This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 70.28 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 321,273 (12.2%) Reads that were too short: 13,590 (0.5%) Reads written (passing filters): 307,683 (11.7%) Total basepairs processed: 791,719,999 bp Total written (filtered): 81,020,220 bp (10.2%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 321273 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1544 642.2 0 1544 7 5885 642.2 0 5885 8 115223 642.2 0 115223 9 101718 642.2 0 101718 10 169 642.2 0 169 11 56 642.2 0 56 12 57 642.2 0 57 13 49 642.2 0 49 14 46 642.2 0 46 15 37 642.2 0 37 16 57 642.2 0 57 17 41 642.2 0 41 18 48 642.2 0 48 19 62 642.2 0 62 20 58 642.2 0 58 21 63 642.2 0 63 22 62 642.2 0 62 23 90 642.2 0 90 24 106 642.2 0 106 25 129 642.2 0 129 26 102 642.2 0 102 27 68 642.2 0 68 28 60 642.2 0 60 29 50 642.2 0 50 30 82 642.2 0 82 31 86 642.2 0 86 32 68 642.2 0 68 33 67 642.2 0 67 34 137 642.2 0 137 35 124 642.2 0 124 36 210 642.2 0 210 37 228 642.2 0 228 38 57 642.2 0 57 39 95 642.2 0 95 40 47 642.2 0 47 41 57 642.2 0 57 42 78 642.2 0 78 43 49 642.2 0 49 44 50 642.2 0 50 45 72 642.2 0 72 46 89 642.2 0 89 47 379 642.2 0 379 48 94 642.2 0 94 49 124 642.2 0 124 50 57 642.2 0 57 51 80 642.2 0 80 52 68 642.2 0 68 53 138 642.2 0 138 54 215 642.2 0 215 55 236 642.2 0 236 56 104 642.2 0 104 57 73 642.2 0 73 58 51 642.2 0 51 59 30 642.2 0 30 60 74 642.2 0 74 61 112 642.2 0 112 62 92 642.2 0 92 63 106 642.2 0 106 64 213 642.2 0 213 65 79 642.2 0 79 66 83 642.2 0 83 67 91 642.2 0 91 68 79 642.2 0 79 69 101 642.2 0 101 70 80 642.2 0 80 71 78 642.2 0 78 72 96 642.2 0 96 73 82 642.2 0 82 74 160 642.2 0 160 75 150 642.2 0 150 76 254 642.2 0 254 77 760 642.2 0 760 78 723 642.2 0 723 79 902 642.2 0 902 80 526 642.2 0 526 81 5054 642.2 0 5054 82 7009 642.2 0 7009 83 4552 642.2 0 4552 84 140 642.2 0 140 85 301 642.2 0 301 86 270 642.2 0 270 87 594 642.2 0 594 88 87 642.2 0 87 89 164 642.2 0 164 90 35 642.2 0 35 91 39 642.2 0 39 92 57 642.2 0 57 93 87 642.2 0 87 94 97 642.2 0 97 95 81 642.2 0 81 96 62 642.2 0 62 97 57 642.2 0 57 98 76 642.2 0 76 99 53 642.2 0 53 100 64 642.2 0 64 101 83 642.2 0 83 102 187 642.2 0 187 103 83 642.2 0 83 104 174 642.2 0 174 105 51 642.2 0 51 106 77 642.2 0 77 107 62 642.2 0 62 108 112 642.2 0 112 109 116 642.2 0 116 110 74 642.2 0 74 111 122 642.2 0 122 112 130 642.2 0 130 113 78 642.2 0 78 114 86 642.2 0 86 115 102 642.2 0 102 116 94 642.2 0 94 117 159 642.2 0 159 118 245 642.2 0 245 119 568 642.2 0 568 120 372 642.2 0 372 121 942 642.2 0 942 122 12080 642.2 0 12080 123 6104 642.2 0 6104 124 14748 642.2 0 14748 125 190 642.2 0 190 126 68 642.2 0 68 127 76 642.2 0 76 128 225 642.2 0 225 129 70 642.2 0 70 130 87 642.2 0 87 131 50 642.2 0 50 132 64 642.2 0 64 133 120 642.2 0 120 134 78 642.2 0 78 135 101 642.2 0 101 136 67 642.2 0 67 137 57 642.2 0 57 138 64 642.2 0 64 139 76 642.2 0 76 140 68 642.2 0 68 141 78 642.2 0 78 142 141 642.2 0 141 143 170 642.2 0 170 144 598 642.2 0 598 145 200 642.2 0 200 146 356 642.2 0 356 147 135 642.2 0 135 148 391 642.2 0 391 149 357 642.2 0 357 150 120 642.2 0 120 151 428 642.2 0 428 152 85 642.2 0 85 153 77 642.2 0 77 154 97 642.2 0 97 155 80 642.2 0 80 156 61 642.2 0 61 157 47 642.2 0 47 158 67 642.2 0 67 159 60 642.2 0 60 160 80 642.2 0 80 161 61 642.2 0 61 162 64 642.2 0 64 163 49 642.2 0 49 164 48 642.2 0 48 165 61 642.2 0 61 166 62 642.2 0 62 167 158 642.2 0 158 168 93 642.2 0 93 169 64 642.2 0 64 170 83 642.2 0 83 171 52 642.2 0 52 172 63 642.2 0 63 173 62 642.2 0 62 174 57 642.2 0 57 175 84 642.2 0 84 176 53 642.2 0 53 177 75 642.2 0 75 178 124 642.2 0 124 179 85 642.2 0 85 180 118 642.2 0 118 181 563 642.2 0 563 182 4605 642.2 0 4605 183 2851 642.2 0 2851 184 1924 642.2 0 1924 185 105 642.2 0 105 186 71 642.2 0 71 187 74 642.2 0 74 188 74 642.2 0 74 189 55 642.2 0 55 190 73 642.2 0 73 191 41 642.2 0 41 192 61 642.2 0 61 193 90 642.2 0 90 194 166 642.2 0 166 195 147 642.2 0 147 196 78 642.2 0 78 197 54 642.2 0 54 198 141 642.2 0 141 199 152 642.2 0 152 200 90 642.2 0 90 201 69 642.2 0 69 202 57 642.2 0 57 203 45 642.2 0 45 204 52 642.2 0 52 205 101 642.2 0 101 206 353 642.2 0 353 207 63 642.2 0 63 208 45 642.2 0 45 209 86 642.2 0 86 210 87 642.2 0 87 211 86 642.2 0 86 212 71 642.2 0 71 213 54 642.2 0 54 214 56 642.2 0 56 215 41 642.2 0 41 216 35 642.2 0 35 217 55 642.2 0 55 218 55 642.2 0 55 219 77 642.2 0 77 220 60 642.2 0 60 221 83 642.2 0 83 222 48 642.2 0 48 223 56 642.2 0 56 224 57 642.2 0 57 225 50 642.2 0 50 226 113 642.2 0 113 227 72 642.2 0 72 228 114 642.2 0 114 229 78 642.2 0 78 230 89 642.2 0 89 231 66 642.2 0 66 232 46 642.2 0 46 233 61 642.2 0 61 234 58 642.2 0 58 235 65 642.2 0 65 236 68 642.2 0 68 237 55 642.2 0 55 238 84 642.2 0 84 239 81 642.2 0 81 240 235 642.2 0 235 241 365 642.2 0 365 242 158 642.2 0 158 243 88 642.2 0 88 244 56 642.2 0 56 245 63 642.2 0 63 246 42 642.2 0 42 247 80 642.2 0 80 248 50 642.2 0 50 249 55 642.2 0 55 250 58 642.2 0 58 251 63 642.2 0 63 252 75 642.2 0 75 253 116 642.2 0 116 254 109 642.2 0 109 255 104 642.2 0 104 256 100 642.2 0 100 257 88 642.2 0 88 258 96 642.2 0 96 259 92 642.2 0 92 260 101 642.2 0 101 261 142 642.2 0 142 262 87 642.2 0 87 263 88 642.2 0 88 264 78 642.2 0 78 265 79 642.2 0 79 266 1010 642.2 0 1010 267 404 642.2 0 404 268 1215 642.2 0 1215 269 257 642.2 0 257 270 71 642.2 0 71 271 52 642.2 0 52 272 81 642.2 0 81 273 56 642.2 0 56 274 166 642.2 0 166 275 173 642.2 0 173 276 180 642.2 0 180 277 158 642.2 0 158 278 299 642.2 0 299 279 263 642.2 0 263 280 132 642.2 0 132 281 207 642.2 0 207 282 78 642.2 0 78 283 114 642.2 0 114 284 49 642.2 0 49 285 58 642.2 0 58 286 106 642.2 0 106 287 165 642.2 0 165 288 724 642.2 0 724 289 207 642.2 0 207 290 273 642.2 0 273 291 85 642.2 0 85 292 125 642.2 0 125 293 141 642.2 0 141 294 205 642.2 0 205 295 119 642.2 0 119 296 214 642.2 0 214 297 217 642.2 0 217 298 151 642.2 0 151 299 94 642.2 0 94 300 126 642.2 0 126 301 124 642.2 0 124 Finished in 14.71 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 307,683 Reads with adapters: 108,270 (35.2%) Reads that were too short: 154 (0.1%) Reads written (passing filters): 108,116 (35.1%) Total basepairs processed: 81,020,220 bp Total written (filtered): 29,301,407 bp (36.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 108270 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 21 0.0 3 0 0 0 14 7 18 102 0.0 3 0 0 58 30 14 19 1958 0.0 3 0 1595 151 143 69 20 102864 0.0 4 93759 7015 1490 449 151 21 235 0.0 4 28 167 20 11 9 22 1257 0.0 4 1119 82 32 10 14 23 609 0.0 4 3 2 1 385 218 24 2 0.0 4 0 0 0 1 1 25 5 0.0 4 1 0 0 3 1 27 2 0.0 4 1 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 1 47 2 0.0 4 2 50 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 1 63 1 0.0 4 0 0 0 0 1 66 1 0.0 4 1 75 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 81 2 0.0 4 0 0 0 0 2 82 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 93 2 0.0 4 0 0 0 0 2 94 1 0.0 4 0 0 0 0 1 99 5 0.0 4 0 0 0 0 5 100 3 0.0 4 0 0 0 0 3 101 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 14 0.0 4 0 0 0 0 14 127 1009 0.0 4 0 0 0 0 1009 128 6 0.0 4 0 0 0 0 6 129 4 0.0 4 0 0 0 0 4 130 5 0.0 4 0 0 0 0 5 132 1 0.0 4 1 135 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 147 2 0.0 4 0 0 0 0 2 148 4 0.0 4 0 0 0 0 4 149 11 0.0 4 0 0 0 2 9 150 6 0.0 4 0 0 0 0 6 153 1 0.0 4 0 0 0 0 1 156 2 0.0 4 0 0 0 0 2 157 1 0.0 4 0 0 0 0 1 179 2 0.0 4 0 0 0 0 2 182 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 225 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 5 0.0 4 0 0 0 2 3 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 6 0.0 4 0 0 0 0 6 235 10 0.0 4 0 0 0 0 10 237 8 0.0 4 0 0 0 0 8 251 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 282 40 0.0 4 0 0 0 0 40 283 7 0.0 4 0 0 0 1 6 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 1 292 2 0.0 4 0 0 0 1 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.11 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 108,116 Reads with adapters: 43,182 (39.9%) Reads written (passing filters): 108,116 (100.0%) Total basepairs processed: 29,301,407 bp Total written (filtered): 28,894,137 bp (98.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 43182 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 5.4% G: 91.5% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 37 1689.3 0 37 4 31 422.3 0 31 5 5 105.6 0 5 6 4 26.4 0 4 7 5 6.6 0 5 8 30 1.6 0 30 9 34983 0.4 0 2991 31992 10 6124 0.1 1 466 5658 11 1662 0.0 1 72 1590 12 233 0.0 1 2 231 13 19 0.0 1 0 19 14 2 0.0 1 0 2 30 1 0.0 1 1 60 1 0.0 1 1 85 1 0.0 1 0 1 110 1 0.0 1 1 122 1 0.0 1 1 139 1 0.0 1 1 150 1 0.0 1 1 157 1 0.0 1 1 169 3 0.0 1 3 172 1 0.0 1 1 189 1 0.0 1 0 1 208 14 0.0 1 12 2 209 1 0.0 1 0 1 226 13 0.0 1 11 2 227 1 0.0 1 1 228 2 0.0 1 2 268 1 0.0 1 1 269 1 0.0 1 0 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.