This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.78 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 91,423 (3.5%) Reads that were too short: 14,009 (0.5%) Reads written (passing filters): 77,414 (2.9%) Total basepairs processed: 791,719,999 bp Total written (filtered): 21,378,302 bp (2.7%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 91423 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 670 642.2 0 670 7 34994 642.2 0 34994 8 28478 642.2 0 28478 9 28 642.2 0 28 10 22 642.2 0 22 11 7 642.2 0 7 12 10 642.2 0 10 13 24 642.2 0 24 14 27 642.2 0 27 15 30 642.2 0 30 16 18 642.2 0 18 17 16 642.2 0 16 18 84 642.2 0 84 19 67 642.2 0 67 20 70 642.2 0 70 21 15 642.2 0 15 22 20 642.2 0 20 23 17 642.2 0 17 24 14 642.2 0 14 25 24 642.2 0 24 26 32 642.2 0 32 27 42 642.2 0 42 28 38 642.2 0 38 29 32 642.2 0 32 30 21 642.2 0 21 31 10 642.2 0 10 32 15 642.2 0 15 33 47 642.2 0 47 34 33 642.2 0 33 35 57 642.2 0 57 36 179 642.2 0 179 37 162 642.2 0 162 38 567 642.2 0 567 39 23 642.2 0 23 40 33 642.2 0 33 41 41 642.2 0 41 42 24 642.2 0 24 43 396 642.2 0 396 44 450 642.2 0 450 45 969 642.2 0 969 46 34 642.2 0 34 47 20 642.2 0 20 48 23 642.2 0 23 49 31 642.2 0 31 50 30 642.2 0 30 51 28 642.2 0 28 52 30 642.2 0 30 53 24 642.2 0 24 54 23 642.2 0 23 55 30 642.2 0 30 56 38 642.2 0 38 57 47 642.2 0 47 58 26 642.2 0 26 59 21 642.2 0 21 60 22 642.2 0 22 61 28 642.2 0 28 62 14 642.2 0 14 63 26 642.2 0 26 64 45 642.2 0 45 65 15 642.2 0 15 66 21 642.2 0 21 67 22 642.2 0 22 68 33 642.2 0 33 69 33 642.2 0 33 70 18 642.2 0 18 71 33 642.2 0 33 72 33 642.2 0 33 73 40 642.2 0 40 74 30 642.2 0 30 75 35 642.2 0 35 76 32 642.2 0 32 77 36 642.2 0 36 78 34 642.2 0 34 79 41 642.2 0 41 80 37 642.2 0 37 81 24 642.2 0 24 82 40 642.2 0 40 83 27 642.2 0 27 84 27 642.2 0 27 85 22 642.2 0 22 86 30 642.2 0 30 87 32 642.2 0 32 88 28 642.2 0 28 89 39 642.2 0 39 90 32 642.2 0 32 91 24 642.2 0 24 92 27 642.2 0 27 93 31 642.2 0 31 94 32 642.2 0 32 95 66 642.2 0 66 96 67 642.2 0 67 97 38 642.2 0 38 98 20 642.2 0 20 99 18 642.2 0 18 100 27 642.2 0 27 101 30 642.2 0 30 102 20 642.2 0 20 103 20 642.2 0 20 104 77 642.2 0 77 105 36 642.2 0 36 106 20 642.2 0 20 107 28 642.2 0 28 108 36 642.2 0 36 109 24 642.2 0 24 110 28 642.2 0 28 111 21 642.2 0 21 112 33 642.2 0 33 113 32 642.2 0 32 114 76 642.2 0 76 115 49 642.2 0 49 116 24 642.2 0 24 117 21 642.2 0 21 118 28 642.2 0 28 119 36 642.2 0 36 120 47 642.2 0 47 121 44 642.2 0 44 122 63 642.2 0 63 123 42 642.2 0 42 124 25 642.2 0 25 125 89 642.2 0 89 126 123 642.2 0 123 127 85 642.2 0 85 128 217 642.2 0 217 129 104 642.2 0 104 130 95 642.2 0 95 131 167 642.2 0 167 132 196 642.2 0 196 133 127 642.2 0 127 134 85 642.2 0 85 135 568 642.2 0 568 136 296 642.2 0 296 137 385 642.2 0 385 138 43 642.2 0 43 139 49 642.2 0 49 140 171 642.2 0 171 141 154 642.2 0 154 142 440 642.2 0 440 143 127 642.2 0 127 144 42 642.2 0 42 145 66 642.2 0 66 146 36 642.2 0 36 147 74 642.2 0 74 148 136 642.2 0 136 149 140 642.2 0 140 150 102 642.2 0 102 151 56 642.2 0 56 152 67 642.2 0 67 153 37 642.2 0 37 154 49 642.2 0 49 155 51 642.2 0 51 156 47 642.2 0 47 157 45 642.2 0 45 158 25 642.2 0 25 159 52 642.2 0 52 160 63 642.2 0 63 161 64 642.2 0 64 162 39 642.2 0 39 163 45 642.2 0 45 164 65 642.2 0 65 165 54 642.2 0 54 166 58 642.2 0 58 167 78 642.2 0 78 168 68 642.2 0 68 169 37 642.2 0 37 170 36 642.2 0 36 171 52 642.2 0 52 172 36 642.2 0 36 173 54 642.2 0 54 174 35 642.2 0 35 175 72 642.2 0 72 176 65 642.2 0 65 177 36 642.2 0 36 178 54 642.2 0 54 179 39 642.2 0 39 180 72 642.2 0 72 181 54 642.2 0 54 182 71 642.2 0 71 183 75 642.2 0 75 184 60 642.2 0 60 185 71 642.2 0 71 186 70 642.2 0 70 187 68 642.2 0 68 188 175 642.2 0 175 189 94 642.2 0 94 190 46 642.2 0 46 191 63 642.2 0 63 192 48 642.2 0 48 193 52 642.2 0 52 194 89 642.2 0 89 195 74 642.2 0 74 196 69 642.2 0 69 197 41 642.2 0 41 198 33 642.2 0 33 199 40 642.2 0 40 200 47 642.2 0 47 201 56 642.2 0 56 202 73 642.2 0 73 203 43 642.2 0 43 204 32 642.2 0 32 205 36 642.2 0 36 206 28 642.2 0 28 207 104 642.2 0 104 208 147 642.2 0 147 209 200 642.2 0 200 210 109 642.2 0 109 211 491 642.2 0 491 212 76 642.2 0 76 213 105 642.2 0 105 214 265 642.2 0 265 215 213 642.2 0 213 216 185 642.2 0 185 217 114 642.2 0 114 218 47 642.2 0 47 219 64 642.2 0 64 220 77 642.2 0 77 221 92 642.2 0 92 222 86 642.2 0 86 223 47 642.2 0 47 224 74 642.2 0 74 225 90 642.2 0 90 226 87 642.2 0 87 227 51 642.2 0 51 228 96 642.2 0 96 229 87 642.2 0 87 230 142 642.2 0 142 231 60 642.2 0 60 232 98 642.2 0 98 233 164 642.2 0 164 234 184 642.2 0 184 235 200 642.2 0 200 236 88 642.2 0 88 237 48 642.2 0 48 238 52 642.2 0 52 239 62 642.2 0 62 240 61 642.2 0 61 241 57 642.2 0 57 242 58 642.2 0 58 243 77 642.2 0 77 244 87 642.2 0 87 245 73 642.2 0 73 246 38 642.2 0 38 247 38 642.2 0 38 248 48 642.2 0 48 249 41 642.2 0 41 250 34 642.2 0 34 251 54 642.2 0 54 252 61 642.2 0 61 253 74 642.2 0 74 254 72 642.2 0 72 255 79 642.2 0 79 256 59 642.2 0 59 257 83 642.2 0 83 258 86 642.2 0 86 259 65 642.2 0 65 260 104 642.2 0 104 261 86 642.2 0 86 262 87 642.2 0 87 263 96 642.2 0 96 264 81 642.2 0 81 265 79 642.2 0 79 266 91 642.2 0 91 267 73 642.2 0 73 268 199 642.2 0 199 269 598 642.2 0 598 270 337 642.2 0 337 271 516 642.2 0 516 272 161 642.2 0 161 273 327 642.2 0 327 274 209 642.2 0 209 275 154 642.2 0 154 276 122 642.2 0 122 277 97 642.2 0 97 278 73 642.2 0 73 279 105 642.2 0 105 280 104 642.2 0 104 281 130 642.2 0 130 282 96 642.2 0 96 283 150 642.2 0 150 284 143 642.2 0 143 285 75 642.2 0 75 286 73 642.2 0 73 287 76 642.2 0 76 288 90 642.2 0 90 289 101 642.2 0 101 290 109 642.2 0 109 291 101 642.2 0 101 292 125 642.2 0 125 293 137 642.2 0 137 294 569 642.2 0 569 295 572 642.2 0 572 296 902 642.2 0 902 297 340 642.2 0 340 298 352 642.2 0 352 299 108 642.2 0 108 300 145 642.2 0 145 301 454 642.2 0 454 Finished in 4.17 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 77,414 Reads with adapters: 29,720 (38.4%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 29,682 (38.3%) Total basepairs processed: 21,378,302 bp Total written (filtered): 8,100,829 bp (37.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29720 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 21 0.0 3 0 0 12 4 5 19 506 0.0 3 0 378 64 44 20 20 28921 0.0 4 26303 1976 473 123 46 21 48 0.0 4 7 39 1 1 22 10 0.0 4 1 0 4 3 2 23 137 0.0 4 0 0 0 92 45 24 2 0.0 4 1 0 0 0 1 34 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 91 10 0.0 4 0 0 0 0 10 93 1 0.0 4 0 0 0 0 1 98 4 0.0 4 0 0 0 0 4 100 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 127 11 0.0 4 0 0 0 0 11 132 1 0.0 4 0 1 135 3 0.0 4 1 0 0 0 2 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 1 215 1 0.0 4 0 0 0 1 218 1 0.0 4 0 0 0 0 1 227 3 0.0 4 0 0 0 0 3 228 5 0.0 4 0 0 0 0 5 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 259 2 0.0 4 0 0 0 1 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 276 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.08 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 29,682 Reads with adapters: 6,361 (21.4%) Reads written (passing filters): 29,682 (100.0%) Total basepairs processed: 8,100,829 bp Total written (filtered): 8,029,765 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6361 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 9.6% G: 85.6% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 52 463.8 0 52 4 14 115.9 0 14 8 4 0.5 0 4 9 21 0.1 0 1 20 10 384 0.0 1 32 352 11 5815 0.0 1 229 5586 12 50 0.0 1 0 50 13 3 0.0 1 0 3 14 5 0.0 1 0 5 15 1 0.0 1 0 1 151 7 0.0 1 4 3 209 5 0.0 1 4 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.