This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.09 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 263,470 (10.0%) Reads that were too short: 25,844 (1.0%) Reads written (passing filters): 237,626 (9.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 59,768,996 bp (7.5%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 263470 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1879 642.2 0 1879 7 83268 642.2 0 83268 8 68976 642.2 0 68976 9 142 642.2 0 142 10 92 642.2 0 92 11 93 642.2 0 93 12 93 642.2 0 93 13 91 642.2 0 91 14 107 642.2 0 107 15 75 642.2 0 75 16 79 642.2 0 79 17 110 642.2 0 110 18 164 642.2 0 164 19 84 642.2 0 84 20 169 642.2 0 169 21 88 642.2 0 88 22 84 642.2 0 84 23 103 642.2 0 103 24 90 642.2 0 90 25 76 642.2 0 76 26 75 642.2 0 75 27 97 642.2 0 97 28 70 642.2 0 70 29 111 642.2 0 111 30 58 642.2 0 58 31 81 642.2 0 81 32 90 642.2 0 90 33 146 642.2 0 146 34 111 642.2 0 111 35 186 642.2 0 186 36 72 642.2 0 72 37 100 642.2 0 100 38 62 642.2 0 62 39 90 642.2 0 90 40 88 642.2 0 88 41 74 642.2 0 74 42 92 642.2 0 92 43 85 642.2 0 85 44 64 642.2 0 64 45 115 642.2 0 115 46 86 642.2 0 86 47 104 642.2 0 104 48 77 642.2 0 77 49 149 642.2 0 149 50 427 642.2 0 427 51 79 642.2 0 79 52 99 642.2 0 99 53 89 642.2 0 89 54 93 642.2 0 93 55 108 642.2 0 108 56 126 642.2 0 126 57 88 642.2 0 88 58 134 642.2 0 134 59 108 642.2 0 108 60 105 642.2 0 105 61 83 642.2 0 83 62 106 642.2 0 106 63 120 642.2 0 120 64 129 642.2 0 129 65 175 642.2 0 175 66 227 642.2 0 227 67 410 642.2 0 410 68 443 642.2 0 443 69 208 642.2 0 208 70 209 642.2 0 209 71 129 642.2 0 129 72 151 642.2 0 151 73 180 642.2 0 180 74 142 642.2 0 142 75 182 642.2 0 182 76 197 642.2 0 197 77 280 642.2 0 280 78 428 642.2 0 428 79 700 642.2 0 700 80 9299 642.2 0 9299 81 6486 642.2 0 6486 82 12252 642.2 0 12252 83 196 642.2 0 196 84 136 642.2 0 136 85 116 642.2 0 116 86 150 642.2 0 150 87 142 642.2 0 142 88 306 642.2 0 306 89 101 642.2 0 101 90 58 642.2 0 58 91 61 642.2 0 61 92 57 642.2 0 57 93 83 642.2 0 83 94 93 642.2 0 93 95 141 642.2 0 141 96 172 642.2 0 172 97 106 642.2 0 106 98 52 642.2 0 52 99 100 642.2 0 100 100 62 642.2 0 62 101 46 642.2 0 46 102 79 642.2 0 79 103 92 642.2 0 92 104 70 642.2 0 70 105 76 642.2 0 76 106 57 642.2 0 57 107 70 642.2 0 70 108 72 642.2 0 72 109 62 642.2 0 62 110 83 642.2 0 83 111 188 642.2 0 188 112 67 642.2 0 67 113 113 642.2 0 113 114 95 642.2 0 95 115 66 642.2 0 66 116 76 642.2 0 76 117 162 642.2 0 162 118 117 642.2 0 117 119 271 642.2 0 271 120 361 642.2 0 361 121 258 642.2 0 258 122 364 642.2 0 364 123 135 642.2 0 135 124 172 642.2 0 172 125 158 642.2 0 158 126 258 642.2 0 258 127 1284 642.2 0 1284 128 154 642.2 0 154 129 451 642.2 0 451 130 120 642.2 0 120 131 77 642.2 0 77 132 68 642.2 0 68 133 87 642.2 0 87 134 86 642.2 0 86 135 196 642.2 0 196 136 75 642.2 0 75 137 108 642.2 0 108 138 84 642.2 0 84 139 108 642.2 0 108 140 99 642.2 0 99 141 84 642.2 0 84 142 315 642.2 0 315 143 95 642.2 0 95 144 102 642.2 0 102 145 128 642.2 0 128 146 180 642.2 0 180 147 147 642.2 0 147 148 445 642.2 0 445 149 93 642.2 0 93 150 117 642.2 0 117 151 61 642.2 0 61 152 111 642.2 0 111 153 111 642.2 0 111 154 114 642.2 0 114 155 138 642.2 0 138 156 74 642.2 0 74 157 60 642.2 0 60 158 82 642.2 0 82 159 80 642.2 0 80 160 64 642.2 0 64 161 71 642.2 0 71 162 110 642.2 0 110 163 72 642.2 0 72 164 127 642.2 0 127 165 99 642.2 0 99 166 102 642.2 0 102 167 119 642.2 0 119 168 229 642.2 0 229 169 115 642.2 0 115 170 100 642.2 0 100 171 105 642.2 0 105 172 102 642.2 0 102 173 84 642.2 0 84 174 121 642.2 0 121 175 179 642.2 0 179 176 130 642.2 0 130 177 175 642.2 0 175 178 184 642.2 0 184 179 123 642.2 0 123 180 184 642.2 0 184 181 243 642.2 0 243 182 203 642.2 0 203 183 277 642.2 0 277 184 340 642.2 0 340 185 382 642.2 0 382 186 590 642.2 0 590 187 609 642.2 0 609 188 8896 642.2 0 8896 189 4761 642.2 0 4761 190 11383 642.2 0 11383 191 661 642.2 0 661 192 359 642.2 0 359 193 781 642.2 0 781 194 410 642.2 0 410 195 215 642.2 0 215 196 175 642.2 0 175 197 157 642.2 0 157 198 942 642.2 0 942 199 91 642.2 0 91 200 89 642.2 0 89 201 95 642.2 0 95 202 109 642.2 0 109 203 104 642.2 0 104 204 122 642.2 0 122 205 195 642.2 0 195 206 1417 642.2 0 1417 207 1094 642.2 0 1094 208 1657 642.2 0 1657 209 161 642.2 0 161 210 173 642.2 0 173 211 416 642.2 0 416 212 1130 642.2 0 1130 213 584 642.2 0 584 214 431 642.2 0 431 215 269 642.2 0 269 216 69 642.2 0 69 217 57 642.2 0 57 218 112 642.2 0 112 219 91 642.2 0 91 220 76 642.2 0 76 221 70 642.2 0 70 222 106 642.2 0 106 223 86 642.2 0 86 224 69 642.2 0 69 225 200 642.2 0 200 226 137 642.2 0 137 227 175 642.2 0 175 228 125 642.2 0 125 229 156 642.2 0 156 230 189 642.2 0 189 231 167 642.2 0 167 232 106 642.2 0 106 233 122 642.2 0 122 234 97 642.2 0 97 235 135 642.2 0 135 236 87 642.2 0 87 237 113 642.2 0 113 238 99 642.2 0 99 239 76 642.2 0 76 240 102 642.2 0 102 241 175 642.2 0 175 242 112 642.2 0 112 243 74 642.2 0 74 244 113 642.2 0 113 245 88 642.2 0 88 246 86 642.2 0 86 247 110 642.2 0 110 248 151 642.2 0 151 249 111 642.2 0 111 250 123 642.2 0 123 251 136 642.2 0 136 252 140 642.2 0 140 253 138 642.2 0 138 254 136 642.2 0 136 255 183 642.2 0 183 256 242 642.2 0 242 257 221 642.2 0 221 258 216 642.2 0 216 259 221 642.2 0 221 260 177 642.2 0 177 261 100 642.2 0 100 262 147 642.2 0 147 263 107 642.2 0 107 264 101 642.2 0 101 265 83 642.2 0 83 266 137 642.2 0 137 267 163 642.2 0 163 268 499 642.2 0 499 269 576 642.2 0 576 270 618 642.2 0 618 271 1195 642.2 0 1195 272 1435 642.2 0 1435 273 1338 642.2 0 1338 274 339 642.2 0 339 275 91 642.2 0 91 276 87 642.2 0 87 277 85 642.2 0 85 278 132 642.2 0 132 279 148 642.2 0 148 280 137 642.2 0 137 281 149 642.2 0 149 282 373 642.2 0 373 283 469 642.2 0 469 284 538 642.2 0 538 285 83 642.2 0 83 286 90 642.2 0 90 287 123 642.2 0 123 288 147 642.2 0 147 289 134 642.2 0 134 290 111 642.2 0 111 291 145 642.2 0 145 292 382 642.2 0 382 293 347 642.2 0 347 294 310 642.2 0 310 295 225 642.2 0 225 296 135 642.2 0 135 297 177 642.2 0 177 298 170 642.2 0 170 299 134 642.2 0 134 300 216 642.2 0 216 301 231 642.2 0 231 Finished in 11.13 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 237,626 Reads with adapters: 72,523 (30.5%) Reads that were too short: 147 (0.1%) Reads written (passing filters): 72,376 (30.5%) Total basepairs processed: 59,768,996 bp Total written (filtered): 19,732,023 bp (33.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 72523 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 26 0.0 3 0 0 0 4 22 18 33 0.0 3 0 0 23 6 4 19 1071 0.0 3 0 805 140 95 31 20 70489 0.0 4 65235 4187 807 190 70 21 195 0.0 4 41 130 11 9 4 22 38 0.0 4 4 7 15 6 6 23 241 0.0 4 0 0 0 164 77 24 8 0.0 4 1 0 0 1 6 25 3 0.0 4 1 0 2 28 1 0.0 4 0 0 0 0 1 29 2 0.0 4 0 0 0 0 2 30 3 0.0 4 0 0 0 0 3 39 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 53 2 0.0 4 0 0 0 0 2 55 13 0.0 4 0 0 0 0 13 62 1 0.0 4 0 0 0 0 1 72 2 0.0 4 0 0 0 0 2 78 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 1 93 1 0.0 4 0 0 0 0 1 94 4 0.0 4 0 0 1 1 2 95 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 238 0.0 4 0 0 0 0 238 128 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 135 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 147 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 154 6 0.0 4 0 0 0 0 6 155 48 0.0 4 0 0 0 2 46 156 25 0.0 4 0 0 0 3 22 157 4 0.0 4 0 0 0 1 3 159 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 1 170 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 5 0.0 4 0 0 0 0 5 227 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 236 1 0.0 4 1 244 1 0.0 4 0 0 0 0 1 245 1 0.0 4 1 247 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 284 7 0.0 4 0 0 0 0 7 285 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 291 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.59 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 72,376 Reads with adapters: 11,785 (16.3%) Reads written (passing filters): 72,376 (100.0%) Total basepairs processed: 19,732,023 bp Total written (filtered): 19,600,057 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11785 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 8.3% G: 86.0% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 113 1130.9 0 113 4 308 282.7 0 308 5 6 70.7 0 6 6 8 17.7 0 8 7 3 4.4 0 3 8 6 1.1 0 6 9 82 0.3 0 11 71 10 374 0.1 1 39 335 11 10612 0.0 1 381 10231 12 129 0.0 1 8 121 13 108 0.0 1 2 106 14 9 0.0 1 0 9 109 1 0.0 1 1 156 1 0.0 1 1 157 1 0.0 1 0 1 192 1 0.0 1 1 209 2 0.0 1 2 214 1 0.0 1 1 224 11 0.0 1 10 1 225 1 0.0 1 1 229 1 0.0 1 1 253 2 0.0 1 2 256 1 0.0 1 1 258 2 0.0 1 2 259 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.