This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.57 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 255,506 (9.7%) Reads that were too short: 16,385 (0.6%) Reads written (passing filters): 239,121 (9.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 60,867,292 bp (7.7%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 255506 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 996 642.2 0 996 7 77946 642.2 0 77946 8 82862 642.2 0 82862 9 111 642.2 0 111 10 61 642.2 0 61 11 54 642.2 0 54 12 44 642.2 0 44 13 33 642.2 0 33 14 54 642.2 0 54 15 48 642.2 0 48 16 45 642.2 0 45 17 34 642.2 0 34 18 64 642.2 0 64 19 65 642.2 0 65 20 84 642.2 0 84 21 65 642.2 0 65 22 68 642.2 0 68 23 62 642.2 0 62 24 46 642.2 0 46 25 51 642.2 0 51 26 63 642.2 0 63 27 68 642.2 0 68 28 55 642.2 0 55 29 111 642.2 0 111 30 69 642.2 0 69 31 80 642.2 0 80 32 65 642.2 0 65 33 131 642.2 0 131 34 79 642.2 0 79 35 168 642.2 0 168 36 54 642.2 0 54 37 81 642.2 0 81 38 50 642.2 0 50 39 76 642.2 0 76 40 75 642.2 0 75 41 73 642.2 0 73 42 77 642.2 0 77 43 85 642.2 0 85 44 46 642.2 0 46 45 97 642.2 0 97 46 51 642.2 0 51 47 78 642.2 0 78 48 154 642.2 0 154 49 101 642.2 0 101 50 423 642.2 0 423 51 57 642.2 0 57 52 94 642.2 0 94 53 86 642.2 0 86 54 68 642.2 0 68 55 73 642.2 0 73 56 82 642.2 0 82 57 60 642.2 0 60 58 68 642.2 0 68 59 91 642.2 0 91 60 83 642.2 0 83 61 66 642.2 0 66 62 101 642.2 0 101 63 102 642.2 0 102 64 122 642.2 0 122 65 131 642.2 0 131 66 232 642.2 0 232 67 422 642.2 0 422 68 663 642.2 0 663 69 194 642.2 0 194 70 189 642.2 0 189 71 112 642.2 0 112 72 108 642.2 0 108 73 119 642.2 0 119 74 143 642.2 0 143 75 137 642.2 0 137 76 160 642.2 0 160 77 224 642.2 0 224 78 423 642.2 0 423 79 516 642.2 0 516 80 9205 642.2 0 9205 81 6495 642.2 0 6495 82 12655 642.2 0 12655 83 94 642.2 0 94 84 61 642.2 0 61 85 52 642.2 0 52 86 61 642.2 0 61 87 118 642.2 0 118 88 226 642.2 0 226 89 65 642.2 0 65 90 44 642.2 0 44 91 53 642.2 0 53 92 43 642.2 0 43 93 45 642.2 0 45 94 50 642.2 0 50 95 71 642.2 0 71 96 73 642.2 0 73 97 61 642.2 0 61 98 55 642.2 0 55 99 62 642.2 0 62 100 51 642.2 0 51 101 32 642.2 0 32 102 51 642.2 0 51 103 67 642.2 0 67 104 56 642.2 0 56 105 41 642.2 0 41 106 61 642.2 0 61 107 55 642.2 0 55 108 61 642.2 0 61 109 71 642.2 0 71 110 75 642.2 0 75 111 210 642.2 0 210 112 57 642.2 0 57 113 84 642.2 0 84 114 56 642.2 0 56 115 56 642.2 0 56 116 55 642.2 0 55 117 84 642.2 0 84 118 52 642.2 0 52 119 129 642.2 0 129 120 134 642.2 0 134 121 112 642.2 0 112 122 189 642.2 0 189 123 69 642.2 0 69 124 76 642.2 0 76 125 83 642.2 0 83 126 121 642.2 0 121 127 1074 642.2 0 1074 128 105 642.2 0 105 129 378 642.2 0 378 130 130 642.2 0 130 131 63 642.2 0 63 132 50 642.2 0 50 133 70 642.2 0 70 134 73 642.2 0 73 135 184 642.2 0 184 136 74 642.2 0 74 137 63 642.2 0 63 138 66 642.2 0 66 139 59 642.2 0 59 140 62 642.2 0 62 141 74 642.2 0 74 142 331 642.2 0 331 143 95 642.2 0 95 144 69 642.2 0 69 145 108 642.2 0 108 146 158 642.2 0 158 147 86 642.2 0 86 148 453 642.2 0 453 149 52 642.2 0 52 150 101 642.2 0 101 151 59 642.2 0 59 152 70 642.2 0 70 153 69 642.2 0 69 154 47 642.2 0 47 155 97 642.2 0 97 156 26 642.2 0 26 157 48 642.2 0 48 158 57 642.2 0 57 159 60 642.2 0 60 160 57 642.2 0 57 161 61 642.2 0 61 162 69 642.2 0 69 163 72 642.2 0 72 164 112 642.2 0 112 165 96 642.2 0 96 166 76 642.2 0 76 167 83 642.2 0 83 168 204 642.2 0 204 169 100 642.2 0 100 170 74 642.2 0 74 171 108 642.2 0 108 172 86 642.2 0 86 173 58 642.2 0 58 174 79 642.2 0 79 175 162 642.2 0 162 176 98 642.2 0 98 177 132 642.2 0 132 178 164 642.2 0 164 179 83 642.2 0 83 180 124 642.2 0 124 181 213 642.2 0 213 182 206 642.2 0 206 183 243 642.2 0 243 184 300 642.2 0 300 185 348 642.2 0 348 186 471 642.2 0 471 187 465 642.2 0 465 188 8318 642.2 0 8318 189 4906 642.2 0 4906 190 12441 642.2 0 12441 191 428 642.2 0 428 192 177 642.2 0 177 193 367 642.2 0 367 194 179 642.2 0 179 195 110 642.2 0 110 196 121 642.2 0 121 197 133 642.2 0 133 198 995 642.2 0 995 199 60 642.2 0 60 200 58 642.2 0 58 201 67 642.2 0 67 202 66 642.2 0 66 203 61 642.2 0 61 204 77 642.2 0 77 205 91 642.2 0 91 206 963 642.2 0 963 207 812 642.2 0 812 208 1507 642.2 0 1507 209 42 642.2 0 42 210 89 642.2 0 89 211 128 642.2 0 128 212 287 642.2 0 287 213 212 642.2 0 212 214 126 642.2 0 126 215 320 642.2 0 320 216 61 642.2 0 61 217 66 642.2 0 66 218 113 642.2 0 113 219 70 642.2 0 70 220 56 642.2 0 56 221 56 642.2 0 56 222 100 642.2 0 100 223 61 642.2 0 61 224 44 642.2 0 44 225 177 642.2 0 177 226 82 642.2 0 82 227 154 642.2 0 154 228 70 642.2 0 70 229 54 642.2 0 54 230 76 642.2 0 76 231 80 642.2 0 80 232 42 642.2 0 42 233 60 642.2 0 60 234 57 642.2 0 57 235 51 642.2 0 51 236 54 642.2 0 54 237 81 642.2 0 81 238 47 642.2 0 47 239 43 642.2 0 43 240 49 642.2 0 49 241 89 642.2 0 89 242 66 642.2 0 66 243 45 642.2 0 45 244 71 642.2 0 71 245 47 642.2 0 47 246 45 642.2 0 45 247 63 642.2 0 63 248 73 642.2 0 73 249 52 642.2 0 52 250 64 642.2 0 64 251 92 642.2 0 92 252 86 642.2 0 86 253 110 642.2 0 110 254 75 642.2 0 75 255 80 642.2 0 80 256 114 642.2 0 114 257 100 642.2 0 100 258 101 642.2 0 101 259 138 642.2 0 138 260 72 642.2 0 72 261 62 642.2 0 62 262 87 642.2 0 87 263 76 642.2 0 76 264 60 642.2 0 60 265 61 642.2 0 61 266 78 642.2 0 78 267 82 642.2 0 82 268 131 642.2 0 131 269 175 642.2 0 175 270 190 642.2 0 190 271 1119 642.2 0 1119 272 1463 642.2 0 1463 273 1006 642.2 0 1006 274 175 642.2 0 175 275 49 642.2 0 49 276 39 642.2 0 39 277 67 642.2 0 67 278 76 642.2 0 76 279 82 642.2 0 82 280 82 642.2 0 82 281 113 642.2 0 113 282 196 642.2 0 196 283 249 642.2 0 249 284 441 642.2 0 441 285 67 642.2 0 67 286 61 642.2 0 61 287 85 642.2 0 85 288 87 642.2 0 87 289 78 642.2 0 78 290 96 642.2 0 96 291 107 642.2 0 107 292 292 642.2 0 292 293 280 642.2 0 280 294 198 642.2 0 198 295 164 642.2 0 164 296 93 642.2 0 93 297 69 642.2 0 69 298 79 642.2 0 79 299 59 642.2 0 59 300 115 642.2 0 115 301 128 642.2 0 128 Finished in 10.13 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 239,121 Reads with adapters: 76,468 (32.0%) Reads that were too short: 46 (0.0%) Reads written (passing filters): 76,422 (32.0%) Total basepairs processed: 60,867,292 bp Total written (filtered): 21,145,982 bp (34.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 76468 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 357 0.0 3 0 352 5 17 75692 0.0 3 74201 1457 33 1 18 157 0.0 3 17 137 3 19 39 0.0 3 9 0 25 5 20 7 0.0 3 1 0 0 6 22 2 0.0 3 1 0 0 1 23 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 36 1 0.0 3 1 43 7 0.0 3 0 0 0 7 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 49 5 0.0 3 0 0 0 5 50 2 0.0 3 0 0 0 2 52 3 0.0 3 2 1 53 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 3 0.0 3 0 0 0 3 64 4 0.0 3 0 0 0 4 65 5 0.0 3 0 0 0 5 68 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 72 2 0.0 3 0 0 1 1 73 1 0.0 3 0 0 0 1 77 5 0.0 3 0 0 0 5 81 4 0.0 3 0 0 0 4 86 22 0.0 3 0 0 0 22 87 3 0.0 3 0 0 0 3 89 2 0.0 3 0 0 0 2 91 4 0.0 3 0 0 0 4 92 44 0.0 3 0 0 0 44 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 20 0.0 3 0 0 0 20 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 1 115 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 133 1 0.0 3 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 151 2 0.0 3 0 0 0 2 155 2 0.0 3 0 0 0 2 167 1 0.0 3 1 169 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 189 2 0.0 3 0 0 0 2 191 8 0.0 3 0 0 1 7 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 254 2 0.0 3 0 0 0 2 259 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 286 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.64 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 76,422 Reads with adapters: 80 (0.1%) Reads written (passing filters): 76,422 (100.0%) Total basepairs processed: 21,145,982 bp Total written (filtered): 21,145,721 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 80 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 30.0% C: 12.5% G: 22.5% T: 35.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 63 1194.1 0 63 4 14 298.5 0 14 5 2 74.6 0 2 6 1 18.7 0 1