This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.47 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 98,542 (4.0%) Reads that were too short: 12,905 (0.5%) Reads written (passing filters): 85,637 (3.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 23,670,199 bp (3.2%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 98542 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 422 596.3 0 422 7 1084 596.3 0 1084 8 32528 596.3 0 32528 9 39982 596.3 0 39982 10 62 596.3 0 62 11 27 596.3 0 27 12 15 596.3 0 15 13 27 596.3 0 27 14 23 596.3 0 23 15 24 596.3 0 24 16 25 596.3 0 25 17 15 596.3 0 15 18 15 596.3 0 15 19 19 596.3 0 19 20 23 596.3 0 23 21 17 596.3 0 17 22 19 596.3 0 19 23 16 596.3 0 16 24 19 596.3 0 19 25 25 596.3 0 25 26 21 596.3 0 21 27 30 596.3 0 30 28 29 596.3 0 29 29 18 596.3 0 18 30 24 596.3 0 24 31 25 596.3 0 25 32 23 596.3 0 23 33 29 596.3 0 29 34 22 596.3 0 22 35 20 596.3 0 20 36 22 596.3 0 22 37 30 596.3 0 30 38 104 596.3 0 104 39 25 596.3 0 25 40 38 596.3 0 38 41 26 596.3 0 26 42 34 596.3 0 34 43 14 596.3 0 14 44 14 596.3 0 14 45 16 596.3 0 16 46 19 596.3 0 19 47 19 596.3 0 19 48 19 596.3 0 19 49 23 596.3 0 23 50 17 596.3 0 17 51 25 596.3 0 25 52 26 596.3 0 26 53 33 596.3 0 33 54 37 596.3 0 37 55 53 596.3 0 53 56 27 596.3 0 27 57 20 596.3 0 20 58 20 596.3 0 20 59 23 596.3 0 23 60 45 596.3 0 45 61 32 596.3 0 32 62 28 596.3 0 28 63 29 596.3 0 29 64 31 596.3 0 31 65 26 596.3 0 26 66 35 596.3 0 35 67 17 596.3 0 17 68 22 596.3 0 22 69 26 596.3 0 26 70 38 596.3 0 38 71 22 596.3 0 22 72 26 596.3 0 26 73 24 596.3 0 24 74 38 596.3 0 38 75 36 596.3 0 36 76 19 596.3 0 19 77 29 596.3 0 29 78 26 596.3 0 26 79 26 596.3 0 26 80 52 596.3 0 52 81 17 596.3 0 17 82 22 596.3 0 22 83 28 596.3 0 28 84 37 596.3 0 37 85 28 596.3 0 28 86 29 596.3 0 29 87 37 596.3 0 37 88 39 596.3 0 39 89 28 596.3 0 28 90 30 596.3 0 30 91 72 596.3 0 72 92 41 596.3 0 41 93 30 596.3 0 30 94 113 596.3 0 113 95 52 596.3 0 52 96 40 596.3 0 40 97 280 596.3 0 280 98 214 596.3 0 214 99 273 596.3 0 273 100 132 596.3 0 132 101 32 596.3 0 32 102 26 596.3 0 26 103 22 596.3 0 22 104 33 596.3 0 33 105 34 596.3 0 34 106 43 596.3 0 43 107 41 596.3 0 41 108 64 596.3 0 64 109 100 596.3 0 100 110 40 596.3 0 40 111 661 596.3 0 661 112 550 596.3 0 550 113 507 596.3 0 507 114 30 596.3 0 30 115 32 596.3 0 32 116 29 596.3 0 29 117 34 596.3 0 34 118 27 596.3 0 27 119 27 596.3 0 27 120 29 596.3 0 29 121 25 596.3 0 25 122 30 596.3 0 30 123 33 596.3 0 33 124 18 596.3 0 18 125 34 596.3 0 34 126 32 596.3 0 32 127 38 596.3 0 38 128 44 596.3 0 44 129 32 596.3 0 32 130 37 596.3 0 37 131 47 596.3 0 47 132 114 596.3 0 114 133 220 596.3 0 220 134 363 596.3 0 363 135 67 596.3 0 67 136 44 596.3 0 44 137 38 596.3 0 38 138 32 596.3 0 32 139 26 596.3 0 26 140 39 596.3 0 39 141 53 596.3 0 53 142 29 596.3 0 29 143 31 596.3 0 31 144 33 596.3 0 33 145 36 596.3 0 36 146 103 596.3 0 103 147 56 596.3 0 56 148 59 596.3 0 59 149 43 596.3 0 43 150 35 596.3 0 35 151 30 596.3 0 30 152 30 596.3 0 30 153 30 596.3 0 30 154 59 596.3 0 59 155 101 596.3 0 101 156 162 596.3 0 162 157 110 596.3 0 110 158 25 596.3 0 25 159 25 596.3 0 25 160 23 596.3 0 23 161 37 596.3 0 37 162 23 596.3 0 23 163 29 596.3 0 29 164 35 596.3 0 35 165 24 596.3 0 24 166 85 596.3 0 85 167 86 596.3 0 86 168 101 596.3 0 101 169 22 596.3 0 22 170 31 596.3 0 31 171 16 596.3 0 16 172 28 596.3 0 28 173 21 596.3 0 21 174 35 596.3 0 35 175 38 596.3 0 38 176 36 596.3 0 36 177 32 596.3 0 32 178 38 596.3 0 38 179 41 596.3 0 41 180 22 596.3 0 22 181 56 596.3 0 56 182 34 596.3 0 34 183 36 596.3 0 36 184 39 596.3 0 39 185 47 596.3 0 47 186 39 596.3 0 39 187 34 596.3 0 34 188 33 596.3 0 33 189 26 596.3 0 26 190 39 596.3 0 39 191 34 596.3 0 34 192 37 596.3 0 37 193 124 596.3 0 124 194 662 596.3 0 662 195 468 596.3 0 468 196 571 596.3 0 571 197 71 596.3 0 71 198 37 596.3 0 37 199 66 596.3 0 66 200 82 596.3 0 82 201 94 596.3 0 94 202 73 596.3 0 73 203 65 596.3 0 65 204 43 596.3 0 43 205 40 596.3 0 40 206 54 596.3 0 54 207 48 596.3 0 48 208 44 596.3 0 44 209 31 596.3 0 31 210 39 596.3 0 39 211 26 596.3 0 26 212 30 596.3 0 30 213 30 596.3 0 30 214 32 596.3 0 32 215 77 596.3 0 77 216 136 596.3 0 136 217 84 596.3 0 84 218 37 596.3 0 37 219 34 596.3 0 34 220 150 596.3 0 150 221 185 596.3 0 185 222 228 596.3 0 228 223 173 596.3 0 173 224 44 596.3 0 44 225 32 596.3 0 32 226 26 596.3 0 26 227 33 596.3 0 33 228 39 596.3 0 39 229 31 596.3 0 31 230 32 596.3 0 32 231 37 596.3 0 37 232 32 596.3 0 32 233 39 596.3 0 39 234 34 596.3 0 34 235 47 596.3 0 47 236 36 596.3 0 36 237 65 596.3 0 65 238 295 596.3 0 295 239 231 596.3 0 231 240 83 596.3 0 83 241 100 596.3 0 100 242 93 596.3 0 93 243 207 596.3 0 207 244 172 596.3 0 172 245 174 596.3 0 174 246 81 596.3 0 81 247 46 596.3 0 46 248 44 596.3 0 44 249 61 596.3 0 61 250 59 596.3 0 59 251 108 596.3 0 108 252 114 596.3 0 114 253 110 596.3 0 110 254 255 596.3 0 255 255 219 596.3 0 219 256 366 596.3 0 366 257 92 596.3 0 92 258 64 596.3 0 64 259 89 596.3 0 89 260 73 596.3 0 73 261 188 596.3 0 188 262 1113 596.3 0 1113 263 812 596.3 0 812 264 1408 596.3 0 1408 265 55 596.3 0 55 266 30 596.3 0 30 267 24 596.3 0 24 268 42 596.3 0 42 269 63 596.3 0 63 270 59 596.3 0 59 271 61 596.3 0 61 272 47 596.3 0 47 273 47 596.3 0 47 274 42 596.3 0 42 275 63 596.3 0 63 276 54 596.3 0 54 277 53 596.3 0 53 278 46 596.3 0 46 279 89 596.3 0 89 280 114 596.3 0 114 281 109 596.3 0 109 282 90 596.3 0 90 283 54 596.3 0 54 284 46 596.3 0 46 285 58 596.3 0 58 286 66 596.3 0 66 287 44 596.3 0 44 288 71 596.3 0 71 289 140 596.3 0 140 290 110 596.3 0 110 291 117 596.3 0 117 292 88 596.3 0 88 293 836 596.3 0 836 294 700 596.3 0 700 295 178 596.3 0 178 296 73 596.3 0 73 297 61 596.3 0 61 298 56 596.3 0 56 299 87 596.3 0 87 300 103 596.3 0 103 301 86 596.3 0 86 Finished in 4.13 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 85,637 Reads with adapters: 33,593 (39.2%) Reads that were too short: 1,119 (1.3%) Reads written (passing filters): 32,474 (37.9%) Total basepairs processed: 23,670,199 bp Total written (filtered): 8,958,039 bp (37.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33593 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 180 0.0 3 0 176 4 17 32107 0.0 3 31422 657 26 2 18 77 0.0 3 2 73 1 1 19 24 0.0 3 2 0 17 5 20 42 0.0 3 4 0 0 38 32 1 0.0 3 1 37 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 76 18 0.0 3 0 0 0 18 77 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 1 98 3 0.0 3 0 0 0 3 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 186 3 0.0 3 0 0 0 3 188 30 0.0 3 0 0 1 29 189 5 0.0 3 0 0 0 5 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 1 192 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 215 3 0.0 3 0 0 0 3 216 25 0.0 3 0 0 1 24 217 1028 0.0 3 0 0 19 1009 218 4 0.0 3 0 0 0 4 224 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.14 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 32,474 Reads with adapters: 433 (1.3%) Reads written (passing filters): 32,474 (100.0%) Total basepairs processed: 8,958,039 bp Total written (filtered): 8,956,680 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 433 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 83.8% C: 6.7% G: 6.9% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 378 507.4 0 378 4 51 126.9 0 51 5 3 31.7 0 3 6 1 7.9 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.