This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.67 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 475,083 (18.1%) Reads that were too short: 149,214 (5.7%) Reads written (passing filters): 325,869 (12.4%) Total basepairs processed: 791,719,999 bp Total written (filtered): 93,636,018 bp (11.8%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 475083 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1839 642.2 0 1839 7 4876 642.2 0 4876 8 164610 642.2 0 164610 9 137580 642.2 0 137580 10 144 642.2 0 144 11 23 642.2 0 23 12 23 642.2 0 23 13 12 642.2 0 12 14 18 642.2 0 18 15 17 642.2 0 17 16 16 642.2 0 16 17 11 642.2 0 11 18 12 642.2 0 12 19 15 642.2 0 15 20 13 642.2 0 13 21 87 642.2 0 87 22 15 642.2 0 15 23 16 642.2 0 16 24 14 642.2 0 14 25 19 642.2 0 19 26 19 642.2 0 19 27 12 642.2 0 12 28 27 642.2 0 27 29 15 642.2 0 15 30 9 642.2 0 9 31 22 642.2 0 22 32 22 642.2 0 22 33 16 642.2 0 16 34 10 642.2 0 10 35 16 642.2 0 16 36 27 642.2 0 27 37 16 642.2 0 16 38 52 642.2 0 52 39 34 642.2 0 34 40 23 642.2 0 23 41 50 642.2 0 50 42 50 642.2 0 50 43 1295 642.2 0 1295 44 54 642.2 0 54 45 20 642.2 0 20 46 23 642.2 0 23 47 11 642.2 0 11 48 17 642.2 0 17 49 43 642.2 0 43 50 29 642.2 0 29 51 29 642.2 0 29 52 30 642.2 0 30 53 20 642.2 0 20 54 22 642.2 0 22 55 20 642.2 0 20 56 418 642.2 0 418 57 93 642.2 0 93 58 22 642.2 0 22 59 38 642.2 0 38 60 22 642.2 0 22 61 50 642.2 0 50 62 24 642.2 0 24 63 19 642.2 0 19 64 43 642.2 0 43 65 30 642.2 0 30 66 14 642.2 0 14 67 33 642.2 0 33 68 45 642.2 0 45 69 130 642.2 0 130 70 123 642.2 0 123 71 104 642.2 0 104 72 40 642.2 0 40 73 36 642.2 0 36 74 35 642.2 0 35 75 45 642.2 0 45 76 60 642.2 0 60 77 28 642.2 0 28 78 27 642.2 0 27 79 34 642.2 0 34 80 40 642.2 0 40 81 27 642.2 0 27 82 43 642.2 0 43 83 48 642.2 0 48 84 48 642.2 0 48 85 45 642.2 0 45 86 49 642.2 0 49 87 62 642.2 0 62 88 100 642.2 0 100 89 2581 642.2 0 2581 90 597 642.2 0 597 91 2252 642.2 0 2252 92 47 642.2 0 47 93 30 642.2 0 30 94 27 642.2 0 27 95 17 642.2 0 17 96 14 642.2 0 14 97 44 642.2 0 44 98 40 642.2 0 40 99 33 642.2 0 33 100 39 642.2 0 39 101 26 642.2 0 26 102 26 642.2 0 26 103 25 642.2 0 25 104 20 642.2 0 20 105 30 642.2 0 30 106 20 642.2 0 20 107 18 642.2 0 18 108 32 642.2 0 32 109 29 642.2 0 29 110 31 642.2 0 31 111 44 642.2 0 44 112 35 642.2 0 35 113 15 642.2 0 15 114 15 642.2 0 15 115 16 642.2 0 16 116 55 642.2 0 55 117 26 642.2 0 26 118 37 642.2 0 37 119 93 642.2 0 93 120 23 642.2 0 23 121 21 642.2 0 21 122 16 642.2 0 16 123 18 642.2 0 18 124 13 642.2 0 13 125 22 642.2 0 22 126 16 642.2 0 16 127 20 642.2 0 20 128 20 642.2 0 20 129 22 642.2 0 22 130 18 642.2 0 18 131 24 642.2 0 24 132 28 642.2 0 28 133 18 642.2 0 18 134 17 642.2 0 17 135 27 642.2 0 27 136 39 642.2 0 39 137 295 642.2 0 295 138 74 642.2 0 74 139 44 642.2 0 44 140 29 642.2 0 29 141 20 642.2 0 20 142 18 642.2 0 18 143 31 642.2 0 31 144 28 642.2 0 28 145 28 642.2 0 28 146 16 642.2 0 16 147 16 642.2 0 16 148 65 642.2 0 65 149 42 642.2 0 42 150 110 642.2 0 110 151 25 642.2 0 25 152 22 642.2 0 22 153 20 642.2 0 20 154 26 642.2 0 26 155 46 642.2 0 46 156 233 642.2 0 233 157 43 642.2 0 43 158 93 642.2 0 93 159 40 642.2 0 40 160 18 642.2 0 18 161 21 642.2 0 21 162 41 642.2 0 41 163 19 642.2 0 19 164 19 642.2 0 19 165 31 642.2 0 31 166 22 642.2 0 22 167 26 642.2 0 26 168 206 642.2 0 206 169 98 642.2 0 98 170 73 642.2 0 73 171 20 642.2 0 20 172 20 642.2 0 20 173 39 642.2 0 39 174 51 642.2 0 51 175 89 642.2 0 89 176 913 642.2 0 913 177 1857 642.2 0 1857 178 375 642.2 0 375 179 28 642.2 0 28 180 21 642.2 0 21 181 12 642.2 0 12 182 31 642.2 0 31 183 29 642.2 0 29 184 26 642.2 0 26 185 16 642.2 0 16 186 27 642.2 0 27 187 23 642.2 0 23 188 16 642.2 0 16 189 36 642.2 0 36 190 20 642.2 0 20 191 13 642.2 0 13 192 23 642.2 0 23 193 22 642.2 0 22 194 24 642.2 0 24 195 20 642.2 0 20 196 25 642.2 0 25 197 17 642.2 0 17 198 25 642.2 0 25 199 20 642.2 0 20 200 24 642.2 0 24 201 25 642.2 0 25 202 78 642.2 0 78 203 61 642.2 0 61 204 38 642.2 0 38 205 35 642.2 0 35 206 34 642.2 0 34 207 113 642.2 0 113 208 120 642.2 0 120 209 87 642.2 0 87 210 65 642.2 0 65 211 59 642.2 0 59 212 73 642.2 0 73 213 31 642.2 0 31 214 35 642.2 0 35 215 37 642.2 0 37 216 55 642.2 0 55 217 46 642.2 0 46 218 68 642.2 0 68 219 175 642.2 0 175 220 2020 642.2 0 2020 221 1091 642.2 0 1091 222 556 642.2 0 556 223 435 642.2 0 435 224 29 642.2 0 29 225 42 642.2 0 42 226 26 642.2 0 26 227 38 642.2 0 38 228 27 642.2 0 27 229 22 642.2 0 22 230 48 642.2 0 48 231 55 642.2 0 55 232 76 642.2 0 76 233 61 642.2 0 61 234 42 642.2 0 42 235 54 642.2 0 54 236 25 642.2 0 25 237 34 642.2 0 34 238 62 642.2 0 62 239 57 642.2 0 57 240 63 642.2 0 63 241 83 642.2 0 83 242 80 642.2 0 80 243 80 642.2 0 80 244 156 642.2 0 156 245 180 642.2 0 180 246 98 642.2 0 98 247 291 642.2 0 291 248 53 642.2 0 53 249 66 642.2 0 66 250 56 642.2 0 56 251 78 642.2 0 78 252 59 642.2 0 59 253 74 642.2 0 74 254 89 642.2 0 89 255 99 642.2 0 99 256 130 642.2 0 130 257 187 642.2 0 187 258 57 642.2 0 57 259 286 642.2 0 286 260 58 642.2 0 58 261 93 642.2 0 93 262 124 642.2 0 124 263 153 642.2 0 153 264 190 642.2 0 190 265 104 642.2 0 104 266 188 642.2 0 188 267 95 642.2 0 95 268 200 642.2 0 200 269 353 642.2 0 353 270 548 642.2 0 548 271 1154 642.2 0 1154 272 170 642.2 0 170 273 94 642.2 0 94 274 819 642.2 0 819 275 520 642.2 0 520 276 1075 642.2 0 1075 277 226 642.2 0 226 278 806 642.2 0 806 279 822 642.2 0 822 280 2047 642.2 0 2047 281 6358 642.2 0 6358 282 27714 642.2 0 27714 283 32123 642.2 0 32123 284 44079 642.2 0 44079 285 15151 642.2 0 15151 286 4404 642.2 0 4404 287 131 642.2 0 131 288 131 642.2 0 131 289 317 642.2 0 317 290 130 642.2 0 130 291 107 642.2 0 107 292 87 642.2 0 87 293 43 642.2 0 43 294 28 642.2 0 28 295 32 642.2 0 32 296 43 642.2 0 43 297 68 642.2 0 68 298 59 642.2 0 59 299 55 642.2 0 55 300 31 642.2 0 31 301 9 642.2 0 9 Finished in 14.08 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 325,869 Reads with adapters: 167,490 (51.4%) Reads that were too short: 188 (0.1%) Reads written (passing filters): 167,302 (51.3%) Total basepairs processed: 93,636,018 bp Total written (filtered): 46,156,824 bp (49.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 167490 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 12 0.0 3 0 0 10 2 16 432 0.0 3 0 420 12 17 166119 0.0 3 163513 2521 75 10 18 372 0.0 3 24 333 11 4 19 31 0.0 3 0 0 24 7 20 181 0.0 3 0 1 0 180 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 0 1 28 1 0.0 3 1 29 1 0.0 3 0 1 31 1 0.0 3 1 39 1 0.0 3 1 40 1 0.0 3 0 0 0 1 43 4 0.0 3 0 0 0 4 65 5 0.0 3 0 0 0 5 66 1 0.0 3 0 0 0 1 80 2 0.0 3 1 0 0 1 82 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 106 0.0 3 0 0 1 105 102 11 0.0 3 0 0 0 11 104 1 0.0 3 1 105 4 0.0 3 0 0 0 4 127 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 167 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 7 0.0 3 0 0 0 7 194 1 0.0 3 0 0 0 1 201 1 0.0 3 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 150 0.0 3 0 0 5 145 228 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 2 0.0 3 0 0 0 2 280 5 0.0 3 0 0 0 5 281 7 0.0 3 0 0 0 7 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.98 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 167,302 Reads with adapters: 4,956 (3.0%) Reads written (passing filters): 167,302 (100.0%) Total basepairs processed: 46,156,824 bp Total written (filtered): 46,141,284 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 4956 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 70.1% C: 10.0% G: 17.2% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4539 2614.1 0 4539 4 255 653.5 0 255 5 72 163.4 0 72 6 87 40.8 0 87 7 3 10.2 0 3