This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.67 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 90,716 (3.4%) Reads that were too short: 41,287 (1.6%) Reads written (passing filters): 49,429 (1.9%) Total basepairs processed: 791,719,999 bp Total written (filtered): 9,226,339 bp (1.2%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 90716 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 158 642.2 0 158 7 2197 642.2 0 2197 8 173 642.2 0 173 9 156 642.2 0 156 10 171 642.2 0 171 11 504 642.2 0 504 12 105 642.2 0 105 13 175 642.2 0 175 14 176 642.2 0 176 15 144 642.2 0 144 16 127 642.2 0 127 17 110 642.2 0 110 18 120 642.2 0 120 19 129 642.2 0 129 20 133 642.2 0 133 21 117 642.2 0 117 22 124 642.2 0 124 23 176 642.2 0 176 24 146 642.2 0 146 25 156 642.2 0 156 26 149 642.2 0 149 27 159 642.2 0 159 28 115 642.2 0 115 29 133 642.2 0 133 30 123 642.2 0 123 31 146 642.2 0 146 32 149 642.2 0 149 33 118 642.2 0 118 34 141 642.2 0 141 35 119 642.2 0 119 36 133 642.2 0 133 37 110 642.2 0 110 38 122 642.2 0 122 39 136 642.2 0 136 40 136 642.2 0 136 41 100 642.2 0 100 42 133 642.2 0 133 43 197 642.2 0 197 44 155 642.2 0 155 45 159 642.2 0 159 46 136 642.2 0 136 47 118 642.2 0 118 48 130 642.2 0 130 49 130 642.2 0 130 50 138 642.2 0 138 51 119 642.2 0 119 52 119 642.2 0 119 53 111 642.2 0 111 54 143 642.2 0 143 55 127 642.2 0 127 56 169 642.2 0 169 57 170 642.2 0 170 58 137 642.2 0 137 59 123 642.2 0 123 60 135 642.2 0 135 61 132 642.2 0 132 62 121 642.2 0 121 63 140 642.2 0 140 64 105 642.2 0 105 65 120 642.2 0 120 66 140 642.2 0 140 67 141 642.2 0 141 68 141 642.2 0 141 69 113 642.2 0 113 70 146 642.2 0 146 71 117 642.2 0 117 72 120 642.2 0 120 73 141 642.2 0 141 74 244 642.2 0 244 75 202 642.2 0 202 76 910 642.2 0 910 77 278 642.2 0 278 78 413 642.2 0 413 79 140 642.2 0 140 80 116 642.2 0 116 81 155 642.2 0 155 82 159 642.2 0 159 83 121 642.2 0 121 84 108 642.2 0 108 85 97 642.2 0 97 86 103 642.2 0 103 87 112 642.2 0 112 88 160 642.2 0 160 89 143 642.2 0 143 90 96 642.2 0 96 91 150 642.2 0 150 92 112 642.2 0 112 93 151 642.2 0 151 94 129 642.2 0 129 95 129 642.2 0 129 96 162 642.2 0 162 97 161 642.2 0 161 98 138 642.2 0 138 99 106 642.2 0 106 100 150 642.2 0 150 101 138 642.2 0 138 102 113 642.2 0 113 103 153 642.2 0 153 104 139 642.2 0 139 105 127 642.2 0 127 106 173 642.2 0 173 107 480 642.2 0 480 108 290 642.2 0 290 109 385 642.2 0 385 110 162 642.2 0 162 111 145 642.2 0 145 112 123 642.2 0 123 113 164 642.2 0 164 114 148 642.2 0 148 115 170 642.2 0 170 116 140 642.2 0 140 117 166 642.2 0 166 118 149 642.2 0 149 119 156 642.2 0 156 120 205 642.2 0 205 121 172 642.2 0 172 122 138 642.2 0 138 123 154 642.2 0 154 124 153 642.2 0 153 125 177 642.2 0 177 126 164 642.2 0 164 127 174 642.2 0 174 128 194 642.2 0 194 129 344 642.2 0 344 130 164 642.2 0 164 131 165 642.2 0 165 132 258 642.2 0 258 133 555 642.2 0 555 134 2241 642.2 0 2241 135 1661 642.2 0 1661 136 2326 642.2 0 2326 137 465 642.2 0 465 138 167 642.2 0 167 139 226 642.2 0 226 140 185 642.2 0 185 141 237 642.2 0 237 142 270 642.2 0 270 143 155 642.2 0 155 144 125 642.2 0 125 145 157 642.2 0 157 146 218 642.2 0 218 147 593 642.2 0 593 148 416 642.2 0 416 149 334 642.2 0 334 150 157 642.2 0 157 151 158 642.2 0 158 152 211 642.2 0 211 153 331 642.2 0 331 154 787 642.2 0 787 155 667 642.2 0 667 156 1017 642.2 0 1017 157 2266 642.2 0 2266 158 2000 642.2 0 2000 159 1514 642.2 0 1514 160 207 642.2 0 207 161 312 642.2 0 312 162 265 642.2 0 265 163 220 642.2 0 220 164 321 642.2 0 321 165 207 642.2 0 207 166 353 642.2 0 353 167 200 642.2 0 200 168 167 642.2 0 167 169 191 642.2 0 191 170 120 642.2 0 120 171 143 642.2 0 143 172 173 642.2 0 173 173 186 642.2 0 186 174 179 642.2 0 179 175 191 642.2 0 191 176 133 642.2 0 133 177 127 642.2 0 127 178 135 642.2 0 135 179 156 642.2 0 156 180 156 642.2 0 156 181 176 642.2 0 176 182 186 642.2 0 186 183 169 642.2 0 169 184 158 642.2 0 158 185 163 642.2 0 163 186 200 642.2 0 200 187 147 642.2 0 147 188 141 642.2 0 141 189 149 642.2 0 149 190 156 642.2 0 156 191 165 642.2 0 165 192 144 642.2 0 144 193 141 642.2 0 141 194 137 642.2 0 137 195 102 642.2 0 102 196 162 642.2 0 162 197 135 642.2 0 135 198 138 642.2 0 138 199 131 642.2 0 131 200 148 642.2 0 148 201 160 642.2 0 160 202 195 642.2 0 195 203 158 642.2 0 158 204 135 642.2 0 135 205 153 642.2 0 153 206 141 642.2 0 141 207 203 642.2 0 203 208 145 642.2 0 145 209 137 642.2 0 137 210 107 642.2 0 107 211 131 642.2 0 131 212 160 642.2 0 160 213 238 642.2 0 238 214 272 642.2 0 272 215 210 642.2 0 210 216 211 642.2 0 211 217 179 642.2 0 179 218 151 642.2 0 151 219 155 642.2 0 155 220 224 642.2 0 224 221 218 642.2 0 218 222 194 642.2 0 194 223 183 642.2 0 183 224 163 642.2 0 163 225 141 642.2 0 141 226 207 642.2 0 207 227 405 642.2 0 405 228 4026 642.2 0 4026 229 226 642.2 0 226 230 150 642.2 0 150 231 177 642.2 0 177 232 242 642.2 0 242 233 274 642.2 0 274 234 238 642.2 0 238 235 212 642.2 0 212 236 146 642.2 0 146 237 149 642.2 0 149 238 170 642.2 0 170 239 163 642.2 0 163 240 170 642.2 0 170 241 175 642.2 0 175 242 171 642.2 0 171 243 181 642.2 0 181 244 181 642.2 0 181 245 160 642.2 0 160 246 172 642.2 0 172 247 133 642.2 0 133 248 102 642.2 0 102 249 111 642.2 0 111 250 165 642.2 0 165 251 223 642.2 0 223 252 538 642.2 0 538 253 414 642.2 0 414 254 468 642.2 0 468 255 193 642.2 0 193 256 162 642.2 0 162 257 158 642.2 0 158 258 152 642.2 0 152 259 139 642.2 0 139 260 155 642.2 0 155 261 172 642.2 0 172 262 277 642.2 0 277 263 262 642.2 0 262 264 201 642.2 0 201 265 146 642.2 0 146 266 152 642.2 0 152 267 161 642.2 0 161 268 373 642.2 0 373 269 385 642.2 0 385 270 704 642.2 0 704 271 356 642.2 0 356 272 720 642.2 0 720 273 169 642.2 0 169 274 203 642.2 0 203 275 310 642.2 0 310 276 1209 642.2 0 1209 277 1411 642.2 0 1411 278 1172 642.2 0 1172 279 581 642.2 0 581 280 286 642.2 0 286 281 238 642.2 0 238 282 267 642.2 0 267 283 206 642.2 0 206 284 221 642.2 0 221 285 235 642.2 0 235 286 172 642.2 0 172 287 168 642.2 0 168 288 223 642.2 0 223 289 380 642.2 0 380 290 376 642.2 0 376 291 523 642.2 0 523 292 233 642.2 0 233 293 234 642.2 0 234 294 347 642.2 0 347 295 2981 642.2 0 2981 296 4402 642.2 0 4402 297 4845 642.2 0 4845 298 179 642.2 0 179 299 155 642.2 0 155 300 163 642.2 0 163 301 177 642.2 0 177 Finished in 2.00 s (40 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 49,429 Reads with adapters: 1,112 (2.2%) Reads that were too short: 26 (0.1%) Reads written (passing filters): 1,086 (2.2%) Total basepairs processed: 9,226,339 bp Total written (filtered): 290,391 bp (3.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1112 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 15 0.0 3 0 10 0 4 1 20 992 0.0 4 854 112 19 6 1 21 7 0.0 4 2 2 1 2 22 3 0.0 4 1 1 1 23 2 0.0 4 0 1 0 1 26 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 8 0.0 4 0 0 0 0 8 82 9 0.0 4 0 0 0 0 9 83 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 105 2 0.0 4 0 0 0 0 2 123 4 0.0 4 0 0 0 0 4 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 51 0.0 4 0 0 0 0 51 149 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (28 us/read; 2.17 M reads/minute). === Summary === Total reads processed: 1,086 Reads with adapters: 39 (3.6%) Reads written (passing filters): 1,086 (100.0%) Total basepairs processed: 290,391 bp Total written (filtered): 289,987 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 39 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 20.5% G: 71.8% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 17.0 0 3 4 2 4.2 0 2 10 1 0.0 1 0 1 11 22 0.0 1 1 21 12 10 0.0 1 0 10 15 1 0.0 1 0 1