This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.02 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 202,829 (8.3%) Reads that were too short: 11,000 (0.5%) Reads written (passing filters): 191,829 (7.9%) Total basepairs processed: 735,190,694 bp Total written (filtered): 36,337,185 bp (4.9%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 202829 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 279 596.3 0 279 7 10033 596.3 0 10033 8 318 596.3 0 318 9 11485 596.3 0 11485 10 25 596.3 0 25 11 19 596.3 0 19 12 12 596.3 0 12 13 16 596.3 0 16 14 10 596.3 0 10 15 35 596.3 0 35 16 18 596.3 0 18 17 20 596.3 0 20 18 9 596.3 0 9 19 14 596.3 0 14 20 11 596.3 0 11 21 25 596.3 0 25 22 21 596.3 0 21 23 18 596.3 0 18 24 23 596.3 0 23 25 35 596.3 0 35 26 26 596.3 0 26 27 23 596.3 0 23 28 18 596.3 0 18 29 15 596.3 0 15 30 23 596.3 0 23 31 31 596.3 0 31 32 29 596.3 0 29 33 32 596.3 0 32 34 21 596.3 0 21 35 25 596.3 0 25 36 40 596.3 0 40 37 21 596.3 0 21 38 23 596.3 0 23 39 78 596.3 0 78 40 47 596.3 0 47 41 22 596.3 0 22 42 25 596.3 0 25 43 22 596.3 0 22 44 20 596.3 0 20 45 33 596.3 0 33 46 14 596.3 0 14 47 22 596.3 0 22 48 28 596.3 0 28 49 25 596.3 0 25 50 17 596.3 0 17 51 54 596.3 0 54 52 33 596.3 0 33 53 34 596.3 0 34 54 34 596.3 0 34 55 42 596.3 0 42 56 77 596.3 0 77 57 96 596.3 0 96 58 85 596.3 0 85 59 146 596.3 0 146 60 71 596.3 0 71 61 812 596.3 0 812 62 48 596.3 0 48 63 36 596.3 0 36 64 33 596.3 0 33 65 23 596.3 0 23 66 32 596.3 0 32 67 24 596.3 0 24 68 34 596.3 0 34 69 34 596.3 0 34 70 51 596.3 0 51 71 53 596.3 0 53 72 84 596.3 0 84 73 35 596.3 0 35 74 36 596.3 0 36 75 25 596.3 0 25 76 50 596.3 0 50 77 216 596.3 0 216 78 37 596.3 0 37 79 28 596.3 0 28 80 42 596.3 0 42 81 28 596.3 0 28 82 33 596.3 0 33 83 30 596.3 0 30 84 72 596.3 0 72 85 50 596.3 0 50 86 66 596.3 0 66 87 195 596.3 0 195 88 35 596.3 0 35 89 34 596.3 0 34 90 46 596.3 0 46 91 43 596.3 0 43 92 37 596.3 0 37 93 48 596.3 0 48 94 325 596.3 0 325 95 55 596.3 0 55 96 147 596.3 0 147 97 349 596.3 0 349 98 75 596.3 0 75 99 153 596.3 0 153 100 73 596.3 0 73 101 79 596.3 0 79 102 240 596.3 0 240 103 102 596.3 0 102 104 112 596.3 0 112 105 83 596.3 0 83 106 203 596.3 0 203 107 269 596.3 0 269 108 333 596.3 0 333 109 405 596.3 0 405 110 585 596.3 0 585 111 603 596.3 0 603 112 1264 596.3 0 1264 113 2463 596.3 0 2463 114 36153 596.3 0 36153 115 19193 596.3 0 19193 116 50060 596.3 0 50060 117 236 596.3 0 236 118 118 596.3 0 118 119 44 596.3 0 44 120 53 596.3 0 53 121 22 596.3 0 22 122 21 596.3 0 21 123 39 596.3 0 39 124 28 596.3 0 28 125 33 596.3 0 33 126 91 596.3 0 91 127 203 596.3 0 203 128 144 596.3 0 144 129 136 596.3 0 136 130 95 596.3 0 95 131 82 596.3 0 82 132 35 596.3 0 35 133 32 596.3 0 32 134 54 596.3 0 54 135 127 596.3 0 127 136 183 596.3 0 183 137 61 596.3 0 61 138 73 596.3 0 73 139 52 596.3 0 52 140 155 596.3 0 155 141 414 596.3 0 414 142 251 596.3 0 251 143 396 596.3 0 396 144 162 596.3 0 162 145 208 596.3 0 208 146 251 596.3 0 251 147 2928 596.3 0 2928 148 5610 596.3 0 5610 149 4302 596.3 0 4302 150 212 596.3 0 212 151 370 596.3 0 370 152 1150 596.3 0 1150 153 12734 596.3 0 12734 154 4127 596.3 0 4127 155 13354 596.3 0 13354 156 285 596.3 0 285 157 161 596.3 0 161 158 87 596.3 0 87 159 36 596.3 0 36 160 70 596.3 0 70 161 39 596.3 0 39 162 60 596.3 0 60 163 51 596.3 0 51 164 31 596.3 0 31 165 46 596.3 0 46 166 26 596.3 0 26 167 35 596.3 0 35 168 94 596.3 0 94 169 186 596.3 0 186 170 133 596.3 0 133 171 93 596.3 0 93 172 28 596.3 0 28 173 23 596.3 0 23 174 17 596.3 0 17 175 26 596.3 0 26 176 30 596.3 0 30 177 38 596.3 0 38 178 24 596.3 0 24 179 17 596.3 0 17 180 92 596.3 0 92 181 101 596.3 0 101 182 63 596.3 0 63 183 509 596.3 0 509 184 227 596.3 0 227 185 118 596.3 0 118 186 205 596.3 0 205 187 64 596.3 0 64 188 39 596.3 0 39 189 118 596.3 0 118 190 163 596.3 0 163 191 65 596.3 0 65 192 55 596.3 0 55 193 25 596.3 0 25 194 18 596.3 0 18 195 16 596.3 0 16 196 17 596.3 0 17 197 27 596.3 0 27 198 25 596.3 0 25 199 46 596.3 0 46 200 28 596.3 0 28 201 34 596.3 0 34 202 98 596.3 0 98 203 54 596.3 0 54 204 139 596.3 0 139 205 25 596.3 0 25 206 18 596.3 0 18 207 29 596.3 0 29 208 26 596.3 0 26 209 19 596.3 0 19 210 21 596.3 0 21 211 14 596.3 0 14 212 24 596.3 0 24 213 35 596.3 0 35 214 20 596.3 0 20 215 36 596.3 0 36 216 298 596.3 0 298 217 145 596.3 0 145 218 289 596.3 0 289 219 23 596.3 0 23 220 20 596.3 0 20 221 28 596.3 0 28 222 24 596.3 0 24 223 18 596.3 0 18 224 25 596.3 0 25 225 17 596.3 0 17 226 78 596.3 0 78 227 42 596.3 0 42 228 76 596.3 0 76 229 32 596.3 0 32 230 28 596.3 0 28 231 39 596.3 0 39 232 36 596.3 0 36 233 37 596.3 0 37 234 49 596.3 0 49 235 100 596.3 0 100 236 52 596.3 0 52 237 35 596.3 0 35 238 35 596.3 0 35 239 28 596.3 0 28 240 21 596.3 0 21 241 23 596.3 0 23 242 26 596.3 0 26 243 22 596.3 0 22 244 43 596.3 0 43 245 28 596.3 0 28 246 43 596.3 0 43 247 27 596.3 0 27 248 30 596.3 0 30 249 51 596.3 0 51 250 67 596.3 0 67 251 68 596.3 0 68 252 20 596.3 0 20 253 36 596.3 0 36 254 39 596.3 0 39 255 47 596.3 0 47 256 36 596.3 0 36 257 36 596.3 0 36 258 26 596.3 0 26 259 45 596.3 0 45 260 33 596.3 0 33 261 39 596.3 0 39 262 46 596.3 0 46 263 33 596.3 0 33 264 40 596.3 0 40 265 22 596.3 0 22 266 50 596.3 0 50 267 61 596.3 0 61 268 56 596.3 0 56 269 51 596.3 0 51 270 88 596.3 0 88 271 106 596.3 0 106 272 84 596.3 0 84 273 63 596.3 0 63 274 136 596.3 0 136 275 97 596.3 0 97 276 84 596.3 0 84 277 104 596.3 0 104 278 122 596.3 0 122 279 495 596.3 0 495 280 532 596.3 0 532 281 1142 596.3 0 1142 282 1471 596.3 0 1471 283 1151 596.3 0 1151 284 83 596.3 0 83 285 90 596.3 0 90 286 70 596.3 0 70 287 48 596.3 0 48 288 53 596.3 0 53 289 63 596.3 0 63 290 311 596.3 0 311 291 233 596.3 0 233 292 364 596.3 0 364 293 153 596.3 0 153 294 105 596.3 0 105 295 115 596.3 0 115 296 96 596.3 0 96 297 106 596.3 0 106 298 79 596.3 0 79 299 36 596.3 0 36 300 22 596.3 0 22 301 21 596.3 0 21 Finished in 6.79 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 191,829 Reads with adapters: 10,054 (5.2%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 10,005 (5.2%) Total basepairs processed: 36,337,185 bp Total written (filtered): 2,763,535 bp (7.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10054 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 80 0.0 3 0 77 3 17 9829 0.0 3 9583 243 3 18 29 0.0 3 2 27 19 5 0.0 3 0 0 4 1 20 10 0.0 3 1 0 0 9 32 1 0.0 3 0 0 0 1 36 1 0.0 3 1 38 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 50 8 0.0 3 0 0 0 8 51 6 0.0 3 0 0 0 6 53 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 1 1 63 1 0.0 3 0 0 0 1 68 2 0.0 3 0 0 0 2 71 1 0.0 3 1 79 1 0.0 3 0 0 0 1 85 14 0.0 3 0 0 0 14 86 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 100 1 0.0 3 1 109 1 0.0 3 0 0 0 1 110 8 0.0 3 0 0 1 7 111 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 136 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 172 5 0.0 3 0 0 0 5 173 1 0.0 3 0 0 0 1 174 5 0.0 3 0 0 0 5 175 3 0.0 3 0 0 0 3 176 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 249 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.34 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 10,005 Reads with adapters: 17 (0.2%) Reads written (passing filters): 10,005 (100.0%) Total basepairs processed: 2,763,535 bp Total written (filtered): 2,763,484 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 17 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.6% C: 58.8% G: 11.8% T: 11.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17 156.3 0 17