This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 71.63 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 274,093 (10.4%) Reads that were too short: 60,140 (2.3%) Reads written (passing filters): 213,953 (8.1%) Total basepairs processed: 791,719,999 bp Total written (filtered): 55,700,247 bp (7.0%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 274093 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1509 642.2 0 1509 7 22880 642.2 0 22880 8 1529 642.2 0 1529 9 17430 642.2 0 17430 10 3715 642.2 0 3715 11 101048 642.2 0 101048 12 229 642.2 0 229 13 109 642.2 0 109 14 117 642.2 0 117 15 117 642.2 0 117 16 88 642.2 0 88 17 97 642.2 0 97 18 112 642.2 0 112 19 108 642.2 0 108 20 105 642.2 0 105 21 71 642.2 0 71 22 103 642.2 0 103 23 118 642.2 0 118 24 120 642.2 0 120 25 84 642.2 0 84 26 151 642.2 0 151 27 210 642.2 0 210 28 200 642.2 0 200 29 619 642.2 0 619 30 111 642.2 0 111 31 111 642.2 0 111 32 142 642.2 0 142 33 125 642.2 0 125 34 129 642.2 0 129 35 101 642.2 0 101 36 102 642.2 0 102 37 128 642.2 0 128 38 110 642.2 0 110 39 116 642.2 0 116 40 334 642.2 0 334 41 281 642.2 0 281 42 119 642.2 0 119 43 122 642.2 0 122 44 123 642.2 0 123 45 244 642.2 0 244 46 100 642.2 0 100 47 132 642.2 0 132 48 224 642.2 0 224 49 443 642.2 0 443 50 107 642.2 0 107 51 124 642.2 0 124 52 109 642.2 0 109 53 136 642.2 0 136 54 130 642.2 0 130 55 182 642.2 0 182 56 161 642.2 0 161 57 147 642.2 0 147 58 137 642.2 0 137 59 259 642.2 0 259 60 172 642.2 0 172 61 211 642.2 0 211 62 159 642.2 0 159 63 96 642.2 0 96 64 117 642.2 0 117 65 112 642.2 0 112 66 99 642.2 0 99 67 294 642.2 0 294 68 146 642.2 0 146 69 180 642.2 0 180 70 185 642.2 0 185 71 751 642.2 0 751 72 135 642.2 0 135 73 116 642.2 0 116 74 93 642.2 0 93 75 111 642.2 0 111 76 122 642.2 0 122 77 109 642.2 0 109 78 227 642.2 0 227 79 683 642.2 0 683 80 718 642.2 0 718 81 316 642.2 0 316 82 593 642.2 0 593 83 472 642.2 0 472 84 765 642.2 0 765 85 699 642.2 0 699 86 119 642.2 0 119 87 76 642.2 0 76 88 88 642.2 0 88 89 162 642.2 0 162 90 162 642.2 0 162 91 148 642.2 0 148 92 89 642.2 0 89 93 115 642.2 0 115 94 104 642.2 0 104 95 130 642.2 0 130 96 136 642.2 0 136 97 222 642.2 0 222 98 1864 642.2 0 1864 99 231 642.2 0 231 100 221 642.2 0 221 101 488 642.2 0 488 102 122 642.2 0 122 103 105 642.2 0 105 104 148 642.2 0 148 105 153 642.2 0 153 106 161 642.2 0 161 107 316 642.2 0 316 108 183 642.2 0 183 109 301 642.2 0 301 110 314 642.2 0 314 111 376 642.2 0 376 112 1528 642.2 0 1528 113 7272 642.2 0 7272 114 3398 642.2 0 3398 115 15310 642.2 0 15310 116 164 642.2 0 164 117 126 642.2 0 126 118 101 642.2 0 101 119 195 642.2 0 195 120 113 642.2 0 113 121 106 642.2 0 106 122 119 642.2 0 119 123 135 642.2 0 135 124 234 642.2 0 234 125 185 642.2 0 185 126 166 642.2 0 166 127 137 642.2 0 137 128 286 642.2 0 286 129 280 642.2 0 280 130 583 642.2 0 583 131 460 642.2 0 460 132 107 642.2 0 107 133 118 642.2 0 118 134 732 642.2 0 732 135 129 642.2 0 129 136 225 642.2 0 225 137 144 642.2 0 144 138 237 642.2 0 237 139 280 642.2 0 280 140 182 642.2 0 182 141 318 642.2 0 318 142 322 642.2 0 322 143 200 642.2 0 200 144 485 642.2 0 485 145 157 642.2 0 157 146 118 642.2 0 118 147 135 642.2 0 135 148 105 642.2 0 105 149 139 642.2 0 139 150 145 642.2 0 145 151 93 642.2 0 93 152 94 642.2 0 94 153 138 642.2 0 138 154 363 642.2 0 363 155 656 642.2 0 656 156 1089 642.2 0 1089 157 441 642.2 0 441 158 135 642.2 0 135 159 99 642.2 0 99 160 113 642.2 0 113 161 105 642.2 0 105 162 98 642.2 0 98 163 132 642.2 0 132 164 199 642.2 0 199 165 108 642.2 0 108 166 137 642.2 0 137 167 390 642.2 0 390 168 295 642.2 0 295 169 127 642.2 0 127 170 84 642.2 0 84 171 236 642.2 0 236 172 175 642.2 0 175 173 80 642.2 0 80 174 93 642.2 0 93 175 109 642.2 0 109 176 87 642.2 0 87 177 106 642.2 0 106 178 203 642.2 0 203 179 87 642.2 0 87 180 135 642.2 0 135 181 60 642.2 0 60 182 79 642.2 0 79 183 85 642.2 0 85 184 149 642.2 0 149 185 70 642.2 0 70 186 120 642.2 0 120 187 117 642.2 0 117 188 105 642.2 0 105 189 248 642.2 0 248 190 124 642.2 0 124 191 102 642.2 0 102 192 106 642.2 0 106 193 116 642.2 0 116 194 111 642.2 0 111 195 103 642.2 0 103 196 114 642.2 0 114 197 178 642.2 0 178 198 89 642.2 0 89 199 103 642.2 0 103 200 204 642.2 0 204 201 314 642.2 0 314 202 408 642.2 0 408 203 129 642.2 0 129 204 120 642.2 0 120 205 99 642.2 0 99 206 192 642.2 0 192 207 89 642.2 0 89 208 134 642.2 0 134 209 134 642.2 0 134 210 178 642.2 0 178 211 351 642.2 0 351 212 420 642.2 0 420 213 574 642.2 0 574 214 139 642.2 0 139 215 134 642.2 0 134 216 160 642.2 0 160 217 112 642.2 0 112 218 92 642.2 0 92 219 72 642.2 0 72 220 102 642.2 0 102 221 130 642.2 0 130 222 120 642.2 0 120 223 113 642.2 0 113 224 318 642.2 0 318 225 122 642.2 0 122 226 163 642.2 0 163 227 162 642.2 0 162 228 784 642.2 0 784 229 516 642.2 0 516 230 1014 642.2 0 1014 231 272 642.2 0 272 232 400 642.2 0 400 233 147 642.2 0 147 234 240 642.2 0 240 235 351 642.2 0 351 236 265 642.2 0 265 237 289 642.2 0 289 238 187 642.2 0 187 239 110 642.2 0 110 240 123 642.2 0 123 241 92 642.2 0 92 242 135 642.2 0 135 243 130 642.2 0 130 244 88 642.2 0 88 245 110 642.2 0 110 246 173 642.2 0 173 247 266 642.2 0 266 248 231 642.2 0 231 249 257 642.2 0 257 250 143 642.2 0 143 251 109 642.2 0 109 252 123 642.2 0 123 253 163 642.2 0 163 254 189 642.2 0 189 255 249 642.2 0 249 256 422 642.2 0 422 257 291 642.2 0 291 258 279 642.2 0 279 259 198 642.2 0 198 260 89 642.2 0 89 261 122 642.2 0 122 262 87 642.2 0 87 263 114 642.2 0 114 264 104 642.2 0 104 265 109 642.2 0 109 266 110 642.2 0 110 267 82 642.2 0 82 268 251 642.2 0 251 269 439 642.2 0 439 270 671 642.2 0 671 271 292 642.2 0 292 272 145 642.2 0 145 273 164 642.2 0 164 274 211 642.2 0 211 275 185 642.2 0 185 276 125 642.2 0 125 277 137 642.2 0 137 278 148 642.2 0 148 279 147 642.2 0 147 280 401 642.2 0 401 281 224 642.2 0 224 282 184 642.2 0 184 283 204 642.2 0 204 284 153 642.2 0 153 285 184 642.2 0 184 286 164 642.2 0 164 287 156 642.2 0 156 288 242 642.2 0 242 289 181 642.2 0 181 290 228 642.2 0 228 291 243 642.2 0 243 292 239 642.2 0 239 293 322 642.2 0 322 294 789 642.2 0 789 295 1826 642.2 0 1826 296 4232 642.2 0 4232 297 3415 642.2 0 3415 298 19628 642.2 0 19628 299 3426 642.2 0 3426 300 6550 642.2 0 6550 301 304 642.2 0 304 Finished in 10.43 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 213,953 Reads with adapters: 17,899 (8.4%) Reads that were too short: 69 (0.0%) Reads written (passing filters): 17,830 (8.3%) Total basepairs processed: 55,700,247 bp Total written (filtered): 4,824,684 bp (8.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17899 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 2 3 18 19 0.0 3 0 0 9 5 5 19 267 0.0 3 0 194 31 21 21 20 17130 0.0 4 15613 1166 261 72 18 21 53 0.0 4 14 27 7 3 2 22 5 0.0 4 0 0 2 1 2 23 97 0.0 4 0 0 0 67 30 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 1 38 1 0.0 4 1 44 2 0.0 4 0 0 0 1 1 59 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 1 71 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 1 87 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 1 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 1 100 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 1 109 1 0.0 4 0 0 0 0 1 116 1 0.0 4 0 0 0 0 1 121 25 0.0 4 0 0 0 0 25 123 1 0.0 4 0 1 126 2 0.0 4 0 0 0 0 2 127 217 0.0 4 0 0 0 0 217 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 0 1 151 7 0.0 4 0 0 0 0 7 152 3 0.0 4 0 0 0 0 3 153 3 0.0 4 0 0 0 0 3 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 1 169 3 0.0 4 0 0 0 0 3 170 1 0.0 4 0 0 0 0 1 171 6 0.0 4 0 0 0 0 6 174 1 0.0 4 0 0 0 1 176 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 1 198 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 214 3 0.0 4 0 0 0 0 3 215 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 235 2 0.0 4 0 0 0 0 2 268 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 3 0.0 4 0 0 0 0 3 290 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.66 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 17,830 Reads with adapters: 4,297 (24.1%) Reads written (passing filters): 17,830 (100.0%) Total basepairs processed: 4,824,684 bp Total written (filtered): 4,779,617 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4297 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 9.9% G: 85.2% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 11 278.6 0 11 4 10 69.6 0 10 6 3 4.4 0 3 7 2 1.1 0 2 8 6 0.3 0 6 9 716 0.1 0 93 623 10 3070 0.0 1 232 2838 11 438 0.0 1 11 427 12 13 0.0 1 1 12 13 17 0.0 1 0 17 153 1 0.0 1 1 177 1 0.0 1 1 208 2 0.0 1 2 211 1 0.0 1 0 1 268 1 0.0 1 1 269 2 0.0 1 1 1 270 2 0.0 1 1 1 272 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.