This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.48 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 82,950 (3.4%) Reads that were too short: 60,296 (2.5%) Reads written (passing filters): 22,654 (0.9%) Total basepairs processed: 735,190,694 bp Total written (filtered): 5,910,533 bp (0.8%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 82950 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 283 596.3 0 283 7 8584 596.3 0 8584 8 153 596.3 0 153 9 5992 596.3 0 5992 10 42 596.3 0 42 11 45 596.3 0 45 12 247 596.3 0 247 13 32 596.3 0 32 14 29 596.3 0 29 15 50 596.3 0 50 16 19 596.3 0 19 17 26 596.3 0 26 18 38 596.3 0 38 19 26 596.3 0 26 20 28 596.3 0 28 21 30 596.3 0 30 22 23 596.3 0 23 23 21 596.3 0 21 24 26 596.3 0 26 25 26 596.3 0 26 26 33 596.3 0 33 27 32 596.3 0 32 28 28 596.3 0 28 29 42 596.3 0 42 30 35 596.3 0 35 31 24 596.3 0 24 32 23 596.3 0 23 33 53 596.3 0 53 34 30 596.3 0 30 35 27 596.3 0 27 36 44 596.3 0 44 37 27 596.3 0 27 38 41 596.3 0 41 39 42 596.3 0 42 40 40 596.3 0 40 41 31 596.3 0 31 42 41 596.3 0 41 43 44 596.3 0 44 44 22 596.3 0 22 45 17 596.3 0 17 46 27 596.3 0 27 47 31 596.3 0 31 48 36 596.3 0 36 49 35 596.3 0 35 50 19 596.3 0 19 51 33 596.3 0 33 52 49 596.3 0 49 53 128 596.3 0 128 54 50 596.3 0 50 55 19 596.3 0 19 56 31 596.3 0 31 57 57 596.3 0 57 58 27 596.3 0 27 59 34 596.3 0 34 60 39 596.3 0 39 61 30 596.3 0 30 62 44 596.3 0 44 63 28 596.3 0 28 64 43 596.3 0 43 65 30 596.3 0 30 66 27 596.3 0 27 67 30 596.3 0 30 68 21 596.3 0 21 69 46 596.3 0 46 70 38 596.3 0 38 71 35 596.3 0 35 72 65 596.3 0 65 73 113 596.3 0 113 74 26 596.3 0 26 75 46 596.3 0 46 76 20 596.3 0 20 77 26 596.3 0 26 78 25 596.3 0 25 79 23 596.3 0 23 80 21 596.3 0 21 81 27 596.3 0 27 82 21 596.3 0 21 83 24 596.3 0 24 84 29 596.3 0 29 85 22 596.3 0 22 86 22 596.3 0 22 87 24 596.3 0 24 88 20 596.3 0 20 89 32 596.3 0 32 90 21 596.3 0 21 91 44 596.3 0 44 92 38 596.3 0 38 93 25 596.3 0 25 94 40 596.3 0 40 95 30 596.3 0 30 96 29 596.3 0 29 97 20 596.3 0 20 98 23 596.3 0 23 99 25 596.3 0 25 100 27 596.3 0 27 101 22 596.3 0 22 102 20 596.3 0 20 103 39 596.3 0 39 104 23 596.3 0 23 105 19 596.3 0 19 106 25 596.3 0 25 107 15 596.3 0 15 108 39 596.3 0 39 109 27 596.3 0 27 110 23 596.3 0 23 111 23 596.3 0 23 112 15 596.3 0 15 113 24 596.3 0 24 114 23 596.3 0 23 115 17 596.3 0 17 116 23 596.3 0 23 117 39 596.3 0 39 118 30 596.3 0 30 119 29 596.3 0 29 120 38 596.3 0 38 121 29 596.3 0 29 122 15 596.3 0 15 123 192 596.3 0 192 124 33 596.3 0 33 125 35 596.3 0 35 126 28 596.3 0 28 127 18 596.3 0 18 128 68 596.3 0 68 129 33 596.3 0 33 130 324 596.3 0 324 131 268 596.3 0 268 132 490 596.3 0 490 133 163 596.3 0 163 134 57 596.3 0 57 135 34 596.3 0 34 136 23 596.3 0 23 137 184 596.3 0 184 138 251 596.3 0 251 139 115 596.3 0 115 140 18 596.3 0 18 141 21 596.3 0 21 142 28 596.3 0 28 143 18 596.3 0 18 144 15 596.3 0 15 145 13 596.3 0 13 146 21 596.3 0 21 147 26 596.3 0 26 148 15 596.3 0 15 149 18 596.3 0 18 150 29 596.3 0 29 151 75 596.3 0 75 152 48 596.3 0 48 153 30 596.3 0 30 154 20 596.3 0 20 155 20 596.3 0 20 156 24 596.3 0 24 157 23 596.3 0 23 158 15 596.3 0 15 159 22 596.3 0 22 160 26 596.3 0 26 161 18 596.3 0 18 162 21 596.3 0 21 163 13 596.3 0 13 164 19 596.3 0 19 165 12 596.3 0 12 166 26 596.3 0 26 167 17 596.3 0 17 168 22 596.3 0 22 169 20 596.3 0 20 170 16 596.3 0 16 171 15 596.3 0 15 172 12 596.3 0 12 173 15 596.3 0 15 174 15 596.3 0 15 175 11 596.3 0 11 176 16 596.3 0 16 177 54 596.3 0 54 178 38 596.3 0 38 179 51 596.3 0 51 180 20 596.3 0 20 181 22 596.3 0 22 182 15 596.3 0 15 183 26 596.3 0 26 184 29 596.3 0 29 185 23 596.3 0 23 186 34 596.3 0 34 187 42 596.3 0 42 188 40 596.3 0 40 189 41 596.3 0 41 190 19 596.3 0 19 191 16 596.3 0 16 192 19 596.3 0 19 193 34 596.3 0 34 194 16 596.3 0 16 195 21 596.3 0 21 196 24 596.3 0 24 197 22 596.3 0 22 198 24 596.3 0 24 199 21 596.3 0 21 200 28 596.3 0 28 201 18 596.3 0 18 202 24 596.3 0 24 203 22 596.3 0 22 204 25 596.3 0 25 205 24 596.3 0 24 206 29 596.3 0 29 207 29 596.3 0 29 208 18 596.3 0 18 209 17 596.3 0 17 210 26 596.3 0 26 211 28 596.3 0 28 212 206 596.3 0 206 213 137 596.3 0 137 214 119 596.3 0 119 215 19 596.3 0 19 216 31 596.3 0 31 217 46 596.3 0 46 218 81 596.3 0 81 219 45 596.3 0 45 220 70 596.3 0 70 221 36 596.3 0 36 222 17 596.3 0 17 223 18 596.3 0 18 224 20 596.3 0 20 225 23 596.3 0 23 226 24 596.3 0 24 227 15 596.3 0 15 228 25 596.3 0 25 229 28 596.3 0 28 230 23 596.3 0 23 231 22 596.3 0 22 232 37 596.3 0 37 233 38 596.3 0 38 234 35 596.3 0 35 235 137 596.3 0 137 236 82 596.3 0 82 237 114 596.3 0 114 238 239 596.3 0 239 239 59 596.3 0 59 240 23 596.3 0 23 241 52 596.3 0 52 242 52 596.3 0 52 243 186 596.3 0 186 244 502 596.3 0 502 245 400 596.3 0 400 246 226 596.3 0 226 247 105 596.3 0 105 248 66 596.3 0 66 249 44 596.3 0 44 250 63 596.3 0 63 251 66 596.3 0 66 252 50 596.3 0 50 253 48 596.3 0 48 254 75 596.3 0 75 255 80 596.3 0 80 256 201 596.3 0 201 257 329 596.3 0 329 258 111 596.3 0 111 259 143 596.3 0 143 260 717 596.3 0 717 261 1976 596.3 0 1976 262 1119 596.3 0 1119 263 328 596.3 0 328 264 43 596.3 0 43 265 74 596.3 0 74 266 102 596.3 0 102 267 64 596.3 0 64 268 89 596.3 0 89 269 59 596.3 0 59 270 80 596.3 0 80 271 114 596.3 0 114 272 217 596.3 0 217 273 381 596.3 0 381 274 108 596.3 0 108 275 160 596.3 0 160 276 286 596.3 0 286 277 124 596.3 0 124 278 258 596.3 0 258 279 236 596.3 0 236 280 342 596.3 0 342 281 326 596.3 0 326 282 331 596.3 0 331 283 315 596.3 0 315 284 433 596.3 0 433 285 790 596.3 0 790 286 18045 596.3 0 18045 287 14332 596.3 0 14332 288 11910 596.3 0 11910 289 64 596.3 0 64 290 33 596.3 0 33 291 65 596.3 0 65 292 64 596.3 0 64 293 67 596.3 0 67 294 210 596.3 0 210 295 210 596.3 0 210 296 219 596.3 0 219 297 329 596.3 0 329 298 316 596.3 0 316 299 265 596.3 0 265 300 265 596.3 0 265 301 20 596.3 0 20 Finished in 1.09 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 22,654 Reads with adapters: 7,741 (34.2%) Reads that were too short: 23 (0.1%) Reads written (passing filters): 7,718 (34.1%) Total basepairs processed: 5,910,533 bp Total written (filtered): 2,120,832 bp (35.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 7741 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 45 0.0 3 0 45 17 6074 0.0 3 5907 162 3 2 18 43 0.0 3 1 39 3 19 1533 0.0 3 1496 33 4 20 4 0.0 3 0 3 0 1 21 2 0.0 3 0 0 2 22 3 0.0 3 0 0 0 3 28 1 0.0 3 0 0 0 1 41 6 0.0 3 0 0 0 6 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 145 1 0.0 3 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 1 164 2 0.0 3 0 0 0 2 170 2 0.0 3 0 0 0 2 171 7 0.0 3 0 0 0 7 173 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.28 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 7,718 Reads with adapters: 62 (0.8%) Reads written (passing filters): 7,718 (100.0%) Total basepairs processed: 2,120,832 bp Total written (filtered): 2,120,642 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 62 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 25.8% G: 19.4% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 58 120.6 0 58 4 4 30.1 0 4