This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.84 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 107,288 (4.1%) Reads that were too short: 52,221 (2.0%) Reads written (passing filters): 55,067 (2.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 10,431,136 bp (1.3%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 107288 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 166 642.2 0 166 7 2269 642.2 0 2269 8 179 642.2 0 179 9 189 642.2 0 189 10 218 642.2 0 218 11 226 642.2 0 226 12 156 642.2 0 156 13 232 642.2 0 232 14 223 642.2 0 223 15 169 642.2 0 169 16 202 642.2 0 202 17 165 642.2 0 165 18 188 642.2 0 188 19 178 642.2 0 178 20 185 642.2 0 185 21 172 642.2 0 172 22 162 642.2 0 162 23 184 642.2 0 184 24 183 642.2 0 183 25 182 642.2 0 182 26 184 642.2 0 184 27 159 642.2 0 159 28 191 642.2 0 191 29 158 642.2 0 158 30 125 642.2 0 125 31 168 642.2 0 168 32 173 642.2 0 173 33 160 642.2 0 160 34 189 642.2 0 189 35 208 642.2 0 208 36 164 642.2 0 164 37 131 642.2 0 131 38 164 642.2 0 164 39 165 642.2 0 165 40 153 642.2 0 153 41 158 642.2 0 158 42 223 642.2 0 223 43 178 642.2 0 178 44 170 642.2 0 170 45 188 642.2 0 188 46 166 642.2 0 166 47 163 642.2 0 163 48 155 642.2 0 155 49 149 642.2 0 149 50 169 642.2 0 169 51 160 642.2 0 160 52 141 642.2 0 141 53 153 642.2 0 153 54 164 642.2 0 164 55 171 642.2 0 171 56 180 642.2 0 180 57 208 642.2 0 208 58 171 642.2 0 171 59 152 642.2 0 152 60 203 642.2 0 203 61 174 642.2 0 174 62 199 642.2 0 199 63 159 642.2 0 159 64 172 642.2 0 172 65 157 642.2 0 157 66 170 642.2 0 170 67 191 642.2 0 191 68 172 642.2 0 172 69 163 642.2 0 163 70 184 642.2 0 184 71 177 642.2 0 177 72 189 642.2 0 189 73 204 642.2 0 204 74 246 642.2 0 246 75 215 642.2 0 215 76 877 642.2 0 877 77 259 642.2 0 259 78 350 642.2 0 350 79 192 642.2 0 192 80 193 642.2 0 193 81 214 642.2 0 214 82 220 642.2 0 220 83 158 642.2 0 158 84 183 642.2 0 183 85 193 642.2 0 193 86 153 642.2 0 153 87 173 642.2 0 173 88 207 642.2 0 207 89 197 642.2 0 197 90 184 642.2 0 184 91 208 642.2 0 208 92 181 642.2 0 181 93 213 642.2 0 213 94 162 642.2 0 162 95 206 642.2 0 206 96 199 642.2 0 199 97 211 642.2 0 211 98 179 642.2 0 179 99 178 642.2 0 178 100 194 642.2 0 194 101 149 642.2 0 149 102 173 642.2 0 173 103 233 642.2 0 233 104 191 642.2 0 191 105 161 642.2 0 161 106 238 642.2 0 238 107 307 642.2 0 307 108 222 642.2 0 222 109 271 642.2 0 271 110 202 642.2 0 202 111 242 642.2 0 242 112 178 642.2 0 178 113 236 642.2 0 236 114 221 642.2 0 221 115 215 642.2 0 215 116 203 642.2 0 203 117 216 642.2 0 216 118 204 642.2 0 204 119 214 642.2 0 214 120 274 642.2 0 274 121 256 642.2 0 256 122 203 642.2 0 203 123 227 642.2 0 227 124 210 642.2 0 210 125 198 642.2 0 198 126 244 642.2 0 244 127 241 642.2 0 241 128 217 642.2 0 217 129 356 642.2 0 356 130 233 642.2 0 233 131 224 642.2 0 224 132 300 642.2 0 300 133 545 642.2 0 545 134 2406 642.2 0 2406 135 1658 642.2 0 1658 136 2498 642.2 0 2498 137 459 642.2 0 459 138 214 642.2 0 214 139 255 642.2 0 255 140 225 642.2 0 225 141 214 642.2 0 214 142 219 642.2 0 219 143 203 642.2 0 203 144 170 642.2 0 170 145 206 642.2 0 206 146 237 642.2 0 237 147 373 642.2 0 373 148 320 642.2 0 320 149 274 642.2 0 274 150 210 642.2 0 210 151 189 642.2 0 189 152 232 642.2 0 232 153 289 642.2 0 289 154 803 642.2 0 803 155 688 642.2 0 688 156 1063 642.2 0 1063 157 2393 642.2 0 2393 158 2436 642.2 0 2436 159 1492 642.2 0 1492 160 193 642.2 0 193 161 271 642.2 0 271 162 189 642.2 0 189 163 197 642.2 0 197 164 235 642.2 0 235 165 208 642.2 0 208 166 223 642.2 0 223 167 212 642.2 0 212 168 187 642.2 0 187 169 186 642.2 0 186 170 146 642.2 0 146 171 169 642.2 0 169 172 203 642.2 0 203 173 173 642.2 0 173 174 206 642.2 0 206 175 161 642.2 0 161 176 165 642.2 0 165 177 179 642.2 0 179 178 189 642.2 0 189 179 160 642.2 0 160 180 158 642.2 0 158 181 153 642.2 0 153 182 185 642.2 0 185 183 163 642.2 0 163 184 166 642.2 0 166 185 137 642.2 0 137 186 236 642.2 0 236 187 148 642.2 0 148 188 166 642.2 0 166 189 162 642.2 0 162 190 156 642.2 0 156 191 179 642.2 0 179 192 176 642.2 0 176 193 177 642.2 0 177 194 121 642.2 0 121 195 125 642.2 0 125 196 160 642.2 0 160 197 167 642.2 0 167 198 117 642.2 0 117 199 150 642.2 0 150 200 136 642.2 0 136 201 149 642.2 0 149 202 182 642.2 0 182 203 139 642.2 0 139 204 150 642.2 0 150 205 173 642.2 0 173 206 159 642.2 0 159 207 176 642.2 0 176 208 160 642.2 0 160 209 137 642.2 0 137 210 150 642.2 0 150 211 146 642.2 0 146 212 161 642.2 0 161 213 169 642.2 0 169 214 230 642.2 0 230 215 168 642.2 0 168 216 200 642.2 0 200 217 173 642.2 0 173 218 140 642.2 0 140 219 174 642.2 0 174 220 206 642.2 0 206 221 192 642.2 0 192 222 181 642.2 0 181 223 186 642.2 0 186 224 146 642.2 0 146 225 165 642.2 0 165 226 207 642.2 0 207 227 445 642.2 0 445 228 4968 642.2 0 4968 229 313 642.2 0 313 230 146 642.2 0 146 231 164 642.2 0 164 232 170 642.2 0 170 233 160 642.2 0 160 234 154 642.2 0 154 235 161 642.2 0 161 236 130 642.2 0 130 237 165 642.2 0 165 238 186 642.2 0 186 239 146 642.2 0 146 240 161 642.2 0 161 241 137 642.2 0 137 242 173 642.2 0 173 243 162 642.2 0 162 244 177 642.2 0 177 245 150 642.2 0 150 246 131 642.2 0 131 247 159 642.2 0 159 248 141 642.2 0 141 249 120 642.2 0 120 250 160 642.2 0 160 251 179 642.2 0 179 252 240 642.2 0 240 253 291 642.2 0 291 254 279 642.2 0 279 255 142 642.2 0 142 256 154 642.2 0 154 257 139 642.2 0 139 258 155 642.2 0 155 259 128 642.2 0 128 260 143 642.2 0 143 261 139 642.2 0 139 262 189 642.2 0 189 263 139 642.2 0 139 264 154 642.2 0 154 265 139 642.2 0 139 266 120 642.2 0 120 267 136 642.2 0 136 268 255 642.2 0 255 269 229 642.2 0 229 270 654 642.2 0 654 271 345 642.2 0 345 272 825 642.2 0 825 273 172 642.2 0 172 274 214 642.2 0 214 275 253 642.2 0 253 276 1195 642.2 0 1195 277 1469 642.2 0 1469 278 1209 642.2 0 1209 279 560 642.2 0 560 280 221 642.2 0 221 281 202 642.2 0 202 282 269 642.2 0 269 283 176 642.2 0 176 284 190 642.2 0 190 285 255 642.2 0 255 286 150 642.2 0 150 287 165 642.2 0 165 288 231 642.2 0 231 289 258 642.2 0 258 290 434 642.2 0 434 291 473 642.2 0 473 292 288 642.2 0 288 293 305 642.2 0 305 294 416 642.2 0 416 295 5213 642.2 0 5213 296 8678 642.2 0 8678 297 10153 642.2 0 10153 298 175 642.2 0 175 299 146 642.2 0 146 300 168 642.2 0 168 301 160 642.2 0 160 Finished in 2.00 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 55,067 Reads with adapters: 1,070 (1.9%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 1,050 (1.9%) Total basepairs processed: 10,431,136 bp Total written (filtered): 285,741 bp (2.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 1070 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 2 0.0 3 0 1 0 1 17 1009 0.0 3 845 150 14 19 4 0.0 3 2 0 2 20 2 0.0 3 0 0 0 2 24 1 0.0 3 1 27 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 32 7 0.0 3 0 0 0 7 35 4 0.0 3 0 0 0 4 36 1 0.0 3 0 0 0 1 58 12 0.0 3 0 0 0 12 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 1 128 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 1 1 164 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (29 us/read; 2.10 M reads/minute). === Summary === Total reads processed: 1,050 Reads with adapters: 2 (0.2%) Reads written (passing filters): 1,050 (100.0%) Total basepairs processed: 285,741 bp Total written (filtered): 285,733 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 0.0% G: 100.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 16.4 0 1 5 1 1.0 0 1