This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.87 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 61,250 (2.3%) Reads that were too short: 15,814 (0.6%) Reads written (passing filters): 45,436 (1.7%) Total basepairs processed: 791,719,999 bp Total written (filtered): 10,056,424 bp (1.3%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 61250 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 238 642.2 0 238 7 5344 642.2 0 5344 8 370 642.2 0 370 9 4115 642.2 0 4115 10 201 642.2 0 201 11 208 642.2 0 208 12 176 642.2 0 176 13 190 642.2 0 190 14 174 642.2 0 174 15 191 642.2 0 191 16 202 642.2 0 202 17 179 642.2 0 179 18 181 642.2 0 181 19 174 642.2 0 174 20 171 642.2 0 171 21 197 642.2 0 197 22 192 642.2 0 192 23 166 642.2 0 166 24 172 642.2 0 172 25 198 642.2 0 198 26 197 642.2 0 197 27 171 642.2 0 171 28 165 642.2 0 165 29 178 642.2 0 178 30 168 642.2 0 168 31 160 642.2 0 160 32 185 642.2 0 185 33 164 642.2 0 164 34 358 642.2 0 358 35 606 642.2 0 606 36 783 642.2 0 783 37 851 642.2 0 851 38 147 642.2 0 147 39 156 642.2 0 156 40 223 642.2 0 223 41 152 642.2 0 152 42 130 642.2 0 130 43 148 642.2 0 148 44 131 642.2 0 131 45 149 642.2 0 149 46 161 642.2 0 161 47 137 642.2 0 137 48 145 642.2 0 145 49 147 642.2 0 147 50 168 642.2 0 168 51 158 642.2 0 158 52 201 642.2 0 201 53 147 642.2 0 147 54 181 642.2 0 181 55 142 642.2 0 142 56 166 642.2 0 166 57 141 642.2 0 141 58 169 642.2 0 169 59 230 642.2 0 230 60 285 642.2 0 285 61 172 642.2 0 172 62 269 642.2 0 269 63 187 642.2 0 187 64 190 642.2 0 190 65 169 642.2 0 169 66 159 642.2 0 159 67 173 642.2 0 173 68 148 642.2 0 148 69 182 642.2 0 182 70 275 642.2 0 275 71 167 642.2 0 167 72 177 642.2 0 177 73 153 642.2 0 153 74 186 642.2 0 186 75 153 642.2 0 153 76 184 642.2 0 184 77 143 642.2 0 143 78 179 642.2 0 179 79 179 642.2 0 179 80 175 642.2 0 175 81 145 642.2 0 145 82 144 642.2 0 144 83 141 642.2 0 141 84 194 642.2 0 194 85 156 642.2 0 156 86 144 642.2 0 144 87 151 642.2 0 151 88 164 642.2 0 164 89 165 642.2 0 165 90 208 642.2 0 208 91 157 642.2 0 157 92 167 642.2 0 167 93 141 642.2 0 141 94 166 642.2 0 166 95 163 642.2 0 163 96 199 642.2 0 199 97 164 642.2 0 164 98 189 642.2 0 189 99 230 642.2 0 230 100 189 642.2 0 189 101 272 642.2 0 272 102 140 642.2 0 140 103 199 642.2 0 199 104 199 642.2 0 199 105 201 642.2 0 201 106 178 642.2 0 178 107 190 642.2 0 190 108 171 642.2 0 171 109 181 642.2 0 181 110 238 642.2 0 238 111 623 642.2 0 623 112 399 642.2 0 399 113 212 642.2 0 212 114 170 642.2 0 170 115 142 642.2 0 142 116 160 642.2 0 160 117 158 642.2 0 158 118 177 642.2 0 177 119 195 642.2 0 195 120 124 642.2 0 124 121 251 642.2 0 251 122 184 642.2 0 184 123 158 642.2 0 158 124 156 642.2 0 156 125 153 642.2 0 153 126 141 642.2 0 141 127 164 642.2 0 164 128 122 642.2 0 122 129 167 642.2 0 167 130 131 642.2 0 131 131 214 642.2 0 214 132 206 642.2 0 206 133 167 642.2 0 167 134 170 642.2 0 170 135 136 642.2 0 136 136 154 642.2 0 154 137 150 642.2 0 150 138 142 642.2 0 142 139 163 642.2 0 163 140 236 642.2 0 236 141 236 642.2 0 236 142 156 642.2 0 156 143 139 642.2 0 139 144 141 642.2 0 141 145 144 642.2 0 144 146 145 642.2 0 145 147 222 642.2 0 222 148 185 642.2 0 185 149 173 642.2 0 173 150 125 642.2 0 125 151 134 642.2 0 134 152 169 642.2 0 169 153 142 642.2 0 142 154 150 642.2 0 150 155 127 642.2 0 127 156 145 642.2 0 145 157 177 642.2 0 177 158 140 642.2 0 140 159 243 642.2 0 243 160 162 642.2 0 162 161 226 642.2 0 226 162 138 642.2 0 138 163 114 642.2 0 114 164 152 642.2 0 152 165 150 642.2 0 150 166 146 642.2 0 146 167 179 642.2 0 179 168 158 642.2 0 158 169 293 642.2 0 293 170 314 642.2 0 314 171 152 642.2 0 152 172 176 642.2 0 176 173 160 642.2 0 160 174 153 642.2 0 153 175 148 642.2 0 148 176 134 642.2 0 134 177 150 642.2 0 150 178 152 642.2 0 152 179 155 642.2 0 155 180 150 642.2 0 150 181 170 642.2 0 170 182 150 642.2 0 150 183 156 642.2 0 156 184 160 642.2 0 160 185 196 642.2 0 196 186 187 642.2 0 187 187 172 642.2 0 172 188 121 642.2 0 121 189 143 642.2 0 143 190 157 642.2 0 157 191 160 642.2 0 160 192 163 642.2 0 163 193 146 642.2 0 146 194 153 642.2 0 153 195 138 642.2 0 138 196 130 642.2 0 130 197 143 642.2 0 143 198 145 642.2 0 145 199 144 642.2 0 144 200 165 642.2 0 165 201 152 642.2 0 152 202 150 642.2 0 150 203 123 642.2 0 123 204 171 642.2 0 171 205 164 642.2 0 164 206 154 642.2 0 154 207 179 642.2 0 179 208 200 642.2 0 200 209 168 642.2 0 168 210 160 642.2 0 160 211 156 642.2 0 156 212 172 642.2 0 172 213 177 642.2 0 177 214 158 642.2 0 158 215 144 642.2 0 144 216 139 642.2 0 139 217 132 642.2 0 132 218 152 642.2 0 152 219 147 642.2 0 147 220 145 642.2 0 145 221 174 642.2 0 174 222 193 642.2 0 193 223 133 642.2 0 133 224 172 642.2 0 172 225 140 642.2 0 140 226 122 642.2 0 122 227 159 642.2 0 159 228 146 642.2 0 146 229 137 642.2 0 137 230 144 642.2 0 144 231 139 642.2 0 139 232 194 642.2 0 194 233 177 642.2 0 177 234 173 642.2 0 173 235 1254 642.2 0 1254 236 165 642.2 0 165 237 746 642.2 0 746 238 139 642.2 0 139 239 141 642.2 0 141 240 149 642.2 0 149 241 251 642.2 0 251 242 201 642.2 0 201 243 179 642.2 0 179 244 118 642.2 0 118 245 92 642.2 0 92 246 118 642.2 0 118 247 102 642.2 0 102 248 359 642.2 0 359 249 157 642.2 0 157 250 158 642.2 0 158 251 117 642.2 0 117 252 93 642.2 0 93 253 107 642.2 0 107 254 134 642.2 0 134 255 145 642.2 0 145 256 172 642.2 0 172 257 169 642.2 0 169 258 180 642.2 0 180 259 174 642.2 0 174 260 205 642.2 0 205 261 203 642.2 0 203 262 132 642.2 0 132 263 96 642.2 0 96 264 132 642.2 0 132 265 120 642.2 0 120 266 98 642.2 0 98 267 116 642.2 0 116 268 85 642.2 0 85 269 85 642.2 0 85 270 120 642.2 0 120 271 105 642.2 0 105 272 112 642.2 0 112 273 131 642.2 0 131 274 133 642.2 0 133 275 111 642.2 0 111 276 94 642.2 0 94 277 112 642.2 0 112 278 124 642.2 0 124 279 123 642.2 0 123 280 108 642.2 0 108 281 112 642.2 0 112 282 137 642.2 0 137 283 132 642.2 0 132 284 124 642.2 0 124 285 128 642.2 0 128 286 109 642.2 0 109 287 123 642.2 0 123 288 117 642.2 0 117 289 104 642.2 0 104 290 94 642.2 0 94 291 110 642.2 0 110 292 103 642.2 0 103 293 120 642.2 0 120 294 122 642.2 0 122 295 122 642.2 0 122 296 105 642.2 0 105 297 103 642.2 0 103 298 106 642.2 0 106 299 121 642.2 0 121 300 123 642.2 0 123 301 140 642.2 0 140 Finished in 1.85 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 45,436 Reads with adapters: 4,214 (9.3%) Reads that were too short: 38 (0.1%) Reads written (passing filters): 4,176 (9.2%) Total basepairs processed: 10,056,424 bp Total written (filtered): 1,142,324 bp (11.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4214 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 3 16 39 0.0 3 0 39 17 4069 0.0 3 3991 77 1 18 18 0.0 3 5 12 1 19 2 0.0 3 0 0 2 23 1 0.0 3 0 0 0 1 27 1 0.0 3 1 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 1 52 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 81 8 0.0 3 0 0 1 7 84 2 0.0 3 0 0 0 2 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 2 0.0 3 1 0 0 1 93 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 142 1 0.0 3 1 143 2 0.0 3 0 0 0 2 148 7 0.0 3 0 0 0 7 169 34 0.0 3 0 0 0 34 175 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 4,176 Reads with adapters: 6 (0.1%) Reads written (passing filters): 4,176 (100.0%) Total basepairs processed: 1,142,324 bp Total written (filtered): 1,142,304 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.3% C: 16.7% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 65.2 0 4 4 2 16.3 0 2