This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.97 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 68,814 (2.6%) Reads that were too short: 18,560 (0.7%) Reads written (passing filters): 50,254 (1.9%) Total basepairs processed: 791,719,999 bp Total written (filtered): 11,097,328 bp (1.4%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 68814 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 351 642.2 0 351 7 3100 642.2 0 3100 8 2698 642.2 0 2698 9 81 642.2 0 81 10 136 642.2 0 136 11 32 642.2 0 32 12 59 642.2 0 59 13 53 642.2 0 53 14 35 642.2 0 35 15 23 642.2 0 23 16 11 642.2 0 11 17 29 642.2 0 29 18 38 642.2 0 38 19 21 642.2 0 21 20 31 642.2 0 31 21 69 642.2 0 69 22 42 642.2 0 42 23 43 642.2 0 43 24 36 642.2 0 36 25 48 642.2 0 48 26 38 642.2 0 38 27 47 642.2 0 47 28 65 642.2 0 65 29 26 642.2 0 26 30 123 642.2 0 123 31 48 642.2 0 48 32 53 642.2 0 53 33 36 642.2 0 36 34 35 642.2 0 35 35 23 642.2 0 23 36 41 642.2 0 41 37 50 642.2 0 50 38 75 642.2 0 75 39 34 642.2 0 34 40 59 642.2 0 59 41 56 642.2 0 56 42 61 642.2 0 61 43 147 642.2 0 147 44 143 642.2 0 143 45 24 642.2 0 24 46 36 642.2 0 36 47 53 642.2 0 53 48 110 642.2 0 110 49 91 642.2 0 91 50 133 642.2 0 133 51 137 642.2 0 137 52 121 642.2 0 121 53 187 642.2 0 187 54 921 642.2 0 921 55 8357 642.2 0 8357 56 6591 642.2 0 6591 57 5360 642.2 0 5360 58 68 642.2 0 68 59 49 642.2 0 49 60 42 642.2 0 42 61 65 642.2 0 65 62 42 642.2 0 42 63 323 642.2 0 323 64 38 642.2 0 38 65 62 642.2 0 62 66 42 642.2 0 42 67 34 642.2 0 34 68 30 642.2 0 30 69 61 642.2 0 61 70 41 642.2 0 41 71 50 642.2 0 50 72 49 642.2 0 49 73 48 642.2 0 48 74 79 642.2 0 79 75 43 642.2 0 43 76 108 642.2 0 108 77 55 642.2 0 55 78 49 642.2 0 49 79 46 642.2 0 46 80 42 642.2 0 42 81 102 642.2 0 102 82 69 642.2 0 69 83 65 642.2 0 65 84 85 642.2 0 85 85 693 642.2 0 693 86 443 642.2 0 443 87 343 642.2 0 343 88 78 642.2 0 78 89 86 642.2 0 86 90 44 642.2 0 44 91 64 642.2 0 64 92 40 642.2 0 40 93 44 642.2 0 44 94 43 642.2 0 43 95 37 642.2 0 37 96 62 642.2 0 62 97 54 642.2 0 54 98 50 642.2 0 50 99 58 642.2 0 58 100 84 642.2 0 84 101 126 642.2 0 126 102 532 642.2 0 532 103 497 642.2 0 497 104 413 642.2 0 413 105 61 642.2 0 61 106 69 642.2 0 69 107 78 642.2 0 78 108 70 642.2 0 70 109 52 642.2 0 52 110 41 642.2 0 41 111 50 642.2 0 50 112 71 642.2 0 71 113 65 642.2 0 65 114 62 642.2 0 62 115 55 642.2 0 55 116 45 642.2 0 45 117 235 642.2 0 235 118 794 642.2 0 794 119 1564 642.2 0 1564 120 131 642.2 0 131 121 50 642.2 0 50 122 110 642.2 0 110 123 51 642.2 0 51 124 77 642.2 0 77 125 70 642.2 0 70 126 52 642.2 0 52 127 200 642.2 0 200 128 61 642.2 0 61 129 48 642.2 0 48 130 61 642.2 0 61 131 71 642.2 0 71 132 65 642.2 0 65 133 131 642.2 0 131 134 81 642.2 0 81 135 75 642.2 0 75 136 71 642.2 0 71 137 358 642.2 0 358 138 309 642.2 0 309 139 521 642.2 0 521 140 163 642.2 0 163 141 69 642.2 0 69 142 52 642.2 0 52 143 46 642.2 0 46 144 37 642.2 0 37 145 45 642.2 0 45 146 48 642.2 0 48 147 87 642.2 0 87 148 100 642.2 0 100 149 58 642.2 0 58 150 61 642.2 0 61 151 75 642.2 0 75 152 72 642.2 0 72 153 76 642.2 0 76 154 77 642.2 0 77 155 85 642.2 0 85 156 201 642.2 0 201 157 221 642.2 0 221 158 252 642.2 0 252 159 221 642.2 0 221 160 70 642.2 0 70 161 77 642.2 0 77 162 118 642.2 0 118 163 90 642.2 0 90 164 340 642.2 0 340 165 102 642.2 0 102 166 276 642.2 0 276 167 330 642.2 0 330 168 152 642.2 0 152 169 375 642.2 0 375 170 124 642.2 0 124 171 71 642.2 0 71 172 43 642.2 0 43 173 76 642.2 0 76 174 72 642.2 0 72 175 70 642.2 0 70 176 171 642.2 0 171 177 45 642.2 0 45 178 63 642.2 0 63 179 62 642.2 0 62 180 61 642.2 0 61 181 91 642.2 0 91 182 108 642.2 0 108 183 122 642.2 0 122 184 156 642.2 0 156 185 109 642.2 0 109 186 101 642.2 0 101 187 488 642.2 0 488 188 544 642.2 0 544 189 572 642.2 0 572 190 443 642.2 0 443 191 95 642.2 0 95 192 89 642.2 0 89 193 49 642.2 0 49 194 103 642.2 0 103 195 82 642.2 0 82 196 68 642.2 0 68 197 101 642.2 0 101 198 59 642.2 0 59 199 113 642.2 0 113 200 107 642.2 0 107 201 120 642.2 0 120 202 60 642.2 0 60 203 91 642.2 0 91 204 106 642.2 0 106 205 78 642.2 0 78 206 125 642.2 0 125 207 102 642.2 0 102 208 99 642.2 0 99 209 232 642.2 0 232 210 70 642.2 0 70 211 79 642.2 0 79 212 47 642.2 0 47 213 56 642.2 0 56 214 71 642.2 0 71 215 140 642.2 0 140 216 119 642.2 0 119 217 85 642.2 0 85 218 113 642.2 0 113 219 367 642.2 0 367 220 616 642.2 0 616 221 3658 642.2 0 3658 222 76 642.2 0 76 223 95 642.2 0 95 224 69 642.2 0 69 225 73 642.2 0 73 226 52 642.2 0 52 227 40 642.2 0 40 228 57 642.2 0 57 229 65 642.2 0 65 230 52 642.2 0 52 231 81 642.2 0 81 232 63 642.2 0 63 233 103 642.2 0 103 234 251 642.2 0 251 235 113 642.2 0 113 236 124 642.2 0 124 237 85 642.2 0 85 238 393 642.2 0 393 239 192 642.2 0 192 240 185 642.2 0 185 241 136 642.2 0 136 242 88 642.2 0 88 243 66 642.2 0 66 244 60 642.2 0 60 245 55 642.2 0 55 246 104 642.2 0 104 247 102 642.2 0 102 248 548 642.2 0 548 249 93 642.2 0 93 250 116 642.2 0 116 251 102 642.2 0 102 252 76 642.2 0 76 253 46 642.2 0 46 254 40 642.2 0 40 255 41 642.2 0 41 256 64 642.2 0 64 257 120 642.2 0 120 258 447 642.2 0 447 259 193 642.2 0 193 260 225 642.2 0 225 261 245 642.2 0 245 262 214 642.2 0 214 263 77 642.2 0 77 264 76 642.2 0 76 265 70 642.2 0 70 266 77 642.2 0 77 267 161 642.2 0 161 268 91 642.2 0 91 269 139 642.2 0 139 270 101 642.2 0 101 271 47 642.2 0 47 272 75 642.2 0 75 273 101 642.2 0 101 274 122 642.2 0 122 275 93 642.2 0 93 276 97 642.2 0 97 277 66 642.2 0 66 278 321 642.2 0 321 279 237 642.2 0 237 280 239 642.2 0 239 281 118 642.2 0 118 282 123 642.2 0 123 283 197 642.2 0 197 284 103 642.2 0 103 285 160 642.2 0 160 286 98 642.2 0 98 287 170 642.2 0 170 288 276 642.2 0 276 289 188 642.2 0 188 290 180 642.2 0 180 291 158 642.2 0 158 292 168 642.2 0 168 293 140 642.2 0 140 294 253 642.2 0 253 295 125 642.2 0 125 296 234 642.2 0 234 297 157 642.2 0 157 298 541 642.2 0 541 299 528 642.2 0 528 300 455 642.2 0 455 301 334 642.2 0 334 Finished in 2.29 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 50,254 Reads with adapters: 3,027 (6.0%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 3,003 (6.0%) Total basepairs processed: 11,097,328 bp Total written (filtered): 816,138 bp (7.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3027 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 19 0.0 3 0 11 5 2 1 20 2712 0.0 4 2497 177 28 8 2 21 12 0.0 4 1 8 0 0 3 22 30 0.0 4 17 9 2 2 23 194 0.0 4 164 22 4 2 2 26 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 45 1 0.0 4 1 46 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 1 56 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 78 1 0.0 4 1 79 1 0.0 4 1 114 1 0.0 4 0 0 0 1 116 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 127 24 0.0 4 0 0 0 0 24 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 132 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 1 169 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 210 2 0.0 4 0 0 0 1 1 228 5 0.0 4 0 0 0 0 5 231 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.10 s (33 us/read; 1.80 M reads/minute). === Summary === Total reads processed: 3,003 Reads with adapters: 797 (26.5%) Reads written (passing filters): 3,003 (100.0%) Total basepairs processed: 816,138 bp Total written (filtered): 761,023 bp (93.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 797 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 28.9% G: 67.8% T: 1.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 46.9 0 2 4 3 11.7 0 3 8 1 0.0 0 1 9 20 0.0 0 6 14 10 63 0.0 1 5 58 11 522 0.0 1 18 504 12 3 0.0 1 0 3 14 1 0.0 1 0 1 150 1 0.0 1 0 1 266 1 0.0 1 1 267 172 0.0 1 165 7 268 5 0.0 1 5 269 3 0.0 1 2 1