This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.85 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 102,812 (4.2%) Reads that were too short: 38,650 (1.6%) Reads written (passing filters): 64,162 (2.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 12,896,204 bp (1.8%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 102812 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 273 596.3 0 273 7 594 596.3 0 594 8 8084 596.3 0 8084 9 10194 596.3 0 10194 10 15 596.3 0 15 11 11 596.3 0 11 12 6 596.3 0 6 13 7 596.3 0 7 14 8 596.3 0 8 15 11 596.3 0 11 16 4 596.3 0 4 17 13 596.3 0 13 18 4 596.3 0 4 19 7 596.3 0 7 20 4 596.3 0 4 21 4 596.3 0 4 22 15 596.3 0 15 23 10 596.3 0 10 24 12 596.3 0 12 25 8 596.3 0 8 26 6 596.3 0 6 27 10 596.3 0 10 28 6 596.3 0 6 29 9 596.3 0 9 30 3 596.3 0 3 31 11 596.3 0 11 32 145 596.3 0 145 33 29 596.3 0 29 34 38 596.3 0 38 35 24 596.3 0 24 36 24 596.3 0 24 37 144 596.3 0 144 38 101 596.3 0 101 39 201 596.3 0 201 40 28 596.3 0 28 41 19 596.3 0 19 42 17 596.3 0 17 43 10 596.3 0 10 44 11 596.3 0 11 45 10 596.3 0 10 46 14 596.3 0 14 47 13 596.3 0 13 48 18 596.3 0 18 49 13 596.3 0 13 50 14 596.3 0 14 51 12 596.3 0 12 52 158 596.3 0 158 53 79 596.3 0 79 54 215 596.3 0 215 55 14 596.3 0 14 56 12 596.3 0 12 57 6 596.3 0 6 58 12 596.3 0 12 59 11 596.3 0 11 60 23 596.3 0 23 61 24 596.3 0 24 62 724 596.3 0 724 63 103 596.3 0 103 64 15 596.3 0 15 65 9 596.3 0 9 66 17 596.3 0 17 67 13 596.3 0 13 68 13 596.3 0 13 69 6 596.3 0 6 70 12 596.3 0 12 71 22 596.3 0 22 72 42 596.3 0 42 73 42 596.3 0 42 74 9 596.3 0 9 75 8 596.3 0 8 76 8 596.3 0 8 77 11 596.3 0 11 78 8 596.3 0 8 79 6 596.3 0 6 80 9 596.3 0 9 81 15 596.3 0 15 82 16 596.3 0 16 83 14 596.3 0 14 84 65 596.3 0 65 85 66 596.3 0 66 86 104 596.3 0 104 87 14 596.3 0 14 88 14 596.3 0 14 89 16 596.3 0 16 90 41 596.3 0 41 91 33 596.3 0 33 92 47 596.3 0 47 93 25 596.3 0 25 94 26 596.3 0 26 95 65 596.3 0 65 96 19 596.3 0 19 97 44 596.3 0 44 98 11 596.3 0 11 99 12 596.3 0 12 100 21 596.3 0 21 101 11 596.3 0 11 102 7 596.3 0 7 103 18 596.3 0 18 104 47 596.3 0 47 105 43 596.3 0 43 106 37 596.3 0 37 107 44 596.3 0 44 108 67 596.3 0 67 109 68 596.3 0 68 110 83 596.3 0 83 111 61 596.3 0 61 112 94 596.3 0 94 113 45 596.3 0 45 114 32 596.3 0 32 115 30 596.3 0 30 116 14 596.3 0 14 117 102 596.3 0 102 118 29 596.3 0 29 119 58 596.3 0 58 120 45 596.3 0 45 121 10 596.3 0 10 122 8 596.3 0 8 123 14 596.3 0 14 124 30 596.3 0 30 125 24 596.3 0 24 126 54 596.3 0 54 127 329 596.3 0 329 128 102 596.3 0 102 129 259 596.3 0 259 130 46 596.3 0 46 131 59 596.3 0 59 132 161 596.3 0 161 133 57 596.3 0 57 134 67 596.3 0 67 135 30 596.3 0 30 136 23 596.3 0 23 137 53 596.3 0 53 138 55 596.3 0 55 139 125 596.3 0 125 140 212 596.3 0 212 141 241 596.3 0 241 142 381 596.3 0 381 143 882 596.3 0 882 144 14209 596.3 0 14209 145 9213 596.3 0 9213 146 11379 596.3 0 11379 147 100 596.3 0 100 148 90 596.3 0 90 149 21 596.3 0 21 150 10 596.3 0 10 151 81 596.3 0 81 152 53 596.3 0 53 153 89 596.3 0 89 154 47 596.3 0 47 155 53 596.3 0 53 156 60 596.3 0 60 157 141 596.3 0 141 158 115 596.3 0 115 159 25 596.3 0 25 160 27 596.3 0 27 161 21 596.3 0 21 162 14 596.3 0 14 163 10 596.3 0 10 164 7 596.3 0 7 165 17 596.3 0 17 166 16 596.3 0 16 167 12 596.3 0 12 168 8 596.3 0 8 169 21 596.3 0 21 170 25 596.3 0 25 171 25 596.3 0 25 172 49 596.3 0 49 173 19 596.3 0 19 174 14 596.3 0 14 175 25 596.3 0 25 176 11 596.3 0 11 177 29 596.3 0 29 178 38 596.3 0 38 179 193 596.3 0 193 180 38 596.3 0 38 181 100 596.3 0 100 182 19 596.3 0 19 183 17 596.3 0 17 184 16 596.3 0 16 185 21 596.3 0 21 186 24 596.3 0 24 187 13 596.3 0 13 188 17 596.3 0 17 189 28 596.3 0 28 190 280 596.3 0 280 191 111 596.3 0 111 192 263 596.3 0 263 193 25 596.3 0 25 194 20 596.3 0 20 195 15 596.3 0 15 196 13 596.3 0 13 197 34 596.3 0 34 198 66 596.3 0 66 199 53 596.3 0 53 200 33 596.3 0 33 201 19 596.3 0 19 202 12 596.3 0 12 203 20 596.3 0 20 204 16 596.3 0 16 205 19 596.3 0 19 206 17 596.3 0 17 207 21 596.3 0 21 208 14 596.3 0 14 209 18 596.3 0 18 210 11 596.3 0 11 211 10 596.3 0 10 212 15 596.3 0 15 213 25 596.3 0 25 214 19 596.3 0 19 215 23 596.3 0 23 216 21 596.3 0 21 217 139 596.3 0 139 218 61 596.3 0 61 219 838 596.3 0 838 220 22 596.3 0 22 221 16 596.3 0 16 222 15 596.3 0 15 223 19 596.3 0 19 224 10 596.3 0 10 225 36 596.3 0 36 226 26 596.3 0 26 227 38 596.3 0 38 228 37 596.3 0 37 229 51 596.3 0 51 230 128 596.3 0 128 231 101 596.3 0 101 232 146 596.3 0 146 233 29 596.3 0 29 234 35 596.3 0 35 235 123 596.3 0 123 236 273 596.3 0 273 237 245 596.3 0 245 238 271 596.3 0 271 239 56 596.3 0 56 240 462 596.3 0 462 241 183 596.3 0 183 242 422 596.3 0 422 243 32 596.3 0 32 244 27 596.3 0 27 245 41 596.3 0 41 246 45 596.3 0 45 247 104 596.3 0 104 248 79 596.3 0 79 249 67 596.3 0 67 250 102 596.3 0 102 251 236 596.3 0 236 252 255 596.3 0 255 253 42 596.3 0 42 254 29 596.3 0 29 255 33 596.3 0 33 256 49 596.3 0 49 257 65 596.3 0 65 258 128 596.3 0 128 259 267 596.3 0 267 260 3192 596.3 0 3192 261 1793 596.3 0 1793 262 2541 596.3 0 2541 263 807 596.3 0 807 264 29 596.3 0 29 265 32 596.3 0 32 266 59 596.3 0 59 267 78 596.3 0 78 268 92 596.3 0 92 269 96 596.3 0 96 270 220 596.3 0 220 271 429 596.3 0 429 272 235 596.3 0 235 273 141 596.3 0 141 274 74 596.3 0 74 275 58 596.3 0 58 276 149 596.3 0 149 277 161 596.3 0 161 278 164 596.3 0 164 279 237 596.3 0 237 280 265 596.3 0 265 281 361 596.3 0 361 282 380 596.3 0 380 283 3748 596.3 0 3748 284 13178 596.3 0 13178 285 2878 596.3 0 2878 286 37 596.3 0 37 287 58 596.3 0 58 288 182 596.3 0 182 289 133 596.3 0 133 290 99 596.3 0 99 291 153 596.3 0 153 292 127 596.3 0 127 293 226 596.3 0 226 294 148 596.3 0 148 295 121 596.3 0 121 296 149 596.3 0 149 297 35 596.3 0 35 298 24 596.3 0 24 299 30 596.3 0 30 300 28 596.3 0 28 301 59 596.3 0 59 Finished in 2.48 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 64,162 Reads with adapters: 8,346 (13.0%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 8,337 (13.0%) Total basepairs processed: 12,896,204 bp Total written (filtered): 2,299,563 bp (17.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8346 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 2 0.0 3 0 0 2 16 66 0.0 3 0 65 1 17 8218 0.0 3 8052 159 6 1 18 18 0.0 3 1 17 19 8 0.0 3 0 0 6 2 20 10 0.0 3 0 0 0 10 24 1 0.0 3 0 1 25 1 0.0 3 1 27 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 97 3 0.0 3 0 0 0 3 120 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.29 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 8,337 Reads with adapters: 64 (0.8%) Reads written (passing filters): 8,337 (100.0%) Total basepairs processed: 2,299,563 bp Total written (filtered): 2,299,365 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 64 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.5% C: 75.0% G: 9.4% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 61 130.3 0 61 5 3 8.1 0 3