This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.81 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 86,614 (3.3%) Reads that were too short: 23,395 (0.9%) Reads written (passing filters): 63,219 (2.4%) Total basepairs processed: 791,719,999 bp Total written (filtered): 11,919,252 bp (1.5%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 86614 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 163 642.2 0 163 7 260 642.2 0 260 8 6937 642.2 0 6937 9 5348 642.2 0 5348 10 16 642.2 0 16 11 25 642.2 0 25 12 7 642.2 0 7 13 42 642.2 0 42 14 187 642.2 0 187 15 335 642.2 0 335 16 51 642.2 0 51 17 24 642.2 0 24 18 63 642.2 0 63 19 54 642.2 0 54 20 43 642.2 0 43 21 14 642.2 0 14 22 3 642.2 0 3 23 7 642.2 0 7 24 12 642.2 0 12 25 6 642.2 0 6 26 6 642.2 0 6 27 26 642.2 0 26 28 22 642.2 0 22 29 12 642.2 0 12 30 10 642.2 0 10 31 11 642.2 0 11 32 30 642.2 0 30 33 12 642.2 0 12 34 17 642.2 0 17 35 14 642.2 0 14 36 10 642.2 0 10 37 10 642.2 0 10 38 12 642.2 0 12 39 13 642.2 0 13 40 13 642.2 0 13 41 4 642.2 0 4 42 19 642.2 0 19 43 142 642.2 0 142 44 419 642.2 0 419 45 484 642.2 0 484 46 166 642.2 0 166 47 34 642.2 0 34 48 6 642.2 0 6 49 9 642.2 0 9 50 90 642.2 0 90 51 21 642.2 0 21 52 54 642.2 0 54 53 34 642.2 0 34 54 11 642.2 0 11 55 114 642.2 0 114 56 16 642.2 0 16 57 22 642.2 0 22 58 45 642.2 0 45 59 22 642.2 0 22 60 49 642.2 0 49 61 25 642.2 0 25 62 13 642.2 0 13 63 11 642.2 0 11 64 70 642.2 0 70 65 21 642.2 0 21 66 20 642.2 0 20 67 65 642.2 0 65 68 62 642.2 0 62 69 55 642.2 0 55 70 100 642.2 0 100 71 31 642.2 0 31 72 57 642.2 0 57 73 56 642.2 0 56 74 69 642.2 0 69 75 69 642.2 0 69 76 377 642.2 0 377 77 2364 642.2 0 2364 78 1988 642.2 0 1988 79 1621 642.2 0 1621 80 36 642.2 0 36 81 189 642.2 0 189 82 21 642.2 0 21 83 18 642.2 0 18 84 64 642.2 0 64 85 28 642.2 0 28 86 35 642.2 0 35 87 8 642.2 0 8 88 15 642.2 0 15 89 18 642.2 0 18 90 43 642.2 0 43 91 65 642.2 0 65 92 62 642.2 0 62 93 85 642.2 0 85 94 52 642.2 0 52 95 40 642.2 0 40 96 29 642.2 0 29 97 22 642.2 0 22 98 16 642.2 0 16 99 58 642.2 0 58 100 95 642.2 0 95 101 156 642.2 0 156 102 28 642.2 0 28 103 143 642.2 0 143 104 71 642.2 0 71 105 27 642.2 0 27 106 46 642.2 0 46 107 56 642.2 0 56 108 39 642.2 0 39 109 54 642.2 0 54 110 64 642.2 0 64 111 93 642.2 0 93 112 112 642.2 0 112 113 209 642.2 0 209 114 3630 642.2 0 3630 115 492 642.2 0 492 116 8895 642.2 0 8895 117 91 642.2 0 91 118 30 642.2 0 30 119 47 642.2 0 47 120 39 642.2 0 39 121 20 642.2 0 20 122 123 642.2 0 123 123 34 642.2 0 34 124 33 642.2 0 33 125 32 642.2 0 32 126 62 642.2 0 62 127 62 642.2 0 62 128 36 642.2 0 36 129 27 642.2 0 27 130 29 642.2 0 29 131 16 642.2 0 16 132 20 642.2 0 20 133 39 642.2 0 39 134 43 642.2 0 43 135 121 642.2 0 121 136 174 642.2 0 174 137 127 642.2 0 127 138 46 642.2 0 46 139 125 642.2 0 125 140 54 642.2 0 54 141 31 642.2 0 31 142 42 642.2 0 42 143 40 642.2 0 40 144 52 642.2 0 52 145 19 642.2 0 19 146 18 642.2 0 18 147 17 642.2 0 17 148 23 642.2 0 23 149 27 642.2 0 27 150 55 642.2 0 55 151 291 642.2 0 291 152 50 642.2 0 50 153 19 642.2 0 19 154 38 642.2 0 38 155 50 642.2 0 50 156 81 642.2 0 81 157 22 642.2 0 22 158 86 642.2 0 86 159 18 642.2 0 18 160 34 642.2 0 34 161 35 642.2 0 35 162 26 642.2 0 26 163 65 642.2 0 65 164 128 642.2 0 128 165 178 642.2 0 178 166 65 642.2 0 65 167 167 642.2 0 167 168 45 642.2 0 45 169 94 642.2 0 94 170 34 642.2 0 34 171 34 642.2 0 34 172 106 642.2 0 106 173 697 642.2 0 697 174 1170 642.2 0 1170 175 1202 642.2 0 1202 176 121 642.2 0 121 177 33 642.2 0 33 178 53 642.2 0 53 179 135 642.2 0 135 180 1012 642.2 0 1012 181 510 642.2 0 510 182 476 642.2 0 476 183 78 642.2 0 78 184 51 642.2 0 51 185 155 642.2 0 155 186 74 642.2 0 74 187 92 642.2 0 92 188 104 642.2 0 104 189 159 642.2 0 159 190 960 642.2 0 960 191 6979 642.2 0 6979 192 4115 642.2 0 4115 193 2228 642.2 0 2228 194 1160 642.2 0 1160 195 65 642.2 0 65 196 47 642.2 0 47 197 37 642.2 0 37 198 62 642.2 0 62 199 183 642.2 0 183 200 33 642.2 0 33 201 44 642.2 0 44 202 269 642.2 0 269 203 987 642.2 0 987 204 637 642.2 0 637 205 265 642.2 0 265 206 35 642.2 0 35 207 312 642.2 0 312 208 135 642.2 0 135 209 421 642.2 0 421 210 196 642.2 0 196 211 83 642.2 0 83 212 16 642.2 0 16 213 20 642.2 0 20 214 17 642.2 0 17 215 21 642.2 0 21 216 63 642.2 0 63 217 66 642.2 0 66 218 70 642.2 0 70 219 34 642.2 0 34 220 46 642.2 0 46 221 27 642.2 0 27 222 45 642.2 0 45 223 29 642.2 0 29 224 19 642.2 0 19 225 38 642.2 0 38 226 38 642.2 0 38 227 40 642.2 0 40 228 18 642.2 0 18 229 33 642.2 0 33 230 53 642.2 0 53 231 50 642.2 0 50 232 45 642.2 0 45 233 36 642.2 0 36 234 94 642.2 0 94 235 54 642.2 0 54 236 55 642.2 0 55 237 39 642.2 0 39 238 33 642.2 0 33 239 20 642.2 0 20 240 47 642.2 0 47 241 27 642.2 0 27 242 35 642.2 0 35 243 44 642.2 0 44 244 36 642.2 0 36 245 60 642.2 0 60 246 43 642.2 0 43 247 27 642.2 0 27 248 39 642.2 0 39 249 35 642.2 0 35 250 40 642.2 0 40 251 133 642.2 0 133 252 387 642.2 0 387 253 354 642.2 0 354 254 464 642.2 0 464 255 100 642.2 0 100 256 51 642.2 0 51 257 63 642.2 0 63 258 352 642.2 0 352 259 360 642.2 0 360 260 1746 642.2 0 1746 261 912 642.2 0 912 262 625 642.2 0 625 263 386 642.2 0 386 264 459 642.2 0 459 265 668 642.2 0 668 266 386 642.2 0 386 267 87 642.2 0 87 268 44 642.2 0 44 269 30 642.2 0 30 270 45 642.2 0 45 271 65 642.2 0 65 272 244 642.2 0 244 273 93 642.2 0 93 274 83 642.2 0 83 275 63 642.2 0 63 276 37 642.2 0 37 277 89 642.2 0 89 278 84 642.2 0 84 279 133 642.2 0 133 280 121 642.2 0 121 281 118 642.2 0 118 282 73 642.2 0 73 283 228 642.2 0 228 284 1595 642.2 0 1595 285 2623 642.2 0 2623 286 2647 642.2 0 2647 287 776 642.2 0 776 288 52 642.2 0 52 289 78 642.2 0 78 290 55 642.2 0 55 291 58 642.2 0 58 292 80 642.2 0 80 293 44 642.2 0 44 294 91 642.2 0 91 295 482 642.2 0 482 296 176 642.2 0 176 297 323 642.2 0 323 298 97 642.2 0 97 299 51 642.2 0 51 300 45 642.2 0 45 301 147 642.2 0 147 Finished in 2.63 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 63,219 Reads with adapters: 5,957 (9.4%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 5,931 (9.4%) Total basepairs processed: 11,919,252 bp Total written (filtered): 1,611,886 bp (13.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5957 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 3 0.0 3 0 0 3 19 116 0.0 3 0 92 14 7 3 20 5752 0.0 4 5258 381 82 24 7 21 19 0.0 4 7 10 1 1 22 4 0.0 4 0 0 3 0 1 23 16 0.0 4 0 0 0 6 10 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 1 50 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 91 3 0.0 4 0 0 0 0 3 97 1 0.0 4 0 0 1 104 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 108 2 0.0 4 0 0 0 0 2 109 1 0.0 4 0 0 0 0 1 121 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 149 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 157 4 0.0 4 0 0 0 0 4 158 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 186 2 0.0 4 0 0 0 0 2 187 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.21 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 5,931 Reads with adapters: 1,379 (23.3%) Reads written (passing filters): 5,931 (100.0%) Total basepairs processed: 1,611,886 bp Total written (filtered): 1,597,553 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1379 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 12.1% G: 82.4% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 92.7 0 4 4 2 23.2 0 2 5 1 5.8 0 1 9 197 0.0 0 25 172 10 952 0.0 1 75 877 11 215 0.0 1 9 206 12 2 0.0 1 0 2 13 2 0.0 1 0 2 150 4 0.0 1 3 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.