This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 72.43 s (28 us/read; 2.18 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 295,805 (11.2%) Reads that were too short: 60,315 (2.3%) Reads written (passing filters): 235,490 (9.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 61,766,190 bp (7.8%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 295805 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1644 642.2 0 1644 7 19889 642.2 0 19889 8 1394 642.2 0 1394 9 22237 642.2 0 22237 10 3102 642.2 0 3102 11 120457 642.2 0 120457 12 261 642.2 0 261 13 99 642.2 0 99 14 116 642.2 0 116 15 114 642.2 0 114 16 91 642.2 0 91 17 74 642.2 0 74 18 78 642.2 0 78 19 82 642.2 0 82 20 91 642.2 0 91 21 90 642.2 0 90 22 79 642.2 0 79 23 105 642.2 0 105 24 93 642.2 0 93 25 84 642.2 0 84 26 107 642.2 0 107 27 114 642.2 0 114 28 98 642.2 0 98 29 655 642.2 0 655 30 110 642.2 0 110 31 79 642.2 0 79 32 119 642.2 0 119 33 107 642.2 0 107 34 113 642.2 0 113 35 112 642.2 0 112 36 134 642.2 0 134 37 111 642.2 0 111 38 110 642.2 0 110 39 131 642.2 0 131 40 494 642.2 0 494 41 342 642.2 0 342 42 104 642.2 0 104 43 96 642.2 0 96 44 137 642.2 0 137 45 322 642.2 0 322 46 93 642.2 0 93 47 116 642.2 0 116 48 246 642.2 0 246 49 463 642.2 0 463 50 128 642.2 0 128 51 101 642.2 0 101 52 115 642.2 0 115 53 112 642.2 0 112 54 105 642.2 0 105 55 181 642.2 0 181 56 132 642.2 0 132 57 123 642.2 0 123 58 125 642.2 0 125 59 298 642.2 0 298 60 176 642.2 0 176 61 231 642.2 0 231 62 177 642.2 0 177 63 128 642.2 0 128 64 147 642.2 0 147 65 147 642.2 0 147 66 101 642.2 0 101 67 301 642.2 0 301 68 137 642.2 0 137 69 159 642.2 0 159 70 162 642.2 0 162 71 713 642.2 0 713 72 155 642.2 0 155 73 123 642.2 0 123 74 106 642.2 0 106 75 101 642.2 0 101 76 111 642.2 0 111 77 121 642.2 0 121 78 144 642.2 0 144 79 334 642.2 0 334 80 496 642.2 0 496 81 235 642.2 0 235 82 683 642.2 0 683 83 566 642.2 0 566 84 824 642.2 0 824 85 684 642.2 0 684 86 95 642.2 0 95 87 101 642.2 0 101 88 101 642.2 0 101 89 163 642.2 0 163 90 149 642.2 0 149 91 156 642.2 0 156 92 98 642.2 0 98 93 125 642.2 0 125 94 105 642.2 0 105 95 142 642.2 0 142 96 124 642.2 0 124 97 196 642.2 0 196 98 1823 642.2 0 1823 99 248 642.2 0 248 100 280 642.2 0 280 101 553 642.2 0 553 102 119 642.2 0 119 103 120 642.2 0 120 104 159 642.2 0 159 105 184 642.2 0 184 106 191 642.2 0 191 107 337 642.2 0 337 108 193 642.2 0 193 109 287 642.2 0 287 110 384 642.2 0 384 111 383 642.2 0 383 112 1493 642.2 0 1493 113 7062 642.2 0 7062 114 2903 642.2 0 2903 115 16657 642.2 0 16657 116 156 642.2 0 156 117 134 642.2 0 134 118 88 642.2 0 88 119 164 642.2 0 164 120 134 642.2 0 134 121 128 642.2 0 128 122 113 642.2 0 113 123 143 642.2 0 143 124 280 642.2 0 280 125 181 642.2 0 181 126 138 642.2 0 138 127 138 642.2 0 138 128 268 642.2 0 268 129 226 642.2 0 226 130 547 642.2 0 547 131 469 642.2 0 469 132 126 642.2 0 126 133 131 642.2 0 131 134 584 642.2 0 584 135 117 642.2 0 117 136 134 642.2 0 134 137 108 642.2 0 108 138 201 642.2 0 201 139 134 642.2 0 134 140 158 642.2 0 158 141 154 642.2 0 154 142 272 642.2 0 272 143 203 642.2 0 203 144 450 642.2 0 450 145 144 642.2 0 144 146 131 642.2 0 131 147 153 642.2 0 153 148 96 642.2 0 96 149 135 642.2 0 135 150 141 642.2 0 141 151 108 642.2 0 108 152 121 642.2 0 121 153 146 642.2 0 146 154 433 642.2 0 433 155 872 642.2 0 872 156 1465 642.2 0 1465 157 633 642.2 0 633 158 187 642.2 0 187 159 90 642.2 0 90 160 86 642.2 0 86 161 126 642.2 0 126 162 97 642.2 0 97 163 132 642.2 0 132 164 223 642.2 0 223 165 118 642.2 0 118 166 143 642.2 0 143 167 414 642.2 0 414 168 241 642.2 0 241 169 116 642.2 0 116 170 91 642.2 0 91 171 282 642.2 0 282 172 166 642.2 0 166 173 100 642.2 0 100 174 110 642.2 0 110 175 116 642.2 0 116 176 96 642.2 0 96 177 107 642.2 0 107 178 230 642.2 0 230 179 131 642.2 0 131 180 144 642.2 0 144 181 86 642.2 0 86 182 105 642.2 0 105 183 112 642.2 0 112 184 129 642.2 0 129 185 96 642.2 0 96 186 126 642.2 0 126 187 127 642.2 0 127 188 117 642.2 0 117 189 345 642.2 0 345 190 118 642.2 0 118 191 125 642.2 0 125 192 94 642.2 0 94 193 133 642.2 0 133 194 99 642.2 0 99 195 86 642.2 0 86 196 137 642.2 0 137 197 178 642.2 0 178 198 107 642.2 0 107 199 108 642.2 0 108 200 163 642.2 0 163 201 390 642.2 0 390 202 564 642.2 0 564 203 91 642.2 0 91 204 120 642.2 0 120 205 125 642.2 0 125 206 215 642.2 0 215 207 127 642.2 0 127 208 170 642.2 0 170 209 107 642.2 0 107 210 144 642.2 0 144 211 438 642.2 0 438 212 152 642.2 0 152 213 201 642.2 0 201 214 105 642.2 0 105 215 95 642.2 0 95 216 115 642.2 0 115 217 96 642.2 0 96 218 123 642.2 0 123 219 112 642.2 0 112 220 91 642.2 0 91 221 111 642.2 0 111 222 131 642.2 0 131 223 125 642.2 0 125 224 359 642.2 0 359 225 127 642.2 0 127 226 135 642.2 0 135 227 143 642.2 0 143 228 262 642.2 0 262 229 183 642.2 0 183 230 372 642.2 0 372 231 133 642.2 0 133 232 148 642.2 0 148 233 112 642.2 0 112 234 130 642.2 0 130 235 169 642.2 0 169 236 138 642.2 0 138 237 147 642.2 0 147 238 95 642.2 0 95 239 151 642.2 0 151 240 124 642.2 0 124 241 102 642.2 0 102 242 144 642.2 0 144 243 131 642.2 0 131 244 87 642.2 0 87 245 103 642.2 0 103 246 94 642.2 0 94 247 136 642.2 0 136 248 142 642.2 0 142 249 215 642.2 0 215 250 116 642.2 0 116 251 91 642.2 0 91 252 101 642.2 0 101 253 103 642.2 0 103 254 124 642.2 0 124 255 135 642.2 0 135 256 175 642.2 0 175 257 148 642.2 0 148 258 166 642.2 0 166 259 235 642.2 0 235 260 104 642.2 0 104 261 111 642.2 0 111 262 93 642.2 0 93 263 112 642.2 0 112 264 118 642.2 0 118 265 115 642.2 0 115 266 148 642.2 0 148 267 98 642.2 0 98 268 178 642.2 0 178 269 219 642.2 0 219 270 294 642.2 0 294 271 159 642.2 0 159 272 102 642.2 0 102 273 147 642.2 0 147 274 154 642.2 0 154 275 124 642.2 0 124 276 135 642.2 0 135 277 110 642.2 0 110 278 140 642.2 0 140 279 110 642.2 0 110 280 349 642.2 0 349 281 170 642.2 0 170 282 152 642.2 0 152 283 146 642.2 0 146 284 111 642.2 0 111 285 202 642.2 0 202 286 167 642.2 0 167 287 133 642.2 0 133 288 238 642.2 0 238 289 162 642.2 0 162 290 189 642.2 0 189 291 243 642.2 0 243 292 285 642.2 0 285 293 270 642.2 0 270 294 531 642.2 0 531 295 1004 642.2 0 1004 296 2055 642.2 0 2055 297 1556 642.2 0 1556 298 25441 642.2 0 25441 299 4745 642.2 0 4745 300 10176 642.2 0 10176 301 185 642.2 0 185 Finished in 10.20 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 235,490 Reads with adapters: 19,047 (8.1%) Reads that were too short: 113 (0.0%) Reads written (passing filters): 18,934 (8.0%) Total basepairs processed: 61,766,190 bp Total written (filtered): 5,217,676 bp (8.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 19047 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 110 0.0 3 0 106 3 1 17 18508 0.0 3 18101 389 16 2 18 45 0.0 3 7 36 2 19 14 0.0 3 0 0 12 2 20 14 0.0 3 1 0 0 13 23 7 0.0 3 0 0 0 7 25 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 32 15 0.0 3 0 0 0 15 35 7 0.0 3 0 0 0 7 37 1 0.0 3 0 0 1 38 2 0.0 3 0 0 0 2 40 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 50 13 0.0 3 0 0 0 13 53 10 0.0 3 0 0 0 10 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 1 68 1 0.0 3 1 70 1 0.0 3 0 0 0 1 73 124 0.0 3 0 0 110 14 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 6 0.0 3 0 0 0 6 77 6 0.0 3 0 0 0 6 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 1 85 1 0.0 3 1 86 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 2 0.0 3 1 0 0 1 94 7 0.0 3 0 0 0 7 97 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 1 112 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 1 119 1 0.0 3 0 0 1 120 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 125 12 0.0 3 0 0 0 12 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 1 1 146 2 0.0 3 1 0 0 1 147 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 175 3 0.0 3 1 0 0 2 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 192 1 0.0 3 0 0 1 200 26 0.0 3 0 0 0 26 205 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 236 24 0.0 3 0 0 0 24 238 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 285 1 0.0 3 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.67 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 18,934 Reads with adapters: 27 (0.1%) Reads written (passing filters): 18,934 (100.0%) Total basepairs processed: 5,217,676 bp Total written (filtered): 5,217,581 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 27 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.6% C: 25.9% G: 33.3% T: 11.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 20 295.8 0 20 4 3 74.0 0 3 5 1 18.5 0 1 6 3 4.6 0 3