This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.89 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 466,194 (19.1%) Reads that were too short: 374,090 (15.3%) Reads written (passing filters): 92,104 (3.8%) Total basepairs processed: 735,190,694 bp Total written (filtered): 15,653,518 bp (2.1%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 466194 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1122 596.3 0 1122 7 10044 596.3 0 10044 8 248 596.3 0 248 9 9281 596.3 0 9281 10 146 596.3 0 146 11 2916 596.3 0 2916 12 34 596.3 0 34 13 34 596.3 0 34 14 22 596.3 0 22 15 15 596.3 0 15 16 19 596.3 0 19 17 33 596.3 0 33 18 29 596.3 0 29 19 33 596.3 0 33 20 26 596.3 0 26 21 36 596.3 0 36 22 28 596.3 0 28 23 17 596.3 0 17 24 20 596.3 0 20 25 25 596.3 0 25 26 19 596.3 0 19 27 18 596.3 0 18 28 21 596.3 0 21 29 29 596.3 0 29 30 19 596.3 0 19 31 77 596.3 0 77 32 79 596.3 0 79 33 33 596.3 0 33 34 57 596.3 0 57 35 73 596.3 0 73 36 30 596.3 0 30 37 34 596.3 0 34 38 86 596.3 0 86 39 30 596.3 0 30 40 26 596.3 0 26 41 36 596.3 0 36 42 25 596.3 0 25 43 29 596.3 0 29 44 33 596.3 0 33 45 31 596.3 0 31 46 20 596.3 0 20 47 24 596.3 0 24 48 20 596.3 0 20 49 23 596.3 0 23 50 25 596.3 0 25 51 16 596.3 0 16 52 27 596.3 0 27 53 29 596.3 0 29 54 37 596.3 0 37 55 21 596.3 0 21 56 23 596.3 0 23 57 45 596.3 0 45 58 34 596.3 0 34 59 62 596.3 0 62 60 51 596.3 0 51 61 35 596.3 0 35 62 16 596.3 0 16 63 20 596.3 0 20 64 22 596.3 0 22 65 72 596.3 0 72 66 28 596.3 0 28 67 55 596.3 0 55 68 18 596.3 0 18 69 42 596.3 0 42 70 36 596.3 0 36 71 101 596.3 0 101 72 33 596.3 0 33 73 19 596.3 0 19 74 34 596.3 0 34 75 113 596.3 0 113 76 85 596.3 0 85 77 142 596.3 0 142 78 29 596.3 0 29 79 30 596.3 0 30 80 23 596.3 0 23 81 30 596.3 0 30 82 31 596.3 0 31 83 15 596.3 0 15 84 35 596.3 0 35 85 26 596.3 0 26 86 31 596.3 0 31 87 25 596.3 0 25 88 32 596.3 0 32 89 38 596.3 0 38 90 31 596.3 0 31 91 45 596.3 0 45 92 42 596.3 0 42 93 26 596.3 0 26 94 33 596.3 0 33 95 47 596.3 0 47 96 31 596.3 0 31 97 25 596.3 0 25 98 32 596.3 0 32 99 45 596.3 0 45 100 69 596.3 0 69 101 36 596.3 0 36 102 41 596.3 0 41 103 58 596.3 0 58 104 32 596.3 0 32 105 30 596.3 0 30 106 60 596.3 0 60 107 51 596.3 0 51 108 47 596.3 0 47 109 41 596.3 0 41 110 54 596.3 0 54 111 74 596.3 0 74 112 74 596.3 0 74 113 1334 596.3 0 1334 114 2426 596.3 0 2426 115 1584 596.3 0 1584 116 64 596.3 0 64 117 32 596.3 0 32 118 45 596.3 0 45 119 32 596.3 0 32 120 41 596.3 0 41 121 87 596.3 0 87 122 78 596.3 0 78 123 66 596.3 0 66 124 59 596.3 0 59 125 49 596.3 0 49 126 45 596.3 0 45 127 85 596.3 0 85 128 615 596.3 0 615 129 137 596.3 0 137 130 509 596.3 0 509 131 33 596.3 0 33 132 44 596.3 0 44 133 43 596.3 0 43 134 84 596.3 0 84 135 67 596.3 0 67 136 54 596.3 0 54 137 83 596.3 0 83 138 49 596.3 0 49 139 66 596.3 0 66 140 55 596.3 0 55 141 77 596.3 0 77 142 103 596.3 0 103 143 293 596.3 0 293 144 418 596.3 0 418 145 133 596.3 0 133 146 102 596.3 0 102 147 2100 596.3 0 2100 148 2089 596.3 0 2089 149 3123 596.3 0 3123 150 152 596.3 0 152 151 59 596.3 0 59 152 40 596.3 0 40 153 28 596.3 0 28 154 53 596.3 0 53 155 63 596.3 0 63 156 63 596.3 0 63 157 66 596.3 0 66 158 62 596.3 0 62 159 42 596.3 0 42 160 51 596.3 0 51 161 80 596.3 0 80 162 88 596.3 0 88 163 70 596.3 0 70 164 55 596.3 0 55 165 63 596.3 0 63 166 44 596.3 0 44 167 48 596.3 0 48 168 109 596.3 0 109 169 121 596.3 0 121 170 597 596.3 0 597 171 40 596.3 0 40 172 59 596.3 0 59 173 74 596.3 0 74 174 198 596.3 0 198 175 58 596.3 0 58 176 94 596.3 0 94 177 77 596.3 0 77 178 82 596.3 0 82 179 159 596.3 0 159 180 125 596.3 0 125 181 215 596.3 0 215 182 185 596.3 0 185 183 141 596.3 0 141 184 141 596.3 0 141 185 186 596.3 0 186 186 255 596.3 0 255 187 414 596.3 0 414 188 314 596.3 0 314 189 1543 596.3 0 1543 190 1738 596.3 0 1738 191 2980 596.3 0 2980 192 1154 596.3 0 1154 193 757 596.3 0 757 194 561 596.3 0 561 195 981 596.3 0 981 196 753 596.3 0 753 197 704 596.3 0 704 198 858 596.3 0 858 199 2350 596.3 0 2350 200 10914 596.3 0 10914 201 18126 596.3 0 18126 202 15932 596.3 0 15932 203 1885 596.3 0 1885 204 1738 596.3 0 1738 205 18574 596.3 0 18574 206 42872 596.3 0 42872 207 55223 596.3 0 55223 208 24768 596.3 0 24768 209 7009 596.3 0 7009 210 3581 596.3 0 3581 211 2894 596.3 0 2894 212 2010 596.3 0 2010 213 1628 596.3 0 1628 214 598 596.3 0 598 215 1315 596.3 0 1315 216 2125 596.3 0 2125 217 1061 596.3 0 1061 218 1790 596.3 0 1790 219 4051 596.3 0 4051 220 2731 596.3 0 2731 221 3561 596.3 0 3561 222 3584 596.3 0 3584 223 1817 596.3 0 1817 224 2073 596.3 0 2073 225 10540 596.3 0 10540 226 41003 596.3 0 41003 227 31887 596.3 0 31887 228 16828 596.3 0 16828 229 85 596.3 0 85 230 22 596.3 0 22 231 37 596.3 0 37 232 40 596.3 0 40 233 23 596.3 0 23 234 28 596.3 0 28 235 45 596.3 0 45 236 80 596.3 0 80 237 41 596.3 0 41 238 144 596.3 0 144 239 54 596.3 0 54 240 64 596.3 0 64 241 59 596.3 0 59 242 55 596.3 0 55 243 143 596.3 0 143 244 596 596.3 0 596 245 468 596.3 0 468 246 503 596.3 0 503 247 188 596.3 0 188 248 339 596.3 0 339 249 636 596.3 0 636 250 352 596.3 0 352 251 332 596.3 0 332 252 872 596.3 0 872 253 243 596.3 0 243 254 644 596.3 0 644 255 2028 596.3 0 2028 256 662 596.3 0 662 257 386 596.3 0 386 258 340 596.3 0 340 259 578 596.3 0 578 260 785 596.3 0 785 261 895 596.3 0 895 262 12503 596.3 0 12503 263 24211 596.3 0 24211 264 19153 596.3 0 19153 265 292 596.3 0 292 266 105 596.3 0 105 267 20 596.3 0 20 268 36 596.3 0 36 269 55 596.3 0 55 270 100 596.3 0 100 271 275 596.3 0 275 272 121 596.3 0 121 273 21 596.3 0 21 274 63 596.3 0 63 275 126 596.3 0 126 276 41 596.3 0 41 277 28 596.3 0 28 278 40 596.3 0 40 279 27 596.3 0 27 280 20 596.3 0 20 281 28 596.3 0 28 282 56 596.3 0 56 283 108 596.3 0 108 284 79 596.3 0 79 285 49 596.3 0 49 286 24 596.3 0 24 287 26 596.3 0 26 288 29 596.3 0 29 289 27 596.3 0 27 290 36 596.3 0 36 291 32 596.3 0 32 292 163 596.3 0 163 293 273 596.3 0 273 294 377 596.3 0 377 295 324 596.3 0 324 296 97 596.3 0 97 297 90 596.3 0 90 298 70 596.3 0 70 299 46 596.3 0 46 300 29 596.3 0 29 301 45 596.3 0 45 Finished in 3.21 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 92,104 Reads with adapters: 9,680 (10.5%) Reads that were too short: 121 (0.1%) Reads written (passing filters): 9,559 (10.4%) Total basepairs processed: 15,653,518 bp Total written (filtered): 2,617,851 bp (16.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9680 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 101 0.0 3 0 97 4 17 9339 0.0 3 9126 203 9 1 18 29 0.0 3 0 23 1 5 19 12 0.0 3 1 0 10 1 20 5 0.0 3 0 0 0 5 26 42 0.0 3 0 0 0 42 33 9 0.0 3 0 0 0 9 39 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 7 0.0 3 0 0 0 7 49 2 0.0 3 0 0 0 2 51 3 0.0 3 0 0 0 3 56 3 0.0 3 0 0 0 3 58 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 65 4 0.0 3 0 0 0 4 69 2 0.0 3 0 0 0 2 75 25 0.0 3 0 0 0 25 76 19 0.0 3 0 0 0 19 78 28 0.0 3 0 0 0 28 79 1 0.0 3 0 0 0 1 81 8 0.0 3 0 0 0 8 90 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 106 9 0.0 3 0 0 0 9 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 170 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 177 1 0.0 3 1 178 1 0.0 3 0 0 0 1 180 3 0.0 3 0 0 0 3 222 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 9,559 Reads with adapters: 157 (1.6%) Reads written (passing filters): 9,559 (100.0%) Total basepairs processed: 2,617,851 bp Total written (filtered): 2,617,376 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 157 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 68.2% C: 12.1% G: 18.5% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 153 149.4 0 153 4 4 37.3 0 4