 0 45 41 79 642.2 0 79 42 69 642.2 0 69 43 1157 642.2 0 1157 44 57 642.2 0 57 45 34 642.2 0 34 46 31 642.2 0 31 47 28 642.2 0 28 48 54 642.2 0 54 49 50 642.2 0 50 50 54 642.2 0 54 51 56 642.2 0 56 52 33 642.2 0 33 53 31 642.2 0 31 54 38 642.2 0 38 55 51 642.2 0 51 56 128 642.2 0 128 57 133 642.2 0 133 58 101 642.2 0 101 59 52 642.2 0 52 60 48 642.2 0 48 61 107 642.2 0 107 62 65 642.2 0 65 63 46 642.2 0 46 64 71 642.2 0 71 65 47 642.2 0 47 66 29 642.2 0 29 67 64 642.2 0 64 68 122 642.2 0 122 69 384 642.2 0 384 70 405 642.2 0 405 71 344 642.2 0 344 72 66 642.2 0 66 73 71 642.2 0 71 74 74 642.2 0 74 75 84 642.2 0 84 76 107 642.2 0 107 77 59 642.2 0 59 78 74 642.2 0 74 79 71 642.2 0 71 80 57 642.2 0 57 81 56 642.2 0 56 82 60 642.2 0 60 83 65 642.2 0 65 84 49 642.2 0 49 85 48 642.2 0 48 86 91 642.2 0 91 87 99 642.2 0 99 88 140 642.2 0 140 89 2630 642.2 0 2630 90 842 642.2 0 842 91 2774 642.2 0 2774 92 58 642.2 0 58 93 45 642.2 0 45 94 57 642.2 0 57 95 54 642.2 0 54 96 56 642.2 0 56 97 140 642.2 0 140 98 80 642.2 0 80 99 105 642.2 0 105 100 89 642.2 0 89 101 66 642.2 0 66 102 80 642.2 0 80 103 36 642.2 0 36 104 62 642.2 0 62 105 44 642.2 0 44 106 51 642.2 0 51 107 41 642.2 0 41 108 81 642.2 0 81 109 50 642.2 0 50 110 47 642.2 0 47 111 73 642.2 0 73 112 62 642.2 0 62 113 48 642.2 0 48 114 20 642.2 0 20 115 25 642.2 0 25 116 46 642.2 0 46 117 45 642.2 0 45 118 47 642.2 0 47 119 106 642.2 0 106 120 38 642.2 0 38 121 51 642.2 0 51 122 36 642.2 0 36 123 37 642.2 0 37 124 29 642.2 0 29 125 26 642.2 0 26 126 37 642.2 0 37 127 47 642.2 0 47 128 23 642.2 0 23 129 49 642.2 0 49 130 42 642.2 0 42 131 31 642.2 0 31 132 74 642.2 0 74 133 44 642.2 0 44 134 38 642.2 0 38 135 48 642.2 0 48 136 70 642.2 0 70 137 294 642.2 0 294 138 91 642.2 0 91 139 63 642.2 0 63 140 37 642.2 0 37 141 51 642.2 0 51 142 40 642.2 0 40 143 52 642.2 0 52 144 44 642.2 0 44 145 47 642.2 0 47 146 37 642.2 0 37 147 55 642.2 0 55 148 228 642.2 0 228 149 128 642.2 0 128 150 455 642.2 0 455 151 41 642.2 0 41 152 37 642.2 0 37 153 69 642.2 0 69 154 66 642.2 0 66 155 71 642.2 0 71 156 220 642.2 0 220 157 127 642.2 0 127 158 144 642.2 0 144 159 81 642.2 0 81 160 37 642.2 0 37 161 58 642.2 0 58 162 44 642.2 0 44 163 44 642.2 0 44 164 48 642.2 0 48 165 51 642.2 0 51 166 29 642.2 0 29 167 36 642.2 0 36 168 184 642.2 0 184 169 111 642.2 0 111 170 104 642.2 0 104 171 26 642.2 0 26 172 29 642.2 0 29 173 40 642.2 0 40 174 62 642.2 0 62 175 101 642.2 0 101 176 729 642.2 0 729 177 1352 642.2 0 1352 178 352 642.2 0 352 179 87 642.2 0 87 180 47 642.2 0 47 181 23 642.2 0 23 182 75 642.2 0 75 183 69 642.2 0 69 184 83 642.2 0 83 185 38 642.2 0 38 186 44 642.2 0 44 187 31 642.2 0 31 188 36 642.2 0 36 189 62 642.2 0 62 190 30 642.2 0 30 191 33 642.2 0 33 192 41 642.2 0 41 193 32 642.2 0 32 194 47 642.2 0 47 195 58 642.2 0 58 196 43 642.2 0 43 197 45 642.2 0 45 198 59 642.2 0 59 199 36 642.2 0 36 200 43 642.2 0 43 201 71 642.2 0 71 202 157 642.2 0 157 203 128 642.2 0 128 204 90 642.2 0 90 205 94 642.2 0 94 206 60 642.2 0 60 207 176 642.2 0 176 208 190 642.2 0 190 209 188 642.2 0 188 210 154 642.2 0 154 211 118 642.2 0 118 212 123 642.2 0 123 213 54 642.2 0 54 214 52 642.2 0 52 215 76 642.2 0 76 216 95 642.2 0 95 217 94 642.2 0 94 218 106 642.2 0 106 219 276 642.2 0 276 220 1880 642.2 0 1880 221 1077 642.2 0 1077 222 669 642.2 0 669 223 488 642.2 0 488 224 52 642.2 0 52 225 83 642.2 0 83 226 45 642.2 0 45 227 53 642.2 0 53 228 48 642.2 0 48 229 28 642.2 0 28 230 108 642.2 0 108 231 106 642.2 0 106 232 164 642.2 0 164 233 102 642.2 0 102 234 57 642.2 0 57 235 67 642.2 0 67 236 68 642.2 0 68 237 55 642.2 0 55 238 61 642.2 0 61 239 118 642.2 0 118 240 126 642.2 0 126 241 174 642.2 0 174 242 184 642.2 0 184 243 178 642.2 0 178 244 257 642.2 0 257 245 193 642.2 0 193 246 125 642.2 0 125 247 252 642.2 0 252 248 72 642.2 0 72 249 89 642.2 0 89 250 78 642.2 0 78 251 118 642.2 0 118 252 80 642.2 0 80 253 93 642.2 0 93 254 113 642.2 0 113 255 145 642.2 0 145 256 168 642.2 0 168 257 218 642.2 0 218 258 71 642.2 0 71 259 203 642.2 0 203 260 108 642.2 0 108 261 167 642.2 0 167 262 438 642.2 0 438 263 395 642.2 0 395 264 487 642.2 0 487 265 192 642.2 0 192 266 209 642.2 0 209 267 126 642.2 0 126 268 436 642.2 0 436 269 824 642.2 0 824 270 1076 642.2 0 1076 271 1433 642.2 0 1433 272 213 642.2 0 213 273 97 642.2 0 97 274 583 642.2 0 583 275 322 642.2 0 322 276 815 642.2 0 815 277 266 642.2 0 266 278 563 642.2 0 563 279 721 642.2 0 721 280 1516 642.2 0 1516 281 4041 642.2 0 4041 282 17350 642.2 0 17350 283 37934 642.2 0 37934 284 37046 642.2 0 37046 285 14035 642.2 0 14035 286 6527 642.2 0 6527 287 92 642.2 0 92 288 343 642.2 0 343 289 742 642.2 0 742 290 334 642.2 0 334 291 177 642.2 0 177 292 69 642.2 0 69 293 42 642.2 0 42 294 45 642.2 0 45 295 47 642.2 0 47 296 84 642.2 0 84 297 83 642.2 0 83 298 73 642.2 0 73 299 56 642.2 0 56 300 47 642.2 0 47 301 22 642.2 0 22 Finished in 15.43 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 308,441 Reads with adapters: 110,767 (35.9%) Reads that were too short: 118 (0.0%) Reads written (passing filters): 110,649 (35.9%) Total basepairs processed: 88,082,888 bp Total written (filtered): 29,862,428 bp (33.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 110767 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 4 0.0 3 0 0 0 0 4 17 14 0.0 3 0 0 0 6 8 18 51 0.0 3 0 0 7 21 23 19 546 0.0 3 0 324 57 110 55 20 106738 0.0 4 97910 6814 1451 416 147 21 280 0.0 4 34 201 28 12 5 22 21 0.0 4 1 0 9 7 4 23 688 0.0 4 0 3 1 458 226 24 4 0.0 4 0 0 0 0 4 25 1 0.0 4 0 0 0 0 1 26 2 0.0 4 1 0 0 0 1 27 37 0.0 4 0 0 0 1 36 28 1 0.0 4 0 0 0 0 1 32 2 0.0 4 1 0 0 0 1 35 26 0.0 4 0 0 0 0 26 36 2 0.0 4 2 37 3 0.0 4 1 0 0 0 2 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 1 49 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 1 0.0 4 1 70 2 0.0 4 0 0 0 0 2 71 1 0.0 4 0 0 0 0 1 74 15 0.0 4 0 0 0 0 15 79 2 0.0 4 0 0 0 1 1 83 2 0.0 4 0 0 0 0 2 84 1 0.0 4 1 87 2 0.0 4 0 0 0 0 2 92 3 0.0 4 0 0 0 0 3 93 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 2 0.0 4 0 0 0 0 2 112 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 1 124 2 0.0 4 0 0 0 1 1 125 1 0.0 4 0 0 0 0 1 126 12 0.0 4 0 0 0 0 12 127 2111 0.0 4 0 0 1 0 2110 128 13 0.0 4 0 0 0 1 12 129 2 0.0 4 0 0 0 0 2 130 3 0.0 4 0 0 0 0 3 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 1 140 1 0.0 4 0 1 142 1 0.0 4 1 143 2 0.0 4 1 0 0 0 1 146 14 0.0 4 0 0 0 1 13 147 5 0.0 4 0 1 0 0 4 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 22 0.0 4 0 0 0 0 22 156 1 0.0 4 0 1 157 2 0.0 4 2 160 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 177 2 0.0 4 0 0 0 0 2 187 1 0.0 4 0 0 1 188 1 0.0 4 1 194 1 0.0 4 0 0 0 1 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 1 206 3 0.0 4 0 0 0 0 3 207 1 0.0 4 0 0 0 0 1 208 3 0.0 4 0 0 0 0 3 210 2 0.0 4 0 0 1 1 213 1 0.0 4 0 0 0 0 1 215 4 0.0 4 0 0 0 0 4 218 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 1 225 2 0.0 4 0 0 0 1 1 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 1 228 10 0.0 4 0 0 0 1 9 229 6 0.0 4 0 0 0 1 5 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 232 5 0.0 4 1 0 0 1 3 233 2 0.0 4 0 0 1 0 1 234 5 0.0 4 0 0 0 0 5 235 5 0.0 4 0 0 0 0 5 237 3 0.0 4 0 0 0 1 2 238 1 0.0 4 0 1 242 1 0.0 4 0 0 0 1 243 1 0.0 4 0 0 1 246 1 0.0 4 0 0 0 0 1 248 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 1 251 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 3 0.0 4 0 0 0 0 3 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 1 1 271 1 0.0 4 0 0 0 1 274 2 0.0 4 0 0 0 0 2 282 1 0.0 4 0 0 0 1 283 4 0.0 4 0 0 1 0 3 284 1 0.0 4 0 1 287 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 1 289 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.06 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 110,649 Reads with adapters: 27,666 (25.0%) Reads written (passing filters): 110,649 (100.0%) Total basepairs processed: 29,862,428 bp Total written (filtered): 29,480,278 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 27666 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.7% C: 9.5% G: 84.3% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 80 1728.9 0 80 4 32 432.2 0 32 5 4 108.1 0 4 6 4 27.0 0 4 7 5 6.8 0 5 8 5 1.7 0 5 9 4873 0.4 0 638 4235 10 19719 0.1 1 1531 18188 11 2181 0.0 1 104 2077 12 55 0.0 1 1 54 13 62 0.0 1 0 62 14 1 0.0 1 0 1 16 1 0.0 1 0 1 18 12 0.0 1 9 3 19 59 0.0 1 36 23 20 3 0.0 1 1 2 21 1 0.0 1 1 22 16 0.0 1 9 7 23 1 0.0 1 0 1 25 1 0.0 1 0 1 35 2 0.0 1 1 1 43 1 0.0 1 1 44 1 0.0 1 1 50 1 0.0 1 0 1 56 3 0.0 1 2 1 58 4 0.0 1 4 62 1 0.0 1 0 1 74 1 0.0 1 0 1 80 23 0.0 1 13 10 81 1 0.0 1 1 89 1 0.0 1 0 1 95 1 0.0 1 1 102 1 0.0 1 1 106 4 0.0 1 3 1 107 1 0.0 1 1 108 1 0.0 1 1 112 1 0.0 1 1 113 1 0.0 1 1 114 23 0.0 1 18 5 122 2 0.0 1 1 1 126 10 0.0 1 9 1 127 1 0.0 1 1 128 1 0.0 1 1 144 1 0.0 1 1 145 1 0.0 1 1 147 1 0.0 1 1 148 2 0.0 1 2 150 7 0.0 1 5 2 155 1 0.0 1 1 156 21 0.0 1 18 3 161 1 0.0 1 1 168 1 0.0 1 1 176 1 0.0 1 1 190 1 0.0 1 1 196 4 0.0 1 4 197 1 0.0 1 1 201 1 0.0 1 0 1 208 204 0.0 1 171 33 209 5 0.0 1 4 1 210 5 0.0 1 5 211 1 0.0 1 1 220 1 0.0 1 1 223 5 0.0 1 4 1 224 1 0.0 1 1 227 1 0.0 1 1 228 5 0.0 1 5 231 1 0.0 1 1 247 1 0.0 1 1 256 1 0.0 1 1 257 2 0.0 1 2 261 170 0.0 1 159 11 262 12 0.0 1 11 1 264 2 0.0 1 2 265 2 0.0 1 2 271 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.