This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.55 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 70,970 (2.9%) Reads that were too short: 6,054 (0.2%) Reads written (passing filters): 64,916 (2.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 14,955,455 bp (2.0%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 70970 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 246 596.3 0 246 7 9781 596.3 0 9781 8 286 596.3 0 286 9 10046 596.3 0 10046 10 24 596.3 0 24 11 136 596.3 0 136 12 41 596.3 0 41 13 33 596.3 0 33 14 25 596.3 0 25 15 35 596.3 0 35 16 115 596.3 0 115 17 162 596.3 0 162 18 130 596.3 0 130 19 25 596.3 0 25 20 31 596.3 0 31 21 33 596.3 0 33 22 59 596.3 0 59 23 30 596.3 0 30 24 31 596.3 0 31 25 26 596.3 0 26 26 19 596.3 0 19 27 32 596.3 0 32 28 35 596.3 0 35 29 30 596.3 0 30 30 23 596.3 0 23 31 36 596.3 0 36 32 48 596.3 0 48 33 53 596.3 0 53 34 30 596.3 0 30 35 30 596.3 0 30 36 27 596.3 0 27 37 33 596.3 0 33 38 36 596.3 0 36 39 44 596.3 0 44 40 31 596.3 0 31 41 34 596.3 0 34 42 47 596.3 0 47 43 49 596.3 0 49 44 47 596.3 0 47 45 32 596.3 0 32 46 336 596.3 0 336 47 284 596.3 0 284 48 870 596.3 0 870 49 38 596.3 0 38 50 35 596.3 0 35 51 29 596.3 0 29 52 40 596.3 0 40 53 24 596.3 0 24 54 33 596.3 0 33 55 60 596.3 0 60 56 34 596.3 0 34 57 36 596.3 0 36 58 31 596.3 0 31 59 48 596.3 0 48 60 38 596.3 0 38 61 45 596.3 0 45 62 47 596.3 0 47 63 51 596.3 0 51 64 37 596.3 0 37 65 41 596.3 0 41 66 35 596.3 0 35 67 41 596.3 0 41 68 48 596.3 0 48 69 57 596.3 0 57 70 60 596.3 0 60 71 48 596.3 0 48 72 44 596.3 0 44 73 45 596.3 0 45 74 62 596.3 0 62 75 45 596.3 0 45 76 56 596.3 0 56 77 66 596.3 0 66 78 73 596.3 0 73 79 183 596.3 0 183 80 494 596.3 0 494 81 694 596.3 0 694 82 151 596.3 0 151 83 200 596.3 0 200 84 224 596.3 0 224 85 110 596.3 0 110 86 151 596.3 0 151 87 141 596.3 0 141 88 121 596.3 0 121 89 156 596.3 0 156 90 162 596.3 0 162 91 175 596.3 0 175 92 294 596.3 0 294 93 418 596.3 0 418 94 6284 596.3 0 6284 95 5881 596.3 0 5881 96 11654 596.3 0 11654 97 1649 596.3 0 1649 98 2675 596.3 0 2675 99 38 596.3 0 38 100 26 596.3 0 26 101 13 596.3 0 13 102 29 596.3 0 29 103 25 596.3 0 25 104 18 596.3 0 18 105 35 596.3 0 35 106 37 596.3 0 37 107 37 596.3 0 37 108 28 596.3 0 28 109 33 596.3 0 33 110 82 596.3 0 82 111 77 596.3 0 77 112 182 596.3 0 182 113 25 596.3 0 25 114 28 596.3 0 28 115 24 596.3 0 24 116 35 596.3 0 35 117 51 596.3 0 51 118 99 596.3 0 99 119 77 596.3 0 77 120 53 596.3 0 53 121 47 596.3 0 47 122 37 596.3 0 37 123 55 596.3 0 55 124 124 596.3 0 124 125 403 596.3 0 403 126 415 596.3 0 415 127 38 596.3 0 38 128 40 596.3 0 40 129 46 596.3 0 46 130 100 596.3 0 100 131 183 596.3 0 183 132 229 596.3 0 229 133 188 596.3 0 188 134 39 596.3 0 39 135 55 596.3 0 55 136 47 596.3 0 47 137 89 596.3 0 89 138 174 596.3 0 174 139 260 596.3 0 260 140 119 596.3 0 119 141 93 596.3 0 93 142 83 596.3 0 83 143 132 596.3 0 132 144 85 596.3 0 85 145 93 596.3 0 93 146 173 596.3 0 173 147 135 596.3 0 135 148 71 596.3 0 71 149 52 596.3 0 52 150 42 596.3 0 42 151 36 596.3 0 36 152 38 596.3 0 38 153 34 596.3 0 34 154 37 596.3 0 37 155 39 596.3 0 39 156 45 596.3 0 45 157 70 596.3 0 70 158 467 596.3 0 467 159 292 596.3 0 292 160 490 596.3 0 490 161 50 596.3 0 50 162 43 596.3 0 43 163 35 596.3 0 35 164 31 596.3 0 31 165 44 596.3 0 44 166 37 596.3 0 37 167 26 596.3 0 26 168 38 596.3 0 38 169 25 596.3 0 25 170 25 596.3 0 25 171 25 596.3 0 25 172 31 596.3 0 31 173 31 596.3 0 31 174 28 596.3 0 28 175 28 596.3 0 28 176 37 596.3 0 37 177 33 596.3 0 33 178 35 596.3 0 35 179 140 596.3 0 140 180 29 596.3 0 29 181 69 596.3 0 69 182 41 596.3 0 41 183 55 596.3 0 55 184 49 596.3 0 49 185 189 596.3 0 189 186 69 596.3 0 69 187 71 596.3 0 71 188 70 596.3 0 70 189 35 596.3 0 35 190 41 596.3 0 41 191 42 596.3 0 42 192 78 596.3 0 78 193 93 596.3 0 93 194 86 596.3 0 86 195 43 596.3 0 43 196 36 596.3 0 36 197 35 596.3 0 35 198 25 596.3 0 25 199 27 596.3 0 27 200 40 596.3 0 40 201 39 596.3 0 39 202 40 596.3 0 40 203 46 596.3 0 46 204 46 596.3 0 46 205 216 596.3 0 216 206 152 596.3 0 152 207 429 596.3 0 429 208 45 596.3 0 45 209 42 596.3 0 42 210 31 596.3 0 31 211 46 596.3 0 46 212 47 596.3 0 47 213 28 596.3 0 28 214 22 596.3 0 22 215 29 596.3 0 29 216 28 596.3 0 28 217 20 596.3 0 20 218 25 596.3 0 25 219 36 596.3 0 36 220 21 596.3 0 21 221 39 596.3 0 39 222 27 596.3 0 27 223 33 596.3 0 33 224 28 596.3 0 28 225 22 596.3 0 22 226 27 596.3 0 27 227 45 596.3 0 45 228 64 596.3 0 64 229 40 596.3 0 40 230 24 596.3 0 24 231 31 596.3 0 31 232 38 596.3 0 38 233 49 596.3 0 49 234 33 596.3 0 33 235 28 596.3 0 28 236 33 596.3 0 33 237 36 596.3 0 36 238 38 596.3 0 38 239 40 596.3 0 40 240 37 596.3 0 37 241 52 596.3 0 52 242 34 596.3 0 34 243 34 596.3 0 34 244 28 596.3 0 28 245 30 596.3 0 30 246 31 596.3 0 31 247 39 596.3 0 39 248 30 596.3 0 30 249 38 596.3 0 38 250 34 596.3 0 34 251 44 596.3 0 44 252 24 596.3 0 24 253 63 596.3 0 63 254 77 596.3 0 77 255 80 596.3 0 80 256 111 596.3 0 111 257 54 596.3 0 54 258 41 596.3 0 41 259 58 596.3 0 58 260 40 596.3 0 40 261 67 596.3 0 67 262 80 596.3 0 80 263 87 596.3 0 87 264 50 596.3 0 50 265 44 596.3 0 44 266 36 596.3 0 36 267 57 596.3 0 57 268 41 596.3 0 41 269 45 596.3 0 45 270 150 596.3 0 150 271 136 596.3 0 136 272 107 596.3 0 107 273 85 596.3 0 85 274 66 596.3 0 66 275 49 596.3 0 49 276 68 596.3 0 68 277 61 596.3 0 61 278 37 596.3 0 37 279 51 596.3 0 51 280 41 596.3 0 41 281 104 596.3 0 104 282 81 596.3 0 81 283 114 596.3 0 114 284 69 596.3 0 69 285 41 596.3 0 41 286 71 596.3 0 71 287 87 596.3 0 87 288 118 596.3 0 118 289 74 596.3 0 74 290 67 596.3 0 67 291 61 596.3 0 61 292 64 596.3 0 64 293 48 596.3 0 48 294 70 596.3 0 70 295 87 596.3 0 87 296 94 596.3 0 94 297 57 596.3 0 57 298 73 596.3 0 73 299 118 596.3 0 118 300 145 596.3 0 145 301 50 596.3 0 50 Finished in 3.12 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 64,916 Reads with adapters: 9,937 (15.3%) Reads that were too short: 189 (0.3%) Reads written (passing filters): 9,748 (15.0%) Total basepairs processed: 14,955,455 bp Total written (filtered): 2,668,810 bp (17.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 9937 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 3 0.0 3 0 0 3 19 135 0.0 3 0 119 9 5 2 20 9539 0.0 4 8971 469 75 17 7 21 19 0.0 4 2 14 2 1 22 15 0.0 4 7 3 1 3 1 23 11 0.0 4 0 0 0 8 3 24 1 0.0 4 1 25 1 0.0 4 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 42 1 0.0 4 1 48 2 0.0 4 0 0 0 0 2 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 115 3 0.0 4 0 0 0 0 3 116 1 0.0 4 0 0 0 0 1 117 8 0.0 4 0 0 0 0 8 120 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 141 126 0.0 4 0 0 0 8 118 142 26 0.0 4 0 0 0 2 24 143 6 0.0 4 0 0 0 0 6 144 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 190 2 0.0 4 0 0 0 0 2 191 1 0.0 4 0 0 0 1 205 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 9,748 Reads with adapters: 308 (3.2%) Reads written (passing filters): 9,748 (100.0%) Total basepairs processed: 2,668,810 bp Total written (filtered): 2,664,615 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 308 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.4% C: 18.5% G: 67.9% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 42 152.3 0 42 4 6 38.1 0 6 9 4 0.0 0 0 4 10 6 0.0 1 0 6 11 119 0.0 1 3 116 12 112 0.0 1 1 111 13 10 0.0 1 0 10 14 2 0.0 1 0 2 152 6 0.0 1 5 1 226 1 0.0 1 1