This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATGGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.43 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 67,904 (2.8%) Reads that were too short: 3,582 (0.1%) Reads written (passing filters): 64,322 (2.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 15,024,771 bp (2.0%) === Adapter 1 === Sequence: ATGGAT; Type: regular 5'; Length: 6; Trimmed: 67904 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 160 596.3 0 160 7 12126 596.3 0 12126 8 173 596.3 0 173 9 9192 596.3 0 9192 10 19 596.3 0 19 11 120 596.3 0 120 12 28 596.3 0 28 13 35 596.3 0 35 14 36 596.3 0 36 15 27 596.3 0 27 16 102 596.3 0 102 17 124 596.3 0 124 18 87 596.3 0 87 19 29 596.3 0 29 20 25 596.3 0 25 21 32 596.3 0 32 22 24 596.3 0 24 23 26 596.3 0 26 24 31 596.3 0 31 25 23 596.3 0 23 26 31 596.3 0 31 27 23 596.3 0 23 28 27 596.3 0 27 29 21 596.3 0 21 30 17 596.3 0 17 31 26 596.3 0 26 32 22 596.3 0 22 33 28 596.3 0 28 34 29 596.3 0 29 35 23 596.3 0 23 36 33 596.3 0 33 37 34 596.3 0 34 38 29 596.3 0 29 39 30 596.3 0 30 40 20 596.3 0 20 41 32 596.3 0 32 42 43 596.3 0 43 43 34 596.3 0 34 44 41 596.3 0 41 45 41 596.3 0 41 46 274 596.3 0 274 47 236 596.3 0 236 48 796 596.3 0 796 49 46 596.3 0 46 50 38 596.3 0 38 51 32 596.3 0 32 52 41 596.3 0 41 53 23 596.3 0 23 54 24 596.3 0 24 55 40 596.3 0 40 56 22 596.3 0 22 57 22 596.3 0 22 58 36 596.3 0 36 59 43 596.3 0 43 60 31 596.3 0 31 61 30 596.3 0 30 62 33 596.3 0 33 63 46 596.3 0 46 64 27 596.3 0 27 65 25 596.3 0 25 66 39 596.3 0 39 67 30 596.3 0 30 68 23 596.3 0 23 69 52 596.3 0 52 70 34 596.3 0 34 71 47 596.3 0 47 72 29 596.3 0 29 73 32 596.3 0 32 74 37 596.3 0 37 75 35 596.3 0 35 76 39 596.3 0 39 77 40 596.3 0 40 78 65 596.3 0 65 79 145 596.3 0 145 80 351 596.3 0 351 81 548 596.3 0 548 82 133 596.3 0 133 83 190 596.3 0 190 84 188 596.3 0 188 85 86 596.3 0 86 86 53 596.3 0 53 87 102 596.3 0 102 88 88 596.3 0 88 89 139 596.3 0 139 90 131 596.3 0 131 91 166 596.3 0 166 92 238 596.3 0 238 93 283 596.3 0 283 94 5935 596.3 0 5935 95 5985 596.3 0 5985 96 12598 596.3 0 12598 97 2037 596.3 0 2037 98 3400 596.3 0 3400 99 41 596.3 0 41 100 23 596.3 0 23 101 29 596.3 0 29 102 24 596.3 0 24 103 27 596.3 0 27 104 21 596.3 0 21 105 28 596.3 0 28 106 29 596.3 0 29 107 45 596.3 0 45 108 30 596.3 0 30 109 38 596.3 0 38 110 87 596.3 0 87 111 84 596.3 0 84 112 176 596.3 0 176 113 25 596.3 0 25 114 28 596.3 0 28 115 26 596.3 0 26 116 34 596.3 0 34 117 24 596.3 0 24 118 73 596.3 0 73 119 44 596.3 0 44 120 55 596.3 0 55 121 23 596.3 0 23 122 29 596.3 0 29 123 45 596.3 0 45 124 99 596.3 0 99 125 277 596.3 0 277 126 258 596.3 0 258 127 22 596.3 0 22 128 34 596.3 0 34 129 36 596.3 0 36 130 55 596.3 0 55 131 106 596.3 0 106 132 130 596.3 0 130 133 96 596.3 0 96 134 28 596.3 0 28 135 45 596.3 0 45 136 36 596.3 0 36 137 97 596.3 0 97 138 158 596.3 0 158 139 274 596.3 0 274 140 91 596.3 0 91 141 89 596.3 0 89 142 80 596.3 0 80 143 123 596.3 0 123 144 85 596.3 0 85 145 50 596.3 0 50 146 110 596.3 0 110 147 115 596.3 0 115 148 52 596.3 0 52 149 33 596.3 0 33 150 16 596.3 0 16 151 28 596.3 0 28 152 12 596.3 0 12 153 19 596.3 0 19 154 14 596.3 0 14 155 22 596.3 0 22 156 36 596.3 0 36 157 51 596.3 0 51 158 400 596.3 0 400 159 249 596.3 0 249 160 486 596.3 0 486 161 30 596.3 0 30 162 15 596.3 0 15 163 8 596.3 0 8 164 9 596.3 0 9 165 21 596.3 0 21 166 18 596.3 0 18 167 21 596.3 0 21 168 17 596.3 0 17 169 11 596.3 0 11 170 8 596.3 0 8 171 22 596.3 0 22 172 17 596.3 0 17 173 25 596.3 0 25 174 17 596.3 0 17 175 19 596.3 0 19 176 18 596.3 0 18 177 20 596.3 0 20 178 20 596.3 0 20 179 103 596.3 0 103 180 11 596.3 0 11 181 26 596.3 0 26 182 15 596.3 0 15 183 33 596.3 0 33 184 29 596.3 0 29 185 226 596.3 0 226 186 34 596.3 0 34 187 36 596.3 0 36 188 29 596.3 0 29 189 20 596.3 0 20 190 14 596.3 0 14 191 13 596.3 0 13 192 38 596.3 0 38 193 39 596.3 0 39 194 48 596.3 0 48 195 16 596.3 0 16 196 25 596.3 0 25 197 17 596.3 0 17 198 18 596.3 0 18 199 14 596.3 0 14 200 20 596.3 0 20 201 16 596.3 0 16 202 16 596.3 0 16 203 14 596.3 0 14 204 32 596.3 0 32 205 182 596.3 0 182 206 134 596.3 0 134 207 469 596.3 0 469 208 15 596.3 0 15 209 15 596.3 0 15 210 17 596.3 0 17 211 16 596.3 0 16 212 15 596.3 0 15 213 6 596.3 0 6 214 9 596.3 0 9 215 12 596.3 0 12 216 13 596.3 0 13 217 19 596.3 0 19 218 14 596.3 0 14 219 20 596.3 0 20 220 15 596.3 0 15 221 12 596.3 0 12 222 19 596.3 0 19 223 20 596.3 0 20 224 15 596.3 0 15 225 13 596.3 0 13 226 17 596.3 0 17 227 20 596.3 0 20 228 30 596.3 0 30 229 22 596.3 0 22 230 10 596.3 0 10 231 15 596.3 0 15 232 35 596.3 0 35 233 23 596.3 0 23 234 20 596.3 0 20 235 5 596.3 0 5 236 11 596.3 0 11 237 23 596.3 0 23 238 28 596.3 0 28 239 16 596.3 0 16 240 32 596.3 0 32 241 22 596.3 0 22 242 21 596.3 0 21 243 17 596.3 0 17 244 12 596.3 0 12 245 27 596.3 0 27 246 10 596.3 0 10 247 12 596.3 0 12 248 10 596.3 0 10 249 21 596.3 0 21 250 20 596.3 0 20 251 27 596.3 0 27 252 24 596.3 0 24 253 60 596.3 0 60 254 55 596.3 0 55 255 40 596.3 0 40 256 52 596.3 0 52 257 37 596.3 0 37 258 26 596.3 0 26 259 30 596.3 0 30 260 15 596.3 0 15 261 30 596.3 0 30 262 45 596.3 0 45 263 50 596.3 0 50 264 24 596.3 0 24 265 18 596.3 0 18 266 20 596.3 0 20 267 26 596.3 0 26 268 20 596.3 0 20 269 26 596.3 0 26 270 96 596.3 0 96 271 58 596.3 0 58 272 67 596.3 0 67 273 31 596.3 0 31 274 23 596.3 0 23 275 17 596.3 0 17 276 18 596.3 0 18 277 36 596.3 0 36 278 23 596.3 0 23 279 30 596.3 0 30 280 28 596.3 0 28 281 85 596.3 0 85 282 50 596.3 0 50 283 74 596.3 0 74 284 23 596.3 0 23 285 34 596.3 0 34 286 37 596.3 0 37 287 57 596.3 0 57 288 71 596.3 0 71 289 50 596.3 0 50 290 32 596.3 0 32 291 32 596.3 0 32 292 42 596.3 0 42 293 37 596.3 0 37 294 22 596.3 0 22 295 39 596.3 0 39 296 43 596.3 0 43 297 21 596.3 0 21 298 38 596.3 0 38 299 48 596.3 0 48 300 70 596.3 0 70 301 34 596.3 0 34 Finished in 2.75 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 64,322 Reads with adapters: 9,446 (14.7%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 9,431 (14.7%) Total basepairs processed: 15,024,771 bp Total written (filtered): 2,588,644 bp (17.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9446 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 60 0.0 3 0 59 1 17 9287 0.0 3 9076 204 6 1 18 14 0.0 3 0 13 1 19 2 0.0 3 1 0 0 1 20 29 0.0 3 0 0 0 29 22 1 0.0 3 0 0 0 1 26 1 0.0 3 1 30 7 0.0 3 0 0 0 7 33 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 6 0.0 3 0 0 0 6 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.33 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 9,431 Reads with adapters: 111 (1.2%) Reads written (passing filters): 9,431 (100.0%) Total basepairs processed: 2,588,644 bp Total written (filtered): 2,588,308 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 111 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 38.7% C: 6.3% G: 54.1% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 108 147.4 0 108 4 3 36.8 0 3