This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.36 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 339,145 (13.9%) Reads that were too short: 18,686 (0.8%) Reads written (passing filters): 320,459 (13.1%) Total basepairs processed: 735,190,694 bp Total written (filtered): 91,066,051 bp (12.4%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 339145 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2096 596.3 0 2096 7 2864 596.3 0 2864 8 161366 596.3 0 161366 9 131669 596.3 0 131669 10 168 596.3 0 168 11 52 596.3 0 52 12 53 596.3 0 53 13 44 596.3 0 44 14 40 596.3 0 40 15 24 596.3 0 24 16 34 596.3 0 34 17 49 596.3 0 49 18 39 596.3 0 39 19 56 596.3 0 56 20 50 596.3 0 50 21 40 596.3 0 40 22 48 596.3 0 48 23 35 596.3 0 35 24 191 596.3 0 191 25 53 596.3 0 53 26 42 596.3 0 42 27 33 596.3 0 33 28 47 596.3 0 47 29 39 596.3 0 39 30 51 596.3 0 51 31 49 596.3 0 49 32 25 596.3 0 25 33 33 596.3 0 33 34 35 596.3 0 35 35 32 596.3 0 32 36 101 596.3 0 101 37 32 596.3 0 32 38 29 596.3 0 29 39 48 596.3 0 48 40 40 596.3 0 40 41 32 596.3 0 32 42 24 596.3 0 24 43 43 596.3 0 43 44 40 596.3 0 40 45 37 596.3 0 37 46 32 596.3 0 32 47 30 596.3 0 30 48 33 596.3 0 33 49 31 596.3 0 31 50 40 596.3 0 40 51 30 596.3 0 30 52 38 596.3 0 38 53 41 596.3 0 41 54 47 596.3 0 47 55 41 596.3 0 41 56 34 596.3 0 34 57 38 596.3 0 38 58 50 596.3 0 50 59 34 596.3 0 34 60 31 596.3 0 31 61 21 596.3 0 21 62 25 596.3 0 25 63 46 596.3 0 46 64 36 596.3 0 36 65 67 596.3 0 67 66 51 596.3 0 51 67 69 596.3 0 69 68 33 596.3 0 33 69 100 596.3 0 100 70 71 596.3 0 71 71 157 596.3 0 157 72 44 596.3 0 44 73 40 596.3 0 40 74 34 596.3 0 34 75 54 596.3 0 54 76 71 596.3 0 71 77 98 596.3 0 98 78 149 596.3 0 149 79 102 596.3 0 102 80 477 596.3 0 477 81 136 596.3 0 136 82 176 596.3 0 176 83 78 596.3 0 78 84 54 596.3 0 54 85 58 596.3 0 58 86 92 596.3 0 92 87 56 596.3 0 56 88 35 596.3 0 35 89 73 596.3 0 73 90 68 596.3 0 68 91 64 596.3 0 64 92 63 596.3 0 63 93 81 596.3 0 81 94 74 596.3 0 74 95 71 596.3 0 71 96 1068 596.3 0 1068 97 896 596.3 0 896 98 1204 596.3 0 1204 99 93 596.3 0 93 100 84 596.3 0 84 101 50 596.3 0 50 102 45 596.3 0 45 103 42 596.3 0 42 104 41 596.3 0 41 105 44 596.3 0 44 106 46 596.3 0 46 107 38 596.3 0 38 108 37 596.3 0 37 109 55 596.3 0 55 110 71 596.3 0 71 111 48 596.3 0 48 112 66 596.3 0 66 113 59 596.3 0 59 114 78 596.3 0 78 115 48 596.3 0 48 116 37 596.3 0 37 117 44 596.3 0 44 118 52 596.3 0 52 119 45 596.3 0 45 120 52 596.3 0 52 121 37 596.3 0 37 122 55 596.3 0 55 123 40 596.3 0 40 124 48 596.3 0 48 125 56 596.3 0 56 126 71 596.3 0 71 127 86 596.3 0 86 128 135 596.3 0 135 129 139 596.3 0 139 130 431 596.3 0 431 131 204 596.3 0 204 132 291 596.3 0 291 133 240 596.3 0 240 134 221 596.3 0 221 135 67 596.3 0 67 136 56 596.3 0 56 137 91 596.3 0 91 138 101 596.3 0 101 139 201 596.3 0 201 140 80 596.3 0 80 141 72 596.3 0 72 142 74 596.3 0 74 143 60 596.3 0 60 144 71 596.3 0 71 145 66 596.3 0 66 146 72 596.3 0 72 147 92 596.3 0 92 148 1225 596.3 0 1225 149 329 596.3 0 329 150 245 596.3 0 245 151 54 596.3 0 54 152 52 596.3 0 52 153 43 596.3 0 43 154 73 596.3 0 73 155 58 596.3 0 58 156 49 596.3 0 49 157 70 596.3 0 70 158 70 596.3 0 70 159 70 596.3 0 70 160 53 596.3 0 53 161 55 596.3 0 55 162 38 596.3 0 38 163 43 596.3 0 43 164 46 596.3 0 46 165 49 596.3 0 49 166 44 596.3 0 44 167 54 596.3 0 54 168 38 596.3 0 38 169 49 596.3 0 49 170 41 596.3 0 41 171 40 596.3 0 40 172 48 596.3 0 48 173 57 596.3 0 57 174 58 596.3 0 58 175 44 596.3 0 44 176 47 596.3 0 47 177 57 596.3 0 57 178 75 596.3 0 75 179 49 596.3 0 49 180 53 596.3 0 53 181 70 596.3 0 70 182 54 596.3 0 54 183 81 596.3 0 81 184 105 596.3 0 105 185 167 596.3 0 167 186 213 596.3 0 213 187 1324 596.3 0 1324 188 2486 596.3 0 2486 189 888 596.3 0 888 190 380 596.3 0 380 191 49 596.3 0 49 192 72 596.3 0 72 193 41 596.3 0 41 194 67 596.3 0 67 195 62 596.3 0 62 196 48 596.3 0 48 197 43 596.3 0 43 198 49 596.3 0 49 199 36 596.3 0 36 200 52 596.3 0 52 201 39 596.3 0 39 202 53 596.3 0 53 203 59 596.3 0 59 204 63 596.3 0 63 205 79 596.3 0 79 206 74 596.3 0 74 207 91 596.3 0 91 208 75 596.3 0 75 209 426 596.3 0 426 210 561 596.3 0 561 211 606 596.3 0 606 212 141 596.3 0 141 213 69 596.3 0 69 214 59 596.3 0 59 215 53 596.3 0 53 216 56 596.3 0 56 217 55 596.3 0 55 218 62 596.3 0 62 219 68 596.3 0 68 220 65 596.3 0 65 221 57 596.3 0 57 222 62 596.3 0 62 223 100 596.3 0 100 224 90 596.3 0 90 225 95 596.3 0 95 226 209 596.3 0 209 227 134 596.3 0 134 228 101 596.3 0 101 229 83 596.3 0 83 230 114 596.3 0 114 231 89 596.3 0 89 232 107 596.3 0 107 233 145 596.3 0 145 234 152 596.3 0 152 235 266 596.3 0 266 236 186 596.3 0 186 237 125 596.3 0 125 238 114 596.3 0 114 239 131 596.3 0 131 240 419 596.3 0 419 241 494 596.3 0 494 242 937 596.3 0 937 243 508 596.3 0 508 244 220 596.3 0 220 245 114 596.3 0 114 246 302 596.3 0 302 247 179 596.3 0 179 248 163 596.3 0 163 249 335 596.3 0 335 250 315 596.3 0 315 251 389 596.3 0 389 252 244 596.3 0 244 253 515 596.3 0 515 254 1082 596.3 0 1082 255 881 596.3 0 881 256 252 596.3 0 252 257 112 596.3 0 112 258 174 596.3 0 174 259 146 596.3 0 146 260 315 596.3 0 315 261 293 596.3 0 293 262 550 596.3 0 550 263 117 596.3 0 117 264 69 596.3 0 69 265 137 596.3 0 137 266 122 596.3 0 122 267 142 596.3 0 142 268 165 596.3 0 165 269 148 596.3 0 148 270 241 596.3 0 241 271 310 596.3 0 310 272 362 596.3 0 362 273 310 596.3 0 310 274 441 596.3 0 441 275 198 596.3 0 198 276 47 596.3 0 47 277 34 596.3 0 34 278 56 596.3 0 56 279 75 596.3 0 75 280 58 596.3 0 58 281 83 596.3 0 83 282 88 596.3 0 88 283 192 596.3 0 192 284 119 596.3 0 119 285 71 596.3 0 71 286 47 596.3 0 47 287 44 596.3 0 44 288 42 596.3 0 42 289 44 596.3 0 44 290 49 596.3 0 49 291 44 596.3 0 44 292 72 596.3 0 72 293 88 596.3 0 88 294 95 596.3 0 95 295 69 596.3 0 69 296 73 596.3 0 73 297 75 596.3 0 75 298 86 596.3 0 86 299 55 596.3 0 55 300 63 596.3 0 63 301 141 596.3 0 141 Finished in 15.75 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 320,459 Reads with adapters: 136,539 (42.6%) Reads that were too short: 208 (0.1%) Reads written (passing filters): 136,331 (42.5%) Total basepairs processed: 91,066,051 bp Total written (filtered): 37,027,535 bp (40.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 136539 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 6 4 18 89 0.0 3 0 0 58 20 11 19 1779 0.0 3 0 1589 106 63 21 20 133029 0.0 4 125232 6264 1155 293 85 21 272 0.0 4 31 214 15 8 4 22 21 0.0 4 1 1 8 5 6 23 546 0.0 4 2 1 1 426 116 24 8 0.0 4 4 0 0 0 4 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 1 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 32 3 0.0 4 1 0 0 0 2 41 4 0.0 4 0 0 0 0 4 42 6 0.0 4 1 0 0 1 4 43 2 0.0 4 0 0 0 0 2 44 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 54 3 0.0 4 0 0 0 0 3 56 12 0.0 4 0 0 0 0 12 58 17 0.0 4 0 0 0 0 17 60 1 0.0 4 0 0 0 0 1 87 4 0.0 4 0 0 0 0 4 88 3 0.0 4 0 0 0 0 3 91 1 0.0 4 0 0 0 0 1 92 8 0.0 4 0 0 0 0 8 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 110 1 0.0 4 1 111 4 0.0 4 0 0 0 1 3 112 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 7 0.0 4 0 0 0 0 7 127 503 0.0 4 0 0 0 0 503 128 3 0.0 4 0 0 0 0 3 130 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 0 2 139 10 0.0 4 0 0 0 0 10 149 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 159 1 0.0 4 0 1 171 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 202 3 0.0 4 1 0 0 0 2 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 2 0.0 4 0 0 0 0 2 216 1 0.0 4 0 0 0 1 217 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 2 0.0 4 0 0 0 0 2 225 2 0.0 4 0 0 0 0 2 226 2 0.0 4 0 0 0 0 2 227 3 0.0 4 0 0 0 0 3 228 60 0.0 4 0 0 0 1 59 229 21 0.0 4 0 0 0 0 21 230 8 0.0 4 0 0 1 0 7 231 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 235 4 0.0 4 0 0 0 0 4 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 0 1 254 2 0.0 4 0 0 0 0 2 257 5 0.0 4 0 0 0 0 5 263 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 0 3 265 1 0.0 4 0 0 0 0 1 267 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 282 8 0.0 4 0 0 0 0 8 284 2 0.0 4 0 0 0 0 2 288 2 0.0 4 0 0 0 0 2 291 2 0.0 4 0 0 0 0 2 292 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.01 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 136,331 Reads with adapters: 32,869 (24.1%) Reads written (passing filters): 136,331 (100.0%) Total basepairs processed: 37,027,535 bp Total written (filtered): 36,686,006 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 32869 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 9.5% G: 86.0% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 42 2130.2 0 42 4 26 532.5 0 26 5 4 133.1 0 4 6 12 33.3 0 12 7 8 8.3 0 8 8 64 2.1 0 64 9 9594 0.5 0 896 8698 10 19630 0.1 1 1502 18128 11 3188 0.0 1 142 3046 12 111 0.0 1 2 109 13 78 0.0 1 0 78 14 4 0.0 1 0 4 82 1 0.0 1 1 87 1 0.0 1 1 150 36 0.0 1 33 3 151 1 0.0 1 1 152 1 0.0 1 1 189 1 0.0 1 0 1 190 1 0.0 1 0 1 208 30 0.0 1 26 4 209 1 0.0 1 1 210 1 0.0 1 1 220 1 0.0 1 0 1 223 30 0.0 1 26 4 226 2 0.0 1 1 1 251 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.