This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.03 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 109,607 (4.5%) Reads that were too short: 47,961 (2.0%) Reads written (passing filters): 61,646 (2.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 12,483,161 bp (1.7%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 109607 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 303 596.3 0 303 7 846 596.3 0 846 8 9194 596.3 0 9194 9 7836 596.3 0 7836 10 23 596.3 0 23 11 15 596.3 0 15 12 26 596.3 0 26 13 22 596.3 0 22 14 18 596.3 0 18 15 17 596.3 0 17 16 6 596.3 0 6 17 31 596.3 0 31 18 12 596.3 0 12 19 17 596.3 0 17 20 13 596.3 0 13 21 15 596.3 0 15 22 19 596.3 0 19 23 19 596.3 0 19 24 18 596.3 0 18 25 31 596.3 0 31 26 18 596.3 0 18 27 25 596.3 0 25 28 15 596.3 0 15 29 20 596.3 0 20 30 12 596.3 0 12 31 18 596.3 0 18 32 178 596.3 0 178 33 31 596.3 0 31 34 53 596.3 0 53 35 38 596.3 0 38 36 36 596.3 0 36 37 238 596.3 0 238 38 66 596.3 0 66 39 191 596.3 0 191 40 36 596.3 0 36 41 35 596.3 0 35 42 124 596.3 0 124 43 18 596.3 0 18 44 27 596.3 0 27 45 15 596.3 0 15 46 24 596.3 0 24 47 28 596.3 0 28 48 37 596.3 0 37 49 34 596.3 0 34 50 15 596.3 0 15 51 21 596.3 0 21 52 425 596.3 0 425 53 155 596.3 0 155 54 425 596.3 0 425 55 22 596.3 0 22 56 18 596.3 0 18 57 31 596.3 0 31 58 30 596.3 0 30 59 24 596.3 0 24 60 28 596.3 0 28 61 45 596.3 0 45 62 37 596.3 0 37 63 22 596.3 0 22 64 15 596.3 0 15 65 23 596.3 0 23 66 69 596.3 0 69 67 29 596.3 0 29 68 111 596.3 0 111 69 16 596.3 0 16 70 19 596.3 0 19 71 51 596.3 0 51 72 75 596.3 0 75 73 106 596.3 0 106 74 16 596.3 0 16 75 10 596.3 0 10 76 8 596.3 0 8 77 15 596.3 0 15 78 8 596.3 0 8 79 12 596.3 0 12 80 16 596.3 0 16 81 25 596.3 0 25 82 21 596.3 0 21 83 35 596.3 0 35 84 123 596.3 0 123 85 130 596.3 0 130 86 217 596.3 0 217 87 28 596.3 0 28 88 22 596.3 0 22 89 27 596.3 0 27 90 122 596.3 0 122 91 84 596.3 0 84 92 115 596.3 0 115 93 97 596.3 0 97 94 101 596.3 0 101 95 124 596.3 0 124 96 27 596.3 0 27 97 47 596.3 0 47 98 11 596.3 0 11 99 19 596.3 0 19 100 21 596.3 0 21 101 14 596.3 0 14 102 17 596.3 0 17 103 25 596.3 0 25 104 72 596.3 0 72 105 75 596.3 0 75 106 68 596.3 0 68 107 95 596.3 0 95 108 181 596.3 0 181 109 189 596.3 0 189 110 259 596.3 0 259 111 142 596.3 0 142 112 301 596.3 0 301 113 127 596.3 0 127 114 22 596.3 0 22 115 33 596.3 0 33 116 34 596.3 0 34 117 105 596.3 0 105 118 54 596.3 0 54 119 66 596.3 0 66 120 17 596.3 0 17 121 13 596.3 0 13 122 14 596.3 0 14 123 26 596.3 0 26 124 33 596.3 0 33 125 53 596.3 0 53 126 77 596.3 0 77 127 390 596.3 0 390 128 120 596.3 0 120 129 365 596.3 0 365 130 55 596.3 0 55 131 69 596.3 0 69 132 174 596.3 0 174 133 58 596.3 0 58 134 84 596.3 0 84 135 21 596.3 0 21 136 37 596.3 0 37 137 51 596.3 0 51 138 77 596.3 0 77 139 130 596.3 0 130 140 228 596.3 0 228 141 263 596.3 0 263 142 347 596.3 0 347 143 768 596.3 0 768 144 11387 596.3 0 11387 145 7622 596.3 0 7622 146 9034 596.3 0 9034 147 110 596.3 0 110 148 84 596.3 0 84 149 32 596.3 0 32 150 43 596.3 0 43 151 249 596.3 0 249 152 193 596.3 0 193 153 297 596.3 0 297 154 130 596.3 0 130 155 90 596.3 0 90 156 196 596.3 0 196 157 311 596.3 0 311 158 208 596.3 0 208 159 84 596.3 0 84 160 52 596.3 0 52 161 69 596.3 0 69 162 27 596.3 0 27 163 25 596.3 0 25 164 22 596.3 0 22 165 42 596.3 0 42 166 42 596.3 0 42 167 25 596.3 0 25 168 28 596.3 0 28 169 35 596.3 0 35 170 56 596.3 0 56 171 82 596.3 0 82 172 132 596.3 0 132 173 70 596.3 0 70 174 56 596.3 0 56 175 38 596.3 0 38 176 40 596.3 0 40 177 52 596.3 0 52 178 65 596.3 0 65 179 310 596.3 0 310 180 101 596.3 0 101 181 249 596.3 0 249 182 37 596.3 0 37 183 23 596.3 0 23 184 43 596.3 0 43 185 48 596.3 0 48 186 59 596.3 0 59 187 37 596.3 0 37 188 38 596.3 0 38 189 43 596.3 0 43 190 218 596.3 0 218 191 84 596.3 0 84 192 280 596.3 0 280 193 48 596.3 0 48 194 35 596.3 0 35 195 29 596.3 0 29 196 40 596.3 0 40 197 71 596.3 0 71 198 150 596.3 0 150 199 90 596.3 0 90 200 79 596.3 0 79 201 66 596.3 0 66 202 27 596.3 0 27 203 49 596.3 0 49 204 33 596.3 0 33 205 46 596.3 0 46 206 58 596.3 0 58 207 70 596.3 0 70 208 27 596.3 0 27 209 42 596.3 0 42 210 26 596.3 0 26 211 18 596.3 0 18 212 43 596.3 0 43 213 36 596.3 0 36 214 32 596.3 0 32 215 21 596.3 0 21 216 30 596.3 0 30 217 103 596.3 0 103 218 81 596.3 0 81 219 508 596.3 0 508 220 30 596.3 0 30 221 22 596.3 0 22 222 32 596.3 0 32 223 28 596.3 0 28 224 34 596.3 0 34 225 76 596.3 0 76 226 49 596.3 0 49 227 89 596.3 0 89 228 98 596.3 0 98 229 78 596.3 0 78 230 196 596.3 0 196 231 140 596.3 0 140 232 166 596.3 0 166 233 88 596.3 0 88 234 86 596.3 0 86 235 281 596.3 0 281 236 595 596.3 0 595 237 562 596.3 0 562 238 731 596.3 0 731 239 154 596.3 0 154 240 808 596.3 0 808 241 299 596.3 0 299 242 813 596.3 0 813 243 97 596.3 0 97 244 70 596.3 0 70 245 143 596.3 0 143 246 147 596.3 0 147 247 220 596.3 0 220 248 178 596.3 0 178 249 160 596.3 0 160 250 335 596.3 0 335 251 561 596.3 0 561 252 595 596.3 0 595 253 154 596.3 0 154 254 73 596.3 0 73 255 101 596.3 0 101 256 158 596.3 0 158 257 147 596.3 0 147 258 237 596.3 0 237 259 539 596.3 0 539 260 6680 596.3 0 6680 261 4013 596.3 0 4013 262 6203 596.3 0 6203 263 1532 596.3 0 1532 264 70 596.3 0 70 265 64 596.3 0 64 266 101 596.3 0 101 267 180 596.3 0 180 268 184 596.3 0 184 269 140 596.3 0 140 270 280 596.3 0 280 271 461 596.3 0 461 272 357 596.3 0 357 273 125 596.3 0 125 274 72 596.3 0 72 275 54 596.3 0 54 276 122 596.3 0 122 277 191 596.3 0 191 278 171 596.3 0 171 279 247 596.3 0 247 280 340 596.3 0 340 281 445 596.3 0 445 282 463 596.3 0 463 283 2086 596.3 0 2086 284 6386 596.3 0 6386 285 1421 596.3 0 1421 286 101 596.3 0 101 287 141 596.3 0 141 288 350 596.3 0 350 289 256 596.3 0 256 290 255 596.3 0 255 291 407 596.3 0 407 292 510 596.3 0 510 293 908 596.3 0 908 294 635 596.3 0 635 295 497 596.3 0 497 296 537 596.3 0 537 297 132 596.3 0 132 298 49 596.3 0 49 299 57 596.3 0 57 300 53 596.3 0 53 301 65 596.3 0 65 Finished in 2.65 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 61,646 Reads with adapters: 8,370 (13.6%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 8,357 (13.6%) Total basepairs processed: 12,483,161 bp Total written (filtered): 2,265,943 bp (18.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8370 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 4 0.0 3 0 0 2 2 19 129 0.0 3 0 118 7 2 2 20 7996 0.0 4 7596 328 50 17 5 21 18 0.0 4 3 11 3 0 1 22 3 0.0 4 0 1 1 1 23 132 0.0 4 98 7 0 25 2 24 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 33 4 0.0 4 0 0 0 0 4 58 1 0.0 4 0 0 0 1 63 2 0.0 4 0 0 0 0 2 65 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 75 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 0 2 127 60 0.0 4 0 0 0 0 60 130 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 234 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 8,357 Reads with adapters: 1,900 (22.7%) Reads written (passing filters): 8,357 (100.0%) Total basepairs processed: 2,265,943 bp Total written (filtered): 2,245,921 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1900 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 10.4% G: 85.3% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 130.6 0 4 4 1 32.6 0 1 8 5 0.1 0 5 9 204 0.0 0 14 190 10 1411 0.0 1 109 1302 11 263 0.0 1 6 257 12 7 0.0 1 0 7 150 1 0.0 1 1 223 3 0.0 1 2 1 224 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.