This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.35 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 71,380 (2.7%) Reads that were too short: 10,729 (0.4%) Reads written (passing filters): 60,651 (2.3%) Total basepairs processed: 791,719,999 bp Total written (filtered): 11,494,835 bp (1.5%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 71380 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 162 642.2 0 162 7 226 642.2 0 226 8 6617 642.2 0 6617 9 6284 642.2 0 6284 10 4 642.2 0 4 11 3 642.2 0 3 12 3 642.2 0 3 13 4 642.2 0 4 14 4 642.2 0 4 15 8 642.2 0 8 16 1 642.2 0 1 17 6 642.2 0 6 18 16 642.2 0 16 19 7 642.2 0 7 20 14 642.2 0 14 21 12 642.2 0 12 22 4 642.2 0 4 23 5 642.2 0 5 24 9 642.2 0 9 25 9 642.2 0 9 26 1 642.2 0 1 27 5 642.2 0 5 28 2 642.2 0 2 29 4 642.2 0 4 30 9 642.2 0 9 31 9 642.2 0 9 32 36 642.2 0 36 33 11 642.2 0 11 34 8 642.2 0 8 35 7 642.2 0 7 36 2 642.2 0 2 37 8 642.2 0 8 38 6 642.2 0 6 39 8 642.2 0 8 40 6 642.2 0 6 41 9 642.2 0 9 42 14 642.2 0 14 43 17 642.2 0 17 44 36 642.2 0 36 45 65 642.2 0 65 46 27 642.2 0 27 47 31 642.2 0 31 48 7 642.2 0 7 49 14 642.2 0 14 50 87 642.2 0 87 51 9 642.2 0 9 52 59 642.2 0 59 53 20 642.2 0 20 54 12 642.2 0 12 55 138 642.2 0 138 56 11 642.2 0 11 57 19 642.2 0 19 58 45 642.2 0 45 59 16 642.2 0 16 60 22 642.2 0 22 61 6 642.2 0 6 62 17 642.2 0 17 63 9 642.2 0 9 64 62 642.2 0 62 65 19 642.2 0 19 66 18 642.2 0 18 67 25 642.2 0 25 68 10 642.2 0 10 69 37 642.2 0 37 70 56 642.2 0 56 71 20 642.2 0 20 72 48 642.2 0 48 73 36 642.2 0 36 74 41 642.2 0 41 75 55 642.2 0 55 76 223 642.2 0 223 77 2702 642.2 0 2702 78 2286 642.2 0 2286 79 1685 642.2 0 1685 80 18 642.2 0 18 81 181 642.2 0 181 82 33 642.2 0 33 83 10 642.2 0 10 84 25 642.2 0 25 85 7 642.2 0 7 86 10 642.2 0 10 87 8 642.2 0 8 88 13 642.2 0 13 89 22 642.2 0 22 90 35 642.2 0 35 91 33 642.2 0 33 92 26 642.2 0 26 93 46 642.2 0 46 94 45 642.2 0 45 95 16 642.2 0 16 96 16 642.2 0 16 97 20 642.2 0 20 98 29 642.2 0 29 99 50 642.2 0 50 100 94 642.2 0 94 101 174 642.2 0 174 102 33 642.2 0 33 103 134 642.2 0 134 104 97 642.2 0 97 105 37 642.2 0 37 106 49 642.2 0 49 107 52 642.2 0 52 108 48 642.2 0 48 109 54 642.2 0 54 110 57 642.2 0 57 111 86 642.2 0 86 112 101 642.2 0 101 113 173 642.2 0 173 114 4357 642.2 0 4357 115 380 642.2 0 380 116 10398 642.2 0 10398 117 78 642.2 0 78 118 24 642.2 0 24 119 36 642.2 0 36 120 37 642.2 0 37 121 17 642.2 0 17 122 122 642.2 0 122 123 18 642.2 0 18 124 31 642.2 0 31 125 6 642.2 0 6 126 11 642.2 0 11 127 16 642.2 0 16 128 10 642.2 0 10 129 3 642.2 0 3 130 14 642.2 0 14 131 10 642.2 0 10 132 6 642.2 0 6 133 34 642.2 0 34 134 17 642.2 0 17 135 57 642.2 0 57 136 103 642.2 0 103 137 49 642.2 0 49 138 20 642.2 0 20 139 137 642.2 0 137 140 42 642.2 0 42 141 12 642.2 0 12 142 11 642.2 0 11 143 56 642.2 0 56 144 15 642.2 0 15 145 11 642.2 0 11 146 12 642.2 0 12 147 9 642.2 0 9 148 9 642.2 0 9 149 13 642.2 0 13 150 28 642.2 0 28 151 305 642.2 0 305 152 46 642.2 0 46 153 9 642.2 0 9 154 22 642.2 0 22 155 20 642.2 0 20 156 85 642.2 0 85 157 14 642.2 0 14 158 82 642.2 0 82 159 15 642.2 0 15 160 10 642.2 0 10 161 20 642.2 0 20 162 10 642.2 0 10 163 30 642.2 0 30 164 48 642.2 0 48 165 103 642.2 0 103 166 10 642.2 0 10 167 153 642.2 0 153 168 16 642.2 0 16 169 89 642.2 0 89 170 26 642.2 0 26 171 16 642.2 0 16 172 41 642.2 0 41 173 206 642.2 0 206 174 427 642.2 0 427 175 440 642.2 0 440 176 99 642.2 0 99 177 14 642.2 0 14 178 36 642.2 0 36 179 74 642.2 0 74 180 489 642.2 0 489 181 221 642.2 0 221 182 183 642.2 0 183 183 70 642.2 0 70 184 23 642.2 0 23 185 42 642.2 0 42 186 49 642.2 0 49 187 68 642.2 0 68 188 92 642.2 0 92 189 165 642.2 0 165 190 865 642.2 0 865 191 8577 642.2 0 8577 192 4789 642.2 0 4789 193 2037 642.2 0 2037 194 621 642.2 0 621 195 23 642.2 0 23 196 55 642.2 0 55 197 12 642.2 0 12 198 33 642.2 0 33 199 162 642.2 0 162 200 13 642.2 0 13 201 22 642.2 0 22 202 192 642.2 0 192 203 355 642.2 0 355 204 209 642.2 0 209 205 138 642.2 0 138 206 23 642.2 0 23 207 356 642.2 0 356 208 25 642.2 0 25 209 43 642.2 0 43 210 24 642.2 0 24 211 18 642.2 0 18 212 6 642.2 0 6 213 8 642.2 0 8 214 4 642.2 0 4 215 5 642.2 0 5 216 14 642.2 0 14 217 20 642.2 0 20 218 12 642.2 0 12 219 8 642.2 0 8 220 7 642.2 0 7 221 7 642.2 0 7 222 6 642.2 0 6 223 4 642.2 0 4 224 13 642.2 0 13 225 8 642.2 0 8 226 8 642.2 0 8 227 11 642.2 0 11 228 14 642.2 0 14 229 8 642.2 0 8 230 53 642.2 0 53 231 18 642.2 0 18 232 10 642.2 0 10 233 16 642.2 0 16 234 35 642.2 0 35 235 16 642.2 0 16 236 19 642.2 0 19 237 19 642.2 0 19 238 14 642.2 0 14 239 30 642.2 0 30 240 36 642.2 0 36 241 16 642.2 0 16 242 9 642.2 0 9 243 9 642.2 0 9 244 9 642.2 0 9 245 33 642.2 0 33 246 49 642.2 0 49 247 14 642.2 0 14 248 19 642.2 0 19 249 10 642.2 0 10 250 22 642.2 0 22 251 53 642.2 0 53 252 126 642.2 0 126 253 131 642.2 0 131 254 179 642.2 0 179 255 30 642.2 0 30 256 30 642.2 0 30 257 33 642.2 0 33 258 156 642.2 0 156 259 297 642.2 0 297 260 890 642.2 0 890 261 514 642.2 0 514 262 224 642.2 0 224 263 102 642.2 0 102 264 206 642.2 0 206 265 229 642.2 0 229 266 195 642.2 0 195 267 21 642.2 0 21 268 16 642.2 0 16 269 17 642.2 0 17 270 22 642.2 0 22 271 31 642.2 0 31 272 111 642.2 0 111 273 34 642.2 0 34 274 32 642.2 0 32 275 22 642.2 0 22 276 19 642.2 0 19 277 28 642.2 0 28 278 27 642.2 0 27 279 45 642.2 0 45 280 28 642.2 0 28 281 31 642.2 0 31 282 37 642.2 0 37 283 49 642.2 0 49 284 615 642.2 0 615 285 1222 642.2 0 1222 286 1098 642.2 0 1098 287 1167 642.2 0 1167 288 30 642.2 0 30 289 39 642.2 0 39 290 25 642.2 0 25 291 25 642.2 0 25 292 20 642.2 0 20 293 13 642.2 0 13 294 22 642.2 0 22 295 144 642.2 0 144 296 60 642.2 0 60 297 161 642.2 0 161 298 23 642.2 0 23 299 12 642.2 0 12 300 19 642.2 0 19 301 67 642.2 0 67 Finished in 2.31 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 60,651 Reads with adapters: 6,567 (10.8%) Reads that were too short: 38 (0.1%) Reads written (passing filters): 6,529 (10.8%) Total basepairs processed: 11,494,835 bp Total written (filtered): 1,778,908 bp (15.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6567 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 58 0.0 3 0 55 3 17 6216 0.0 3 6094 118 4 18 38 0.0 3 2 17 2 17 19 5 0.0 3 0 1 2 2 20 70 0.0 3 63 3 0 4 21 5 0.0 3 0 1 0 4 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 54 6 0.0 3 0 0 0 6 56 1 0.0 3 0 0 0 1 57 75 0.0 3 0 0 0 75 58 18 0.0 3 0 0 0 18 60 9 0.0 3 0 0 1 8 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 75 1 0.0 3 1 76 1 0.0 3 0 0 0 1 81 4 0.0 3 2 2 83 4 0.0 3 0 0 0 4 89 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 111 2 0.0 3 1 0 0 1 118 1 0.0 3 0 0 0 1 148 2 0.0 3 0 1 0 1 150 1 0.0 3 0 0 1 151 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 168 1 0.0 3 0 0 0 1 169 5 0.0 3 0 0 0 5 170 1 0.0 3 0 0 0 1 172 8 0.0 3 0 0 0 8 173 2 0.0 3 0 0 2 181 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 6,529 Reads with adapters: 28 (0.4%) Reads written (passing filters): 6,529 (100.0%) Total basepairs processed: 1,778,908 bp Total written (filtered): 1,778,784 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 28 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.4% C: 14.3% G: 35.7% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18 102.0 0 18 4 5 25.5 0 5 6 1 1.6 0 1 11 4 0.0 1 0 4