This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.54 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 200,914 (8.2%) Reads that were too short: 14,421 (0.6%) Reads written (passing filters): 186,493 (7.6%) Total basepairs processed: 735,190,694 bp Total written (filtered): 43,582,489 bp (5.9%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 200914 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 580 596.3 0 580 7 17203 596.3 0 17203 8 19143 596.3 0 19143 9 72 596.3 0 72 10 64 596.3 0 64 11 60 596.3 0 60 12 90 596.3 0 90 13 71 596.3 0 71 14 46 596.3 0 46 15 56 596.3 0 56 16 63 596.3 0 63 17 58 596.3 0 58 18 74 596.3 0 74 19 109 596.3 0 109 20 84 596.3 0 84 21 85 596.3 0 85 22 75 596.3 0 75 23 74 596.3 0 74 24 69 596.3 0 69 25 63 596.3 0 63 26 84 596.3 0 84 27 61 596.3 0 61 28 105 596.3 0 105 29 286 596.3 0 286 30 52 596.3 0 52 31 48 596.3 0 48 32 52 596.3 0 52 33 66 596.3 0 66 34 56 596.3 0 56 35 64 596.3 0 64 36 105 596.3 0 105 37 83 596.3 0 83 38 171 596.3 0 171 39 441 596.3 0 441 40 117 596.3 0 117 41 162 596.3 0 162 42 85 596.3 0 85 43 160 596.3 0 160 44 399 596.3 0 399 45 158 596.3 0 158 46 171 596.3 0 171 47 90 596.3 0 90 48 108 596.3 0 108 49 213 596.3 0 213 50 249 596.3 0 249 51 381 596.3 0 381 52 607 596.3 0 607 53 616 596.3 0 616 54 1167 596.3 0 1167 55 2271 596.3 0 2271 56 33619 596.3 0 33619 57 27556 596.3 0 27556 58 42508 596.3 0 42508 59 211 596.3 0 211 60 61 596.3 0 61 61 64 596.3 0 64 62 116 596.3 0 116 63 86 596.3 0 86 64 139 596.3 0 139 65 39 596.3 0 39 66 50 596.3 0 50 67 50 596.3 0 50 68 37 596.3 0 37 69 71 596.3 0 71 70 53 596.3 0 53 71 50 596.3 0 50 72 58 596.3 0 58 73 63 596.3 0 63 74 67 596.3 0 67 75 61 596.3 0 61 76 63 596.3 0 63 77 35 596.3 0 35 78 45 596.3 0 45 79 48 596.3 0 48 80 50 596.3 0 50 81 122 596.3 0 122 82 51 596.3 0 51 83 67 596.3 0 67 84 65 596.3 0 65 85 76 596.3 0 76 86 55 596.3 0 55 87 55 596.3 0 55 88 84 596.3 0 84 89 106 596.3 0 106 90 30 596.3 0 30 91 29 596.3 0 29 92 41 596.3 0 41 93 49 596.3 0 49 94 41 596.3 0 41 95 40 596.3 0 40 96 45 596.3 0 45 97 47 596.3 0 47 98 37 596.3 0 37 99 53 596.3 0 53 100 54 596.3 0 54 101 53 596.3 0 53 102 39 596.3 0 39 103 113 596.3 0 113 104 159 596.3 0 159 105 122 596.3 0 122 106 45 596.3 0 45 107 36 596.3 0 36 108 47 596.3 0 47 109 58 596.3 0 58 110 53 596.3 0 53 111 64 596.3 0 64 112 46 596.3 0 46 113 61 596.3 0 61 114 54 596.3 0 54 115 36 596.3 0 36 116 42 596.3 0 42 117 46 596.3 0 46 118 61 596.3 0 61 119 66 596.3 0 66 120 59 596.3 0 59 121 53 596.3 0 53 122 45 596.3 0 45 123 64 596.3 0 64 124 330 596.3 0 330 125 277 596.3 0 277 126 798 596.3 0 798 127 81 596.3 0 81 128 113 596.3 0 113 129 53 596.3 0 53 130 73 596.3 0 73 131 66 596.3 0 66 132 57 596.3 0 57 133 56 596.3 0 56 134 53 596.3 0 53 135 66 596.3 0 66 136 48 596.3 0 48 137 44 596.3 0 44 138 81 596.3 0 81 139 65 596.3 0 65 140 88 596.3 0 88 141 84 596.3 0 84 142 81 596.3 0 81 143 236 596.3 0 236 144 76 596.3 0 76 145 106 596.3 0 106 146 204 596.3 0 204 147 1237 596.3 0 1237 148 1223 596.3 0 1223 149 1259 596.3 0 1259 150 903 596.3 0 903 151 351 596.3 0 351 152 340 596.3 0 340 153 75 596.3 0 75 154 125 596.3 0 125 155 80 596.3 0 80 156 95 596.3 0 95 157 56 596.3 0 56 158 50 596.3 0 50 159 76 596.3 0 76 160 540 596.3 0 540 161 108 596.3 0 108 162 333 596.3 0 333 163 101 596.3 0 101 164 85 596.3 0 85 165 461 596.3 0 461 166 202 596.3 0 202 167 2169 596.3 0 2169 168 1548 596.3 0 1548 169 2289 596.3 0 2289 170 121 596.3 0 121 171 97 596.3 0 97 172 143 596.3 0 143 173 179 596.3 0 179 174 110 596.3 0 110 175 174 596.3 0 174 176 212 596.3 0 212 177 249 596.3 0 249 178 138 596.3 0 138 179 60 596.3 0 60 180 93 596.3 0 93 181 84 596.3 0 84 182 89 596.3 0 89 183 175 596.3 0 175 184 68 596.3 0 68 185 102 596.3 0 102 186 87 596.3 0 87 187 401 596.3 0 401 188 167 596.3 0 167 189 145 596.3 0 145 190 109 596.3 0 109 191 104 596.3 0 104 192 166 596.3 0 166 193 128 596.3 0 128 194 128 596.3 0 128 195 282 596.3 0 282 196 383 596.3 0 383 197 327 596.3 0 327 198 231 596.3 0 231 199 572 596.3 0 572 200 6027 596.3 0 6027 201 3764 596.3 0 3764 202 5777 596.3 0 5777 203 262 596.3 0 262 204 51 596.3 0 51 205 48 596.3 0 48 206 49 596.3 0 49 207 62 596.3 0 62 208 51 596.3 0 51 209 34 596.3 0 34 210 40 596.3 0 40 211 54 596.3 0 54 212 49 596.3 0 49 213 54 596.3 0 54 214 51 596.3 0 51 215 64 596.3 0 64 216 47 596.3 0 47 217 45 596.3 0 45 218 44 596.3 0 44 219 80 596.3 0 80 220 89 596.3 0 89 221 96 596.3 0 96 222 41 596.3 0 41 223 45 596.3 0 45 224 36 596.3 0 36 225 38 596.3 0 38 226 51 596.3 0 51 227 48 596.3 0 48 228 77 596.3 0 77 229 219 596.3 0 219 230 172 596.3 0 172 231 270 596.3 0 270 232 294 596.3 0 294 233 174 596.3 0 174 234 253 596.3 0 253 235 51 596.3 0 51 236 40 596.3 0 40 237 36 596.3 0 36 238 39 596.3 0 39 239 71 596.3 0 71 240 52 596.3 0 52 241 89 596.3 0 89 242 66 596.3 0 66 243 55 596.3 0 55 244 47 596.3 0 47 245 46 596.3 0 46 246 41 596.3 0 41 247 57 596.3 0 57 248 82 596.3 0 82 249 76 596.3 0 76 250 64 596.3 0 64 251 46 596.3 0 46 252 56 596.3 0 56 253 40 596.3 0 40 254 48 596.3 0 48 255 57 596.3 0 57 256 40 596.3 0 40 257 66 596.3 0 66 258 61 596.3 0 61 259 54 596.3 0 54 260 48 596.3 0 48 261 144 596.3 0 144 262 55 596.3 0 55 263 49 596.3 0 49 264 70 596.3 0 70 265 66 596.3 0 66 266 60 596.3 0 60 267 56 596.3 0 56 268 120 596.3 0 120 269 126 596.3 0 126 270 137 596.3 0 137 271 86 596.3 0 86 272 51 596.3 0 51 273 97 596.3 0 97 274 83 596.3 0 83 275 127 596.3 0 127 276 92 596.3 0 92 277 103 596.3 0 103 278 172 596.3 0 172 279 493 596.3 0 493 280 134 596.3 0 134 281 162 596.3 0 162 282 181 596.3 0 181 283 178 596.3 0 178 284 76 596.3 0 76 285 40 596.3 0 40 286 52 596.3 0 52 287 62 596.3 0 62 288 92 596.3 0 92 289 93 596.3 0 93 290 62 596.3 0 62 291 78 596.3 0 78 292 63 596.3 0 63 293 111 596.3 0 111 294 91 596.3 0 91 295 100 596.3 0 100 296 53 596.3 0 53 297 60 596.3 0 60 298 46 596.3 0 46 299 49 596.3 0 49 300 105 596.3 0 105 301 153 596.3 0 153 Finished in 7.53 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 186,493 Reads with adapters: 17,300 (9.3%) Reads that were too short: 126 (0.1%) Reads written (passing filters): 17,174 (9.2%) Total basepairs processed: 43,582,489 bp Total written (filtered): 4,749,034 bp (10.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17300 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 119 0.0 3 0 117 2 17 16925 0.0 3 16606 304 13 2 18 35 0.0 3 1 30 4 19 7 0.0 3 0 0 5 2 20 40 0.0 3 1 0 0 39 21 1 0.0 3 1 22 1 0.0 3 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 16 0.0 3 0 0 1 15 56 2 0.0 3 0 0 0 2 57 6 0.0 3 0 0 1 5 58 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 2 0.0 3 1 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 83 3 0.0 3 0 0 0 3 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 15 0.0 3 0 0 0 15 146 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 165 3 0.0 3 0 0 0 3 166 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 52 0.0 3 0 0 1 51 219 7 0.0 3 0 0 0 7 221 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 229 3 0.0 3 0 0 0 3 231 6 0.0 3 0 0 0 6 232 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 240 1 0.0 3 1 254 1 0.0 3 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 17,174 Reads with adapters: 47 (0.3%) Reads written (passing filters): 17,174 (100.0%) Total basepairs processed: 4,749,034 bp Total written (filtered): 4,748,888 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 47 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.1% C: 53.2% G: 19.1% T: 8.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 42 268.3 0 42 4 5 67.1 0 5