This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.16 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,630,299 Reads with adapters: 54,547 (2.1%) Reads that were too short: 10,213 (0.4%) Reads written (passing filters): 44,334 (1.7%) Total basepairs processed: 791,719,999 bp Total written (filtered): 10,421,255 bp (1.3%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 54547 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 327 642.2 0 327 7 2895 642.2 0 2895 8 3401 642.2 0 3401 9 78 642.2 0 78 10 47 642.2 0 47 11 21 642.2 0 21 12 32 642.2 0 32 13 14 642.2 0 14 14 11 642.2 0 11 15 11 642.2 0 11 16 15 642.2 0 15 17 17 642.2 0 17 18 45 642.2 0 45 19 18 642.2 0 18 20 22 642.2 0 22 21 43 642.2 0 43 22 17 642.2 0 17 23 25 642.2 0 25 24 36 642.2 0 36 25 26 642.2 0 26 26 20 642.2 0 20 27 36 642.2 0 36 28 75 642.2 0 75 29 26 642.2 0 26 30 92 642.2 0 92 31 36 642.2 0 36 32 30 642.2 0 30 33 23 642.2 0 23 34 22 642.2 0 22 35 24 642.2 0 24 36 38 642.2 0 38 37 41 642.2 0 41 38 107 642.2 0 107 39 37 642.2 0 37 40 48 642.2 0 48 41 39 642.2 0 39 42 58 642.2 0 58 43 114 642.2 0 114 44 110 642.2 0 110 45 24 642.2 0 24 46 37 642.2 0 37 47 50 642.2 0 50 48 82 642.2 0 82 49 72 642.2 0 72 50 120 642.2 0 120 51 125 642.2 0 125 52 124 642.2 0 124 53 227 642.2 0 227 54 846 642.2 0 846 55 9738 642.2 0 9738 56 7625 642.2 0 7625 57 6393 642.2 0 6393 58 60 642.2 0 60 59 37 642.2 0 37 60 50 642.2 0 50 61 55 642.2 0 55 62 43 642.2 0 43 63 381 642.2 0 381 64 34 642.2 0 34 65 36 642.2 0 36 66 26 642.2 0 26 67 20 642.2 0 20 68 24 642.2 0 24 69 28 642.2 0 28 70 31 642.2 0 31 71 32 642.2 0 32 72 40 642.2 0 40 73 35 642.2 0 35 74 47 642.2 0 47 75 25 642.2 0 25 76 35 642.2 0 35 77 27 642.2 0 27 78 19 642.2 0 19 79 29 642.2 0 29 80 30 642.2 0 30 81 67 642.2 0 67 82 46 642.2 0 46 83 50 642.2 0 50 84 67 642.2 0 67 85 397 642.2 0 397 86 233 642.2 0 233 87 245 642.2 0 245 88 34 642.2 0 34 89 30 642.2 0 30 90 50 642.2 0 50 91 31 642.2 0 31 92 31 642.2 0 31 93 32 642.2 0 32 94 31 642.2 0 31 95 19 642.2 0 19 96 44 642.2 0 44 97 30 642.2 0 30 98 26 642.2 0 26 99 32 642.2 0 32 100 37 642.2 0 37 101 82 642.2 0 82 102 123 642.2 0 123 103 134 642.2 0 134 104 93 642.2 0 93 105 37 642.2 0 37 106 53 642.2 0 53 107 48 642.2 0 48 108 55 642.2 0 55 109 34 642.2 0 34 110 27 642.2 0 27 111 29 642.2 0 29 112 40 642.2 0 40 113 28 642.2 0 28 114 35 642.2 0 35 115 36 642.2 0 36 116 39 642.2 0 39 117 125 642.2 0 125 118 216 642.2 0 216 119 1328 642.2 0 1328 120 28 642.2 0 28 121 27 642.2 0 27 122 87 642.2 0 87 123 30 642.2 0 30 124 41 642.2 0 41 125 33 642.2 0 33 126 27 642.2 0 27 127 202 642.2 0 202 128 33 642.2 0 33 129 36 642.2 0 36 130 52 642.2 0 52 131 34 642.2 0 34 132 23 642.2 0 23 133 48 642.2 0 48 134 39 642.2 0 39 135 35 642.2 0 35 136 18 642.2 0 18 137 107 642.2 0 107 138 90 642.2 0 90 139 173 642.2 0 173 140 59 642.2 0 59 141 30 642.2 0 30 142 39 642.2 0 39 143 30 642.2 0 30 144 22 642.2 0 22 145 48 642.2 0 48 146 22 642.2 0 22 147 41 642.2 0 41 148 51 642.2 0 51 149 32 642.2 0 32 150 32 642.2 0 32 151 31 642.2 0 31 152 36 642.2 0 36 153 41 642.2 0 41 154 51 642.2 0 51 155 52 642.2 0 52 156 256 642.2 0 256 157 81 642.2 0 81 158 102 642.2 0 102 159 103 642.2 0 103 160 38 642.2 0 38 161 40 642.2 0 40 162 54 642.2 0 54 163 30 642.2 0 30 164 207 642.2 0 207 165 51 642.2 0 51 166 137 642.2 0 137 167 133 642.2 0 133 168 94 642.2 0 94 169 198 642.2 0 198 170 49 642.2 0 49 171 25 642.2 0 25 172 19 642.2 0 19 173 44 642.2 0 44 174 44 642.2 0 44 175 47 642.2 0 47 176 200 642.2 0 200 177 36 642.2 0 36 178 40 642.2 0 40 179 28 642.2 0 28 180 20 642.2 0 20 181 59 642.2 0 59 182 33 642.2 0 33 183 55 642.2 0 55 184 68 642.2 0 68 185 36 642.2 0 36 186 49 642.2 0 49 187 179 642.2 0 179 188 184 642.2 0 184 189 178 642.2 0 178 190 303 642.2 0 303 191 82 642.2 0 82 192 75 642.2 0 75 193 26 642.2 0 26 194 40 642.2 0 40 195 50 642.2 0 50 196 32 642.2 0 32 197 67 642.2 0 67 198 40 642.2 0 40 199 50 642.2 0 50 200 61 642.2 0 61 201 33 642.2 0 33 202 28 642.2 0 28 203 91 642.2 0 91 204 59 642.2 0 59 205 25 642.2 0 25 206 43 642.2 0 43 207 48 642.2 0 48 208 36 642.2 0 36 209 386 642.2 0 386 210 50 642.2 0 50 211 45 642.2 0 45 212 39 642.2 0 39 213 30 642.2 0 30 214 29 642.2 0 29 215 69 642.2 0 69 216 62 642.2 0 62 217 24 642.2 0 24 218 39 642.2 0 39 219 146 642.2 0 146 220 245 642.2 0 245 221 1677 642.2 0 1677 222 28 642.2 0 28 223 31 642.2 0 31 224 35 642.2 0 35 225 35 642.2 0 35 226 27 642.2 0 27 227 11 642.2 0 11 228 27 642.2 0 27 229 43 642.2 0 43 230 36 642.2 0 36 231 46 642.2 0 46 232 28 642.2 0 28 233 61 642.2 0 61 234 55 642.2 0 55 235 55 642.2 0 55 236 34 642.2 0 34 237 25 642.2 0 25 238 253 642.2 0 253 239 57 642.2 0 57 240 59 642.2 0 59 241 52 642.2 0 52 242 29 642.2 0 29 243 34 642.2 0 34 244 31 642.2 0 31 245 35 642.2 0 35 246 90 642.2 0 90 247 56 642.2 0 56 248 629 642.2 0 629 249 50 642.2 0 50 250 59 642.2 0 59 251 39 642.2 0 39 252 47 642.2 0 47 253 21 642.2 0 21 254 22 642.2 0 22 255 9 642.2 0 9 256 20 642.2 0 20 257 46 642.2 0 46 258 102 642.2 0 102 259 160 642.2 0 160 260 94 642.2 0 94 261 96 642.2 0 96 262 80 642.2 0 80 263 58 642.2 0 58 264 41 642.2 0 41 265 34 642.2 0 34 266 33 642.2 0 33 267 65 642.2 0 65 268 24 642.2 0 24 269 57 642.2 0 57 270 31 642.2 0 31 271 21 642.2 0 21 272 36 642.2 0 36 273 41 642.2 0 41 274 51 642.2 0 51 275 50 642.2 0 50 276 111 642.2 0 111 277 63 642.2 0 63 278 259 642.2 0 259 279 179 642.2 0 179 280 101 642.2 0 101 281 52 642.2 0 52 282 65 642.2 0 65 283 75 642.2 0 75 284 49 642.2 0 49 285 69 642.2 0 69 286 48 642.2 0 48 287 78 642.2 0 78 288 122 642.2 0 122 289 86 642.2 0 86 290 128 642.2 0 128 291 84 642.2 0 84 292 77 642.2 0 77 293 57 642.2 0 57 294 110 642.2 0 110 295 60 642.2 0 60 296 85 642.2 0 85 297 86 642.2 0 86 298 642 642.2 0 642 299 519 642.2 0 519 300 443 642.2 0 443 301 105 642.2 0 105 Finished in 1.92 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 44,334 Reads with adapters: 3,040 (6.9%) Reads that were too short: 29 (0.1%) Reads written (passing filters): 3,011 (6.8%) Total basepairs processed: 10,421,255 bp Total written (filtered): 825,403 bp (7.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3040 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 16 0.0 3 0 16 17 2817 0.0 3 2751 65 1 18 6 0.0 3 0 6 19 9 0.0 3 8 0 1 20 1 0.0 3 0 0 0 1 21 1 0.0 3 0 0 0 1 24 7 0.0 3 0 0 2 5 29 1 0.0 3 0 0 0 1 32 5 0.0 3 0 0 0 5 33 1 0.0 3 0 0 0 1 34 7 0.0 3 0 0 0 7 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 41 1 0.0 3 1 42 1 0.0 3 1 47 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 114 0.0 3 0 0 29 85 53 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 75 1 0.0 3 1 77 7 0.0 3 1 0 0 6 81 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 112 1 0.0 3 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 1 121 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 235 3 0.0 3 0 0 0 3 245 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 3,011 Reads with adapters: 13 (0.4%) Reads written (passing filters): 3,011 (100.0%) Total basepairs processed: 825,403 bp Total written (filtered): 825,357 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 13 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.5% C: 15.4% G: 15.4% T: 7.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 9 47.0 0 9 4 2 11.8 0 2 5 1 2.9 0 1 6 1 0.7 0 1