This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 62.85 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 208,317 (8.5%) Reads that were too short: 6,681 (0.3%) Reads written (passing filters): 201,636 (8.3%) Total basepairs processed: 735,190,694 bp Total written (filtered): 57,471,695 bp (7.8%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 208317 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1240 596.3 0 1240 7 85989 596.3 0 85989 8 96544 596.3 0 96544 9 79 596.3 0 79 10 35 596.3 0 35 11 25 596.3 0 25 12 106 596.3 0 106 13 15 596.3 0 15 14 26 596.3 0 26 15 21 596.3 0 21 16 13 596.3 0 13 17 28 596.3 0 28 18 39 596.3 0 39 19 28 596.3 0 28 20 56 596.3 0 56 21 22 596.3 0 22 22 25 596.3 0 25 23 23 596.3 0 23 24 31 596.3 0 31 25 20 596.3 0 20 26 21 596.3 0 21 27 21 596.3 0 21 28 20 596.3 0 20 29 20 596.3 0 20 30 34 596.3 0 34 31 21 596.3 0 21 32 33 596.3 0 33 33 25 596.3 0 25 34 34 596.3 0 34 35 29 596.3 0 29 36 33 596.3 0 33 37 23 596.3 0 23 38 22 596.3 0 22 39 29 596.3 0 29 40 21 596.3 0 21 41 38 596.3 0 38 42 25 596.3 0 25 43 23 596.3 0 23 44 35 596.3 0 35 45 30 596.3 0 30 46 25 596.3 0 25 47 34 596.3 0 34 48 33 596.3 0 33 49 25 596.3 0 25 50 29 596.3 0 29 51 23 596.3 0 23 52 20 596.3 0 20 53 23 596.3 0 23 54 38 596.3 0 38 55 31 596.3 0 31 56 32 596.3 0 32 57 16 596.3 0 16 58 34 596.3 0 34 59 41 596.3 0 41 60 26 596.3 0 26 61 37 596.3 0 37 62 18 596.3 0 18 63 23 596.3 0 23 64 42 596.3 0 42 65 48 596.3 0 48 66 39 596.3 0 39 67 64 596.3 0 64 68 61 596.3 0 61 69 100 596.3 0 100 70 172 596.3 0 172 71 2885 596.3 0 2885 72 919 596.3 0 919 73 2503 596.3 0 2503 74 32 596.3 0 32 75 26 596.3 0 26 76 29 596.3 0 29 77 126 596.3 0 126 78 60 596.3 0 60 79 164 596.3 0 164 80 27 596.3 0 27 81 22 596.3 0 22 82 28 596.3 0 28 83 23 596.3 0 23 84 33 596.3 0 33 85 29 596.3 0 29 86 37 596.3 0 37 87 47 596.3 0 47 88 30 596.3 0 30 89 35 596.3 0 35 90 41 596.3 0 41 91 31 596.3 0 31 92 40 596.3 0 40 93 50 596.3 0 50 94 63 596.3 0 63 95 89 596.3 0 89 96 892 596.3 0 892 97 351 596.3 0 351 98 731 596.3 0 731 99 310 596.3 0 310 100 23 596.3 0 23 101 21 596.3 0 21 102 36 596.3 0 36 103 64 596.3 0 64 104 32 596.3 0 32 105 14 596.3 0 14 106 25 596.3 0 25 107 27 596.3 0 27 108 44 596.3 0 44 109 36 596.3 0 36 110 19 596.3 0 19 111 26 596.3 0 26 112 34 596.3 0 34 113 37 596.3 0 37 114 29 596.3 0 29 115 30 596.3 0 30 116 31 596.3 0 31 117 44 596.3 0 44 118 70 596.3 0 70 119 49 596.3 0 49 120 34 596.3 0 34 121 39 596.3 0 39 122 31 596.3 0 31 123 29 596.3 0 29 124 51 596.3 0 51 125 67 596.3 0 67 126 96 596.3 0 96 127 57 596.3 0 57 128 32 596.3 0 32 129 58 596.3 0 58 130 65 596.3 0 65 131 30 596.3 0 30 132 154 596.3 0 154 133 25 596.3 0 25 134 46 596.3 0 46 135 81 596.3 0 81 136 50 596.3 0 50 137 55 596.3 0 55 138 36 596.3 0 36 139 39 596.3 0 39 140 40 596.3 0 40 141 45 596.3 0 45 142 43 596.3 0 43 143 50 596.3 0 50 144 36 596.3 0 36 145 96 596.3 0 96 146 39 596.3 0 39 147 36 596.3 0 36 148 35 596.3 0 35 149 39 596.3 0 39 150 40 596.3 0 40 151 48 596.3 0 48 152 31 596.3 0 31 153 32 596.3 0 32 154 37 596.3 0 37 155 33 596.3 0 33 156 41 596.3 0 41 157 36 596.3 0 36 158 40 596.3 0 40 159 22 596.3 0 22 160 32 596.3 0 32 161 40 596.3 0 40 162 30 596.3 0 30 163 29 596.3 0 29 164 33 596.3 0 33 165 33 596.3 0 33 166 30 596.3 0 30 167 36 596.3 0 36 168 27 596.3 0 27 169 40 596.3 0 40 170 25 596.3 0 25 171 40 596.3 0 40 172 45 596.3 0 45 173 126 596.3 0 126 174 107 596.3 0 107 175 154 596.3 0 154 176 29 596.3 0 29 177 35 596.3 0 35 178 45 596.3 0 45 179 30 596.3 0 30 180 41 596.3 0 41 181 33 596.3 0 33 182 48 596.3 0 48 183 39 596.3 0 39 184 26 596.3 0 26 185 39 596.3 0 39 186 51 596.3 0 51 187 150 596.3 0 150 188 30 596.3 0 30 189 32 596.3 0 32 190 53 596.3 0 53 191 83 596.3 0 83 192 632 596.3 0 632 193 314 596.3 0 314 194 533 596.3 0 533 195 192 596.3 0 192 196 22 596.3 0 22 197 29 596.3 0 29 198 27 596.3 0 27 199 48 596.3 0 48 200 47 596.3 0 47 201 27 596.3 0 27 202 26 596.3 0 26 203 36 596.3 0 36 204 33 596.3 0 33 205 61 596.3 0 61 206 76 596.3 0 76 207 84 596.3 0 84 208 43 596.3 0 43 209 29 596.3 0 29 210 25 596.3 0 25 211 61 596.3 0 61 212 44 596.3 0 44 213 44 596.3 0 44 214 27 596.3 0 27 215 38 596.3 0 38 216 32 596.3 0 32 217 68 596.3 0 68 218 38 596.3 0 38 219 41 596.3 0 41 220 38 596.3 0 38 221 40 596.3 0 40 222 54 596.3 0 54 223 36 596.3 0 36 224 22 596.3 0 22 225 132 596.3 0 132 226 62 596.3 0 62 227 378 596.3 0 378 228 41 596.3 0 41 229 49 596.3 0 49 230 25 596.3 0 25 231 16 596.3 0 16 232 30 596.3 0 30 233 30 596.3 0 30 234 36 596.3 0 36 235 40 596.3 0 40 236 41 596.3 0 41 237 40 596.3 0 40 238 61 596.3 0 61 239 44 596.3 0 44 240 62 596.3 0 62 241 68 596.3 0 68 242 61 596.3 0 61 243 92 596.3 0 92 244 37 596.3 0 37 245 36 596.3 0 36 246 65 596.3 0 65 247 63 596.3 0 63 248 58 596.3 0 58 249 74 596.3 0 74 250 41 596.3 0 41 251 30 596.3 0 30 252 37 596.3 0 37 253 44 596.3 0 44 254 66 596.3 0 66 255 63 596.3 0 63 256 37 596.3 0 37 257 45 596.3 0 45 258 41 596.3 0 41 259 74 596.3 0 74 260 48 596.3 0 48 261 48 596.3 0 48 262 46 596.3 0 46 263 56 596.3 0 56 264 47 596.3 0 47 265 163 596.3 0 163 266 455 596.3 0 455 267 66 596.3 0 66 268 58 596.3 0 58 269 64 596.3 0 64 270 108 596.3 0 108 271 108 596.3 0 108 272 251 596.3 0 251 273 257 596.3 0 257 274 159 596.3 0 159 275 66 596.3 0 66 276 34 596.3 0 34 277 39 596.3 0 39 278 47 596.3 0 47 279 63 596.3 0 63 280 82 596.3 0 82 281 57 596.3 0 57 282 58 596.3 0 58 283 42 596.3 0 42 284 30 596.3 0 30 285 54 596.3 0 54 286 66 596.3 0 66 287 64 596.3 0 64 288 72 596.3 0 72 289 61 596.3 0 61 290 98 596.3 0 98 291 64 596.3 0 64 292 58 596.3 0 58 293 55 596.3 0 55 294 72 596.3 0 72 295 85 596.3 0 85 296 87 596.3 0 87 297 49 596.3 0 49 298 46 596.3 0 46 299 45 596.3 0 45 300 72 596.3 0 72 301 66 596.3 0 66 Finished in 9.09 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 201,636 Reads with adapters: 84,989 (42.1%) Reads that were too short: 47 (0.0%) Reads written (passing filters): 84,942 (42.1%) Total basepairs processed: 57,471,695 bp Total written (filtered): 23,526,365 bp (40.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 84989 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 22 0.0 3 0 0 22 16 412 0.0 3 0 394 16 2 17 84246 0.0 3 82439 1738 60 9 18 143 0.0 3 7 132 4 19 30 0.0 3 0 0 26 4 20 67 0.0 3 0 0 0 67 21 2 0.0 3 2 23 1 0.0 3 1 28 1 0.0 3 0 0 0 1 32 2 0.0 3 2 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 147 5 0.0 3 0 1 1 3 150 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 221 11 0.0 3 0 0 0 11 226 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 3 0.0 3 0 0 0 3 281 4 0.0 3 0 0 0 4 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.00 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 84,942 Reads with adapters: 201 (0.2%) Reads written (passing filters): 84,942 (100.0%) Total basepairs processed: 23,526,365 bp Total written (filtered): 23,525,710 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 201 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 28.4% C: 38.8% G: 21.9% T: 10.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 172 1327.2 0 172 4 14 331.8 0 14 5 13 83.0 0 13 6 1 20.7 0 1 12 1 0.0 1 0 1