This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.56 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 331,400 (13.6%) Reads that were too short: 13,743 (0.6%) Reads written (passing filters): 317,657 (13.0%) Total basepairs processed: 735,190,694 bp Total written (filtered): 85,654,269 bp (11.7%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 331400 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2534 596.3 0 2534 7 81300 596.3 0 81300 8 1497 596.3 0 1497 9 99482 596.3 0 99482 10 92 596.3 0 92 11 41 596.3 0 41 12 43 596.3 0 43 13 40 596.3 0 40 14 32 596.3 0 32 15 39 596.3 0 39 16 41 596.3 0 41 17 49 596.3 0 49 18 70 596.3 0 70 19 55 596.3 0 55 20 46 596.3 0 46 21 54 596.3 0 54 22 51 596.3 0 51 23 64 596.3 0 64 24 66 596.3 0 66 25 58 596.3 0 58 26 63 596.3 0 63 27 77 596.3 0 77 28 159 596.3 0 159 29 227 596.3 0 227 30 106 596.3 0 106 31 142 596.3 0 142 32 344 596.3 0 344 33 114 596.3 0 114 34 118 596.3 0 118 35 179 596.3 0 179 36 167 596.3 0 167 37 198 596.3 0 198 38 356 596.3 0 356 39 285 596.3 0 285 40 313 596.3 0 313 41 453 596.3 0 453 42 7652 596.3 0 7652 43 12156 596.3 0 12156 44 7773 596.3 0 7773 45 88 596.3 0 88 46 56 596.3 0 56 47 126 596.3 0 126 48 293 596.3 0 293 49 90 596.3 0 90 50 164 596.3 0 164 51 97 596.3 0 97 52 114 596.3 0 114 53 316 596.3 0 316 54 141 596.3 0 141 55 140 596.3 0 140 56 84 596.3 0 84 57 122 596.3 0 122 58 179 596.3 0 179 59 222 596.3 0 222 60 381 596.3 0 381 61 616 596.3 0 616 62 698 596.3 0 698 63 1261 596.3 0 1261 64 2405 596.3 0 2405 65 29475 596.3 0 29475 66 18444 596.3 0 18444 67 37983 596.3 0 37983 68 173 596.3 0 173 69 61 596.3 0 61 70 50 596.3 0 50 71 71 596.3 0 71 72 45 596.3 0 45 73 45 596.3 0 45 74 36 596.3 0 36 75 33 596.3 0 33 76 60 596.3 0 60 77 69 596.3 0 69 78 44 596.3 0 44 79 38 596.3 0 38 80 46 596.3 0 46 81 51 596.3 0 51 82 30 596.3 0 30 83 34 596.3 0 34 84 35 596.3 0 35 85 47 596.3 0 47 86 35 596.3 0 35 87 132 596.3 0 132 88 39 596.3 0 39 89 56 596.3 0 56 90 27 596.3 0 27 91 52 596.3 0 52 92 29 596.3 0 29 93 56 596.3 0 56 94 52 596.3 0 52 95 49 596.3 0 49 96 56 596.3 0 56 97 50 596.3 0 50 98 68 596.3 0 68 99 51 596.3 0 51 100 54 596.3 0 54 101 70 596.3 0 70 102 118 596.3 0 118 103 37 596.3 0 37 104 48 596.3 0 48 105 34 596.3 0 34 106 43 596.3 0 43 107 48 596.3 0 48 108 44 596.3 0 44 109 40 596.3 0 40 110 52 596.3 0 52 111 59 596.3 0 59 112 48 596.3 0 48 113 25 596.3 0 25 114 40 596.3 0 40 115 43 596.3 0 43 116 28 596.3 0 28 117 32 596.3 0 32 118 42 596.3 0 42 119 49 596.3 0 49 120 33 596.3 0 33 121 44 596.3 0 44 122 44 596.3 0 44 123 41 596.3 0 41 124 48 596.3 0 48 125 28 596.3 0 28 126 39 596.3 0 39 127 49 596.3 0 49 128 44 596.3 0 44 129 46 596.3 0 46 130 50 596.3 0 50 131 34 596.3 0 34 132 58 596.3 0 58 133 60 596.3 0 60 134 204 596.3 0 204 135 257 596.3 0 257 136 171 596.3 0 171 137 97 596.3 0 97 138 477 596.3 0 477 139 242 596.3 0 242 140 66 596.3 0 66 141 41 596.3 0 41 142 32 596.3 0 32 143 46 596.3 0 46 144 44 596.3 0 44 145 38 596.3 0 38 146 38 596.3 0 38 147 45 596.3 0 45 148 60 596.3 0 60 149 46 596.3 0 46 150 28 596.3 0 28 151 29 596.3 0 29 152 34 596.3 0 34 153 41 596.3 0 41 154 38 596.3 0 38 155 41 596.3 0 41 156 45 596.3 0 45 157 52 596.3 0 52 158 40 596.3 0 40 159 55 596.3 0 55 160 54 596.3 0 54 161 46 596.3 0 46 162 26 596.3 0 26 163 34 596.3 0 34 164 32 596.3 0 32 165 31 596.3 0 31 166 33 596.3 0 33 167 46 596.3 0 46 168 48 596.3 0 48 169 40 596.3 0 40 170 37 596.3 0 37 171 30 596.3 0 30 172 38 596.3 0 38 173 37 596.3 0 37 174 45 596.3 0 45 175 39 596.3 0 39 176 35 596.3 0 35 177 35 596.3 0 35 178 55 596.3 0 55 179 73 596.3 0 73 180 74 596.3 0 74 181 70 596.3 0 70 182 58 596.3 0 58 183 60 596.3 0 60 184 87 596.3 0 87 185 71 596.3 0 71 186 93 596.3 0 93 187 32 596.3 0 32 188 33 596.3 0 33 189 39 596.3 0 39 190 40 596.3 0 40 191 52 596.3 0 52 192 37 596.3 0 37 193 30 596.3 0 30 194 30 596.3 0 30 195 28 596.3 0 28 196 46 596.3 0 46 197 69 596.3 0 69 198 86 596.3 0 86 199 58 596.3 0 58 200 53 596.3 0 53 201 67 596.3 0 67 202 106 596.3 0 106 203 100 596.3 0 100 204 57 596.3 0 57 205 42 596.3 0 42 206 35 596.3 0 35 207 52 596.3 0 52 208 47 596.3 0 47 209 62 596.3 0 62 210 52 596.3 0 52 211 49 596.3 0 49 212 57 596.3 0 57 213 38 596.3 0 38 214 37 596.3 0 37 215 77 596.3 0 77 216 82 596.3 0 82 217 69 596.3 0 69 218 84 596.3 0 84 219 79 596.3 0 79 220 74 596.3 0 74 221 77 596.3 0 77 222 363 596.3 0 363 223 495 596.3 0 495 224 444 596.3 0 444 225 270 596.3 0 270 226 38 596.3 0 38 227 48 596.3 0 48 228 66 596.3 0 66 229 49 596.3 0 49 230 41 596.3 0 41 231 43 596.3 0 43 232 42 596.3 0 42 233 45 596.3 0 45 234 48 596.3 0 48 235 43 596.3 0 43 236 52 596.3 0 52 237 45 596.3 0 45 238 68 596.3 0 68 239 47 596.3 0 47 240 73 596.3 0 73 241 73 596.3 0 73 242 151 596.3 0 151 243 297 596.3 0 297 244 213 596.3 0 213 245 131 596.3 0 131 246 44 596.3 0 44 247 45 596.3 0 45 248 51 596.3 0 51 249 63 596.3 0 63 250 119 596.3 0 119 251 98 596.3 0 98 252 111 596.3 0 111 253 181 596.3 0 181 254 204 596.3 0 204 255 1127 596.3 0 1127 256 934 596.3 0 934 257 1246 596.3 0 1246 258 276 596.3 0 276 259 165 596.3 0 165 260 400 596.3 0 400 261 191 596.3 0 191 262 321 596.3 0 321 263 117 596.3 0 117 264 122 596.3 0 122 265 185 596.3 0 185 266 183 596.3 0 183 267 85 596.3 0 85 268 71 596.3 0 71 269 70 596.3 0 70 270 387 596.3 0 387 271 200 596.3 0 200 272 227 596.3 0 227 273 60 596.3 0 60 274 90 596.3 0 90 275 52 596.3 0 52 276 87 596.3 0 87 277 102 596.3 0 102 278 129 596.3 0 129 279 150 596.3 0 150 280 74 596.3 0 74 281 44 596.3 0 44 282 45 596.3 0 45 283 46 596.3 0 46 284 49 596.3 0 49 285 34 596.3 0 34 286 43 596.3 0 43 287 58 596.3 0 58 288 46 596.3 0 46 289 33 596.3 0 33 290 45 596.3 0 45 291 61 596.3 0 61 292 77 596.3 0 77 293 74 596.3 0 74 294 81 596.3 0 81 295 37 596.3 0 37 296 40 596.3 0 40 297 67 596.3 0 67 298 52 596.3 0 52 299 92 596.3 0 92 300 233 596.3 0 233 301 58 596.3 0 58 Finished in 15.64 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 317,657 Reads with adapters: 85,341 (26.9%) Reads that were too short: 251 (0.1%) Reads written (passing filters): 85,090 (26.8%) Total basepairs processed: 85,654,269 bp Total written (filtered): 22,931,696 bp (26.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 85341 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 27 0.0 3 0 0 25 2 19 885 0.0 3 0 765 77 32 11 20 80164 0.0 4 75359 3994 637 136 38 21 155 0.0 4 29 122 4 22 30 0.0 4 3 2 18 3 4 23 325 0.0 4 176 3 2 112 32 24 5 0.0 4 1 1 0 0 3 26 2 0.0 4 0 0 0 0 2 30 1 0.0 4 1 34 2 0.0 4 0 0 0 0 2 37 1 0.0 4 0 0 0 0 1 57 1 0.0 4 1 64 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 1 92 26 0.0 4 0 0 0 0 26 95 1 0.0 4 0 0 0 1 106 1 0.0 4 0 0 0 0 1 107 26 0.0 4 0 0 0 0 26 113 43 0.0 4 0 0 0 0 43 114 4 0.0 4 0 0 0 1 3 115 3 0.0 4 0 0 0 0 3 125 6 0.0 4 0 0 0 0 6 126 45 0.0 4 0 0 0 0 45 127 3302 0.0 4 0 0 0 0 3302 128 5 0.0 4 0 0 0 0 5 130 19 0.0 4 0 0 0 0 19 144 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 2 0.0 4 0 0 0 0 2 168 3 0.0 4 0 0 0 0 3 181 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 187 1 0.0 4 0 0 0 0 1 191 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 193 101 0.0 4 0 0 0 4 97 194 38 0.0 4 0 0 0 2 36 195 4 0.0 4 0 0 0 0 4 196 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 206 1 0.0 4 0 0 0 0 1 207 2 0.0 4 0 0 0 0 2 210 3 0.0 4 0 0 0 0 3 213 1 0.0 4 0 0 0 0 1 214 3 0.0 4 0 0 0 1 2 215 3 0.0 4 0 0 0 0 3 216 1 0.0 4 0 0 0 0 1 217 2 0.0 4 0 0 0 0 2 218 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 222 2 0.0 4 0 0 0 0 2 228 16 0.0 4 0 0 0 1 15 229 11 0.0 4 0 0 0 1 10 230 4 0.0 4 0 0 0 0 4 234 4 0.0 4 0 0 0 0 4 236 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 244 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 258 2 0.0 4 0 0 0 0 2 259 2 0.0 4 0 0 0 1 1 261 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 3 0.0 4 0 0 0 0 3 270 3 0.0 4 0 0 0 0 3 274 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 283 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.96 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 85,090 Reads with adapters: 6,567 (7.7%) Reads written (passing filters): 85,090 (100.0%) Total basepairs processed: 22,931,696 bp Total written (filtered): 22,810,431 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6567 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 14.6% G: 77.1% T: 2.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 369 1329.5 0 369 4 30 332.4 0 30 9 27 0.3 0 1 26 10 110 0.1 1 5 105 11 4450 0.0 1 203 4247 12 1341 0.0 1 26 1315 13 23 0.0 1 1 22 15 6 0.0 1 0 6 16 1 0.0 1 0 1 49 1 0.0 1 0 1 152 9 0.0 1 5 4 191 1 0.0 1 0 1 210 12 0.0 1 11 1 225 5 0.0 1 5 230 1 0.0 1 0 1 257 1 0.0 1 1 259 3 0.0 1 2 1 262 1 0.0 1 1 264 171 0.0 1 158 13 265 5 0.0 1 4 1