This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.66 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 102,756 (3.9%) Reads that were too short: 18,351 (0.7%) Reads written (passing filters): 84,405 (3.2%) Total basepairs processed: 791,719,999 bp Total written (filtered): 17,824,845 bp (2.3%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 102756 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 915 642.2 0 915 7 10458 642.2 0 10458 8 279 642.2 0 279 9 11795 642.2 0 11795 10 81 642.2 0 81 11 63 642.2 0 63 12 58 642.2 0 58 13 67 642.2 0 67 14 101 642.2 0 101 15 57 642.2 0 57 16 94 642.2 0 94 17 90 642.2 0 90 18 57 642.2 0 57 19 71 642.2 0 71 20 55 642.2 0 55 21 76 642.2 0 76 22 78 642.2 0 78 23 82 642.2 0 82 24 73 642.2 0 73 25 53 642.2 0 53 26 70 642.2 0 70 27 89 642.2 0 89 28 91 642.2 0 91 29 60 642.2 0 60 30 68 642.2 0 68 31 78 642.2 0 78 32 52 642.2 0 52 33 49 642.2 0 49 34 68 642.2 0 68 35 88 642.2 0 88 36 38 642.2 0 38 37 135 642.2 0 135 38 65 642.2 0 65 39 94 642.2 0 94 40 72 642.2 0 72 41 89 642.2 0 89 42 314 642.2 0 314 43 213 642.2 0 213 44 406 642.2 0 406 45 67 642.2 0 67 46 70 642.2 0 70 47 81 642.2 0 81 48 91 642.2 0 91 49 80 642.2 0 80 50 104 642.2 0 104 51 69 642.2 0 69 52 77 642.2 0 77 53 60 642.2 0 60 54 65 642.2 0 65 55 71 642.2 0 71 56 76 642.2 0 76 57 135 642.2 0 135 58 114 642.2 0 114 59 81 642.2 0 81 60 56 642.2 0 56 61 69 642.2 0 69 62 63 642.2 0 63 63 68 642.2 0 68 64 64 642.2 0 64 65 116 642.2 0 116 66 97 642.2 0 97 67 101 642.2 0 101 68 81 642.2 0 81 69 61 642.2 0 61 70 63 642.2 0 63 71 70 642.2 0 70 72 67 642.2 0 67 73 82 642.2 0 82 74 92 642.2 0 92 75 166 642.2 0 166 76 115 642.2 0 115 77 117 642.2 0 117 78 67 642.2 0 67 79 115 642.2 0 115 80 137 642.2 0 137 81 114 642.2 0 114 82 56 642.2 0 56 83 66 642.2 0 66 84 53 642.2 0 53 85 79 642.2 0 79 86 67 642.2 0 67 87 69 642.2 0 69 88 70 642.2 0 70 89 76 642.2 0 76 90 94 642.2 0 94 91 103 642.2 0 103 92 649 642.2 0 649 93 441 642.2 0 441 94 772 642.2 0 772 95 68 642.2 0 68 96 84 642.2 0 84 97 86 642.2 0 86 98 74 642.2 0 74 99 89 642.2 0 89 100 91 642.2 0 91 101 80 642.2 0 80 102 63 642.2 0 63 103 48 642.2 0 48 104 78 642.2 0 78 105 88 642.2 0 88 106 66 642.2 0 66 107 64 642.2 0 64 108 102 642.2 0 102 109 114 642.2 0 114 110 121 642.2 0 121 111 89 642.2 0 89 112 90 642.2 0 90 113 93 642.2 0 93 114 95 642.2 0 95 115 97 642.2 0 97 116 387 642.2 0 387 117 168 642.2 0 168 118 140 642.2 0 140 119 204 642.2 0 204 120 280 642.2 0 280 121 460 642.2 0 460 122 1315 642.2 0 1315 123 33664 642.2 0 33664 124 601 642.2 0 601 125 893 642.2 0 893 126 108 642.2 0 108 127 99 642.2 0 99 128 123 642.2 0 123 129 189 642.2 0 189 130 1005 642.2 0 1005 131 419 642.2 0 419 132 1127 642.2 0 1127 133 72 642.2 0 72 134 58 642.2 0 58 135 50 642.2 0 50 136 78 642.2 0 78 137 74 642.2 0 74 138 96 642.2 0 96 139 97 642.2 0 97 140 68 642.2 0 68 141 73 642.2 0 73 142 57 642.2 0 57 143 78 642.2 0 78 144 60 642.2 0 60 145 61 642.2 0 61 146 88 642.2 0 88 147 70 642.2 0 70 148 31 642.2 0 31 149 38 642.2 0 38 150 56 642.2 0 56 151 49 642.2 0 49 152 56 642.2 0 56 153 83 642.2 0 83 154 68 642.2 0 68 155 56 642.2 0 56 156 47 642.2 0 47 157 42 642.2 0 42 158 65 642.2 0 65 159 62 642.2 0 62 160 63 642.2 0 63 161 61 642.2 0 61 162 69 642.2 0 69 163 65 642.2 0 65 164 71 642.2 0 71 165 71 642.2 0 71 166 58 642.2 0 58 167 121 642.2 0 121 168 115 642.2 0 115 169 94 642.2 0 94 170 45 642.2 0 45 171 67 642.2 0 67 172 61 642.2 0 61 173 147 642.2 0 147 174 77 642.2 0 77 175 288 642.2 0 288 176 197 642.2 0 197 177 188 642.2 0 188 178 370 642.2 0 370 179 235 642.2 0 235 180 708 642.2 0 708 181 184 642.2 0 184 182 90 642.2 0 90 183 73 642.2 0 73 184 216 642.2 0 216 185 81 642.2 0 81 186 86 642.2 0 86 187 54 642.2 0 54 188 85 642.2 0 85 189 68 642.2 0 68 190 65 642.2 0 65 191 69 642.2 0 69 192 95 642.2 0 95 193 131 642.2 0 131 194 121 642.2 0 121 195 174 642.2 0 174 196 234 642.2 0 234 197 144 642.2 0 144 198 386 642.2 0 386 199 535 642.2 0 535 200 500 642.2 0 500 201 589 642.2 0 589 202 349 642.2 0 349 203 135 642.2 0 135 204 103 642.2 0 103 205 189 642.2 0 189 206 176 642.2 0 176 207 181 642.2 0 181 208 151 642.2 0 151 209 79 642.2 0 79 210 85 642.2 0 85 211 89 642.2 0 89 212 68 642.2 0 68 213 81 642.2 0 81 214 81 642.2 0 81 215 112 642.2 0 112 216 118 642.2 0 118 217 82 642.2 0 82 218 125 642.2 0 125 219 294 642.2 0 294 220 387 642.2 0 387 221 515 642.2 0 515 222 920 642.2 0 920 223 359 642.2 0 359 224 199 642.2 0 199 225 596 642.2 0 596 226 87 642.2 0 87 227 94 642.2 0 94 228 122 642.2 0 122 229 125 642.2 0 125 230 144 642.2 0 144 231 75 642.2 0 75 232 83 642.2 0 83 233 80 642.2 0 80 234 73 642.2 0 73 235 86 642.2 0 86 236 76 642.2 0 76 237 95 642.2 0 95 238 237 642.2 0 237 239 321 642.2 0 321 240 1333 642.2 0 1333 241 733 642.2 0 733 242 1089 642.2 0 1089 243 128 642.2 0 128 244 109 642.2 0 109 245 70 642.2 0 70 246 92 642.2 0 92 247 125 642.2 0 125 248 129 642.2 0 129 249 113 642.2 0 113 250 158 642.2 0 158 251 246 642.2 0 246 252 271 642.2 0 271 253 172 642.2 0 172 254 80 642.2 0 80 255 80 642.2 0 80 256 92 642.2 0 92 257 100 642.2 0 100 258 90 642.2 0 90 259 87 642.2 0 87 260 92 642.2 0 92 261 140 642.2 0 140 262 291 642.2 0 291 263 160 642.2 0 160 264 62 642.2 0 62 265 63 642.2 0 63 266 65 642.2 0 65 267 64 642.2 0 64 268 110 642.2 0 110 269 73 642.2 0 73 270 97 642.2 0 97 271 66 642.2 0 66 272 79 642.2 0 79 273 67 642.2 0 67 274 58 642.2 0 58 275 60 642.2 0 60 276 85 642.2 0 85 277 97 642.2 0 97 278 101 642.2 0 101 279 125 642.2 0 125 280 84 642.2 0 84 281 113 642.2 0 113 282 74 642.2 0 74 283 106 642.2 0 106 284 119 642.2 0 119 285 91 642.2 0 91 286 124 642.2 0 124 287 233 642.2 0 233 288 447 642.2 0 447 289 284 642.2 0 284 290 794 642.2 0 794 291 273 642.2 0 273 292 205 642.2 0 205 293 122 642.2 0 122 294 94 642.2 0 94 295 106 642.2 0 106 296 85 642.2 0 85 297 110 642.2 0 110 298 87 642.2 0 87 299 126 642.2 0 126 300 144 642.2 0 144 301 106 642.2 0 106 Finished in 3.78 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 84,405 Reads with adapters: 11,162 (13.2%) Reads that were too short: 84 (0.1%) Reads written (passing filters): 11,078 (13.1%) Total basepairs processed: 17,824,845 bp Total written (filtered): 3,034,223 bp (17.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11162 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 5 0 2 19 163 0.0 3 0 138 15 7 3 20 10840 0.0 4 10120 577 104 31 8 21 20 0.0 4 3 15 1 1 22 7 0.0 4 3 1 1 0 2 23 25 0.0 4 0 0 0 18 7 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 1 52 1 0.0 4 0 0 1 58 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 86 7 0.0 4 0 0 0 0 7 91 2 0.0 4 0 0 0 0 2 93 2 0.0 4 0 0 0 0 2 97 2 0.0 4 0 0 0 0 2 98 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 122 2 0.0 4 1 0 0 0 1 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 1 131 2 0.0 4 0 0 0 0 2 143 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 168 7 0.0 4 0 0 0 0 7 170 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 194 27 0.0 4 0 0 0 0 27 202 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 12 0.0 4 0 0 0 0 12 229 8 0.0 4 0 0 0 1 7 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 11,078 Reads with adapters: 828 (7.5%) Reads written (passing filters): 11,078 (100.0%) Total basepairs processed: 3,034,223 bp Total written (filtered): 3,021,867 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 828 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.5% C: 12.0% G: 74.3% T: 4.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 79 173.1 0 79 4 13 43.3 0 13 9 4 0.0 0 1 3 10 38 0.0 1 3 35 11 481 0.0 1 23 458 12 184 0.0 1 7 177 13 7 0.0 1 0 7 14 4 0.0 1 0 4 210 3 0.0 1 3 224 1 0.0 1 1 225 13 0.0 1 12 1 226 1 0.0 1 1