This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 70.44 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 342,011 (13.0%) Reads that were too short: 13,840 (0.5%) Reads written (passing filters): 328,171 (12.5%) Total basepairs processed: 791,719,999 bp Total written (filtered): 86,108,546 bp (10.9%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 342011 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1270 642.2 0 1270 7 4371 642.2 0 4371 8 111230 642.2 0 111230 9 120464 642.2 0 120464 10 202 642.2 0 202 11 64 642.2 0 64 12 46 642.2 0 46 13 69 642.2 0 69 14 53 642.2 0 53 15 45 642.2 0 45 16 76 642.2 0 76 17 55 642.2 0 55 18 78 642.2 0 78 19 69 642.2 0 69 20 49 642.2 0 49 21 61 642.2 0 61 22 64 642.2 0 64 23 74 642.2 0 74 24 60 642.2 0 60 25 54 642.2 0 54 26 60 642.2 0 60 27 61 642.2 0 61 28 68 642.2 0 68 29 64 642.2 0 64 30 53 642.2 0 53 31 85 642.2 0 85 32 72 642.2 0 72 33 84 642.2 0 84 34 91 642.2 0 91 35 93 642.2 0 93 36 100 642.2 0 100 37 278 642.2 0 278 38 78 642.2 0 78 39 132 642.2 0 132 40 67 642.2 0 67 41 91 642.2 0 91 42 88 642.2 0 88 43 75 642.2 0 75 44 79 642.2 0 79 45 79 642.2 0 79 46 106 642.2 0 106 47 428 642.2 0 428 48 117 642.2 0 117 49 131 642.2 0 131 50 88 642.2 0 88 51 92 642.2 0 92 52 95 642.2 0 95 53 128 642.2 0 128 54 257 642.2 0 257 55 144 642.2 0 144 56 110 642.2 0 110 57 79 642.2 0 79 58 67 642.2 0 67 59 79 642.2 0 79 60 71 642.2 0 71 61 135 642.2 0 135 62 91 642.2 0 91 63 167 642.2 0 167 64 269 642.2 0 269 65 65 642.2 0 65 66 107 642.2 0 107 67 101 642.2 0 101 68 89 642.2 0 89 69 119 642.2 0 119 70 103 642.2 0 103 71 120 642.2 0 120 72 113 642.2 0 113 73 90 642.2 0 90 74 169 642.2 0 169 75 166 642.2 0 166 76 320 642.2 0 320 77 980 642.2 0 980 78 777 642.2 0 777 79 881 642.2 0 881 80 411 642.2 0 411 81 6018 642.2 0 6018 82 8518 642.2 0 8518 83 3592 642.2 0 3592 84 137 642.2 0 137 85 195 642.2 0 195 86 144 642.2 0 144 87 229 642.2 0 229 88 101 642.2 0 101 89 167 642.2 0 167 90 62 642.2 0 62 91 71 642.2 0 71 92 90 642.2 0 90 93 111 642.2 0 111 94 97 642.2 0 97 95 91 642.2 0 91 96 90 642.2 0 90 97 78 642.2 0 78 98 134 642.2 0 134 99 109 642.2 0 109 100 56 642.2 0 56 101 127 642.2 0 127 102 125 642.2 0 125 103 87 642.2 0 87 104 148 642.2 0 148 105 75 642.2 0 75 106 68 642.2 0 68 107 105 642.2 0 105 108 149 642.2 0 149 109 155 642.2 0 155 110 93 642.2 0 93 111 159 642.2 0 159 112 180 642.2 0 180 113 102 642.2 0 102 114 109 642.2 0 109 115 173 642.2 0 173 116 155 642.2 0 155 117 161 642.2 0 161 118 267 642.2 0 267 119 328 642.2 0 328 120 316 642.2 0 316 121 625 642.2 0 625 122 13172 642.2 0 13172 123 6743 642.2 0 6743 124 16980 642.2 0 16980 125 132 642.2 0 132 126 99 642.2 0 99 127 96 642.2 0 96 128 224 642.2 0 224 129 103 642.2 0 103 130 97 642.2 0 97 131 70 642.2 0 70 132 63 642.2 0 63 133 100 642.2 0 100 134 72 642.2 0 72 135 113 642.2 0 113 136 59 642.2 0 59 137 73 642.2 0 73 138 91 642.2 0 91 139 72 642.2 0 72 140 80 642.2 0 80 141 71 642.2 0 71 142 141 642.2 0 141 143 198 642.2 0 198 144 608 642.2 0 608 145 224 642.2 0 224 146 209 642.2 0 209 147 165 642.2 0 165 148 204 642.2 0 204 149 276 642.2 0 276 150 117 642.2 0 117 151 214 642.2 0 214 152 72 642.2 0 72 153 67 642.2 0 67 154 87 642.2 0 87 155 80 642.2 0 80 156 62 642.2 0 62 157 55 642.2 0 55 158 62 642.2 0 62 159 54 642.2 0 54 160 86 642.2 0 86 161 79 642.2 0 79 162 80 642.2 0 80 163 75 642.2 0 75 164 81 642.2 0 81 165 85 642.2 0 85 166 59 642.2 0 59 167 184 642.2 0 184 168 123 642.2 0 123 169 77 642.2 0 77 170 73 642.2 0 73 171 65 642.2 0 65 172 80 642.2 0 80 173 74 642.2 0 74 174 55 642.2 0 55 175 85 642.2 0 85 176 78 642.2 0 78 177 90 642.2 0 90 178 160 642.2 0 160 179 113 642.2 0 113 180 131 642.2 0 131 181 486 642.2 0 486 182 5723 642.2 0 5723 183 3727 642.2 0 3727 184 2307 642.2 0 2307 185 79 642.2 0 79 186 88 642.2 0 88 187 77 642.2 0 77 188 79 642.2 0 79 189 49 642.2 0 49 190 77 642.2 0 77 191 55 642.2 0 55 192 79 642.2 0 79 193 65 642.2 0 65 194 69 642.2 0 69 195 77 642.2 0 77 196 50 642.2 0 50 197 59 642.2 0 59 198 68 642.2 0 68 199 119 642.2 0 119 200 116 642.2 0 116 201 70 642.2 0 70 202 82 642.2 0 82 203 84 642.2 0 84 204 82 642.2 0 82 205 89 642.2 0 89 206 448 642.2 0 448 207 61 642.2 0 61 208 73 642.2 0 73 209 67 642.2 0 67 210 68 642.2 0 68 211 72 642.2 0 72 212 77 642.2 0 77 213 79 642.2 0 79 214 89 642.2 0 89 215 81 642.2 0 81 216 55 642.2 0 55 217 63 642.2 0 63 218 76 642.2 0 76 219 98 642.2 0 98 220 93 642.2 0 93 221 87 642.2 0 87 222 63 642.2 0 63 223 70 642.2 0 70 224 82 642.2 0 82 225 94 642.2 0 94 226 82 642.2 0 82 227 74 642.2 0 74 228 86 642.2 0 86 229 66 642.2 0 66 230 65 642.2 0 65 231 54 642.2 0 54 232 54 642.2 0 54 233 63 642.2 0 63 234 73 642.2 0 73 235 78 642.2 0 78 236 66 642.2 0 66 237 99 642.2 0 99 238 103 642.2 0 103 239 67 642.2 0 67 240 229 642.2 0 229 241 349 642.2 0 349 242 179 642.2 0 179 243 77 642.2 0 77 244 72 642.2 0 72 245 62 642.2 0 62 246 57 642.2 0 57 247 61 642.2 0 61 248 77 642.2 0 77 249 68 642.2 0 68 250 71 642.2 0 71 251 96 642.2 0 96 252 109 642.2 0 109 253 212 642.2 0 212 254 94 642.2 0 94 255 107 642.2 0 107 256 129 642.2 0 129 257 84 642.2 0 84 258 104 642.2 0 104 259 74 642.2 0 74 260 87 642.2 0 87 261 108 642.2 0 108 262 92 642.2 0 92 263 84 642.2 0 84 264 85 642.2 0 85 265 109 642.2 0 109 266 1397 642.2 0 1397 267 420 642.2 0 420 268 1536 642.2 0 1536 269 198 642.2 0 198 270 66 642.2 0 66 271 76 642.2 0 76 272 80 642.2 0 80 273 75 642.2 0 75 274 147 642.2 0 147 275 176 642.2 0 176 276 214 642.2 0 214 277 85 642.2 0 85 278 298 642.2 0 298 279 108 642.2 0 108 280 73 642.2 0 73 281 154 642.2 0 154 282 103 642.2 0 103 283 127 642.2 0 127 284 59 642.2 0 59 285 71 642.2 0 71 286 97 642.2 0 97 287 117 642.2 0 117 288 315 642.2 0 315 289 177 642.2 0 177 290 125 642.2 0 125 291 73 642.2 0 73 292 100 642.2 0 100 293 115 642.2 0 115 294 147 642.2 0 147 295 108 642.2 0 108 296 152 642.2 0 152 297 245 642.2 0 245 298 159 642.2 0 159 299 96 642.2 0 96 300 101 642.2 0 101 301 111 642.2 0 111 Finished in 13.70 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 328,171 Reads with adapters: 112,053 (34.1%) Reads that were too short: 168 (0.1%) Reads written (passing filters): 111,885 (34.1%) Total basepairs processed: 86,108,546 bp Total written (filtered): 30,801,381 bp (35.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 112053 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 21 0.0 3 0 0 21 16 524 0.0 3 0 514 10 17 110416 0.0 3 108391 1953 66 6 18 272 0.0 3 10 260 2 19 45 0.0 3 0 0 41 4 20 19 0.0 3 0 0 0 19 21 1 0.0 3 1 22 2 0.0 3 0 0 0 2 24 1 0.0 3 1 25 123 0.0 3 0 0 111 12 28 5 0.0 3 0 0 0 5 29 1 0.0 3 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 163 0.0 3 0 0 0 163 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 1 49 43 0.0 3 0 0 0 43 50 156 0.0 3 0 0 0 156 51 59 0.0 3 0 0 1 58 52 9 0.0 3 0 0 0 9 53 19 0.0 3 0 0 1 18 54 11 0.0 3 0 0 0 11 66 1 0.0 3 0 0 0 1 70 2 0.0 3 1 0 0 1 73 1 0.0 3 0 0 0 1 75 3 0.0 3 1 0 0 2 76 3 0.0 3 0 0 0 3 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 87 3 0.0 3 1 0 0 2 93 1 0.0 3 1 94 1 0.0 3 1 101 1 0.0 3 0 0 0 1 106 5 0.0 3 0 0 0 5 110 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 161 6 0.0 3 0 0 0 6 162 16 0.0 3 0 0 1 15 163 11 0.0 3 0 0 0 11 164 15 0.0 3 0 0 0 15 165 36 0.0 3 0 0 3 33 166 10 0.0 3 0 0 0 10 174 1 0.0 3 0 0 0 1 179 1 0.0 3 1 185 3 0.0 3 0 0 0 3 187 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 221 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 287 1 0.0 3 1 291 1 0.0 3 0 0 0 1 292 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.01 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 111,885 Reads with adapters: 300 (0.3%) Reads written (passing filters): 111,885 (100.0%) Total basepairs processed: 30,801,381 bp Total written (filtered): 30,800,344 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 300 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.3% C: 10.3% G: 42.0% T: 2.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 172 1748.2 0 172 4 125 437.1 0 125 5 1 109.3 0 1 6 1 27.3 0 1 10 1 0.1 1 0 1