This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.94 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 109,394 (4.5%) Reads that were too short: 19,785 (0.8%) Reads written (passing filters): 89,609 (3.7%) Total basepairs processed: 735,190,694 bp Total written (filtered): 24,074,678 bp (3.3%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 109394 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 481 596.3 0 481 7 1530 596.3 0 1530 8 38912 596.3 0 38912 9 30483 596.3 0 30483 10 80 596.3 0 80 11 27 596.3 0 27 12 35 596.3 0 35 13 31 596.3 0 31 14 34 596.3 0 34 15 33 596.3 0 33 16 44 596.3 0 44 17 34 596.3 0 34 18 34 596.3 0 34 19 31 596.3 0 31 20 20 596.3 0 20 21 46 596.3 0 46 22 41 596.3 0 41 23 47 596.3 0 47 24 40 596.3 0 40 25 42 596.3 0 42 26 29 596.3 0 29 27 29 596.3 0 29 28 43 596.3 0 43 29 30 596.3 0 30 30 31 596.3 0 31 31 22 596.3 0 22 32 42 596.3 0 42 33 39 596.3 0 39 34 35 596.3 0 35 35 34 596.3 0 34 36 36 596.3 0 36 37 60 596.3 0 60 38 121 596.3 0 121 39 52 596.3 0 52 40 74 596.3 0 74 41 45 596.3 0 45 42 42 596.3 0 42 43 27 596.3 0 27 44 38 596.3 0 38 45 33 596.3 0 33 46 27 596.3 0 27 47 37 596.3 0 37 48 42 596.3 0 42 49 22 596.3 0 22 50 40 596.3 0 40 51 41 596.3 0 41 52 54 596.3 0 54 53 33 596.3 0 33 54 31 596.3 0 31 55 74 596.3 0 74 56 38 596.3 0 38 57 46 596.3 0 46 58 46 596.3 0 46 59 24 596.3 0 24 60 61 596.3 0 61 61 43 596.3 0 43 62 39 596.3 0 39 63 51 596.3 0 51 64 46 596.3 0 46 65 40 596.3 0 40 66 41 596.3 0 41 67 56 596.3 0 56 68 64 596.3 0 64 69 47 596.3 0 47 70 42 596.3 0 42 71 57 596.3 0 57 72 33 596.3 0 33 73 34 596.3 0 34 74 69 596.3 0 69 75 68 596.3 0 68 76 63 596.3 0 63 77 29 596.3 0 29 78 68 596.3 0 68 79 51 596.3 0 51 80 66 596.3 0 66 81 39 596.3 0 39 82 40 596.3 0 40 83 52 596.3 0 52 84 50 596.3 0 50 85 44 596.3 0 44 86 53 596.3 0 53 87 57 596.3 0 57 88 58 596.3 0 58 89 59 596.3 0 59 90 61 596.3 0 61 91 134 596.3 0 134 92 69 596.3 0 69 93 66 596.3 0 66 94 128 596.3 0 128 95 75 596.3 0 75 96 79 596.3 0 79 97 487 596.3 0 487 98 506 596.3 0 506 99 662 596.3 0 662 100 348 596.3 0 348 101 42 596.3 0 42 102 31 596.3 0 31 103 36 596.3 0 36 104 47 596.3 0 47 105 52 596.3 0 52 106 64 596.3 0 64 107 72 596.3 0 72 108 94 596.3 0 94 109 127 596.3 0 127 110 91 596.3 0 91 111 652 596.3 0 652 112 516 596.3 0 516 113 540 596.3 0 540 114 44 596.3 0 44 115 43 596.3 0 43 116 54 596.3 0 54 117 39 596.3 0 39 118 43 596.3 0 43 119 45 596.3 0 45 120 38 596.3 0 38 121 30 596.3 0 30 122 42 596.3 0 42 123 42 596.3 0 42 124 32 596.3 0 32 125 59 596.3 0 59 126 67 596.3 0 67 127 44 596.3 0 44 128 46 596.3 0 46 129 72 596.3 0 72 130 93 596.3 0 93 131 100 596.3 0 100 132 187 596.3 0 187 133 261 596.3 0 261 134 498 596.3 0 498 135 148 596.3 0 148 136 93 596.3 0 93 137 64 596.3 0 64 138 51 596.3 0 51 139 50 596.3 0 50 140 91 596.3 0 91 141 98 596.3 0 98 142 60 596.3 0 60 143 40 596.3 0 40 144 65 596.3 0 65 145 64 596.3 0 64 146 215 596.3 0 215 147 110 596.3 0 110 148 116 596.3 0 116 149 64 596.3 0 64 150 57 596.3 0 57 151 61 596.3 0 61 152 54 596.3 0 54 153 63 596.3 0 63 154 153 596.3 0 153 155 253 596.3 0 253 156 384 596.3 0 384 157 208 596.3 0 208 158 56 596.3 0 56 159 43 596.3 0 43 160 39 596.3 0 39 161 45 596.3 0 45 162 51 596.3 0 51 163 46 596.3 0 46 164 61 596.3 0 61 165 42 596.3 0 42 166 198 596.3 0 198 167 151 596.3 0 151 168 184 596.3 0 184 169 47 596.3 0 47 170 40 596.3 0 40 171 40 596.3 0 40 172 47 596.3 0 47 173 31 596.3 0 31 174 53 596.3 0 53 175 54 596.3 0 54 176 65 596.3 0 65 177 50 596.3 0 50 178 50 596.3 0 50 179 57 596.3 0 57 180 46 596.3 0 46 181 56 596.3 0 56 182 57 596.3 0 57 183 45 596.3 0 45 184 37 596.3 0 37 185 72 596.3 0 72 186 60 596.3 0 60 187 47 596.3 0 47 188 59 596.3 0 59 189 61 596.3 0 61 190 65 596.3 0 65 191 56 596.3 0 56 192 67 596.3 0 67 193 227 596.3 0 227 194 792 596.3 0 792 195 664 596.3 0 664 196 691 596.3 0 691 197 137 596.3 0 137 198 72 596.3 0 72 199 91 596.3 0 91 200 170 596.3 0 170 201 205 596.3 0 205 202 182 596.3 0 182 203 91 596.3 0 91 204 69 596.3 0 69 205 57 596.3 0 57 206 67 596.3 0 67 207 101 596.3 0 101 208 98 596.3 0 98 209 68 596.3 0 68 210 68 596.3 0 68 211 47 596.3 0 47 212 52 596.3 0 52 213 54 596.3 0 54 214 62 596.3 0 62 215 116 596.3 0 116 216 198 596.3 0 198 217 155 596.3 0 155 218 56 596.3 0 56 219 52 596.3 0 52 220 248 596.3 0 248 221 372 596.3 0 372 222 424 596.3 0 424 223 260 596.3 0 260 224 48 596.3 0 48 225 44 596.3 0 44 226 53 596.3 0 53 227 50 596.3 0 50 228 64 596.3 0 64 229 68 596.3 0 68 230 64 596.3 0 64 231 59 596.3 0 59 232 62 596.3 0 62 233 63 596.3 0 63 234 65 596.3 0 65 235 44 596.3 0 44 236 87 596.3 0 87 237 124 596.3 0 124 238 303 596.3 0 303 239 263 596.3 0 263 240 125 596.3 0 125 241 183 596.3 0 183 242 152 596.3 0 152 243 471 596.3 0 471 244 362 596.3 0 362 245 444 596.3 0 444 246 217 596.3 0 217 247 65 596.3 0 65 248 77 596.3 0 77 249 80 596.3 0 80 250 104 596.3 0 104 251 166 596.3 0 166 252 192 596.3 0 192 253 161 596.3 0 161 254 650 596.3 0 650 255 847 596.3 0 847 256 925 596.3 0 925 257 160 596.3 0 160 258 166 596.3 0 166 259 157 596.3 0 157 260 134 596.3 0 134 261 214 596.3 0 214 262 1385 596.3 0 1385 263 1238 596.3 0 1238 264 1578 596.3 0 1578 265 123 596.3 0 123 266 44 596.3 0 44 267 45 596.3 0 45 268 74 596.3 0 74 269 113 596.3 0 113 270 101 596.3 0 101 271 128 596.3 0 128 272 104 596.3 0 104 273 76 596.3 0 76 274 87 596.3 0 87 275 144 596.3 0 144 276 119 596.3 0 119 277 81 596.3 0 81 278 84 596.3 0 84 279 108 596.3 0 108 280 218 596.3 0 218 281 165 596.3 0 165 282 180 596.3 0 180 283 156 596.3 0 156 284 77 596.3 0 77 285 107 596.3 0 107 286 86 596.3 0 86 287 69 596.3 0 69 288 116 596.3 0 116 289 172 596.3 0 172 290 193 596.3 0 193 291 193 596.3 0 193 292 110 596.3 0 110 293 558 596.3 0 558 294 455 596.3 0 455 295 166 596.3 0 166 296 120 596.3 0 120 297 89 596.3 0 89 298 88 596.3 0 88 299 104 596.3 0 104 300 171 596.3 0 171 301 150 596.3 0 150 Finished in 4.59 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 89,609 Reads with adapters: 32,560 (36.3%) Reads that were too short: 53 (0.1%) Reads written (passing filters): 32,507 (36.3%) Total basepairs processed: 24,074,678 bp Total written (filtered): 8,761,187 bp (36.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 32560 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 21 0.0 3 0 0 13 7 1 19 505 0.0 3 0 471 20 11 3 20 30985 0.0 4 29255 1395 250 66 19 21 72 0.0 4 7 57 7 1 22 6 0.0 4 1 0 1 4 23 134 0.0 4 3 2 0 106 23 24 4 0.0 4 0 0 0 0 4 52 1 0.0 4 0 0 0 0 1 61 2 0.0 4 0 0 0 0 2 62 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 101 3 0.0 4 0 0 0 0 3 115 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 13 0.0 4 0 0 0 0 13 127 754 0.0 4 0 0 0 0 754 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 1 135 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 164 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 11 0.0 4 0 0 0 1 10 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 259 2 0.0 4 0 0 0 0 2 263 2 0.0 4 0 0 0 1 1 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 282 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.20 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 32,507 Reads with adapters: 7,660 (23.6%) Reads written (passing filters): 32,507 (100.0%) Total basepairs processed: 8,761,187 bp Total written (filtered): 8,678,545 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7660 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 9.4% G: 86.1% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 507.9 0 13 4 4 127.0 0 4 5 2 31.7 0 2 8 1 0.5 0 1 9 1787 0.1 0 166 1621 10 5125 0.0 1 374 4751 11 656 0.0 1 26 630 12 24 0.0 1 0 24 13 13 0.0 1 0 13 150 1 0.0 1 1 169 1 0.0 1 1 208 8 0.0 1 8 223 20 0.0 1 19 1 224 5 0.0 1 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.