This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.01 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 71,272 (2.7%) Reads that were too short: 17,808 (0.7%) Reads written (passing filters): 53,464 (2.0%) Total basepairs processed: 791,719,999 bp Total written (filtered): 11,753,153 bp (1.5%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 71272 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 296 642.2 0 296 7 4723 642.2 0 4723 8 454 642.2 0 454 9 4190 642.2 0 4190 10 207 642.2 0 207 11 191 642.2 0 191 12 138 642.2 0 138 13 215 642.2 0 215 14 187 642.2 0 187 15 206 642.2 0 206 16 219 642.2 0 219 17 200 642.2 0 200 18 180 642.2 0 180 19 202 642.2 0 202 20 221 642.2 0 221 21 226 642.2 0 226 22 266 642.2 0 266 23 181 642.2 0 181 24 191 642.2 0 191 25 248 642.2 0 248 26 258 642.2 0 258 27 211 642.2 0 211 28 214 642.2 0 214 29 207 642.2 0 207 30 224 642.2 0 224 31 217 642.2 0 217 32 195 642.2 0 195 33 287 642.2 0 287 34 580 642.2 0 580 35 1999 642.2 0 1999 36 1274 642.2 0 1274 37 973 642.2 0 973 38 169 642.2 0 169 39 180 642.2 0 180 40 247 642.2 0 247 41 195 642.2 0 195 42 194 642.2 0 194 43 193 642.2 0 193 44 212 642.2 0 212 45 174 642.2 0 174 46 200 642.2 0 200 47 164 642.2 0 164 48 155 642.2 0 155 49 182 642.2 0 182 50 181 642.2 0 181 51 161 642.2 0 161 52 209 642.2 0 209 53 183 642.2 0 183 54 159 642.2 0 159 55 214 642.2 0 214 56 198 642.2 0 198 57 172 642.2 0 172 58 208 642.2 0 208 59 246 642.2 0 246 60 336 642.2 0 336 61 171 642.2 0 171 62 329 642.2 0 329 63 172 642.2 0 172 64 183 642.2 0 183 65 163 642.2 0 163 66 153 642.2 0 153 67 149 642.2 0 149 68 220 642.2 0 220 69 250 642.2 0 250 70 329 642.2 0 329 71 205 642.2 0 205 72 184 642.2 0 184 73 182 642.2 0 182 74 203 642.2 0 203 75 154 642.2 0 154 76 168 642.2 0 168 77 172 642.2 0 172 78 242 642.2 0 242 79 221 642.2 0 221 80 213 642.2 0 213 81 175 642.2 0 175 82 217 642.2 0 217 83 178 642.2 0 178 84 178 642.2 0 178 85 177 642.2 0 177 86 166 642.2 0 166 87 169 642.2 0 169 88 166 642.2 0 166 89 162 642.2 0 162 90 217 642.2 0 217 91 211 642.2 0 211 92 199 642.2 0 199 93 158 642.2 0 158 94 214 642.2 0 214 95 198 642.2 0 198 96 235 642.2 0 235 97 203 642.2 0 203 98 207 642.2 0 207 99 226 642.2 0 226 100 272 642.2 0 272 101 449 642.2 0 449 102 223 642.2 0 223 103 222 642.2 0 222 104 219 642.2 0 219 105 221 642.2 0 221 106 208 642.2 0 208 107 200 642.2 0 200 108 171 642.2 0 171 109 198 642.2 0 198 110 323 642.2 0 323 111 835 642.2 0 835 112 775 642.2 0 775 113 251 642.2 0 251 114 212 642.2 0 212 115 198 642.2 0 198 116 210 642.2 0 210 117 192 642.2 0 192 118 210 642.2 0 210 119 227 642.2 0 227 120 184 642.2 0 184 121 296 642.2 0 296 122 235 642.2 0 235 123 190 642.2 0 190 124 238 642.2 0 238 125 170 642.2 0 170 126 178 642.2 0 178 127 232 642.2 0 232 128 184 642.2 0 184 129 172 642.2 0 172 130 154 642.2 0 154 131 283 642.2 0 283 132 219 642.2 0 219 133 278 642.2 0 278 134 191 642.2 0 191 135 182 642.2 0 182 136 173 642.2 0 173 137 167 642.2 0 167 138 200 642.2 0 200 139 320 642.2 0 320 140 325 642.2 0 325 141 332 642.2 0 332 142 171 642.2 0 171 143 176 642.2 0 176 144 164 642.2 0 164 145 164 642.2 0 164 146 202 642.2 0 202 147 370 642.2 0 370 148 249 642.2 0 249 149 309 642.2 0 309 150 157 642.2 0 157 151 138 642.2 0 138 152 198 642.2 0 198 153 191 642.2 0 191 154 141 642.2 0 141 155 148 642.2 0 148 156 173 642.2 0 173 157 185 642.2 0 185 158 170 642.2 0 170 159 239 642.2 0 239 160 166 642.2 0 166 161 264 642.2 0 264 162 132 642.2 0 132 163 123 642.2 0 123 164 138 642.2 0 138 165 169 642.2 0 169 166 153 642.2 0 153 167 175 642.2 0 175 168 183 642.2 0 183 169 294 642.2 0 294 170 307 642.2 0 307 171 164 642.2 0 164 172 185 642.2 0 185 173 178 642.2 0 178 174 159 642.2 0 159 175 202 642.2 0 202 176 120 642.2 0 120 177 173 642.2 0 173 178 142 642.2 0 142 179 181 642.2 0 181 180 161 642.2 0 161 181 166 642.2 0 166 182 190 642.2 0 190 183 169 642.2 0 169 184 156 642.2 0 156 185 196 642.2 0 196 186 191 642.2 0 191 187 202 642.2 0 202 188 156 642.2 0 156 189 164 642.2 0 164 190 177 642.2 0 177 191 162 642.2 0 162 192 181 642.2 0 181 193 151 642.2 0 151 194 180 642.2 0 180 195 156 642.2 0 156 196 156 642.2 0 156 197 138 642.2 0 138 198 160 642.2 0 160 199 157 642.2 0 157 200 180 642.2 0 180 201 212 642.2 0 212 202 185 642.2 0 185 203 173 642.2 0 173 204 177 642.2 0 177 205 220 642.2 0 220 206 227 642.2 0 227 207 191 642.2 0 191 208 245 642.2 0 245 209 212 642.2 0 212 210 169 642.2 0 169 211 205 642.2 0 205 212 150 642.2 0 150 213 162 642.2 0 162 214 174 642.2 0 174 215 138 642.2 0 138 216 164 642.2 0 164 217 144 642.2 0 144 218 151 642.2 0 151 219 149 642.2 0 149 220 184 642.2 0 184 221 197 642.2 0 197 222 245 642.2 0 245 223 165 642.2 0 165 224 160 642.2 0 160 225 150 642.2 0 150 226 130 642.2 0 130 227 142 642.2 0 142 228 159 642.2 0 159 229 168 642.2 0 168 230 164 642.2 0 164 231 192 642.2 0 192 232 241 642.2 0 241 233 210 642.2 0 210 234 248 642.2 0 248 235 1197 642.2 0 1197 236 196 642.2 0 196 237 510 642.2 0 510 238 137 642.2 0 137 239 134 642.2 0 134 240 169 642.2 0 169 241 248 642.2 0 248 242 213 642.2 0 213 243 208 642.2 0 208 244 145 642.2 0 145 245 98 642.2 0 98 246 133 642.2 0 133 247 120 642.2 0 120 248 297 642.2 0 297 249 157 642.2 0 157 250 178 642.2 0 178 251 123 642.2 0 123 252 146 642.2 0 146 253 129 642.2 0 129 254 165 642.2 0 165 255 237 642.2 0 237 256 253 642.2 0 253 257 205 642.2 0 205 258 262 642.2 0 262 259 251 642.2 0 251 260 319 642.2 0 319 261 301 642.2 0 301 262 226 642.2 0 226 263 129 642.2 0 129 264 125 642.2 0 125 265 116 642.2 0 116 266 107 642.2 0 107 267 120 642.2 0 120 268 115 642.2 0 115 269 101 642.2 0 101 270 100 642.2 0 100 271 120 642.2 0 120 272 111 642.2 0 111 273 133 642.2 0 133 274 132 642.2 0 132 275 159 642.2 0 159 276 127 642.2 0 127 277 118 642.2 0 118 278 138 642.2 0 138 279 163 642.2 0 163 280 146 642.2 0 146 281 137 642.2 0 137 282 161 642.2 0 161 283 134 642.2 0 134 284 150 642.2 0 150 285 160 642.2 0 160 286 104 642.2 0 104 287 115 642.2 0 115 288 119 642.2 0 119 289 131 642.2 0 131 290 113 642.2 0 113 291 105 642.2 0 105 292 101 642.2 0 101 293 145 642.2 0 145 294 150 642.2 0 150 295 147 642.2 0 147 296 143 642.2 0 143 297 134 642.2 0 134 298 120 642.2 0 120 299 129 642.2 0 129 300 152 642.2 0 152 301 150 642.2 0 150 Finished in 2.44 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 53,464 Reads with adapters: 4,713 (8.8%) Reads that were too short: 32 (0.1%) Reads written (passing filters): 4,681 (8.8%) Total basepairs processed: 11,753,153 bp Total written (filtered): 1,264,302 bp (10.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4713 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 3 1 19 65 0.0 3 0 55 5 1 4 20 4413 0.0 4 4116 234 43 15 5 21 25 0.0 4 9 12 2 0 2 23 5 0.0 4 1 0 0 2 2 30 1 0.0 4 0 1 50 2 0.0 4 0 0 0 0 2 64 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 99 33 0.0 4 0 0 0 0 33 102 3 0.0 4 0 0 0 0 3 104 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 125 0.0 4 0 0 0 0 125 132 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 1 0 0 1 144 1 0.0 4 0 0 1 152 2 0.0 4 0 0 0 0 2 180 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 200 2 0.0 4 0 0 0 0 2 205 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 2 7 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 1 237 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 4,681 Reads with adapters: 265 (5.7%) Reads written (passing filters): 4,681 (100.0%) Total basepairs processed: 1,264,302 bp Total written (filtered): 1,259,519 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 265 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.8% C: 12.8% G: 74.0% T: 3.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 22 73.1 0 22 4 6 18.3 0 6 9 1 0.0 0 0 1 10 1 0.0 1 0 1 11 113 0.0 1 5 108 12 105 0.0 1 4 101 13 1 0.0 1 0 1 15 2 0.0 1 0 2 16 1 0.0 1 0 1 24 3 0.0 1 2 1 152 1 0.0 1 0 1 153 1 0.0 1 1 179 1 0.0 1 0 1 210 4 0.0 1 3 1 225 1 0.0 1 1 226 1 0.0 1 0 1 265 1 0.0 1 1