This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.54 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 82,646 (3.1%) Reads that were too short: 6,924 (0.3%) Reads written (passing filters): 75,722 (2.9%) Total basepairs processed: 791,719,999 bp Total written (filtered): 21,325,500 bp (2.7%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 82646 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 574 642.2 0 574 7 31762 642.2 0 31762 8 34642 642.2 0 34642 9 30 642.2 0 30 10 14 642.2 0 14 11 20 642.2 0 20 12 12 642.2 0 12 13 19 642.2 0 19 14 31 642.2 0 31 15 26 642.2 0 26 16 25 642.2 0 25 17 29 642.2 0 29 18 23 642.2 0 23 19 41 642.2 0 41 20 39 642.2 0 39 21 16 642.2 0 16 22 23 642.2 0 23 23 36 642.2 0 36 24 20 642.2 0 20 25 24 642.2 0 24 26 18 642.2 0 18 27 18 642.2 0 18 28 28 642.2 0 28 29 20 642.2 0 20 30 25 642.2 0 25 31 17 642.2 0 17 32 28 642.2 0 28 33 23 642.2 0 23 34 21 642.2 0 21 35 54 642.2 0 54 36 348 642.2 0 348 37 125 642.2 0 125 38 186 642.2 0 186 39 18 642.2 0 18 40 19 642.2 0 19 41 25 642.2 0 25 42 32 642.2 0 32 43 64 642.2 0 64 44 95 642.2 0 95 45 184 642.2 0 184 46 21 642.2 0 21 47 19 642.2 0 19 48 22 642.2 0 22 49 43 642.2 0 43 50 33 642.2 0 33 51 29 642.2 0 29 52 36 642.2 0 36 53 38 642.2 0 38 54 27 642.2 0 27 55 33 642.2 0 33 56 30 642.2 0 30 57 44 642.2 0 44 58 49 642.2 0 49 59 37 642.2 0 37 60 20 642.2 0 20 61 34 642.2 0 34 62 34 642.2 0 34 63 38 642.2 0 38 64 42 642.2 0 42 65 19 642.2 0 19 66 35 642.2 0 35 67 15 642.2 0 15 68 21 642.2 0 21 69 15 642.2 0 15 70 16 642.2 0 16 71 22 642.2 0 22 72 34 642.2 0 34 73 31 642.2 0 31 74 24 642.2 0 24 75 35 642.2 0 35 76 40 642.2 0 40 77 42 642.2 0 42 78 28 642.2 0 28 79 51 642.2 0 51 80 24 642.2 0 24 81 26 642.2 0 26 82 21 642.2 0 21 83 18 642.2 0 18 84 28 642.2 0 28 85 40 642.2 0 40 86 31 642.2 0 31 87 45 642.2 0 45 88 35 642.2 0 35 89 36 642.2 0 36 90 46 642.2 0 46 91 40 642.2 0 40 92 31 642.2 0 31 93 35 642.2 0 35 94 34 642.2 0 34 95 50 642.2 0 50 96 48 642.2 0 48 97 33 642.2 0 33 98 32 642.2 0 32 99 26 642.2 0 26 100 23 642.2 0 23 101 34 642.2 0 34 102 21 642.2 0 21 103 25 642.2 0 25 104 73 642.2 0 73 105 26 642.2 0 26 106 27 642.2 0 27 107 31 642.2 0 31 108 25 642.2 0 25 109 22 642.2 0 22 110 15 642.2 0 15 111 30 642.2 0 30 112 25 642.2 0 25 113 21 642.2 0 21 114 77 642.2 0 77 115 51 642.2 0 51 116 24 642.2 0 24 117 20 642.2 0 20 118 41 642.2 0 41 119 20 642.2 0 20 120 29 642.2 0 29 121 37 642.2 0 37 122 81 642.2 0 81 123 28 642.2 0 28 124 17 642.2 0 17 125 36 642.2 0 36 126 46 642.2 0 46 127 33 642.2 0 33 128 77 642.2 0 77 129 46 642.2 0 46 130 54 642.2 0 54 131 169 642.2 0 169 132 212 642.2 0 212 133 136 642.2 0 136 134 67 642.2 0 67 135 271 642.2 0 271 136 199 642.2 0 199 137 153 642.2 0 153 138 28 642.2 0 28 139 26 642.2 0 26 140 90 642.2 0 90 141 80 642.2 0 80 142 164 642.2 0 164 143 101 642.2 0 101 144 19 642.2 0 19 145 48 642.2 0 48 146 46 642.2 0 46 147 40 642.2 0 40 148 61 642.2 0 61 149 67 642.2 0 67 150 47 642.2 0 47 151 52 642.2 0 52 152 57 642.2 0 57 153 45 642.2 0 45 154 44 642.2 0 44 155 49 642.2 0 49 156 29 642.2 0 29 157 36 642.2 0 36 158 26 642.2 0 26 159 31 642.2 0 31 160 32 642.2 0 32 161 43 642.2 0 43 162 36 642.2 0 36 163 35 642.2 0 35 164 50 642.2 0 50 165 42 642.2 0 42 166 37 642.2 0 37 167 30 642.2 0 30 168 38 642.2 0 38 169 23 642.2 0 23 170 40 642.2 0 40 171 43 642.2 0 43 172 23 642.2 0 23 173 59 642.2 0 59 174 33 642.2 0 33 175 34 642.2 0 34 176 39 642.2 0 39 177 29 642.2 0 29 178 33 642.2 0 33 179 31 642.2 0 31 180 37 642.2 0 37 181 38 642.2 0 38 182 43 642.2 0 43 183 52 642.2 0 52 184 44 642.2 0 44 185 44 642.2 0 44 186 42 642.2 0 42 187 30 642.2 0 30 188 193 642.2 0 193 189 96 642.2 0 96 190 42 642.2 0 42 191 41 642.2 0 41 192 20 642.2 0 20 193 28 642.2 0 28 194 45 642.2 0 45 195 34 642.2 0 34 196 29 642.2 0 29 197 18 642.2 0 18 198 21 642.2 0 21 199 34 642.2 0 34 200 24 642.2 0 24 201 39 642.2 0 39 202 37 642.2 0 37 203 30 642.2 0 30 204 34 642.2 0 34 205 29 642.2 0 29 206 18 642.2 0 18 207 45 642.2 0 45 208 74 642.2 0 74 209 95 642.2 0 95 210 38 642.2 0 38 211 486 642.2 0 486 212 42 642.2 0 42 213 35 642.2 0 35 214 91 642.2 0 91 215 81 642.2 0 81 216 68 642.2 0 68 217 39 642.2 0 39 218 20 642.2 0 20 219 43 642.2 0 43 220 30 642.2 0 30 221 44 642.2 0 44 222 52 642.2 0 52 223 39 642.2 0 39 224 29 642.2 0 29 225 51 642.2 0 51 226 32 642.2 0 32 227 41 642.2 0 41 228 41 642.2 0 41 229 23 642.2 0 23 230 57 642.2 0 57 231 25 642.2 0 25 232 36 642.2 0 36 233 53 642.2 0 53 234 56 642.2 0 56 235 72 642.2 0 72 236 51 642.2 0 51 237 39 642.2 0 39 238 50 642.2 0 50 239 31 642.2 0 31 240 43 642.2 0 43 241 40 642.2 0 40 242 49 642.2 0 49 243 49 642.2 0 49 244 80 642.2 0 80 245 50 642.2 0 50 246 31 642.2 0 31 247 32 642.2 0 32 248 26 642.2 0 26 249 39 642.2 0 39 250 41 642.2 0 41 251 25 642.2 0 25 252 38 642.2 0 38 253 40 642.2 0 40 254 41 642.2 0 41 255 41 642.2 0 41 256 52 642.2 0 52 257 57 642.2 0 57 258 55 642.2 0 55 259 39 642.2 0 39 260 39 642.2 0 39 261 37 642.2 0 37 262 47 642.2 0 47 263 34 642.2 0 34 264 45 642.2 0 45 265 51 642.2 0 51 266 73 642.2 0 73 267 41 642.2 0 41 268 110 642.2 0 110 269 202 642.2 0 202 270 122 642.2 0 122 271 229 642.2 0 229 272 53 642.2 0 53 273 78 642.2 0 78 274 77 642.2 0 77 275 44 642.2 0 44 276 63 642.2 0 63 277 49 642.2 0 49 278 42 642.2 0 42 279 107 642.2 0 107 280 62 642.2 0 62 281 79 642.2 0 79 282 58 642.2 0 58 283 58 642.2 0 58 284 95 642.2 0 95 285 53 642.2 0 53 286 51 642.2 0 51 287 58 642.2 0 58 288 51 642.2 0 51 289 51 642.2 0 51 290 56 642.2 0 56 291 61 642.2 0 61 292 45 642.2 0 45 293 74 642.2 0 74 294 193 642.2 0 193 295 342 642.2 0 342 296 289 642.2 0 289 297 192 642.2 0 192 298 146 642.2 0 146 299 43 642.2 0 43 300 78 642.2 0 78 301 221 642.2 0 221 Finished in 3.76 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 75,722 Reads with adapters: 31,019 (41.0%) Reads that were too short: 55 (0.1%) Reads written (passing filters): 30,964 (40.9%) Total basepairs processed: 21,325,500 bp Total written (filtered): 8,573,826 bp (40.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31019 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 10 0.0 3 0 0 10 16 197 0.0 3 0 195 2 17 30650 0.0 3 29984 650 15 1 18 58 0.0 3 10 44 3 1 19 14 0.0 3 0 0 11 3 20 14 0.0 3 0 0 0 14 32 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 80 1 0.0 3 1 86 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 217 33 0.0 3 0 0 1 32 221 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 252 1 0.0 3 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 30,964 Reads with adapters: 45 (0.1%) Reads written (passing filters): 30,964 (100.0%) Total basepairs processed: 8,573,826 bp Total written (filtered): 8,573,687 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 45 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 15.6% C: 53.3% G: 24.4% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 43 483.8 0 43 4 1 121.0 0 1 6 1 7.6 0 1